LOCUS       sequence-370066-       18144 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mammalian cell expression of 3xFLAG-HUWE1 H3962D.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 18144)
  AUTHORS   Hunkeler M, Jin CY, Ma MW, Monda JK, Overwijn D, Bennett EJ, Fischer
            ES
  TITLE     Solenoid architecture of HUWE1 contributes to ligase activity and 
            substrate recognition.
  JOURNAL   Mol Cell. 2021 Sep 2;81(17):3468-3480.e7. doi: 
            10.1016/j.molcel.2021.06.032. Epub 2021 Jul 26.
  PUBMED    34314700
REFERENCE   2  (bases 1 to 18144)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2021.06.032"; journalName: "Mol Cell"; date: 
            "2021-09-2- 2"; volume: "81"; issue: "17"; pages: "3468-3480.e7"
COMMENT     Sequence Label: pDARMO_CMVT_3xFLAG-HUWE1_H3962D
FEATURES             Location/Qualifiers
     source          1..18144
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     mobile_element  1..224
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     rep_origin      complement(288..876)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(368..387)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(915..1706,1707..1775))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1538..1557
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1776..1847)
                     /gene="bla"
                     /label=AmpR promoter
     mobile_element  1872..2037
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     enhancer        2131..2434
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2435..2638
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2589..2609
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     3140..3159
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      3212..3221
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3224..3289
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             3308..3331
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     primer_bind     complement(16571..16591)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(16989..17000)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(17067..17084)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    17073..17280
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     promoter        join(17328..17333,17334..17350,17351..17356)
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     CDS             17545..18078
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
ORIGIN
        1 gaatatagtc tttacatgcc cttggccacg cccctcttta atacgacggg caatttgcac
       61 ttcagaaaat gaagagtttg ctttagccat aacaaaagtc cagtatgctt tttcacagca
      121 taactggact gatttcagtt tacaactatt ctgtctagtt taagacttta ttgtcatagt
      181 ttagatctat tttgttcagt ttaagacttt attgtccgcc cacacccgct tacgcagggc
      241 atctgcgcat ccacaggaag ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
      301 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
      361 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
      421 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
      481 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
      541 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
      601 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
      661 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
      721 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
      781 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
      841 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
      901 gggtctgacg cttattacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
      961 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     1021 ttaccatctg gccccagtgc tgcaatgata ccgcgtgacc cacgctcacc ggctccagat
     1081 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     1141 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     1201 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
     1261 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
     1321 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
     1381 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
     1441 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
     1501 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
     1561 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
     1621 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
     1681 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
     1741 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
     1801 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttaggaccca
     1861 agtcaaccgg ttgtgggcgg acaaaatagt tgggaactgg gaggggtgga aatggagttt
     1921 ttaaggatta tttagggaag agtgacaaaa tagatgggaa ctgggtgtag cgtcgtaagc
     1981 taatacgaaa attaaaaatg acaaaatagt ttggaactag atttcactta tctggttcag
     2041 cctgcggtcc ggattcgccc ttaagctagc tagttattaa tagtaatcaa ttacggggtc
     2101 attagttcat agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc
     2161 tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt
     2221 aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca
     2281 cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg
     2341 taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca
     2401 gtacatctac gtattagtca ttgctattac catggtgatg cggttttggc agtacatcaa
     2461 tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa
     2521 tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc
     2581 cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctgg
     2641 tttagtgaac cgtcagatcc tcactctctt ccgcctcgct gtctgcgagg gccagctgtt
     2701 gggctcgcgg ttgaggacaa actcttcgcg gtctttccag tactcttgga tcggaaaccc
     2761 gtcggcctcc gaacggtact ccgccaccga gggacctgag cgagtccgca tcgaccggat
     2821 cggaaaacct ctcgagaaag gcgtctaacc agtcacagtc gcaaggtagg ctgagcaccg
     2881 tggcgggcgg cagcgggtgg cggtcggggt tgtttctggc ggaggtgctg ctgatgatgt
     2941 aattaaagta ggcggtgttg acaccgcgga tggtcgaggt gaggtgtggc aggcttgaga
     3001 tccagctgtt ggggtgagta ctccctctca aaagcgggca ttacttctgc gctaagattg
     3061 tcagtttcca aaaacgagga ggatttgata ttcacctggc ccgatctggc catacacttg
     3121 agtgacaatg acatccactt tgcctttctc tccacaggtg tccactccca ggtccaaagt
     3181 tggccaggcc tagttggcca ggcctagtgc cgccaccatg ggtgactaca aagaccatga
     3241 cggtgattat aaagatcatg acattgatta caaggatgac gatgacaagg gcggtggtag
     3301 cggtgggctg gaagttctgt tccaggggcc cgtcgacacc atgaaagtag acaggactaa
     3361 actgaagaag acacctactg aggctcctgc agactgcaga gccttaatag acaaactcaa
     3421 agtttgtaat gatgagcaac ttctcttgga actgcagcag atcaaaacat ggaacattgg
     3481 aaagtgcgag ttatatcact gggtggacct gttggaccgc ttcgatggaa tactggcaga
     3541 tgctggacag acagtggaga atatgtcatg gatgctcgta tgtgataggc cagaaagaga
     3601 gcaactgaaa atgcttctct tggctgtgtt gaacttcaca gccttgctca ttgagtacag
     3661 cttttcccgg catctgtaca gttccataga gcatttgaca actttattgg cttcctctga
     3721 tatgcaagtg gtgctggcag tcctcaatct cctatatgta tttagcaaaa gatcaaacta
     3781 catcactcgt ctgggatctg acaagaggac cccgctgcta actcggctac aacatttggc
     3841 agagagctgg ggtggaaagg agaatggctt tggacttgca gaatgttgca gagacttgca
     3901 tatgatgaaa tatccaccca gtgcaactac actacacttt gaattctatg cagatcctgg
     3961 ggccgaggtc aaaattgaga aaaggacaac tagtaacaca ctacattata ttcacataga
     4021 gcaacttgac aagatttcag aaagcccttc tgaaatcatg gaatctctta ccaaaatgta
     4081 cagcattcct aaggataagc agatgctgtt atttacacac atacgactgg cccatggctt
     4141 ttctaatcac aggaagcgat tgcaggcagt tcaggccaga ctgcatgcaa tatctatatt
     4201 agtgtattcc aatgccttgc aggaatcagc aaacagtatc ttgtataatg gcttgataga
     4261 ggagttagtg gacgtgctgc aaataacgga taagcagctt atggagatta aagcagcttc
     4321 tttacgaaca ttaacatcaa ttgtccactt ggagagaact cccaaactca gcagtattat
     4381 tgactgtact ggaactgcct cctaccatgg atttttgcca gtgcttgtaa ggaactgtat
     4441 ccaggccatg attgatcctt ccatggatcc ataccctcac cagtttgcca ctgctctctt
     4501 ctctttttta taccatctgg ccagctacga tgctggtggt gaagccttgg tctcctgtgg
     4561 aatgatggaa gccttattga aggtcataaa gtttcttggc gatgaacagg accagataac
     4621 atttgtcacc agagccgtca gagtggttga ccttatcacc aacctggata tggcagcttt
     4681 tcaatcccat agtggacttt ctatcttcat ttatagactt gagcatgaag tagatctgtg
     4741 ccgaaaagaa tgtccgtttg tgatcaagcc aaagatccag agacccaata ctacacaaga
     4801 aggagaggaa atggaaactg atatggatgg agtccagtgt attccacaac gagcagcact
     4861 tctgaaatcc atgttgaatt tcctcaagaa ggccatccaa gaccctgctt tctcagatgg
     4921 catacgacat gtgatggatg gttctctgcc tacctccctg aaacacatca tcagcaatgc
     4981 agaatactat ggcccatcac tcttcctcct agctactgaa gtggtgactg tgtttgtatt
     5041 tcaagaacca tcactgctct cctcactcca ggacaatgga ttgacagatg tcatgctgca
     5101 tgcactgctt atcaaagatg ttcctgctac ccgtgaagtc cttggctccc tcccaaatgt
     5161 attcagtgca ctctgtttga atgcccgagg tcttcagtct tttgttcagt gtcagccttt
     5221 tgaacgcctc ttcaaagttc ttctgtctcc agattacctc ccagccatgc ggaggaggag
     5281 aagttctgat ccccttgggg atactgcatc caacctgggg agtgctgtcg atgagctcat
     5341 gagacatcag cccaccctta aaacagatgc aacgactgcc atcatcaagt tacttgaaga
     5401 aatctgtaat cttggaaggg accccaaata catctgtcag aagccatcga tccagaaggc
     5461 agatggcact gccactgctc ctcccccaag gtctaatcat gccgcagaag aagcctctag
     5521 tgaggatgag gaggaagagg aagtacaggc catgcagagc tttaattcta cccagcaaaa
     5581 tgaaactgag cctaatcagc aggttgttgg tacagaggaa cgtattccta ttcccctcat
     5641 ggattacatc cttaatgtga tgaaatttgt ggaatctatt ctgagcaaca atacaacaga
     5701 tgaccactgc caggaatttg tgaatcagaa aggactgttg cctttggtta ccattttggg
     5761 tcttcccaat ctgcccattg actttcccac atctgctgcc tgtcaggctg ttgcaggtgt
     5821 ctgcaaatcc atattgacac tgtcacatga acccaaagtc cttcaagagg gtctccttca
     5881 gttggactcc atcctctcct ccctggagcc cttacaccgc cccattgaat cccctggggg
     5941 ctcagtgttg ttgcgagaac tggcttgcgc aggcaatgtt gctgatgcta ccctctcagc
     6001 ccaggccaca cctctgctgc atgcactcac tgctgcccat gcctacatca tgatgtttgt
     6061 tcatacttgc agagttggac agagtgaaat tcgttccatc tccgtaaacc agtggggctc
     6121 tcaattgggt ctgagtgttt tgagcaagct gagccagtta tactgttccc tggtgtggga
     6181 aagcactgtc ctcctctctc tgtgtacccc aaacagccta ccatctgggt gtgaatttgg
     6241 ccaggcagat atgcagaaac tggttccaaa ggatgagaag gcaggtacga cccagggcgg
     6301 aaaaagatca gatggggaac aggatggagc agctggaagt atggatgctt ctacccaggg
     6361 cttattagaa ggcattgggc tagatggtga cacattggct cccatggaga cagatgaacc
     6421 tactgcttca gactctaagg gcaaatctaa aatcacacca gcaatggctg ccagaattaa
     6481 gcaaatcaag cctttgttat cagcttcctc cagattaggc cgagcacttg ctgagctatt
     6541 tggacttctt gttaaacttt gtgtgggatc tcctgtccgc cagagaagga gccatcatgc
     6601 tgccagcacc actacagcac cgacacctgc cgcgcgatca acagcctcag ctctcactaa
     6661 gctcttgact aaggggttat cttggcagcc cccaccatat acacctactc cccgattcag
     6721 gctgacattc ttcatctgtt cagttggttt cacatcccca atgctgtttg atgagaggaa
     6781 gtatccctac cacctcatgc tgcaaaaatt tctctgctcc ggaggccaca atgctctttt
     6841 tgaaactttc aactgggctc tgtccatggg aggtaaagtt cctgtttctg agggattgga
     6901 acactcagac ttgcctgatg gcacaggaga attcctagat gcctggctta tgctggtgga
     6961 gaagatggtg aatcccacca cggtgcttga atctccacat tcgctgcctg ccaaattgcc
     7021 tggaggtgtc cagaactttc cccagttcag tgcactgcgc ttccttgtgg taactcagaa
     7081 agcagccttt acttgcatca aaaacttatg gaaccggaaa cccctgaagg tatatggtgg
     7141 acgaatggct gaatcgatgc tggccattct atgccacatc ctccgaggag aacctgtgat
     7201 tcgagagaga ctaagcaagg agaaggaggg gtctcgagga gaagaggata cagggcaaga
     7261 ggaaggtggc tcccgccggg aacctcaagt caaccagcaa caactgcaac agctcatgga
     7321 catgggcttc acaagggaac atgcaatgga ggcactgttg aacaccagca ccatggagca
     7381 ggccacagag taccttttaa cccaccctcc tccaatcatg ggaggagttg ttcgggatct
     7441 cagcatgtct gaagaggacc agatgatgag agcaattgct atgtctctgg gacaggatat
     7501 tccaatggat caaagggcag agtcacctga ggaagttgct tgccggaagg aggaagagga
     7561 acggaaagct cgggaaaagc aggaggagga agaggctaaa tgtctagaga agttccagga
     7621 tgctgacccg ttggaacaag atgagctcca cactttcaca gatactatgt tgccaggctg
     7681 cttccacctt cttgatgagc tgccagacac agtataccgt gtgtgtgacc tgatcatgac
     7741 agcaatcaaa cgtaatggag cagattatcg tgacatgatt ctgaagcaag tagtcaatca
     7801 ggtgtgggaa gctgctgatg tattgatcaa agctgctctt cccctgacaa caagtgacac
     7861 aaaaaccgtg tcagagtgga taagtcagat ggccacactg ccccaggcct ccaatttggc
     7921 tactagaatc ttgcttttaa cgctactttt tgaggagttg aagctacctt gtgcttgggt
     7981 ggttgaatca agtggcatcc ttaatgtcct aatcaaactc ttggaagtgg ttcagccctg
     8041 cctccaggca gccaaggagc agaaggaagt ccagacccca aagtggatca caccagtgtt
     8101 gctcctgatt gatttctatg aaaagacagc catctcctca aaaaggagag cccagatgac
     8161 taagtacctg caatccaaca gcaacaactg gcgctggttt gatgatcgct ctgggcgttg
     8221 gtgtagttac agtgcaagca acaatagcac tattgattct gcctggaaat ctggagagac
     8281 aagcgtgcga ttcactgcag gccgaagaag atacacggtc caattcacta caatggtgca
     8341 ggttaatgag gaaacaggga accgacgccc tgtgatgctg actctcctca gggtacctcg
     8401 gctgaataaa aattcaaaaa acagcaatgg acaggaacta gagaagacgc tggaagaaag
     8461 caaagaaatg gatatcaaac gtaaagaaaa taaaggcaat gatacccctt tggccctaga
     8521 gagtacaaac actgaaaagg agacaagcct ggaggaaaca aaaatcgggg agatcctgat
     8581 ccagggcttg acagaagata tggtgactgt tttaatccgg gcctgcgtga gcatgctggg
     8641 agtccctgtg gacccagata ctttgcatgc cacccttcgt ctctgtctga ggctcacccg
     8701 ggaccacaaa tatgccatga tgtttgcaga actgaagagt acccgcatga tcttgaattt
     8761 gacccagagc tcaggcttca atgggtttac tcccctggtc acccttctct taagacacat
     8821 cattgaggac ccctgtaccc ttcgtcatac catggaaaag gttgttcgct cagcagctac
     8881 aagtggagct ggtagcacta cctctggtgt tgtgtctggc agcctcggct ctcgggagat
     8941 caactacatc cttcgtgtcc ttgggccagc cgcatgccgc aatccagaca tattcacaga
     9001 agtggccaac tgctgtatcc gcatcgccct tcctgcccct cgaggctcag gaactgcttc
     9061 agatgatgaa tttgagaatc ttagaattaa aggccctaat gctgtacagc tggtgaagac
     9121 cacccctttg aagccctcac ctctgcctgt catccctgat actatcaagg aagtgatcta
     9181 tgatatgctg aatgctctgg ctgcatacca tgctccagag gaagccgata aatctgatcc
     9241 taaacctggg gttatgaccc aagaggttgg ccagctcctg caagacatgg gtgatgatgt
     9301 ataccagcag taccggtcac ttacgcgtca gagcagtgac tttgatacgc agtcaggttt
     9361 ttccattaat agtcaggtct ttgctgcaga tggtgcctcc actgagactt ccgcatctgg
     9421 gacctcccaa ggagaggctt caactccaga ggagtctcga gatgggaaga aagataaaga
     9481 aggggaccgg gcctctgagg aaggcaaaca gaaaggcaag ggcagcaaac ctttaatgcc
     9541 tacctccact atccttcgtc ttctggcaga gttggtgagg tcctatgttg gtattgctac
     9601 cctgattgcc aactacagct acactgtggg ccagtctgaa ctgatcaaag aggactgcag
     9661 tgtgctagct tttgttctgg accacctgct cccacatacc cagaatgcag aagacaagga
     9721 cacccctgcc ttggcccgcc tgttcctcgc aagcctggct gctgcaggga gtggcacaga
     9781 tgcccaggtg gccctagtga atgaagtaaa agcagccctt ggacgggcac tggctatggc
     9841 tgagagtaca gagaaacatg ccaggcttca ggcagtgatg tgtatcatca gtactatcat
     9901 ggagtcctgc ccctccacct ccagcttcta cagcagtgcc acagcgaaga cccagcacaa
     9961 tggcatgaac aacatcattc ggcttttcct gaagaaggga ctggttaatg acctggccag
    10021 agtacctcac agcttagacc tgtccagtcc caacatggcc aacacagtca atgctgctct
    10081 gaagcctttg gaaacacttt cccggattgt gaaccagccc agtagccttt ttggcagcaa
    10141 gagtgcttct agcaagaaca agtctgagca ggatgcccaa ggagcctctc aagattccag
    10201 tagcaaccag caggacccag gcgagcctgg ggaagcagaa gtgcaggagg aggatcatga
    10261 tgtcactcag acagaggtgg cagatgggga tatcatggat ggggaggctg aaaccgactc
    10321 agtggtgatt gctgggcagc ctgaggtgct cagttcacaa gagatgcagg ttgagaatga
    10381 gctggaggac ctgatagatg agttgcttga gagggatggc ggatctggga acagtacaat
    10441 tatagtgagc agaagtggag aggatgaatc acaagaggac gtgctgatgg atgaagctcc
    10501 ttccaacctc agccaagctt ccaccttgca ggccaaccga gaagattcca tgaatatcct
    10561 ggaccctgag gatgaggagg agcacactca ggaagaggac agcagtggca gtaacgagga
    10621 tgaggatgat agtcaggatg aagaggagga ggaggaggaa gatgaggaag atgatcagga
    10681 ggatgatgaa ggtgaagagg gagatgaaga cgatgacgac gatggctctg agatggaatt
    10741 ggatgaggat tatcctgata tgaacgcttc tcccttggtc cgatttgagc gctttgaccg
    10801 ggaggatgat ctcatcattg agtttgacaa catgttctcc agtgctacag acatcccccc
    10861 atccccagga aatatcccta ccacccatcc actgatggtg cgccatgcag accacagttc
    10921 tctgacactg ggcagtggct cttcaacaac tcgtctcacc cagggcatcg ggcgcagtca
    10981 gaggacccta aggcagctga cggccaatac tggccacacc attcatgttc actaccctgg
    11041 gaatcgccag cccaaccctc ctcttatact gcagaggttg cttggtccct cagctgctgc
    11101 tgacatcctt cagctgagca gcagccttcc cctacaaagc cggggtcggg cccgcctcct
    11161 ggtaggcaac gatgacgtcc acatcatcgc ccgttctgat gatgagctgc tggatgactt
    11221 tttccatgat cagagcacag ctaccagcca agcaggaacc ctgtccagca tccccacagc
    11281 cctgacccgc tggacagaag aatgcaaagt tctcgatgct gagagcatgc atgactgtgt
    11341 ttcagtggtt aaagtgtcca ttgtcaatca cctggaattc ctgagggatg aggagctgga
    11401 agaaaggcga gagaagcgca ggaaacaact ggctgaggaa gaaacaaaga taactgataa
    11461 aggcaaagaa gataaggaga acagggatca gagtgcacag tgtactgcat ctaagtcaaa
    11521 tgactccact gaacagaatc tctcagatgg gacgcctatg cctgacagct acccaacaac
    11581 cccatcttca actgatgcag ctacatctga gtccaaggag acccttggca ctctgcaatc
    11641 ctcacaacag caaccaacac tcccaacccc accagctttg ggagaggttc ctcaggagct
    11701 gcagtctcca gctggagaag ggggcagctc tacacagcta ttgatgcctg tagagccaga
    11761 ggaattgggt cccacaaggc caagtgggga agcagaaaca actcagatgg agttatcccc
    11821 agctcccact ataacctcac tttccccaga gagagctgag gattctgatg cactgacggc
    11881 tgtcagcagt cagctagaag gctctcctat ggatacaagc agcctggctt cctgtacctt
    11941 agaggaggct gtgggtgaca cttcagcagc tggcagttct gagcagccca gagcaggcag
    12001 ctccactcct ggggatgccc caccagctgt ggcggaagtg caaggcagga gtgatgggtc
    12061 aggggaatct gcccagccac ctgaggacag ctccccacct gcatcctctg agagctcttc
    12121 caccagagat tctgccgtgg ccatttctgg cgccgattcc cgaggaatcc tagaagagcc
    12181 gttgccttca acaagcagtg aagaagaaga tccccttgcg ggtatcagtc tccctgaagg
    12241 tgtggacccc tcttttctgg ctgccctgcc tgatgacatc cgtcgggaag ttctacagaa
    12301 ccagctaggc attcgtccac caacccggac tgccccctcc acaaatagct cagcgcctgc
    12361 agtggtgggg aatcctggtg tgactgaggt gagtcccgag tttcttgcgg cactgccacc
    12421 cgcgatccaa gaggaagtac tggcacagca gagagctgag cagcagcgac gagaactagc
    12481 acagaatgcc agctcagaca cccctatgga ccctgtgacc ttcatccaga ctctgccctc
    12541 agacctgcgc cgtagtgtcc tagaggatat ggaggacagt gtgttagctg tgatgccacc
    12601 tgacattgca gctgaggctc aagccctgag acgagagcaa gaagcccggc agcgacagct
    12661 catgcatgag cgtctgtttg ggcacagtag cacctccgca ctctctgcta ttctccgaag
    12721 cccggctttc accagtcgct taagtggcaa ccgtggggtc cagtatactc gccttgctgt
    12781 gcagagaggt ggcaccttcc agatgggggg tagcagcagc cataacaggc cttctggcag
    12841 taatgtagat actctcctcc gcctccgagg acggctcctt ctggaccacg aagccctttc
    12901 ttgtctcttg gtcctacttt ttgtggatga gccaaagctc aatactagcc gtctacaccg
    12961 agtactgaga aatctctgct accatgccca gacccgccac tgggtcatcc gcagtctgct
    13021 ctccatcttg cagcgcagca gtgagagtga gctatgcatt gaaacaccca aactcactac
    13081 aagtgaggaa aagggcaaaa agtcgagcaa gagctgtggg tcaagtagcc atgagaaccg
    13141 tcccctggac ctgctacaca agatggagtc aaagagctcc aaccagcttt cctggctctc
    13201 agtatccatg gatgcagccc taggctgcag gactaatata tttcagatcc agcgttcagg
    13261 ggggcgtaaa cataccgaga agcatgcaag cggtggctcc accgtccaca tccatcccca
    13321 agctgctcct gttgtctgca gacacgtttt ggatacactc attcaattgg ccaaggtatt
    13381 tcccagccac ttcacacagc agcggaccaa agaaacaaac tgtgagagtg atcgggaaag
    13441 gggcaataag gcctgtagcc catgctcctc acagtcctcc agcagtggca tttgcacaga
    13501 cttctgggac ttattggtaa aactggacaa catgaatgtc agccggaaag gcaagaactc
    13561 cgtgaagtca gtgccagtga gcgctggcgg tgagggggaa acctctccat acagcctcga
    13621 ggcctctcca ctggggcagc tcatgaacat gttgtcacac ccagtcatcc gccggagctc
    13681 tctcttaact gagaaactcc tcagactcct ttctctcatc tcaattgctc tcccagaaaa
    13741 caaggtgtca gaagcacagg ctaattctgg cagcggtgct tcctccacca ccactgccac
    13801 ctcaaccaca tctaccacca ccaccactgc cgcctccacc acgcccacac cccctactgc
    13861 acccacccct gtcacttctg ctccagccct ggttgctgcc acggctattt ccaccattgt
    13921 cgtagctgct tcgaccacag tgactacccc cacgactgct accactactg tttcaatttc
    13981 tcccactact aagggcagca aatctccagc gaaggtgagt gatgggggca gcagcagtac
    14041 agactttaag atggtgtcct ctggcctcac tgaaaaccag ctacagctct ctgtagaggt
    14101 gttgacatcc cactcttgtt ctgaggaagg cttagaggat gcagccaacg tactactgca
    14161 gctctcccgg ggggactctg ggacccggga cactgttctc aagctgctac tgaatggagc
    14221 ccgccatctg ggttataccc tttgtaaaca aataggtacc ctgctggccg agctgcggga
    14281 atacaacctc gagcagcagc ggcgagccca atgtgaaacc ctctctcctg atggcctgcc
    14341 tgaggagcag ccacagacca ccaagctgaa gggcaaaatg cagagcaggt ttgacatggc
    14401 tgagaatgtg gtaattgtgg catctcagaa gcgacctttg ggtggccggg agctccagct
    14461 gccttctatg tccatgttga catccaagac atctacccag aagttcttct tgagggtact
    14521 acaggtcatc atccagctcc gggacgacac gcgccgggct aacaagaaag ccaagcagac
    14581 aggcaggcta ggttcctccg gtttaggctc agctagcagc atccaggcag ctgttcggca
    14641 gctggaggct gaggctgatg ccattataca aatggtacgt gagggtcaaa gggcgcggag
    14701 acagcaacaa gcagcaacgt cggagtctag ccagtcagag gcgtctgtcc ggagggagga
    14761 atcacccatg gatgtggacc agccatctcc cagtgctcaa gatactcaat ccattgcctc
    14821 cgatggaacc ccacaggggg agaaggaaaa ggaagaaaga ccacctgagt tacccctgct
    14881 cagcgagcag ctgagtttgg acgagctgtg ggacatgctt ggggagtgtc taaaggaact
    14941 agaggaatcc catgaccagc atgcggtgct agtgctacag cctgctgtcg aggccttctt
    15001 tctggtccat gccacagagc gggagagcaa gcctcctgtc cgagacaccc gtgagagcca
    15061 gctggcacac atcaaggacg agcctcctcc actctcccct gcccccttaa ccccagccac
    15121 gccttcctcc cttgacccat tcttctcccg ggagccctca tctatgcaca tctcctcaag
    15181 cctgccccct gacacacaga agttccttcg ctttgcagag actgaccgca ctgtgttaaa
    15241 ccagatccta cggcagtcca cgacccacct tgctgatggg ccttttgctg tcctggtaga
    15301 ctacattcgt gtcctcgact ttgatgtcaa gcgcaaatat ttccgccaag agctggagcg
    15361 tttagatgag gggctccgga aagaagacat ggctgtgcat gtccgtcgtg accatgtgtt
    15421 tgaagactcc tatcgtgagc tgcatcgcaa atcccccgaa gaaatgaaga atcgattgta
    15481 tatagtattt gaaggagaag aagggcagga tgctggcggg ctcctgcggg agtggtatat
    15541 gatcatctct cgagagatgt ttaaccctat gtatgccttg ttccgtacct cacctggtga
    15601 tcgagtcacc tacaccatca atccatcttc ccactgcaac cccaaccatc tgtcctattt
    15661 taaatttgtc ggacgcattg tggccaaagc tgtatatgac aaccgtcttc tggagtgcta
    15721 ctttactcga tccttttaca aacacatctt gggcaagtca gtcagatata cagatatgga
    15781 gagtgaagat taccacttct accaaggtct ggtttatctg ctggaaaatg atgtctccac
    15841 actaggctat gacctcacct tcagcactga ggtccaagag tttggagttt gtgaagttcg
    15901 tgacctcaaa cccaatgggg ccaacatctt ggtaacagag gagaataaga aggagtatgt
    15961 acacctggta tgccagatga gaatgacagg agccatccgc aagcagttgg cggctttctt
    16021 agaaggcttc tatgagatca ttccaaagcg cctcatttcc atcttcactg agcaggagtt
    16081 agagctgctt atatcaggac tgcccaccat tgacatcgat gatctgaaat ccaacactga
    16141 ataccacaag taccagtcca actctattca gatccagtgg ttctggagag cattgcgttc
    16201 tttcgatcaa gctgaccgtg ccaagttcct ccagtttgtc acgggtactt ccaaggtacc
    16261 cctgcaaggc tttgctgccc tcgaaggcat gaatggcatt cagaagtttc agatccatcg
    16321 agatgacagg tccacagatc gcctgccttc agctcacaca tgttttaatc agctggatct
    16381 gcctgcctat gagagctttg agaagctccg ccacatgcta ctgttggcta tccaggagtg
    16441 ctctgaaggc tttgggctgg cctaataagc ggccgctgat gataatggcc tgtggccgta
    16501 atggcctgtg gccgtaaaat caacctctgg attacaaaat ttgtgaaaga ttgactggta
    16561 ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc
    16621 atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt
    16681 ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg
    16741 ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt
    16801 tcgctttccc cctccctatt gccacggcgg aactcagtgc cgcctgcctt gcccgctgct
    16861 ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg aaatcatcgt
    16921 cctttccttg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct
    16981 acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc
    17041 ggcctcttcc gcggcttcga gatctgcctc gactgtgcct tctagttgcc agccatctgt
    17101 tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc
    17161 ctaataaaat gaggaaattg catcaaattg tctgagtagg tgtcattcta ttctgggggg
    17221 tggggtgggg caggacagca agggggagga ttgggcagac aatagcaggc atgctgggga
    17281 ctcgagttaa gggcgaatcg ctgggacgcc cgaacggtgg ctactcgttg acataagcct
    17341 gttcggttcg taaactgtaa tgcaagtagc gtatgcgctc acgcaactgg tccagaacct
    17401 tgaccgaacg cagcggtggt aacggcgcag tggcggtttt catggcttgt tatgactgtt
    17461 tttttgtaca gtctatgcct cgggcatcca agcagcaagc gcgttacgcc gtgggtcgat
    17521 gtttgatgtt atggagcagc aacgatgtta cgcagcagca acgatgttac gcagcagggc
    17581 agtcgcccta aaacaaagtt aggtggctca agtatgggca tcattcgtac atgtaggctc
    17641 ggccctgacc aagtcaaatc catgcgggct gctcttgatc ttttcggtcg tgagttcggt
    17701 gatgtagcca cctactccca acatcagccg gactccgatt acctcgggaa cttgctccgt
    17761 agtaagacat ttattgcgct tgctgccttc gaccaagaag cggttgttgg cgctctcgcg
    17821 gcttacgttc tgcccaagtt tgagcagccg cgtagtgaga tctatatcta tgatctcgca
    17881 gtctccggcg agcaccggag gcagggcatt gccaccgcgc tcatcaatct cctcaagcat
    17941 gaggccaacg cgcttggtgc ttatgtgatc tacgtgcaag cagattacgg tgacgatccc
    18001 gcagtggctc tctatacaaa gttgggcata cgggaagaag tgatgcactt tgatatcgac
    18061 ccaagtaccg ccacctaaca attcgttcaa gccgagatcg gcttcccggc cgcggagttg
    18121 ttcggtaaat tgtcacaacg ccgc
//
