LOCUS       sequence-37007-e         940 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3rd gen lentiviral vector for expression of Reverse Tetracycline 
            transactivator 3G (rtTA3G)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 940)
  TITLE     pLenti CMV rtTA3G Blast
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 940)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLenti CMV rtTA3G Blast (R980-M38-658)
FEATURES             Location/Qualifiers
     source          1..940
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    101..125
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             128..874
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     protein_bind    complement(873..897)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 cgtgggcttt agcgactgtt agtgaaccgt cagatcgcct ggagacgcca tccacgctgt
       61 tttgacctcc atagaagaca ccgggaccga tccagcctcc gcaactttgt acaaaaaagt
      121 tggcaccatg agccggctgg acaagagcaa agtgatcaac agcgccctgg aactgctgaa
      181 cggcgtgggc atcgagggcc tgaccacccg gaagctggcc cagaaactgg gcgtggaaca
      241 gcccaccctg tactggcacg tgaagaacaa gcgggccctg ctggacgccc tgcccatcga
      301 gatgctggac cggcaccaca cccacagctg ccctctggaa ggcgagagct ggcaggactt
      361 cctgcggaac aacgccaaga gctacagatg cgccctgctg agccaccggg acggcgccaa
      421 agtgcacctg ggcaccagac ccaccgagaa gcagtacgag acactggaaa accagctggc
      481 cttcctgtgc cagcagggct tcagcctgga aaacgccctg tacgccctga gcgccgtggg
      541 ccacttcacc ctgggctgcg tgctggaaga acaggaacac caggtcgcca aagaggaaag
      601 agagacaccc accaccgaca gcatgccccc cctgctgaag caggccatcg agctgttcga
      661 cagacagggc gccgagcccg ccttcctgtt cggcctggaa ctgatcatct gcggcctcga
      721 gaagcagctg aagtgcgaga gcggcggacc caccgacgcc ctggacgact tcgacctgga
      781 catgctgccc gccgatgccc tggatgattt tgatctggat atgctccctg ctgacgctct
      841 cgatgacttc gatctcgata tgctgccagg ctaatcaact ttcttgtaca aagttgtgat
      901 gtggcggccg ctcgacaatc aacctctgga ttacaaaatt
//
