LOCUS       sequence-37008-e         907 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 907)
  AUTHORS   Waldner C, Rempel O, Schutte F, Yanik M, Solomentsew N, Ryffel GU
  TITLE     Double conditional human embryonic kidney cell line based on FLP and
            PhiC31 mediated transgene integration.
  JOURNAL   BMC Res Notes. 2011 Oct 18;4:420.
  PUBMED    22008483
REFERENCE   2  (bases 1 to 907)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Res 
            Notes."; date: "2011-10-18"; volume: "4"; pages: "420"
COMMENT     Sequence Label: FR_DsRed2
FEATURES             Location/Qualifiers
     source          1..907
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      156..165
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(287..311)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    321..339
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    342..360
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        complement(362..565)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(391..411)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        566..869
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gnaggagggc gcctggtact tcagcttcac ggtgttgtgg ccctcgtagg ggcggccctc
       61 gccctcgccc tcgatctcga actcgtggcc gttcacggtg ccctccatgc gcaccttgaa
      121 gcgcatgaac tcggtgatga cgttctcgga ggaggccatg gtggcgaccg gtacccgggg
      181 atccgagctc ggtaccaagc ttaagtttaa acgctagagt ccggaggctg gatcggtccc
      241 ggtgtcttct atggaggtca aaacagcgtg gatggcgtct ccaggcgatc tgacggttca
      301 ctaaacgagc tcgtcgacga tctctatcac tgatagggag atctctatca ctgataggga
      361 gagctctgct tatatagacc tcccaccgta cacgcctacc gcccatttgc gtcaatgggg
      421 cggagttgtt acgacatttt ggaaagtccc gttgattttg gtgccaaaac aaactcccat
      481 tgacgtcaat ggggtggaga cttggaaatc cccgtgagtc aaaccgctat ccacgcccat
      541 tgatgtactg ccaaaaccgc atcaccatgg taatagcgat gactaatacg tagatgtact
      601 gccaagtagg aaagtcccat aaggtcatgt actgggcata atgccaggcg ggccatttac
      661 cgtcattgac gtcaataggg ggcgtacttg gcatatgata cacttgatgt actgccaagt
      721 gggcagttta ccgtaaatac tccacccatt gacgtcaatg gaaagtccct attggcgtta
      781 ctatgggaac atacgtcatt attgacgtca atgggcgggg gtcgttgggc ggtcagccag
      841 gcgggccatt taccgtaagt tatgtaacgc ggaactccat atatgggcta tgaactaatg
      901 accccgt
//
