LOCUS       sequence-370081-        9309 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Hippo - TEAD1 gene reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9309)
  AUTHORS   O'Connell DJ, Kolde R, Sooknah M, Graham DB, Sundberg TB, Latorre 
            IJ, Mikkelsen TS, Xavier RJ
  TITLE     Simultaneous Pathway Activity Inference and Gene Expression Analysis
            Using RNA Sequencing.
  JOURNAL   Cell Syst. 2016 May 25;2(5):323-34. doi: 10.1016/j.cels.2016.04.011.
            Epub 2016 May 19.
  PUBMED    27211859
REFERENCE   2  (bases 1 to 9309)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cels.2016.04.011"; journalName: "Cell Syst"; date: 
            "2016-05-25- 25"; volume: "2"; issue: "5"; pages: "323-34"
COMMENT     Sequence Label: pLminP_Luc2P_RE31
FEATURES             Location/Qualifiers
     source          1..9309
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        3..229
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             230..410
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    457..582
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1075..1308
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1493..1537
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1804..1921
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2140..2171
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     regulatory      2198..2207
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2204..3853
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     CDS             3857..3976
                     /codon_start=1
                     /product="PEST degradation sequence from mouse ornithine 
                     decarboxylase"
                     /label=hPEST
                     /note="human codon-optimized"
                     /translation="SHGFPPEVEEQAAGTLPMSCAQESGMDRHPAACASARINV"
     misc_feature    4069..4657
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4122..4142)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4540..4551)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        4672..4988
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4839..4974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             join(5039..5041,5042..5044,5045..5758)
                     /codon_start=1
                     /product="Emerald GFP"
                     /label=EmGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5084..5105)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5345..5364)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5692..5713
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     LTR             5830..6063
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6135..6256
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6172..6191)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6226..6245
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6296..6431
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6451..6470)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6452..6470)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6480..6497)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6480..6496)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6489..6511)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6638..7093
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6725..6744)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6935..6956
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7119..7223
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7224..7292,7293..8084)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7442..7461)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8255..8843
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8744..8763
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8997..9014
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9131..9152
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9167..9172,9173..9190,9191..9197)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9205..9221
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9210..9232
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9229..9245
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9229..9245
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9264..9284
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9266..9284
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
       61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
      181 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
      241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      361 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
      421 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
      481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
      541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
      601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
      661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
      721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
      781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
      841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
      901 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
      961 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1021 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1081 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1141 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1201 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1261 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1321 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1381 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatcgc accagcgtgt ggatcgacac acattccaca gctggacaca cattccacag
     1981 ctggacacac attccacagc tggacacaca ttccacagct ggacacacat tccacagctg
     2041 gacacacatt ccacagctgg acacacattc cacagctgga cacacattcc acagctggat
     2101 ccgagaacag atctggcctc ggcggccaag cttagacact agagggtata taatggaagc
     2161 tcgacttcca gcttggcaat ccggtactgt tggtaaagcc accatggaag atgccaaaaa
     2221 cattaagaag ggcccagcgc cattctaccc actcgaagac gggaccgccg gcgagcagct
     2281 gcacaaagcc atgaagcgct acgccctggt gcccggcacc atcgccttta ccgacgcaca
     2341 tatcgaggtg gacattacct acgccgagta cttcgagatg agcgttcggc tggcagaagc
     2401 tatgaagcgc tatgggctga atacaaacca tcggatcgtg gtgtgcagcg agaatagctt
     2461 gcagttcttc atgcccgtgt tgggtgccct gttcatcggt gtggctgtgg ccccagctaa
     2521 cgacatctac aacgagcgcg agctgctgaa cagcatgggc atcagccagc ccaccgtcgt
     2581 attcgtgagc aagaaagggc tgcaaaagat cctcaacgtg caaaagaagc taccgatcat
     2641 acaaaagatc atcatcatgg atagcaagac cgactaccag ggcttccaaa gcatgtacac
     2701 cttcgtgact tcccatttgc cacccggctt caacgagtac gacttcgtgc ccgagagctt
     2761 cgaccgggac aaaaccatcg ccctgatcat gaacagtagt ggcagtaccg gattgcccaa
     2821 gggcgtagcc ctaccgcacc gcaccgcttg tgtccgattc agtcatgccc gcgaccccat
     2881 cttcggcaac cagatcatcc ccgacaccgc tatcctcagc gtggtgccat ttcaccacgg
     2941 cttcggcatg ttcaccacgc tgggctactt gatctgcggc tttcgggtcg tgctcatgta
     3001 ccgcttcgag gaggagctat tcttgcgcag cttgcaagac tataagattc aatctgccct
     3061 gctggtgccc acactattta gcttcttcgc taagagcact ctcatcgaca agtacgacct
     3121 aagcaacttg cacgagatcg ccagcggcgg ggcgccgctc agcaaggagg taggtgaggc
     3181 cgtggccaaa cgcttccacc taccaggcat ccgccagggc tacggcctga cagaaacaac
     3241 cagcgccatt ctgatcaccc ccgaagggga cgacaagcct ggcgcagtag gcaaggtggt
     3301 gcccttcttc gaggctaagg tggtggactt ggacaccggt aagacactgg gtgtgaacca
     3361 gcgcggcgag ctgtgcgtcc gtggccccat gatcatgagc ggctacgtta acaaccccga
     3421 ggctacaaac gctctcatcg acaaggacgg ctggctgcac agcggcgaca tcgcctactg
     3481 ggacgaggac gagcacttct tcatcgtgga ccggctgaag agcctgatca aatacaaggg
     3541 ctaccaggta gccccagccg aactggagag catcctgctg caacacccca acatcttcga
     3601 cgccggggtc gccggcctgc ccgacgacga tgccggcgag ctgcccgccg cagtcgtcgt
     3661 gctggaacac ggtaaaacca tgaccgagaa ggagatcgtg gactatgtgg ccagccaggt
     3721 tacaaccgcc aagaagctgc gcggtggtgt tgtgttcgtg gacgaggtgc ctaaaggact
     3781 gaccggcaag ttggacgccc gcaagatccg cgagattctc attaaggcca agaagggcgg
     3841 caagatcgcc gtgaattctc acggcttccc tcccgaggtg gaggagcagg ccgccggcac
     3901 cctgcccatg agctgcgccc aggagagcgg catggataga caccctgctg cttgcgccag
     3961 cgccaggatc aacgtctaag gccgcgactc tagaacgcgt cactgcggct tataaatgga
     4021 tagggcttga ctacagacat cagcgcgtac cggttagtaa tgatcgacaa tcaacctctg
     4081 gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta
     4141 tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt
     4201 ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc
     4261 aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt
     4321 gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg
     4381 gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac
     4441 aattccgtgg tgttgtcggg gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc
     4501 acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac
     4561 cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct
     4621 cagacgagtc ggatctccct ttgggccgcc tccccgcctg gcgatggtac cggtgtggaa
     4681 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
     4741 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
     4801 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     4861 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     4921 ccgcctctgc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     4981 tttgcaaaaa gctcccggga gcttgtatat ccattttcgg atctgatcag cacctagcat
     5041 ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg
     5101 cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg
     5161 caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct
     5221 cgtgaccacc ttcacctacg gcgtgcagtg cttcgcccgc taccccgacc acatgaagca
     5281 gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt
     5341 caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt
     5401 gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa
     5461 gctggagtac aactacaaca gccacaaggt ctatatcacc gccgacaagc agaagaacgg
     5521 catcaaggtg aacttcaaga cccgccacaa catcgaggac ggcagcgtgc agctcgccga
     5581 ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta
     5641 cctgagcacc cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct
     5701 gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagtaagg
     5761 tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa
     5821 aggggggact ggaagggcta attcactccc aacgaagaca agatctgctt tttgcttgta
     5881 ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc
     5941 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt
     6001 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta
     6061 gcagtagtag ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat
     6121 cagagagtga gaggaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
     6181 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
     6241 tcatcaatgt atcttatcat gtctggctct agctatcccg cccctaactc cgcccatccc
     6301 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat
     6361 ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt
     6421 ttttggaggc ctagggacgt acccaattcg ccctatagtg agtcgtatta cgcgcgctca
     6481 ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc
     6541 cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc
     6601 ccttcccaac agttgcgcag cctgaatggc gaatgggacg cgccctgtag cggcgcatta
     6661 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
     6721 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
     6781 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
     6841 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
     6901 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
     6961 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc
     7021 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta
     7081 acgcttacaa tttaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
     7141 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
     7201 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct
     7261 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag
     7321 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta
     7381 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc
     7441 tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca
     7501 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg
     7561 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg
     7621 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca
     7681 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa
     7741 acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa
     7801 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata
     7861 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat
     7921 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc
     7981 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata
     8041 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt
     8101 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
     8161 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     8221 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     8281 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     8341 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     8401 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     8461 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     8521 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     8581 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     8641 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     8701 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     8761 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     8821 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     8881 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
     8941 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
     9001 agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt
     9061 ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc
     9121 gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc
     9181 ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct
     9241 atgaccatga ttacgccaag cgcgcaatta accctcacta aagggaacaa aagctggagc
     9301 tgcaagctt
//
