LOCUS       sequence-370114-        9272 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses MUC2 D1D2D'D3 assembly with cleavable His tag in mammalian
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9272)
  AUTHORS   Reznik N, Gallo AD, Rush KW, Javitt G, Fridmann-Sirkis Y, Ilani T, 
            Nairner NA, Fishilevich S, Gokhman D, Chacon KN, Franz KJ, Fass D
  TITLE     Intestinal mucin is a chaperone of multivalent copper.
  JOURNAL   Cell. 2022 Oct 27;185(22):4206-4215.e11. doi: 
            10.1016/j.cell.2022.09.021. Epub 2022 Oct 6.
  PUBMED    36206754
REFERENCE   2  (bases 1 to 9272)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.09.021"; journalName: "Cell"; date: 
            "2022-10-27- 27"; volume: "185"; issue: "22"; pages: "4206-4215.e11"
COMMENT     Sequence Label: pcDNA3.1-MUC2 D1D2D'D3 with cleavable His tag
FEATURES             Location/Qualifiers
     source          1..9272
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(78..97)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        269..648
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        649..852
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     803..823
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     897..916
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        897..915
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1068..1085
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             1086..1106
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     primer_bind     complement(4897..4914)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4903..5127
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5173..5601
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5260..5279)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5470..5491
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5610..5630)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5615..5944
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5795..5930
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5857..5876
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6011..6805
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6065..6084)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6675..6694
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6979..7100
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7016..7035)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7070..7089
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7149..7165)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7149..7165)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7162..7184)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7173..7189
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7197..7203,7204..7221,7222..7227))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7242..7263
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7380..7397)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7551..8139)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7631..7650)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8310..9101,9102..9170))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8933..8952
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(join(9171..9272,1..3))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gcgcacattt ccccgaaaag tgccacctga cgtcgacgga tcgggagatc tcccgatccc
       61 ctatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatctgctc
      121 cctgcttgtg tgttggaggt cgctgagtag tgcgcgagca aaatttaagc tacaacaagg
      181 caaggcttga ccgacaattg catgaagaat ctgcttaggg ttaggcgttt tgcgctgctt
      241 cgcgatgtac gggccagata tacgcgttga cattgattat tgactagtta ttaatagtaa
      301 tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg
      361 gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg
      421 tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta
      481 cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt
      541 gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac
      601 tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt
      661 tggcagtaca tcaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac
      721 cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt
      781 cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat
      841 ataagcagag ctctctggct aactagagaa cccactgctt actggcttat cgaaattaat
      901 acgactcact atagggagac ccaagctggc tagcgtttaa acttaagctt ggtaccgagc
      961 tcggatccat gaggaggtgc aacagcggct ccgggccgcc gccgtcgctg ctgctgctgt
     1021 tgctgtggct gctcgcggtt cccggcgcta acgcggcccc gcagggtcat catcatcacc
     1081 atcacgagaa tctctatttc cagggatcgg agctccagac agagggcaga acccgaaacc
     1141 acggccacaa cgtctgcagc acctggggca acttccacta caagaccttc gacggggacg
     1201 tcttccgctt ccccggcctc tgcgactaca acttcgcctc cgactgccga ggctcctaca
     1261 aggaatttgc tgtgcacctg aagcggggtc cgggccaggc tgaggccccc gccggggtgg
     1321 agtccatcct gctgaccatc aaggatgaca ccatctacct cacccgccac ctggctgtgc
     1381 ttaacggggc cgtggtcagc accccgcact acagccccgg gctgctcatt gagaagagcg
     1441 atgcctacac caaagtctac tcccgcgccg gcctcaccct catgtggaac cgggaggatg
     1501 cactcatgct ggagctggac actaagttcc ggaaccacac ctgtggcctc tgcggggact
     1561 acaacggcct gcagagctat tcagaattcc tctctgacgg cgtgctcttc agtcccctgg
     1621 agtttgggaa catgcagaag atcaaccaac ccgatgtggt gtgtgaggat cccgaggagg
     1681 aggtggcccc cgcatcctgc tccgagcacc gcgccgagtg tgagaggctg ctgaccgccg
     1741 aggccttcgc ggactgtcag gacctggtgc cgctggagcc gtatctgcgc gcctgccagc
     1801 aggaccgctg ccggtgcccg ggcggtgaca cctgcgtctg cagcaccgtg gccgagttct
     1861 cccgccagtg ctcccacgcc ggcggccggc ccgggaactg gaggaccgcc acgctctgcc
     1921 ccaagacctg ccccgggaac ctggtgtacc tggagagcgg ctcgccctgc atggacacct
     1981 gctcacacct ggaggtgagc agcctgtgcg aggagcaccg catggacggc tgtttctgcc
     2041 cagaaggcac cgtatatgac gacatcgggg acagtggctg cgttcctgtg agccagtgcc
     2101 actgcaggct gcacggacac ctgtacacac cgggccagga gatcaccaat gactgcgagc
     2161 agtgtgtctg taacgctggc cgctgggtgt gcaaagacct gccctgcccc ggcacctgtg
     2221 ccctggaagg cggctcccac atcaccacct tcgatgggaa gacgtacacc ttccacgggg
     2281 actgctacta tgtcctggcc aagggtgacc acaacgattc ctatgctctc ctgggcgagc
     2341 tggccccctg tggctccaca gacaagcaga cctgcctgaa gacggtggtg ctgctggctg
     2401 acaagaagaa gaatgtggtg gtcttcaagt ccgatggcag tgtactgctc aacgagctgc
     2461 aggtgaacct gccccacgtg accgcgagct tctctgtctt ccgcccgtct tcctaccaca
     2521 tcatggtgag catggccatt ggcgtccggc tgcaggtgca gctggcccca gtcatgcaac
     2581 tctttgtgac actggaccag gcctcccagg ggcaggtgca gggcctctgc gggaacttca
     2641 acggcctgga aggtgacgac ttcaagacgg ccagcgggct ggtggaggcc acgggggccg
     2701 gctttgccaa cacctggaag gcacagtcaa gctgccatga caagctggac tggttggacg
     2761 atccctgctc cctgaacatc gagagcgcca actacgccga gcactggtgc tccctcctga
     2821 agaagacaga gacccccttt ggcaggtgcc actcggctgt ggaccctgct gagtattaca
     2881 agaggtgcaa atatgacacg tgtaactgtc agaacaatga ggactgcctg tgcgccgccc
     2941 tgtcctccta cgcgcgcgcc tgcaccgcca agggcgtcat gctgtggggc tggcgggagc
     3001 atgtctgcaa caaggatgtg ggctcctgcc ccaactcgca ggtcttcctg tacaacctga
     3061 ccacctgcca gcagacctgc cgctccctct ccgaggccga cagccactgt ctcgagggct
     3121 ttgcgcctgt ggacggctgc ggctgccctg accacacctt cctggacgag aagggccgct
     3181 gcgtacccct ggccaagtgc tcctgttacc accgcggtct ctacctggag gcgggggatg
     3241 tggtcgtcag gcaggaagaa cgatgtgtgt gccgggatgg gaggctgcac tgtaggcaga
     3301 tccggctgat cggccagagc tgcacggccc caaagatcca catggactgc agcaacctga
     3361 ctgcactggc cacctcgaag ccccgagccc tcagctgcca gacgctggcc gccggctatt
     3421 accacacaga gtgtgtcagt ggctgtgtgt gccccgacgg gctgatggat gacggccggg
     3481 gtggctgtgt ggtggagaag gaatgccctt gcgtccataa caacgacctg tattcttccg
     3541 gcgccaagat caaggtggac tgcaatacct gcacctgcaa gagaggacgc tgggtgtgca
     3601 cccaggctgt gtgccatggc acctgctcca tttacgggag tggccactac atcacctttg
     3661 atgggaagta ctacgacttt gacggacact gctcctacgt ggctgttcag gactactgcg
     3721 gccagaactc ctcactgggc tcattcagca tcatcaccga gaacgtcccc tgtggcacta
     3781 cgggcgtcac ctgctccaag gccattaaga tcttcatggg gaggacggag ctgaagttgg
     3841 aagacaagca ccgtgtggtg atccagcgtg atgagggtca ccacgtggcc tacaccacgc
     3901 gggaggtggg ccagtacctg gtggtggagt ccagcacggg catcatcgtc atctgggaca
     3961 agaggaccac cgtgttcatc aagctggctc cctcctacaa gggcaccgtg tgtggcctgt
     4021 gtgggaactt tgaccaccgc tccaacaacg acttcaccac gcgggaccac atggtggtga
     4081 gcagcgagct ggacttcggg aacagctgga aggaggcccc cacctgccca gatgtgagca
     4141 ccaaccccga gccctgcagc ctgaacccgc accgccgctc ctgggccgag aagcagtgca
     4201 gcatcctcaa aagcagcgtg ttcagcatct gccacagcaa ggtggacccc aagcccttct
     4261 acgaggcctg tgtgcacgac tcgtgctcct gtgacacggg tggggactgt gagtgcttct
     4321 gctctgccgt ggcctcctac gcccaggagt gtaccaaaga gggggcctgc gtgttctgga
     4381 ggacgccgga cctgtgcccc atattctgcg actactacaa ccctccgcat gagtgtgagt
     4441 ggcactatga gccatgtggg aaccggagct tcgagacctg caggaccatc aacggcatcc
     4501 actccaacat ctccgtgtcc tacctggagg gctgctaccc ccggtgcccc aaggacaggc
     4561 ccatctatga ggaggatctg aagaagtgtg tcactgcaga caagtgtggc tgctatgtcg
     4621 aggacaccca ctacccacct ggagcatcgg ttcccaccga ggagacctgc aagtcctgcg
     4681 tgtgtaccaa ctcctcccaa gtcgtctgca ggccggaaga aggaaagatt cttaaccaga
     4741 cccaggatgg cgccttctgc tactgggaga tctgtggccc caacgggacg gtggagaagc
     4801 acttcaacat ctgttccatt acgtgagaat tctgcagata tccagcacag tggcggccgc
     4861 tcgagtctag agggcccgtt taaacccgct gatcagcctc gactgtgcct tctagttgcc
     4921 agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca
     4981 ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta
     5041 ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc
     5101 atgctgggga tgcggtgggc tctatggctt ctgaggcgga aagaaccagc tggggctcta
     5161 gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc
     5221 gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt
     5281 cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag
     5341 ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt
     5401 cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt
     5461 tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt
     5521 cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt
     5581 aacaaaaatt taacgcgaat taattctgtg gaatgtgtgt cagttagggt gtggaaagtc
     5641 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag
     5701 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
     5761 gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
     5821 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
     5881 ctctgcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg
     5941 caaaaagctc ccgggagctt gtatatccat tttcggatct gatcaagaga caggatgagg
     6001 atcgtttcgc atgattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga
     6061 gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt
     6121 ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct
     6181 gaatgaactg caggacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg
     6241 cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt
     6301 gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc
     6361 tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc
     6421 gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga
     6481 tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg
     6541 catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat
     6601 ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg
     6661 ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc
     6721 tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta
     6781 tcgccttctt gacgagttct tctgagcggg actctggggt tcgaaatgac cgaccaagcg
     6841 acgcccaacc tgccatcacg agatttcgat tccaccgccg ccttctatga aaggttgggc
     6901 ttcggaatcg ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg
     6961 gagttcttcg cccaccccaa cttgtttatt gcagcttata atggttacaa ataaagcaat
     7021 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
     7081 aaactcatca atgtatctta tcatgtctgt ataccgtcga cctctagcta gagcttggcg
     7141 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
     7201 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
     7261 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat
     7321 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
     7381 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     7441 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     7501 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     7561 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     7621 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     7681 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     7741 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     7801 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     7861 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     7921 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     7981 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     8041 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     8101 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     8161 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     8221 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     8281 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     8341 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     8401 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
     8461 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     8521 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     8581 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
     8641 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     8701 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     8761 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     8821 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     8881 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
     8941 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     9001 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     9061 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     9121 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     9181 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     9241 tgtatttaga aaaataaaca aataggggtt cc
//
