LOCUS       sequence-370122-        9405 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses human beta 2 integrin tension sensor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9405)
  AUTHORS   Nordenfelt P, Elliott HL, Springer TA
  TITLE     Coordinated integrin activation by actin-dependent force during 
            T-cell migration.
  JOURNAL   Nat Commun. 2016 Oct 10;7:13119. doi: 10.1038/ncomms13119.
  PUBMED    27721490
REFERENCE   2  (bases 1 to 9405)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-10-10"; volume: "7:13119. doi"; pages: " 
            10.1038/ncomms13119"
COMMENT     Sequence Label: pcDNA3.1/Hygro(-)-B2-TS5
FEATURES             Location/Qualifiers
     source          1..9405
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     629..648
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        629..647
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2914..3621
                     /codon_start=1
                     /product="monomeric teal (cyan) fluorescent protein (Ai et 
                     al., 2006)"
                     /label=mTFP1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEETTMGVIKPDMKIKLKMEGNVNGHAFVIEGEGEGKPYDG
                     TNTINLEVKEGAPLPFSYDILTTAFAYGNRAFTKYPDDIPNYFKQSFPEGYSWERTMTF
                     EDKGIVKVKSDISMEEDSFIYEIHLKGENFPPNGPVMQKKTTGWDASTERMYVRDGVLK
                     GDVKHKLLLEGGGHHRVDFKTIYRAKKAVKLPDYHFVDHRIEILNHDKDYNKVTVYESA
                     VARNSTDGMDELYK"
     CDS             join(3742..3744,3745..3747,3748..4458)
                     /codon_start=1
                     /product="Venus YFP with monomerizing A206K mutation (Nagai
                     et al., 2002; Kremers et al., 2006)"
                     /label=mVenus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3787..3808)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4048..4067)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4395..4416
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(4593..4610)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4599..4823
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4869..5297
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4956..4975)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5166..5187
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5306..5326)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5311..5640
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5491..5626
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5553..5572
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5689..6714
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             5689..6714
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     polyA_signal    6844..6965
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6881..6900)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6935..6954
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7014..7030)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7014..7030)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7027..7049)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7038..7054
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7062..7068,7069..7086,7087..7092))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7107..7128
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7245..7262)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7416..8004)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7496..7515)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8175..8966,8967..9035))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8798..8817
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9036..9140)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9215..9234)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctctgg ctaactagag
      601 aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag acccaagctg
      661 gctagcgcca ccatgctggg cctgcgcccc ccactgctcg ccctggtggg gctgctctcc
      721 ctcgggtgcg tcctctctca ggagtgcacg aagttcaagg tcagcagctg ccgggaatgc
      781 atcgagtcgg ggcccggctg cacctggtgc cagaagctga acttcacagg gccgggggat
      841 cctgactcca ttcgctgcga cacccggcca cagctgctca tgaggggctg tgcggctgac
      901 gacatcatgg accccacaag cctcgctgaa acccaggaag accacaatgg gggccagaag
      961 cagctgtccc cacaaaaagt gacgctttac ctgcgaccag gccaggcagc agcgttcaac
     1021 gtgaccttcc ggcgggccaa gggctacccc atcgacctgt actatctgat ggacctctcc
     1081 tactccatgc ttgatgacct caggaatgtc aagaagctag gtggcgacct gctccgggcc
     1141 ctcaacgaga tcaccgagtc cggccgcatt ggcttcgggt ccttcgtgga caagaccgtg
     1201 ctgccgttcg tgaacacgca ccctgataag ctgcgaaacc catgccccaa caaggagaaa
     1261 gagtgccagc ccccgtttgc cttcaggcac gtgctgaagc tgaccaacaa ctccaaccag
     1321 tttcagaccg aggtcgggaa gcagctgatt tccggaaacc tggatgcacc cgagggtggg
     1381 ctggacgcca tgatgcaggt cgccgcctgc ccggaggaaa tcggctggcg caacgtcacg
     1441 cggctgctgg tgtttgccac tgatgacggc ttccatttcg cgggcgacgg aaagctgggc
     1501 gccatcctga cccccaacga cggccgctgt cacctggagg acaacttgta caagaggagc
     1561 aacgaattcg actacccatc ggtgggccag ctggcgcaca agctggctga aaacaacatc
     1621 cagcccatct tcgcggtgac cagtaggatg gtgaagacct acgagaaact caccgagatc
     1681 atccccaagt cagccgtggg ggagctgtct gaggactcca gcaatgtggt ccatctcatt
     1741 aagaatgctt acaataaact ctcctccagg gtcttcctgg atcacaacgc cctccccgac
     1801 accctgaaag tcacctacga ctccttctgc agcaatggag tgacgcacag gaaccagccc
     1861 agaggtgact gtgatggcgt gcagatcaat gtcccgatca ccttccaggt gaaggtcacg
     1921 gccacagagt gcatccagga gcagtcgttt gtcatccggg cgctgggctt cacggacata
     1981 gtgaccgtgc aggttcttcc ccagtgtgag tgccggtgcc gggaccagag cagagaccgc
     2041 agcctctgcc atggcaaggg cttcttggag tgcggcatct gcaggtgtga cactggctac
     2101 attgggaaaa actgtgagtg ccagacacag ggccggagca gccaggagct ggaaggaagc
     2161 tgccggaagg acaacaactc catcatctgc tcagggctgg gggactgtgt ctgcgggcag
     2221 tgcctgtgcc acaccagcga cgtccccggc aagctgatat acgggcagta ctgcgagtgt
     2281 gacaccatca actgtgagcg ctacaacggc caggtctgcg gcggcccggg gagggggctc
     2341 tgcttctgcg ggaagtgccg ctgccacccg ggctttgagg gctcagcgtg ccagtgcgag
     2401 aggaccactg agggctgcct gaacccgcgg cgtgttgagt gtagtggtcg tggccggtgc
     2461 cgctgcaacg tatgcgagtg ccattcaggc taccagctgc ctctgtgcca ggagtgcccc
     2521 ggctgcccct caccctgtgg caagtacatc tcctgcgccg agtgcctgaa gttcgaaaag
     2581 ggcccctttg ggaagaactg cagcgcggcg tgtccgggcc tgcagctgtc gaacaacccc
     2641 gtgaagggca ggacctgcaa ggagagggac tcagagggct gctgggtggc ctacacgctg
     2701 gagcagcagg acgggatgga ccgctacctc atctatgtgg atgagagccg agagtgtgtg
     2761 gcaggcccca acatcgccgc catcgtcggg ggcaccgtgg caggcatcgt gctgatcggc
     2821 attctcctgc tggtcatctg gaaggctctg atccacctga gcgacctccg ggagtacagg
     2881 cgctttgaga aggagaagct ccaggctcag gcgatggtga gcaagggcga ggagaccaca
     2941 atgggcgtaa tcaagcccga catgaagatc aagctgaaga tggagggcaa cgtgaatggc
     3001 cacgccttcg tgatcgaggg cgagggcgag ggcaagccct acgacggcac caacaccatc
     3061 aacctggagg tgaaggaggg agcccccctg cccttctcct acgacattct gaccaccgcg
     3121 ttcgcctacg gcaacagggc cttcaccaag taccccgacg acatccccaa ctacttcaag
     3181 cagtccttcc ccgagggcta ctcttgggag cgcaccatga ccttcgagga caagggcatc
     3241 gtgaaggtga agtccgacat ctccatggag gaggactcct tcatctacga gatacacctc
     3301 aagggcgaga acttcccccc caacggcccc gtgatgcaga agaagaccac cggctgggac
     3361 gcctccaccg agaggatgta cgtgcgcgac ggcgtgctga agggcgacgt caagcacaag
     3421 ctgctgctgg agggcggcgg ccaccaccgc gttgacttca agaccatcta cagggccaag
     3481 aaggcggtga agctgcccga ctatcacttt gtggaccacc gcatcgagat cctgaaccac
     3541 gacaaggact acaacaaggt gaccgtttac gagagcgccg tggcccgcaa ctccaccgac
     3601 ggcatggacg agctgtacaa ggggccaggt ggtgcagggc caggtggtgc agggccaggt
     3661 ggtgcagggc caggtggtgc agggcccggt ggtgcaggtc caggtggtgc aggtccaggt
     3721 ggtgcaggtc caggtggtgc tatggtgagc aagggcgagg agctgttcac cggggtggtg
     3781 cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag
     3841 ggcgagggcg atgccaccta cggcaagctg accctgaagc tgatctgcac caccggcaag
     3901 ctgcccgtgc cctggcccac cctcgtgacc accctgggct acggcctgca gtgcttcgcc
     3961 cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac
     4021 gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg
     4081 aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag
     4141 gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc
     4201 accgccgaca agcagaagaa cggcatcaag gccaacttca agatccgcca caacatcgag
     4261 gacggcggcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc
     4321 gtgctgctgc ccgacaacca ctacctgagc taccagtcca aactgagcaa agaccccaac
     4381 gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc
     4441 atggacgagc tgtacaaggc tcaagcacaa aaggagaagc tcaagtccca gtggaacaat
     4501 gataatcccc ttttcaagag cgccaccacg acggtcatga accccaagtt tgctgagagt
     4561 tagtaaaagc ttaagtttaa accgctgatc agcctcgact gtgccttcta gttgccagcc
     4621 atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt
     4681 cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct
     4741 ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc
     4801 tggggatgcg gtgggctcta tggcttctga ggcggaaaga accagctggg gctctagggg
     4861 gtatccccac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag
     4921 cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt
     4981 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt
     5041 ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg
     5101 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt
     5161 taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt
     5221 tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca
     5281 aaaatttaac gcgaattaat tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca
     5341 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt
     5401 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca
     5461 gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc
     5521 cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctct
     5581 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa
     5641 aagctcccgg gagcttgtat atccattttc ggatctgatc agcacgtgat gaaaaagcct
     5701 gaactcaccg cgacgtctgt cgagaagttt ctgatcgaaa agttcgacag cgtctccgac
     5761 ctgatgcagc tctcggaggg cgaagaatct cgtgctttca gcttcgatgt aggagggcgt
     5821 ggatatgtcc tgcgggtaaa tagctgcgcc gatggtttct acaaagatcg ttatgtttat
     5881 cggcactttg catcggccgc gctcccgatt ccggaagtgc ttgacattgg ggaattcagc
     5941 gagagcctga cctattgcat ctcccgccgt gcacagggtg tcacgttgca agacctgcct
     6001 gaaaccgaac tgcccgctgt tctgcagccg gtcgcggagg ccatggatgc gatcgctgcg
     6061 gccgatctta gccagacgag cgggttcggc ccattcggac cgcaaggaat cggtcaatac
     6121 actacatggc gtgatttcat atgcgcgatt gctgatcccc atgtgtatca ctggcaaact
     6181 gtgatggacg acaccgtcag tgcgtccgtc gcgcaggctc tcgatgagct gatgctttgg
     6241 gccgaggact gccccgaagt ccggcacctc gtgcacgcgg atttcggctc caacaatgtc
     6301 ctgacggaca atggccgcat aacagcggtc attgactgga gcgaggcgat gttcggggat
     6361 tcccaatacg aggtcgccaa catcttcttc tggaggccgt ggttggcttg tatggagcag
     6421 cagacgcgct acttcgagcg gaggcatccg gagcttgcag gatcgccgcg gctccgggcg
     6481 tatatgctcc gcattggtct tgaccaactc tatcagagct tggttgacgg caatttcgat
     6541 gatgcagctt gggcgcaggg tcgatgcgac gcaatcgtcc gatccggagc cgggactgtc
     6601 gggcgtacac aaatcgcccg cagaagcgcg gccgtctgga ccgatggctg tgtagaagta
     6661 ctcgccgata gtggaaaccg acgccccagc actcgtccga gggcaaagga atagcacgtg
     6721 ctacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc
     6781 cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac
     6841 cccaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
     6901 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
     6961 tcttatcatg tctgtatacc gtcgacctct agctagagct tggcgtaatc atggtcatag
     7021 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc
     7081 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
     7141 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
     7201 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
     7261 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     7321 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     7381 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     7441 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     7501 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     7561 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     7621 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     7681 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     7741 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     7801 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
     7861 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     7921 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     7981 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     8041 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     8101 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     8161 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     8221 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     8281 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
     8341 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     8401 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     8461 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
     8521 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
     8581 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
     8641 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
     8701 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
     8761 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
     8821 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
     8881 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
     8941 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
     9001 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
     9061 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
     9121 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt cgacggatcg
     9181 ggagatctcc cgatccccta tggtgcactc tcagtacaat ctgctctgat gccgcatagt
     9241 taagccagta tctgctccct gcttgtgtgt tggaggtcgc tgagtagtgc gcgagcaaaa
     9301 tttaagctac aacaaggcaa ggcttgaccg acaattgcat gaagaatctg cttagggtta
     9361 ggcgttttgc gctgcttcgc gatgtacggg ccagatatac gcgtt
//
