LOCUS       sequence-370131-        9228 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  TFEB - Lysosomal biogenesis gene reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9228)
  AUTHORS   O'Connell DJ, Kolde R, Sooknah M, Graham DB, Sundberg TB, Latorre 
            IJ, Mikkelsen TS, Xavier RJ
  TITLE     Simultaneous Pathway Activity Inference and Gene Expression Analysis
            Using RNA Sequencing.
  JOURNAL   Cell Syst. 2016 May 25;2(5):323-34. doi: 10.1016/j.cels.2016.04.011.
            Epub 2016 May 19.
  PUBMED    27211859
REFERENCE   2  (bases 1 to 9228)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cels.2016.04.011"; journalName: "Cell Syst"; date: 
            "2016-05-25- 25"; volume: "2"; issue: "5"; pages: "323-34"
COMMENT     Sequence Label: pLminP_Luc2P_RE28
FEATURES             Location/Qualifiers
     source          1..9228
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        35..351
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      202..337
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             join(402..404,405..407,408..1121)
                     /codon_start=1
                     /product="Emerald GFP"
                     /label=EmGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(447..468)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(708..727)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1055..1076
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     LTR             1193..1426
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    1498..1619
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1535..1554)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1589..1608
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      1659..1794
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(1814..1833)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1815..1833)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1843..1860)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1843..1859)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1852..1874)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      2001..2456
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2088..2107)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2298..2319
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        2482..2586
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2587..2655,2656..3447)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2805..2824)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3618..4206
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4107..4126
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4360..4377
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4494..4515
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4530..4535,4536..4553,4554..4560)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4568..4584
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4573..4595
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4592..4608
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4592..4608
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     4627..4647
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        4629..4647
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        4675..4901
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             4902..5082
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    5129..5254
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    5747..5980
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             6165..6209
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    6476..6593
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        6732..6763
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     regulatory      6790..6799
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6796..8445
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     CDS             8449..8568
                     /codon_start=1
                     /product="PEST degradation sequence from mouse ornithine 
                     decarboxylase"
                     /label=hPEST
                     /note="human codon-optimized"
                     /translation="SHGFPPEVEEQAAGTLPMSCAQESGMDRHPAACASARINV"
     misc_feature    join(8660..9228,1..20)
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8713..8733)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9131..9142)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 cctttgggcc gcctccccgc ctggcgatgg taccggtgtg gaaagtcccc aggctcccca
       61 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc
      121 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata
      181 gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
      241 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc tgcctctgag
      301 ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctcccg
      361 ggagcttgta tatccatttt cggatctgat cagcacctag catggtgagc aagggcgagg
      421 agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca
      481 agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt
      541 tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accttcacct
      601 acggcgtgca gtgcttcgcc cgctaccccg accacatgaa gcagcacgac ttcttcaagt
      661 ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact
      721 acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga
      781 agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca
      841 acagccacaa ggtctatatc accgccgaca agcagaagaa cggcatcaag gtgaacttca
      901 agacccgcca caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca
      961 cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc acccagtccg
     1021 ccctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg
     1081 ccgccgggat cactctcggc atggacgagc tgtacaagta aggtaccttt aagaccaatg
     1141 acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg
     1201 ctaattcact cccaacgaag acaagatctg ctttttgctt gtactgggtc tctctggtta
     1261 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
     1321 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
     1381 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtag tagttcatgt
     1441 catcttatta ttcagtattt ataacttgca aagaaatgaa tatcagagag tgagaggaac
     1501 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
     1561 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
     1621 catgtctggc tctagctatc ccgcccctaa ctccgcccat cccgccccta actccgccca
     1681 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     1741 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggga
     1801 cgtacccaat tcgccctata gtgagtcgta ttacgcgcgc tcactggccg tcgttttaca
     1861 acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc
     1921 tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg
     1981 cagcctgaat ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt
     2041 ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt
     2101 cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct
     2161 ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg
     2221 tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga
     2281 gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc
     2341 ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga
     2401 gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgctta caatttaggt
     2461 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
     2521 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
     2581 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc
     2641 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg
     2701 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt
     2761 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta
     2821 ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat
     2881 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga
     2941 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca
     3001 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact
     3061 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc
     3121 acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact
     3181 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt
     3241 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt
     3301 gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt
     3361 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
     3421 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag
     3481 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
     3541 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     3601 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     3661 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     3721 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
     3781 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     3841 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     3901 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     3961 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     4021 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     4081 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     4141 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     4201 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     4261 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
     4321 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
     4381 gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc
     4441 agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg
     4501 agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg
     4561 tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgcc
     4621 aagcgcgcaa ttaaccctca ctaaagggaa caaaagctgg agctgcaagc ttaatgtagt
     4681 cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa catgccttac
     4741 aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac gatcgtgcct
     4801 tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa ttgccgcatt
     4861 gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc tggttagacc
     4921 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa
     4981 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga
     5041 gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc cgaacaggga
     5101 cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc ttgctgaagc
     5161 gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg
     5221 aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga gaattagatc
     5281 gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat taaaacatat
     5341 agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt tagaaacatc
     5401 agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag gatcagaaga
     5461 acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa ggatagagat
     5521 aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa gtaagaccac
     5581 cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg gacaattgga
     5641 gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta gcacccacca
     5701 aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga gctttgttcc
     5761 ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac
     5821 aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg agggctattg
     5881 aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc caggcaagaa
     5941 tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg ggttgctctg
     6001 gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat aaatctctgg
     6061 aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac aattacacaa
     6121 gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat gaacaagaat
     6181 tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca aattggctgt
     6241 ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga atagtttttg
     6301 ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg tttcagaccc
     6361 acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa ggtggagaga
     6421 gagacagaga cagatccatt cgattagtga acggatctcg acggtatcgg ttaactttta
     6481 aaagaaaagg ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa
     6541 cagacataca aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttatcgatc
     6601 gcaccagcgt gtggatcgta ggccacgtga ccggggtagg ccacgtgacc ggggtaggcc
     6661 acgtgaccgg ggtaggccac gtgaccgggg atccgagaac agatctggcc tcggcggcca
     6721 agcttagaca ctagagggta tataatggaa gctcgacttc cagcttggca atccggtact
     6781 gttggtaaag ccaccatgga agatgccaaa aacattaaga agggcccagc gccattctac
     6841 ccactcgaag acgggaccgc cggcgagcag ctgcacaaag ccatgaagcg ctacgccctg
     6901 gtgcccggca ccatcgcctt taccgacgca catatcgagg tggacattac ctacgccgag
     6961 tacttcgaga tgagcgttcg gctggcagaa gctatgaagc gctatgggct gaatacaaac
     7021 catcggatcg tggtgtgcag cgagaatagc ttgcagttct tcatgcccgt gttgggtgcc
     7081 ctgttcatcg gtgtggctgt ggccccagct aacgacatct acaacgagcg cgagctgctg
     7141 aacagcatgg gcatcagcca gcccaccgtc gtattcgtga gcaagaaagg gctgcaaaag
     7201 atcctcaacg tgcaaaagaa gctaccgatc atacaaaaga tcatcatcat ggatagcaag
     7261 accgactacc agggcttcca aagcatgtac accttcgtga cttcccattt gccacccggc
     7321 ttcaacgagt acgacttcgt gcccgagagc ttcgaccggg acaaaaccat cgccctgatc
     7381 atgaacagta gtggcagtac cggattgccc aagggcgtag ccctaccgca ccgcaccgct
     7441 tgtgtccgat tcagtcatgc ccgcgacccc atcttcggca accagatcat ccccgacacc
     7501 gctatcctca gcgtggtgcc atttcaccac ggcttcggca tgttcaccac gctgggctac
     7561 ttgatctgcg gctttcgggt cgtgctcatg taccgcttcg aggaggagct attcttgcgc
     7621 agcttgcaag actataagat tcaatctgcc ctgctggtgc ccacactatt tagcttcttc
     7681 gctaagagca ctctcatcga caagtacgac ctaagcaact tgcacgagat cgccagcggc
     7741 ggggcgccgc tcagcaagga ggtaggtgag gccgtggcca aacgcttcca cctaccaggc
     7801 atccgccagg gctacggcct gacagaaaca accagcgcca ttctgatcac ccccgaaggg
     7861 gacgacaagc ctggcgcagt aggcaaggtg gtgcccttct tcgaggctaa ggtggtggac
     7921 ttggacaccg gtaagacact gggtgtgaac cagcgcggcg agctgtgcgt ccgtggcccc
     7981 atgatcatga gcggctacgt taacaacccc gaggctacaa acgctctcat cgacaaggac
     8041 ggctggctgc acagcggcga catcgcctac tgggacgagg acgagcactt cttcatcgtg
     8101 gaccggctga agagcctgat caaatacaag ggctaccagg tagccccagc cgaactggag
     8161 agcatcctgc tgcaacaccc caacatcttc gacgccgggg tcgccggcct gcccgacgac
     8221 gatgccggcg agctgcccgc cgcagtcgtc gtgctggaac acggtaaaac catgaccgag
     8281 aaggagatcg tggactatgt ggccagccag gttacaaccg ccaagaagct gcgcggtggt
     8341 gttgtgttcg tggacgaggt gcctaaagga ctgaccggca agttggacgc ccgcaagatc
     8401 cgcgagattc tcattaaggc caagaagggc ggcaagatcg ccgtgaattc tcacggcttc
     8461 cctcccgagg tggaggagca ggccgccggc accctgccca tgagctgcgc ccaggagagc
     8521 ggcatggata gacaccctgc tgcttgcgcc agcgccagga tcaacgtcta aggccgcgac
     8581 tctagaacgc gtcactgcgg ctagtataga tgggtgcatg actacagaca tcagcgcgta
     8641 ccggttagta atgatcgaca atcaacctct ggattacaaa atttgtgaaa gattgactgg
     8701 tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta
     8761 tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct
     8821 gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt
     8881 tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac
     8941 tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg
     9001 ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaagctgac
     9061 gtcctttcca tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg
     9121 ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct
     9181 gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctc
//
