LOCUS       sequence-370132-       12370 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  PROSS-designed high-redox potential laccase from Trametes hirsuta.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12370)
  AUTHORS   Barber-Zucker S, Mateljak I, Goldsmith M, Kupervaser M, Alcalde M, 
            Fleishman SJ
  TITLE     Designed High-Redox Potential Laccases Exhibit High Functional 
            Diversity.
  JOURNAL   ACS Catal. 2022 Nov 4;12(21):13164-13173. doi: 
            10.1021/acscatal.2c03006. Epub 2022 Oct 13.
  PUBMED    36366766
REFERENCE   2  (bases 1 to 12370)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acscatal.2c03006"; journalName: "ACS Catal"; date: 
            "2022-11-4- 4"; volume: "12"; issue: "21"; pages: "13164-13173"
COMMENT     Sequence Label: pJRoC30_Th3
FEATURES             Location/Qualifiers
     source          1..12370
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             783..2054
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=FLP
                     /note="FLP is a site-specific recombinase from 
                     Saccharomyces cerevisiae. Recombination occurs at FRT 
                     sequences."
                     /translation="MPQFDILCKTPPKVLVRQFVERFERPSGEKIALCAAELTYLCWMI
                     THNGTAIKRATFMSYNTIISNSLSFDIVNKSLQFKYKTQKATILEASLKKLIPAWEFTI
                     IPYYGQKHQSDITDIVSSLQLQFESSEEADKGNSHSKKMLKALLSEGESIWEITEKILN
                     SFEYTSRFTKTKTLYQFLFLATFINCGRFSDIKNVDPKSFKLVQNKYLGVIIQCLVTET
                     KTSVSRHIYFFSARGRIDPLVYLDEFLRNSEPVLKRVNRTGNSSSNKQEYQLLKDNLVR
                     SYNKALKKNAPYSIFAIKNGPKSHIGRHLMTSFLSMKGLTELTNVVGNWSDKRASAVAR
                     TTYTHQITAIPDHYFALVSRYYAYDPISKEMIALKDETNPIEEWQHIEQLKGSAEGSIR
                     YPAWNGIISQEVLDYLSSYINRRI"
     protein_bind    2207..2254
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     promoter        5154..5369
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     CDS             5370..6173
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     CDS             join(6269..6337,6338..7129)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6487..6506)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7300..7888
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7789..7808
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8042..8059
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(8136..8383)
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     8349..8367
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     CDS             complement(8420..8437)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(8444..8461)
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             complement(join(9974..9985,9986..10183,10184..10240))
                     /codon_start=1
                     /gene="MF-alpha-1"
                     /product="N-terminal secretion signal from S. cerevisiae 
                     alpha-factor"
                     /label=alpha-factor secretion signal
                     /note="Cleavage by the Kex2 protease occurs after the 
                     dibasic KR sequence. The EA dipeptides are then removed by 
                     dipeptidyl aminopeptidase A."
                     /translation="MRFPSIFTADLFAASSALAAPVKTTTEDETAQIPAEAVIGYSDLE
                     GDFDVAVLPFSNSTNNGLLFINTTIASIAAKEEGVSLEKRETEA"
     promoter        complement(10266..10707)
                     /gene="S. cerevisiae GAL1"
                     /label=GAL1 promoter
                     /note="inducible promoter, regulated by Gal4"
     primer_bind     complement(10272..10295)
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     protein_bind    10590..10707
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     rep_origin      10729..11185
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10817..10836)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     11027..11048
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     protein_bind    complement(11513..11560)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
ORIGIN
        1 agcaatagat ttgtatagtt gctttctatt gtctaacagc ttgttattct gtagcatcaa
       61 atctatggca gcctgacttg cttcttgtga agagagcata ccatttccaa tcgaatcaaa
      121 cctttcctta accatcttcg cagcaggcaa aattacctca gcactggagt cagaagatac
      181 gctggaatct tctgcgctag aatcaagacc atacggccta ccggttgtga gagattccat
      241 gggccttatg acatatcctg gaaagagtag ctcatcagac ttacgtttac tctctatatc
      301 aatatctaca tcaggagcaa tcatttcaat aaacagccga catacatccc agacgctata
      361 agctgtacgt gcttttaccg tcagattctt ggctgtttca atgtcgtcca ttttggtttt
      421 cttttaccag tattgttcgt ttgataatgt attcttgctt attacattat aaaatctgtg
      481 cagatcacat gtcaaaacaa ctttttatca caagatagta ccgcaaaacg aacctgcggg
      541 ccgtctaaaa attaaggaaa agcagcaaag gtgcattttt aaaatatgaa atgaagatac
      601 cgcagtacca attattttcg cagtacaaat aatgcgcggc cggtgcattt ttcgaaagaa
      661 cgcgagacaa acaggacaat taaagttagt ttttcgagtt agcgtgtttg aatactgcaa
      721 gatacaagat aaatagagta gttgaaacta gatatcaatt gcacacaaga tcggcgctaa
      781 gcatgccaca atttgatata ttatgtaaaa caccacctaa ggtgcttgtt cgtcagtttg
      841 tggaaaggtt tgaaagacct tcaggtgaga aaatagcatt atgtgctgct gaactaacct
      901 atttatgttg gatgattaca cataacggaa cagcaatcaa gagagccaca ttcatgagct
      961 ataatactat cataagcaat tcgctgagtt tcgatattgt caataaatca ctccagttta
     1021 aatacaagac gcaaaaagca acaattctgg aagcctcatt aaagaaattg attcctgctt
     1081 gggaatttac aattattcct tactatggac aaaaacatca atctgatatc actgatattg
     1141 taagtagttt gcaattacag ttcgaatcat cggaagaagc agataaggga aatagccaca
     1201 gtaaaaaaat gcttaaagca cttctaagtg agggtgaaag catctgggag atcactgaga
     1261 aaatactaaa ttcgtttgag tatacttcga gatttacaaa aacaaaaact ttataccaat
     1321 tcctcttcct agctactttc atcaattgtg gaagattcag cgatattaag aacgttgatc
     1381 cgaaatcatt taaattagtc caaaataagt atctgggagt aataatccag tgtttagtga
     1441 cagagacaaa gacaagcgtt agtaggcaca tatacttctt tagcgcaagg ggtaggatcg
     1501 atccacttgt atatttggat gaatttttga ggaattctga accagtccta aaacgagtaa
     1561 ataggaccgg caattcttca agcaataaac aggaatacca attattaaaa gataacttag
     1621 tcagatcgta caataaagct ttgaagaaaa atgcgcctta ttcaatcttt gctataaaaa
     1681 atggcccaaa atctcacatt ggaagacatt tgatgacctc atttctttca atgaagggcc
     1741 taacggagtt gactaatgtt gtgggaaatt ggagcgataa gcgtgcttct gccgtggcca
     1801 ggacaacgta tactcatcag ataacagcaa tacctgatca ctacttcgca ctagtttctc
     1861 ggtactatgc atatgatcca atatcaaagg aaatgatagc attgaaggat gagactaatc
     1921 caattgagga gtggcagcat atagaacagc taaagggtag tgctgaagga agcatacgat
     1981 accccgcatg gaatgggata atatcacagg aggtactaga ctacctttca tcctacataa
     2041 atagacgcat ataagtacgc atttaagcat aaacacgcac tatgccgttc ttctcatgta
     2101 tatatatata caggcaacac gcagatatag gtgcgacgtg aacagtgagc tgtatgtgcg
     2161 cagctcgcgt tgcattttcg gaagcgctcg ttttcggaaa cgctttgaag ttcctattcc
     2221 gaagttccta ttctctagaa agtataggaa cttcagagcg cttttgaaaa ccaaaagcgc
     2281 tctgaagacg cactttcaaa aaaccaaaaa cgcaccggac tgtaacgagc tactaaaata
     2341 ttgcgaatac cgcttccaca aacattgctc aaaagtatct ctttgctata tatctctgtg
     2401 ctatatccct atataaccta cccatccacc tttcgctcct tgaacttgca tctaaactcg
     2461 acctctacat caacaggctt ccaatgctct tcaaatttta ctgtcaagta gacccatacg
     2521 gctgtaatat gctgctcttc ataatgtaag cttatcttta tcgaatcgtg tgaaaaacta
     2581 ctaccgcgat aaacctttac ggttccctga gattgaatta gttcctttag tatatgatac
     2641 aagacacttt tgaactttgt acgacgaatt ttgaggttcg ccatcctctg gctatttcca
     2701 attatcctgt cggctattat ctccgcctca gtttgatctt ccgcttcaga ctgccatttt
     2761 tcacataatg aatctatttc accccacaat ccttcatccg cctccgcatc ttgttccgtt
     2821 aaactattga cttcatgttg tacattgttt agttcacgag aagggtcctc ttcaggcggt
     2881 agctcctgat ctcctatatg acctttatcc tgttctcttt ccacaaactt agaaatgtat
     2941 tcatgaatta tggagcacct aataacattc ttcaaggcgg agaagtttgg gccagatgcc
     3001 caatatgctt gacatgaaaa cgtgagaatg aatttagtat tattgtgata ttctgaggca
     3061 attttattat aatctcgaag ataagagaag aatgcagtga cctttgtatt gacaaatgga
     3121 gattccatgt atctaaaaaa tacgccttta ggccttctga taccctttcc cctgcggttt
     3181 agcgtgcctt ttacattaat atctaaaccc tctccgatgg tggcctttaa ctgactaata
     3241 aatgcaaccg atataaactg tgataattct gggtgattta tgattcgatc gacaattgta
     3301 ttgtacacta gtgcaggatc aggccaatcc agttcttttt caattaccgg tgtgtcgtct
     3361 gtattcagta catgtccaac aaatgcaaat gctaacgttt tgtatttctt ataattgtca
     3421 ggaactggaa aagtccccct tgtcgtctcg attacacacc tactttcatc gtacaccata
     3481 ggttggaagt gctgcataat acattgctta atacaagcaa gcagtctctc gccattcata
     3541 tttcagttat tttccattac agctgatgtc attgtatatc agcgctgtaa aaatctatct
     3601 gttacagaag gttttcgcgg tttttataaa caaaactttc gttacgaaat cgagcaatca
     3661 ccccagctgc gtatttggaa attcgggaaa aagtagagca acgcgagttg cattttttac
     3721 accataatgc atgattaact tcgagaaggg attaaggcta atttcactag tatgtttcaa
     3781 aaacctcaat ctgtccattg aatgccttat aaaacagcta tagattgcat agaagagtta
     3841 gctactcaat gctttttgtc aaagcttact gatgatgatg tgtctacttt caggcgggtc
     3901 tgtagtaagg agaatgacat tataaagctg gcacttagaa ttccacggac tatagactat
     3961 actagtatac tccgtctact gtacgataca cttccgctca ggtccttgtc ctttaacgag
     4021 gccttaccac tcttttgtta ctctattgat ccagctcagc aaaggcagtg tgatctaaga
     4081 ttctatcttc gcgatgtagt aaaactagct agaccgagaa agagactaga aatgcaaaag
     4141 gcacttctac aatggctgcc atcattatta tccgatgtga cgctgcagct tctcaatgat
     4201 attcgaatac gctttgagga gatacagcct aatatccgac aaactgtttt acagatttac
     4261 gatcgtactt gttacccatc attgaatttt gaacatccga acctgggagt tttccctgaa
     4321 acagatagta tatttgaacc tgtataataa tatatagtct agcgctttac ggaagacaat
     4381 gtatgtattt cggttcctgg agaaactatt gcatctattg cataggtaat cttgcacgtc
     4441 gcatccccgg ttcattttct gcgtttccat cttgcacttc aatagcatat ctttgttaac
     4501 gaagcatctg tgcttcattt tgtagaacaa aaatgcaacg cgagagcgct aatttttcaa
     4561 acaaagaatc tgagctgcat ttttacagaa cagaaatgca acgcgaaagc gctattttac
     4621 caacgaagaa tctgtgcttc atttttgtaa aacaaaaatg caacgcgaga gcgctaattt
     4681 ttcaaacaaa gaatctgagc tgcattttta cagaacagaa atgcaacgcg agagcgctat
     4741 tttaccaaca aagaatctat acttcttttt tgttctacaa aaatgcatcc cgagagcgct
     4801 atttttctaa caaagcatct tagattactt tttttctcct ttgtgcgctc tataatgcag
     4861 tctcttgata actttttgca ctgtaggtcc gttaaggtta gaagaaggct actttggtgt
     4921 ctattttctc ttccataaaa aaagcctgac tccacttccc gcgtttactg attactagcg
     4981 aagctgcggg tgcatttttt caagataaag gcatccccga ttatattcta taccgatgtg
     5041 gattgcgcat actttgtgaa cagaaagtga tagcgttgat gattcttcat tggtcagaaa
     5101 attatgaacg gtttcttcta ttttgtctct atatactacg tacctgctag cttttcaatt
     5161 catcattttt tttttattct tttttttgat ttcggtttcc ttgaaatttt tttgattcgg
     5221 taatctccga acagaaggaa gaacgaagga aggagcacag acttagattg gtatatatac
     5281 gcatatgtag tgttgaagaa acatgaaatt gcccagtatt cttaacccaa ctgcacagaa
     5341 caaaaacctg caggaaacga agataaatca tgtcgaaagc tacatataag gaacgtgctg
     5401 ctactcatcc tagtcctgtt gctgccaagc tatttaatat catgcacgaa aagcaaacaa
     5461 acttgtgtgc ttcattggat gttcgtacca ccaaggaatt actggagtta gttgaagcat
     5521 taggtcccaa aatttgttta ctaaaaacac atgtggatat cttgactgat ttttccatgg
     5581 agggcacagt taagccgcta aaggcattat ccgccaagta caatttttta ctcttcgaag
     5641 acagaaaatt tgctgacatt ggtaatacag tcaaattgca gtactctgcg ggtgtataca
     5701 gaatagcaga atgggcagac attacgaatg cacacggtgt ggtgggccca ggtattgtta
     5761 gcggtttgaa gcaggcggca gaagaagtaa caaaggaacc tagaggcctt ttgatgttag
     5821 cagaattgtc atgcaagggc tccctatcta ctggagaata tactaagggt actgttgaca
     5881 ttgcgaagag cgacaaagat tttgttatcg gctttattgc tcaaagagac atgggtggaa
     5941 gagatgaagg ttacgattgg ttgattatga cacccggtgt gggtttagat gacaagggag
     6001 acgcattggg tcaacagtat agaaccgtgg atgatgtggt ctctacagga tctgacatta
     6061 ttattgttgg aagaggacta tttgcaaagg gaagggatgc taaggtagag ggtgaacgtt
     6121 acagaaaagc aggctgggaa gcatatttga gaagatgcgg ccagcaaaac taaaaaactg
     6181 tattataagt aaatgcatgt atactaaact cacaaattag agcttcaatt taattatatc
     6241 agttattacc cattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
     6301 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
     6361 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
     6421 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
     6481 agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg
     6541 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
     6601 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
     6661 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
     6721 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
     6781 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact
     6841 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
     6901 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
     6961 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
     7021 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
     7081 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
     7141 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     7201 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     7261 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     7321 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     7381 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     7441 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     7501 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     7561 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     7621 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     7681 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     7741 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     7801 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     7861 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     7921 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
     7981 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
     8041 cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc
     8101 gcgttggccg attcattaat gcagggccgc agcttgcaaa ttaaagcctt cgagcgtccc
     8161 aaaaccttct caagcaaggt tttcagtata atgttacatg cgtacacgcg tctgtacaga
     8221 aaaaaaagaa aaatttgaaa tataaataac gttctcaata ctaacataac tataaaaaaa
     8281 taaataggga cctagacttc aggttgtcta actccttcct tttcggttag agcggatgtg
     8341 gggggagggc gtgaatgtaa gcgtgacata actaattaca tgatgcggcc ctctagatgc
     8401 atgctcgagc ggccgcttag tggtggtggt ggtggtgcgc gctgctgccg cggggaacta
     8461 aaccgctgct ctgatcgttt gggtccaagg catcgtaagt cgggcataag tcactccagg
     8521 cctgagggac tgggttcaca gcctttacgt cgggcaggtc ctcggccatt actaccgcga
     8581 aaccagcctc taaatggaag tcaatatggc agtgtaggaa ccacggacca ggattattag
     8641 tgtcgaatct aatggtcacg ttgtctccag ccgcaggagt accagtagaa actacgtccc
     8701 tgaaaatagg attgtcgtag ttataaaccg ttgagcccgc tgacctgact accgcgaaag
     8761 tgtgaccgtg caggtggaaa gggtggggtg caccgggggc tgcggcagtt gcaggaaaac
     8821 taatctctat ggtcgcgttt gatggcagta cataaaccga cccactaggc aataaatcct
     8881 gcgcagtttg tgcacctgaa aggatctgta atagaacagg cacggttggt ggtacgaacg
     8941 acacaccatt gatgaagaag ttactaccgt taaagttaaa tgccatgttg atggctttat
     9001 ctacgccacc gggactcgga gacccaggaa caggtgttgg tactagtggg tgtaagtcag
     9061 tttcgtttag gggcttaacc gaggttgttt gattggtggt aggctccact gcgggtgcac
     9121 cgtcgtatct taatattgcg gaatttatgc caccgtcaaa accaacgttg ccgaaattag
     9181 ggttcgccct tatccagtag ttgtcaactg gctggttggc gtctaagacg aaggagtaac
     9241 gttgggcagc aaatatctgt atactatcca cttctagcgg ctgtacatta acgctgtcga
     9301 cctcaataat cgttaaattg tgaccatcaa tactaaacgt gaaatttgga ttgcaagaca
     9361 ggctaaccag cctgaaccta tacctcttac ctttcgttac cttaatgact gataactcgg
     9421 ctgttggatc agacggggct ctccccttcc cgttaatcag agttgcatct gcgccaggtg
     9481 gaaacctggg tcccaacttt gcagccgtgt ggtaccagtc cgccagtgtt attactgtgc
     9541 tgtcattatc tacatcatac atactagcgt gtgggtcgtt tgggtcatac acaacaaaag
     9601 ggccccttaa tccatcgcag tactgcgtgc ttagatgaga atgataccaa aaggtacccg
     9661 cttggtcagg tacttggaaa tcatatagaa atgaatgccc cggtgctatt gggcattggt
     9721 ttatgaaagc tgggccgtcg gcccaattcg ttccgtgctg gaagaatccg tgccagtgaa
     9781 tagaagtaga ttttagcatg gtgtgatttg taaggttgtt tattacattt aactggaatc
     9841 tgtcgccctt gttgccggcg attagtggtc cgggcgtcac gccgttgacc acgactgctt
     9901 gccttgagaa accgtcaggg ctcactacgg catctgtgat ggttagatct gcgacggggc
     9961 ctaccgcgaa ctcagcttca gtctctcttt tctcgagaga taccccttct tctttagcag
    10021 caatgctggc aatagtagta tttataaaca ataatccgtt atttgtgctg ttggaaaatg
    10081 gcaaaacagc aacatcgaaa tccccttcta aatctgagta accgatgaca gcttcagccg
    10141 gaatttgtgc cgtttcatct tctgttgtag ttttgactgg agcagctaat gcggaggatg
    10201 ctgcgaataa atcagcagta aaaattgaag gaaatctcat ggatcctatg atcctatagt
    10261 tttttctcct tgacgttaaa gtatagaggt atattaacaa ttttttgttg atacttttat
    10321 tacatttgaa taagaagtaa tacaaaccga aaatgttgaa agtattagtt aaagtggtta
    10381 tgcagttttt gcatttatat atctgttaat agatcaaaaa tcatcgcttc gctgattaat
    10441 taccccagaa ataaggctaa aaaactaatc gcattatcat cctatggttg ttaatttgat
    10501 tcgttcattt gaaggtttgt ggggccaggt tactgccaat ttttcctctt cataaccata
    10561 aaagctagta ttgtagaatc tttattgttc ggagcagtgc ggcgcgaggc acatctgcgt
    10621 ttcaggaacg cgaccggtga agacgaggac gcacggagga gagtcttcct tcggagggct
    10681 gtcacccgct cggcggcttc taatccgtgg actagtggat cgatccccac gcgccctgta
    10741 gcggcgcatt aagcgcggcg ggtgtggtgg nttacgcgca gcgtgaccgc tacacttgcc
    10801 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
    10861 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg
    10921 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga
    10981 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc
    11041 caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg
    11101 ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt
    11161 aacaaaatat taacgcttac aatttctcta cgtataggaa atgtttacat tttcgtattg
    11221 ttttcgattc actctatgaa tagttcttac tacaattttt ttgtctaaag agtaatacta
    11281 gagataaaca taaaaaatgt agaggtcgag tttagatgca agttcaagga gcgaaaggtg
    11341 gatgggtagg ttatataggg atatagcaca gagatatata gcaaagagat acttttgagc
    11401 aatgtttgtg gaagcggtat tcgcaatatt ttagtagctc gttacagtcc ggtgcgtttt
    11461 tggttttttg aaagtgcgtc ttcagagcgc ttttggtttt caaaagcgct ctgaagttcc
    11521 tatactttct agagaatagg aacttcggaa taggaacttc aaagcgtttc cgaaaacgag
    11581 cgcttccgaa aatgcaacgc gagctgcgca catacagctc actgttcacg tcgcacctat
    11641 atctgcgtgt tgcctgtata tatatataca tgagaagaac ggcatagtgc gtgtttatgc
    11701 ttaaatgcgt acttatatgc gtctatttat gtaggatgaa aggtagtcta gtacctcctg
    11761 tgatattatc ccattccatg cggggtatcg tatgcttcct tcagcactac cctttagctg
    11821 ttctatatgc tgccactcct caattggatt agtctcatcc ttcaatgcta tcatttcctt
    11881 tgatattgga tcatacccta gaagtattac gtgattttct gccccttacc ctcgttgcta
    11941 ctctcctttt tttcgtggga accgctttag ggccctcagt gatggtgttt tgtaatttat
    12001 atgctcctct tgcatttgtg tctctacttc ttgttcgcct ggagggaact tcttcatttg
    12061 tattagcatg gttcacttca gtccttcctt ccaactcact ctttttttgc tgtaaacgat
    12121 tctctgccgc cagttcattg aaactattga atatatcctt tagagattcc gggatgaata
    12181 aatcacctat taaagcagct tgacgatctg gtggaactaa agtaagcaat tgggtaacga
    12241 cgcttacgag cttcataaca tcttcttccg ttggagctgg tgggactaat aactgtgtac
    12301 aatccatttt tctcatgagc atttcggtag ctctcttctt gtctttctcg ggcaatcttc
    12361 ctattattat
//
