LOCUS       sequence-370133-       11838 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  PROSS-designed versatile peroxidase 5H.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11838)
  AUTHORS   Barber-Zucker S, Mindel V, Garcia-Ruiz E, Weinstein JJ, Alcalde M, 
            Fleishman SJ
  TITLE     Stable and Functionally Diverse Versatile Peroxidases Designed 
            Directly from Sequences.
  JOURNAL   J Am Chem Soc. 2022 Mar 2;144(8):3564-3571. doi: 
            10.1021/jacs.1c12433. Epub 2022 Feb 18.
  PUBMED    35179866
REFERENCE   2  (bases 1 to 11838)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/jacs.1c12433"; journalName: "J Am Chem Soc"; date: 
            "2022-03-2- 2"; volume: "144"; issue: "8"; pages: "3564-3571"
COMMENT     Sequence Label: pJRoC30_VP5H
FEATURES             Location/Qualifiers
     source          1..11838
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(62..109)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             1702..2973
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=FLP
                     /note="FLP is a site-specific recombinase from 
                     Saccharomyces cerevisiae. Recombination occurs at FRT 
                     sequences."
                     /translation="MPQFDILCKTPPKVLVRQFVERFERPSGEKIALCAAELTYLCWMI
                     THNGTAIKRATFMSYNTIISNSLSFDIVNKSLQFKYKTQKATILEASLKKLIPAWEFTI
                     IPYYGQKHQSDITDIVSSLQLQFESSEEADKGNSHSKKMLKALLSEGESIWEITEKILN
                     SFEYTSRFTKTKTLYQFLFLATFINCGRFSDIKNVDPKSFKLVQNKYLGVIIQCLVTET
                     KTSVSRHIYFFSARGRIDPLVYLDEFLRNSEPVLKRVNRTGNSSSNKQEYQLLKDNLVR
                     SYNKALKKNAPYSIFAIKNGPKSHIGRHLMTSFLSMKGLTELTNVVGNWSDKRASAVAR
                     TTYTHQITAIPDHYFALVSRYYAYDPISKEMIALKDETNPIEEWQHIEQLKGSAEGSIR
                     YPAWNGIISQEVLDYLSSYINRRI"
     protein_bind    3126..3173
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     promoter        6073..6288
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     CDS             6289..7092
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     CDS             join(7188..7256,7257..8048)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7406..7425)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8219..8807
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8708..8727
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8961..8978
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(9055..9302)
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     9268..9286
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     CDS             complement(join(10362..10373,10374..10571,10572..10628))
                     /codon_start=1
                     /gene="MF-alpha-1"
                     /product="N-terminal secretion signal from S. cerevisiae 
                     alpha-factor"
                     /label=alpha-factor secretion signal
                     /note="Cleavage by the Kex2 protease occurs after the 
                     dibasic KR sequence. The EA dipeptides are then removed by 
                     dipeptidyl aminopeptidase A."
                     /translation="MRFPSIFTAVLFAASSALAAPVNTTTEDETAQIPAEAVIGYSDLE
                     GDFDVAVLPFSNSTNNGLLFINTTIASIAAKEEGVSLEKREAEA"
     promoter        complement(10654..11095)
                     /gene="S. cerevisiae GAL1"
                     /label=GAL1 promoter
                     /note="inducible promoter, regulated by Gal4"
     primer_bind     complement(10660..10683)
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     protein_bind    10978..11095
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     rep_origin      11117..11572
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(11204..11223)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     11414..11435
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 gtgcgttttt ggttttttga aagtgcgtct tcagagcgct tttggttttc aaaagcgctc
       61 tgaagttcct atactttcta gagaatagga acttcggaat aggaacttca aagcgtttcc
      121 gaaaacgagc gcttccgaaa atgcaacgcg agctgcgcac atacagctca ctgttcacgt
      181 cgcacctata tctgcgtgtt gcctgtatat atatatacat gagaagaacg gcatagtgcg
      241 tgtttatgct taaatgcgta cttatatgcg tctatttatg taggatgaaa ggtagtctag
      301 tacctcctgt gatattatcc cattccatgc ggggtatcgt atgcttcctt cagcactacc
      361 ctttagctgt tctatatgct gccactcctc aattggatta gtctcatcct tcaatgctat
      421 catttccttt gatattggat cataccctag aagtattacg tgattttctg ccccttaccc
      481 tcgttgctac tctccttttt ttcgtgggaa ccgctttagg gccctcagtg atggtgtttt
      541 gtaatttata tgctcctctt gcatttgtgt ctctacttct tgttcgcctg gagggaactt
      601 cttcatttgt attagcatgg ttcacttcag tccttccttc caactcactc tttttttgct
      661 gtaaacgatt ctctgccgcc agttcattga aactattgaa tatatccttt agagattccg
      721 ggatgaataa atcacctatt aaagcagctt gacgatctgg tggaactaaa gtaagcaatt
      781 gggtaacgac gcttacgagc ttcataacat cttcttccgt tggagctggt gggactaata
      841 actgtgtaca atccattttt ctcatgagca tttcggtagc tctcttcttg tctttctcgg
      901 gcaatcttcc tattattata gcaatagatt tgtatagttg ctttctattg tctaacagct
      961 tgttattctg tagcatcaaa tctatggcag cctgacttgc ttcttgtgaa gagagcatac
     1021 catttccaat cgaatcaaac ctttccttaa ccatcttcgc agcaggcaaa attacctcag
     1081 cactggagtc agaagatacg ctggaatctt ctgcgctaga atcaagacca tacggcctac
     1141 cggttgtgag agattccatg ggccttatga catatcctgg aaagagtagc tcatcagact
     1201 tacgtttact ctctatatca atatctacat caggagcaat catttcaata aacagccgac
     1261 atacatccca gacgctataa gctgtacgtg cttttaccgt cagattcttg gctgtttcaa
     1321 tgtcgtccat tttggttttc ttttaccagt attgttcgtt tgataatgta ttcttgctta
     1381 ttacattata aaatctgtgc agatcacatg tcaaaacaac tttttatcac aagatagtac
     1441 cgcaaaacga acctgcgggc cgtctaaaaa ttaaggaaaa gcagcaaagg tgcattttta
     1501 aaatatgaaa tgaagatacc gcagtaccaa ttattttcgc agtacaaata atgcgcggcc
     1561 ggtgcatttt tcgaaagaac gcgagacaaa caggacaatt aaagttagtt tttcgagtta
     1621 gcgtgtttga atactgcaag atacaagata aatagagtag ttgaaactag atatcaattg
     1681 cacacaagat cggcgctaag catgccacaa tttgatatat tatgtaaaac accacctaag
     1741 gtgcttgttc gtcagtttgt ggaaaggttt gaaagacctt caggtgagaa aatagcatta
     1801 tgtgctgctg aactaaccta tttatgttgg atgattacac ataacggaac agcaatcaag
     1861 agagccacat tcatgagcta taatactatc ataagcaatt cgctgagttt cgatattgtc
     1921 aataaatcac tccagtttaa atacaagacg caaaaagcaa caattctgga agcctcatta
     1981 aagaaattga ttcctgcttg ggaatttaca attattcctt actatggaca aaaacatcaa
     2041 tctgatatca ctgatattgt aagtagtttg caattacagt tcgaatcatc ggaagaagca
     2101 gataagggaa atagccacag taaaaaaatg cttaaagcac ttctaagtga gggtgaaagc
     2161 atctgggaga tcactgagaa aatactaaat tcgtttgagt atacttcgag atttacaaaa
     2221 acaaaaactt tataccaatt cctcttccta gctactttca tcaattgtgg aagattcagc
     2281 gatattaaga acgttgatcc gaaatcattt aaattagtcc aaaataagta tctgggagta
     2341 ataatccagt gtttagtgac agagacaaag acaagcgtta gtaggcacat atacttcttt
     2401 agcgcaaggg gtaggatcga tccacttgta tatttggatg aatttttgag gaattctgaa
     2461 ccagtcctaa aacgagtaaa taggaccggc aattcttcaa gcaataaaca ggaataccaa
     2521 ttattaaaag ataacttagt cagatcgtac aataaagctt tgaagaaaaa tgcgccttat
     2581 tcaatctttg ctataaaaaa tggcccaaaa tctcacattg gaagacattt gatgacctca
     2641 tttctttcaa tgaagggcct aacggagttg actaatgttg tgggaaattg gagcgataag
     2701 cgtgcttctg ccgtggccag gacaacgtat actcatcaga taacagcaat acctgatcac
     2761 tacttcgcac tagtttctcg gtactatgca tatgatccaa tatcaaagga aatgatagca
     2821 ttgaaggatg agactaatcc aattgaggag tggcagcata tagaacagct aaagggtagt
     2881 gctgaaggaa gcatacgata ccccgcatgg aatgggataa tatcacagga ggtactagac
     2941 tacctttcat cctacataaa tagacgcata taagtacgca tttaagcata aacacgcact
     3001 atgccgttct tctcatgtat atatatatac aggcaacacg cagatatagg tgcgacgtga
     3061 acagtgagct gtatgtgcgc agctcgcgtt gcattttcgg aagcgctcgt tttcggaaac
     3121 gctttgaagt tcctattccg aagttcctat tctctagaaa gtataggaac ttcagagcgc
     3181 ttttgaaaac caaaagcgct ctgaagacgc actttcaaaa aaccaaaaac gcaccggact
     3241 gtaacgagct actaaaatat tgcgaatacc gcttccacaa acattgctca aaagtatctc
     3301 tttgctatat atctctgtgc tatatcccta tataacctac ccatccacct ttcgctcctt
     3361 gaacttgcat ctaaactcga cctctacatc aacaggcttc caatgctctt caaattttac
     3421 tgtcaagtag acccatacgg ctgtaatatg ctgctcttca taatgtaagc ttatctttat
     3481 cgaatcgtgt gaaaaactac taccgcgata aacctttacg gttccctgag attgaattag
     3541 ttcctttagt atatgataca agacactttt gaactttgta cgacgaattt tgaggttcgc
     3601 catcctctgg ctatttccaa ttatcctgtc ggctattatc tccgcctcag tttgatcttc
     3661 cgcttcagac tgccattttt cacataatga atctatttca ccccacaatc cttcatccgc
     3721 ctccgcatct tgttccgtta aactattgac ttcatgttgt acattgttta gttcacgaga
     3781 agggtcctct tcaggcggta gctcctgatc tcctatatga cctttatcct gttctctttc
     3841 cacaaactta gaaatgtatt catgaattat ggagcaccta ataacattct tcaaggcgga
     3901 gaagtttggg ccagatgccc aatatgcttg acatgaaaac gtgagaatga atttagtatt
     3961 attgtgatat tctgaggcaa ttttattata atctcgaaga taagagaaga atgcagtgac
     4021 ctttgtattg acaaatggag attccatgta tctaaaaaat acgcctttag gccttctgat
     4081 accctttccc ctgcggttta gcgtgccttt tacattaata tctaaaccct ctccgatggt
     4141 ggcctttaac tgactaataa atgcaaccga tataaactgt gataattctg ggtgatttat
     4201 gattcgatcg acaattgtat tgtacactag tgcaggatca ggccaatcca gttctttttc
     4261 aattaccggt gtgtcgtctg tattcagtac atgtccaaca aatgcaaatg ctaacgtttt
     4321 gtatttctta taattgtcag gaactggaaa agtccccctt gtcgtctcga ttacacacct
     4381 actttcatcg tacaccatag gttggaagtg ctgcataata cattgcttaa tacaagcaag
     4441 cagtctctcg ccattcatat ttcagttatt ttccattaca gctgatgtca ttgtatatca
     4501 gcgctgtaaa aatctatctg ttacagaagg ttttcgcggt ttttataaac aaaactttcg
     4561 ttacgaaatc gagcaatcac cccagctgcg tatttggaaa ttcgggaaaa agtagagcaa
     4621 cgcgagttgc attttttaca ccataatgca tgattaactt cgagaaggga ttaaggctaa
     4681 tttcactagt atgtttcaaa aacctcaatc tgtccattga atgccttata aaacagctat
     4741 agattgcata gaagagttag ctactcaatg ctttttgtca aagcttactg atgatgatgt
     4801 gtctactttc aggcgggtct gtagtaagga gaatgacgtt ataaagctgg cacttagaat
     4861 tccacggact atagactata ctagtatact ccgtctactg tacgatacac ttccgctcag
     4921 gtccttgtcc tttaacgagg ccttaccact cttttgttac tctattgatc cagctcagca
     4981 aaggcagtgt gatctaagat tctatcttcg cgatgtagta aaactagcta gatcgagaaa
     5041 gagactagaa atgcaaaagg cacttctaca atggctgcca tcattattat ccgatgtgac
     5101 gctgcagctt ctcaatgata ttcgaatacg ctttgaggag atacagccta atatccgaca
     5161 aactgtttta cagatttacg atcgtacttg ttacccatca ttgaattttg aacatccgaa
     5221 cctgggagtt ttccctgaaa cagatagtat atttgaacct gtataataat atatagtcta
     5281 gcgctttacg gaagacaatg tatgtatttc ggttcctgga gaaactattg catctattgc
     5341 ataggtaatc ttgcacgtcg catccccggt tcattttctg cgtttccatc ttgcacttca
     5401 atagcatatc tttgttaacg aagcatctgt gcttcatttt gtagaacaaa aatgcaacgc
     5461 gagagcgcta atttttcaaa caaagaatct gagctgcatt tttacagaac agaaatgcaa
     5521 cgcgaaagcg ctattttacc aacgaagaat ctgtgcttca tttttgtaaa acaaaaatgc
     5581 aacgcgagag cgctaatttt tcaaacaaag aatctgagct gcatttttac agaacagaaa
     5641 tgcaacgcga gagcgctatt ttaccaacaa agaatctata cttctttttt gttctacaaa
     5701 aatgcatccc gagagcgcta tttttctaac aaagcatctt agattacttt ttttctcctt
     5761 tgtgcgctct ataatgcagt ctcttgataa ctttttgcac tgtaggtccg ttaaggttag
     5821 aagaaggcta ctttggtgtc tattttctct tccataaaaa aagcctgact ccacttcccg
     5881 cgtttactga ttactagcga agctgcgggt gcattttttc aagataaagg catccccgat
     5941 tatattctat accgatgtgg attgcgcata ctttgtgaac agaaagtgat agcgttgatg
     6001 attcttcatt ggtcagaaaa ttatgaacgg tttcttctat tttgtctcta tatactacgt
     6061 acctgctagc ttttcaattc atcatttttt ttttattctt ttttttgatt tcggtttcct
     6121 tgaaattttt ttgattcggt aatctccgaa cagaaggaag aacgaaggaa ggagcacaga
     6181 cttagattgg tatatatacg catatgtagt gttgaagaaa catgaaattg cccagtattc
     6241 ttaacccaac tgcacagaac aaaaacctgc aggaaacgaa gataaatcat gtcgaaagct
     6301 acatataagg aacgtgctgc tactcatcct agtcctgttg ctgccaagct atttaatatc
     6361 atgcacgaaa agcaaacaaa cttgtgtgct tcattggatg ttcgtaccac caaggaatta
     6421 ctggagttag ttgaagcatt aggtcccaaa atttgtttac taaaaacaca tgtggatatc
     6481 ttgactgatt tttccatgga gggcacagtt aagccgctaa aggcattatc cgccaagtac
     6541 aattttttac tcttcgaaga cagaaaattt gctgacattg gtaatacagt caaattgcag
     6601 tactctgcgg gtgtatacag aatagcagaa tgggcagaca ttacgaatgc acacggtgtg
     6661 gtgggcccag gtattgttag cggtttgaag caggcggcag aagaagtaac aaaggaacct
     6721 agaggccttt tgatgttagc agaattgtca tgcaagggct ccctatctac tggagaatat
     6781 actaagggta ctgttgacat tgcgaagagc gacaaagatt ttgttatcgg ctttattgct
     6841 caaagagaca tgggtggaag agatgaaggt tacgattggt tgattatgac acccggtgtg
     6901 ggtttagatg acaagggaga cgcattgggt caacagtata gaaccgtgga tgatgtggtc
     6961 tctacaggat ctgacattat tattgttgga agaggactat ttgcaaaggg aagggatgct
     7021 aaggtagagg gtgaacgtta cagaaaagca ggctgggaag catatttgag aagatgcggc
     7081 cagcaaaact aaaaaactgt attataagta aatgcatgta tactaaactc acaaattaga
     7141 gcttcaattt aattatatca gttattaccc attgaaaaag gaagagtatg agtattcaac
     7201 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
     7261 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
     7321 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
     7381 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
     7441 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
     7501 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
     7561 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
     7621 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
     7681 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
     7741 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
     7801 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
     7861 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
     7921 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     7981 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     8041 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     8101 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     8161 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     8221 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     8281 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     8341 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
     8401 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     8461 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     8521 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     8581 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     8641 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     8701 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     8761 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     8821 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     8881 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
     8941 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac
     9001 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagggccgca gcttgcaaat
     9061 taaagccttc gagcgtccca aaaccttctc aagcaaggtt ttcagtataa tgttacatgc
     9121 gtacacgcgt ctgtacagaa aaaaaagaaa aatttgaaat ataaataacg ttcttaatac
     9181 taacataact ataaaaaaat aaatagggac ctagacttca ggttgtctaa ctccttcctt
     9241 ttcggttaga gcggatgtgg ggggagggcg tgaatgtaag cgtgacataa ctaattacat
     9301 gatgcggccc tctagatgca tgctcgagcg gccgcctagg caggtgggac tggtggaact
     9361 gatgttgggg gaccaggatc tgccggaagc gtgggaaaag gggcagtcgc gcaagcctgc
     9421 tccacatctg tctttgattt accggcgggc aaatgtggct tagtcgttaa tggtggtggg
     9481 acgggtatta catccgaaca gtctatcatg tcgtcttgat tctggcccaa catagacatt
     9541 ttgaaaagtg tgtctctgaa cctgtcctga atcttttgct gatcattaac cattgactgc
     9601 cattcacaag ctgtacgtgg atctctggca aataaatggt cgctctgcaa tctcatctcg
     9661 cctggtagtg gcgatttgac ctctccaggg ttaccgccag tgcccgggaa ggttgttcct
     9721 cttagctgag tttctatgaa gaactgcgag tcgaataaat taggagtgga gtcgaatggc
     9781 gtaccaggga tagtggggtc aacatgatcg gcagcggcaa ctgaatgcgc ggatagcaac
     9841 caaaccacct caaccggact gaaaccggcg tctgccatcc tcgcaagaat gtcggtaacg
     9901 gagtcgaatg gctccggaac caatcctggg ggcgatggtg ccgtggcagg gggcctacct
     9961 aagaagaagg gaatcctagg cgctccgggg cagtttgtta ctgctaaagt tgctgcaaaa
    10021 tggaccaggt ccccagccga gatgttgtgt ctctgaagaa atgacttctc tgcctcaacg
    10081 atatcatcaa tgccaaggtt ggcggggaag tttgtctcaa tatcactaaa cgtaatcaca
    10141 gaaccatccg cgccgcccat tacccctcta ctggggctga aacctattgc gtcatggaac
    10201 gtcaaacgta gtgcctcgtg agcttgctcg ccacattgac caccgtcaaa taggttcttt
    10261 tgaagatctt ccatcaaatc gaatagaacg caacaggcct cgttacttgt agtctggccg
    10321 ccggagcatg tggctctttt ctgtggtaaa gaaacgaatt cagcttcagc ctctcttttc
    10381 tcgagagata ccccttcttc tttagcagca atgctggcaa tagtagtatt tataaacaat
    10441 aacccgttat ttgtgctgtt ggaaaatggc aaaacagcaa catcgaaatc cccttctaaa
    10501 tctgagtaac cgatgacagc ttcagccgga atttgtgccg tttcatcttc tgttgtagtg
    10561 ttgactggag cagctaatgc ggaggatgct gcgaataaaa cagcagtaaa aattgaagga
    10621 aatctcatgg atcctatgat cctatagttt tttctccttg acgttaaagt atagaggtat
    10681 attaacaatt ttttgttgat acttttatta catttgaata agaagtaata caaaccgaaa
    10741 atgttgaaag tattagttaa agtggttatg cagtttttgc atttatatat ctgttaatag
    10801 atcaaaaatc atcgcttcgc tgattaatta ccccagaaat aaggctaaaa aactaatcgc
    10861 attatcatcc tatggttgtt aatttgattc gttcatttga aggtttgtgg ggccaggtta
    10921 ctgccaattt ttcctcttca taaccataaa agctagtatt gtagaatctt tattgttcgg
    10981 agcagtgcgg cgcgaggcac atctgcgttt caggaacgcg accggtgaag acgaggacgc
    11041 acggaggaga gtcttccttc ggagggctgt cacccgctcg gcggcttcta atccgtggac
    11101 tagtggatcg atccccacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
    11161 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc
    11221 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct
    11281 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat
    11341 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc
    11401 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc
    11461 tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg
    11521 atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat ttctctacgt
    11581 ataggaaatg tttacatttt cgtattgttt tcgattcact ctatgaatag ttcttactac
    11641 aatttttttg tctaaagagt aatactagag ataaacataa aaaatgtaga ggtcgagttt
    11701 agatgcaagt tcaaggagcg aaaggtggat gggtaggtta tatagggata tagcacagag
    11761 atatatagca aagagatact tttgagcaat gtttgtggaa gcggtattcg caatatttta
    11821 gtagctcgtt acagtccg
//
