LOCUS       sequence-370134-       12294 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  FuncLib-designed high-redox potential laccase from Trametes hirsuta 
            (Th3.1).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12294)
  AUTHORS   Barber-Zucker S, Mateljak I, Goldsmith M, Kupervaser M, Alcalde M, 
            Fleishman SJ
  TITLE     Designed High-Redox Potential Laccases Exhibit High Functional 
            Diversity.
  JOURNAL   ACS Catal. 2022 Nov 4;12(21):13164-13173. doi: 
            10.1021/acscatal.2c03006. Epub 2022 Oct 13.
  PUBMED    36366766
REFERENCE   2  (bases 1 to 12294)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acscatal.2c03006"; journalName: "ACS Catal"; date: 
            "2022-11-4- 4"; volume: "12"; issue: "21"; pages: "13164-13173"
COMMENT     Sequence Label: pJRoC30_Th3.1
FEATURES             Location/Qualifiers
     source          1..12294
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    2164..2211
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     promoter        5111..5326
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     CDS             5327..6130
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     CDS             join(6226..6294,6295..7086)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6444..6463)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7257..7845
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7746..7765
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7999..8016
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(8093..8340)
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     8306..8324
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     CDS             complement(8377..8394)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(8401..8418)
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             complement(join(9931..9942,9943..10140,10141..10197))
                     /codon_start=1
                     /gene="MF-alpha-1"
                     /product="N-terminal secretion signal from S. cerevisiae 
                     alpha-factor"
                     /label=alpha-factor secretion signal
                     /note="Cleavage by the Kex2 protease occurs after the 
                     dibasic KR sequence. The EA dipeptides are then removed by 
                     dipeptidyl aminopeptidase A."
                     /translation="MRFPSIFTADLFAASSALAAPVKTTTEDETAQIPAEAVIGYSDLE
                     GDFDVAVLPFSNSTNNGLLFINTTIASIAAKEEGVSLEKRETEA"
     promoter        complement(10224..10665)
                     /gene="S. cerevisiae GAL1"
                     /label=GAL1 promoter
                     /note="inducible promoter, regulated by Gal4"
     primer_bind     complement(10230..10253)
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     protein_bind    10548..10665
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     rep_origin      10687..11142
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10774..10793)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10984..11005
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     protein_bind    complement(11470..11517)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
ORIGIN
        1 cttgtctttc tcgggcaatc ttcctattat tatagcaata gatttgtata gttgctttct
       61 attgtctaac agcttgttat tctgtagcat caaatctatg gcagcctgac ttgcttcttg
      121 tgaagagagc ataccatttc caatcgaatc aaacctttcc ttaaccatct tcgcagcagg
      181 caaaattacc tcagcactgg agtcagaaga tacgctggaa tcttctgcgc tagaatcaag
      241 accatacggc ctaccggttg tgagagattc catgggcctt atgacatatc ctggaaagag
      301 tagctcatca gacttacgtt tactctctat atcaatatct acatcaggag caatcatttc
      361 aataaacagc cgacatacat cccagacgct ataagctgta cgtgctttta ccgtcagatt
      421 cttggctgtt tcaatgtcgt ccattttggt tttcttttac cagtattgtt cgtttgataa
      481 tgtattcttg cttattacat tataaaatct gtgcagatca catgtcaaaa caacttttta
      541 tcacaagata gtaccgcaaa acgaacctgc gggccgtcta aaaattaagg aaaagcagca
      601 aaggtgcatt tttaaaatat gaaatgaaga taccgcagta ccaattattt tcgcagtaca
      661 aataatgcgc ggccggtgca tttttcgaaa gaacgcgaga caaacaggac aattaaagtt
      721 agtttttcga gttagcgtgt ttgaatactg caagatacaa gataaataga gtagttgaaa
      781 ctagatatca attgcacaca agatcggcgc taagcatgcc acaatttgat atattatgta
      841 aaacaccacc taaggtgctt gttcgtcagt ttgtggaaag gtttgaaaga ccttcaggtg
      901 agaaaatagc attatgtgct gctgaactaa cctatttatg ttggatgatt acacataacg
      961 gaacagcaat caagagagcc acattcatga gctataatac tatcataagc aattcgctga
     1021 gtttcgatat tgtcaataaa tcactccagt ttaaatacaa gacgcaaaaa gcaacaattc
     1081 tggaagcctc attaaagaaa ttgattcctg cttgggaatt tacaattatt ccttactatg
     1141 gacaaaaaca tcaatctgat atcactgata ttgtaagtag tttgcaatta cagttcgaat
     1201 catcggaaga agcagataag ggaaatagcc acagtaaaaa aatgcttaaa gcacttctaa
     1261 gtgagggtga aagcatctgg gagatcactg agaaaatact aaattcgttt gagtatactt
     1321 cgagatttac aaaaacaaaa actttatacc aattcctctt cctagctact ttcatcaatt
     1381 gtggaagatt cagcgatatt aagaacgttg atccgaawts rywyawatwm ktcywwaryr
     1441 mrwrkskkrn ngkawyratc casttgtwta ttwsrawgaa wwwkwsargm rttctgaacc
     1501 agtcctaaaa cgagtaaata ggaccggcaa ttcttcaagc aataaacagg aataccaatt
     1561 attaaaagat aacttagtca gatcgtacaa taaagctttg aagaaaaatg cgccttattc
     1621 aatctttgct ataaaaaatg gcccaaaatc tcacattgga agacatttga tgacctcatt
     1681 tctttcaatg aagggcctaa cggagttgac taatgttgtg ggaaattgga gcgataagcg
     1741 tgcttctgcc gtggccagga caacgtatac tcatcagata acagcaatac ctgatcacta
     1801 cttcgcacta gtttctcggt actatgcata tgatccaata tcaaaggaaa tgatagcatt
     1861 gaaggatgag actaatccaa ttgaggagtg gcagcatata gaacagctaa agggtagtgc
     1921 tgaaggaagc atacgatacc ccgcatggaa tgggataata tcacaggagg tactagacta
     1981 cctttcatcc tacataaata gacgcatata agtacgcatt taagcataaa cacgcactat
     2041 gccgttcttc tcatgtatat atatatacag gcaacacgca gatataggtg cgacgtgaac
     2101 agtgagctgt atgtgcgcag ctcgcgttgc attttcggaa gcgctcgttt tcggaaacgc
     2161 tttgaagttc ctattccgaa gttcctattc tctagaaagt ataggaactt cagagcgctt
     2221 ttgaaaacca aaagcgctct gaagacgcac tttcaaaaaa ccaaaaacgc accggactgt
     2281 aacgagctac taaaatattg cgaataccgc ttccacaaac attgctcaaa agtatctctt
     2341 tgctatatat ctctgtgcta tatccctata taacctaccc atccaccttt cgctccttga
     2401 acttgcatct aaactcgacc tctacatcaa caggcttcca atgctcttca aattttactg
     2461 tcaagtagac ccatacggct gtaatatgct gctcttcata atgtaagctt atctttatcg
     2521 aatcgtgtga aaaactacta ccgcgataaa cctttacggt tccctgagat tgaattagtt
     2581 cctttagtat atgatacaag acacttttga actttgtacg acgaattttg aggttcgcca
     2641 tcctctggct atttccaatt atcctgtcgg ctattatctc cgcctcagtt tgatcttccg
     2701 cttcagactg ccatttttca cataatgaat ctatttcacc ccacaatcct tcatccgcct
     2761 ccgcatcttg ttccgttaaa ctattgactt catgttgtac attgtttagt tcacgagaag
     2821 ggtcctcttc aggcggtagc tcctgatctc ctatatgacc tttatcctgt tctctttcca
     2881 caaacttaga aatgtattca tgaattatgg agcacctaat aacattcttc aaggcggaga
     2941 agtttgggcc agatgcccaa tatgcttgac atgaaaacgt gagaatgaat ttagtattat
     3001 tgtgatattc tgaggcaatt ttattataat ctcgaagata agagaagaat gcagtgacct
     3061 ttgtattgac aaatggagat tccatgtatc taaaaaatac gcctttaggc cttctgatac
     3121 cctttcccct gcggtttagc gtgcctttta cattaatatc taaaccctct ccgatggtgg
     3181 cctttaactg actaataaat gcaaccgata taaactgtga taattctggg tgatttatga
     3241 ttcgatcgac aattgtattg tacactagtg caggatcagg ccaatccagt tctttttcaa
     3301 ttaccggtgt gtcgtctgta ttcagtacat gtccaacaaa tgcaaatgct aacgttttgt
     3361 atttcttata attgtcagga actggaaaag tcccccttgt cgtctcgatt acacacctac
     3421 tttcatcgta caccataggt tggaagtgct gcataataca ttgcttaata caagcaagca
     3481 gtctctcgcc attcatattt cagttatttt ccattacagc tgatgtcatt gtatatcagc
     3541 gctgtaaaaa tctatctgtt acagaaggtt ttcgcggttt ttataaacaa aactttcgtt
     3601 acgaaatcga gcaatcaccc cagctgcgta tttggaaatt cgggaaaaag tagagcaacg
     3661 cgagttgcat tttttacacc ataatgcatg attaacttcg agaagggatt aaggctaatt
     3721 tcactagtat gtttcaaaaa cctcaatctg tccattgaat gccttataaa acagctatag
     3781 attgcataga agagttagct actcaatgct ttttgtcaaa gcttactgat gatgatgtgt
     3841 ctactttcag gcgggtctgt agtaaggaga atgacattat aaagctggca cttagaattc
     3901 cacggactat agactatact agtatactcc gtctactgta cgatacactt ccgctcaggt
     3961 ccttgtcctt taacgaggcc ttaccactct tttgttactc tattgatcca gctcagcaaa
     4021 ggcagtgtga tctaagattc tatcttcgcg atgtagtaaa actagctaga ccgagaaaga
     4081 gactagaaat gcaaaaggca cttctacaat ggctgccatc attattatcc gatgtgacgc
     4141 tgcagcttct caatgatatt cgaatacgct ttgaggagat acagcctaat atccgacaaa
     4201 ctgttttaca gatttacgat cgtacttgtt acccatcatt gaattttgaa catccgaacc
     4261 tgggagtttt ccctgaaaca gatagtatat ttgaacctgt ataataatat atagtctagc
     4321 gctttacgga agacaatgta tgtatttcgg ttcctggaga aactattgca tctattgcat
     4381 aggtaatctt gcacgtcgca tccccggttc attttctgcg tttccatctt gcacttcaat
     4441 agcatatctt tgttaacgaa gcatctgtgc ttcattttgt agaacaaaaa tgcaacgcga
     4501 gagcgctaat ttttcaaaca aagaatctga gctgcatttt tacagaacag aaatgcaacg
     4561 cgaaagcgct attttaccaa cgaagaatct gtgcttcatt tttgtaaaac aaaaatgcaa
     4621 cgcgagagcg ctaatttttc aaacaaagaa tctgagctgc atttttacag aacagaaatg
     4681 caacgcgaga gcgctatttt accaacaaag aatctatact tcttttttgt tctacaaaaa
     4741 tgcatcccga gagcgctatt tttctaacaa agcatcttag attacttttt ttctcctttg
     4801 tgcgctctat aatgcagtct cttgataact ttttgcactg taggtccgtt aaggttagaa
     4861 gaaggctact ttggtgtcta ttttctcttc cataaaaaaa gcctgactcc acttcccgcg
     4921 tttactgatt actagcgaag ctgcgggtgc attttttcaa gataaaggca tccccgatta
     4981 tattctatac cgatgtggat tgcgcatact ttgtgaacag aaagtgatag cgttgatgat
     5041 tcttcattgg tcagaaaatt atgaacggtt tcttctattt tgtctctata tactacgtac
     5101 ctgctagctt ttcaattcat catttttttt ttattctttt ttttgatttc ggtttccttg
     5161 aaattttttt gattcggtaa tctccgaaca gaaggaagaa cgaaggaagg agcacagact
     5221 tagattggta tatatacgca tatgtagtgt tgaagaaaca tgaaattgcc cagtattctt
     5281 aacccaactg cacagaacaa aaacctgcag gaaacgaaga taaatcatgt cgaaagctac
     5341 atataaggaa cgtgctgcta ctcatcctag tcctgttgct gccaagctat ttaatatcat
     5401 gcacgaaaag caaacaaact tgtgtgcttc attggatgtt cgtaccacca aggaattact
     5461 ggagttagtt gaagcattag gtcccaaaat ttgtttacta aaaacacatg tggatatctt
     5521 gactgatttt tccatggagg gcacagttaa gccgctaaag gcattatccg ccaagtacaa
     5581 ttttttactc ttcgaagaca gaaaatttgc tgacattggt aatacagtca aattgcagta
     5641 ctctgcgggt gtatacagaa tagcagaatg ggcagacatt acgaatgcac acggtgtggt
     5701 gggcccaggt attgttagcg gtttgaagca ggcggcagaa gaagtaacaa aggaacctag
     5761 aggccttttg atgttagcag aattgtcatg caagggctcc ctatctactg gagaatatac
     5821 taagggtact gttgacattg cgaagagcga caaagatttt gttatcggct ttattgctca
     5881 aagagacatg ggtggaagag atgaaggtta cgattggttg attatgacac ccggtgtggg
     5941 tttagatgac aagggagacg cattgggtca acagtataga accgtggatg atgtggtctc
     6001 tacaggatct gacattatta ttgttggaag aggactattt gcaaagggaa gggatgctaa
     6061 ggtagagggt gaacgttaca gaaaagcagg ctgggaagca tatttgagaa gatgcggcca
     6121 gcaaaactaa aaaactgtat tataagtaaa tgcatgtata ctaaactcac aaattagagc
     6181 ttcaatttaa ttatatcagt tattacccat tgaaaaagga agagtatgag tattcaacat
     6241 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
     6301 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc
     6361 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca
     6421 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg
     6481 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca
     6541 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata
     6601 accatgagtg ataacactgc ggccaactta ctcctgacaa cgatcggagg accgaaggag
     6661 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg
     6721 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca
     6781 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta
     6841 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct
     6901 ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca
     6961 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag
     7021 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
     7081 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
     7141 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
     7201 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     7261 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     7321 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     7381 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
     7441 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     7501 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     7561 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     7621 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     7681 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     7741 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     7801 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     7861 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     7921 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
     7981 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc
     8041 aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gggccgcagc ttgcaaatta
     8101 aagccttcga gcgtcccaaa accttctcaa gcaaggtttt cagtataatg ttacatgcgt
     8161 acacgcgtct gtacagaaaa aaaagaaaaa tttgaaatat aaataacgtt cttaatacta
     8221 acataactat aaaaaaataa atagggacct agacttcagg ttgtctaact ccttcctttt
     8281 cggttagagc ggatgtgggg ggagggcgtg aatgtaagcg tgacataact aattacatga
     8341 tgcggccctc tagatgcatg ctcgagcggc cgcttagtgg tggtggtggt ggtgcgcgct
     8401 gctgccgcgg ggaactaaac cgctgctctg atcgtttggg tccaaggcat cgtaagtcgg
     8461 gcataagtca ctccaggcct gagggactgg gttcacagcc tttacgtcgg gcaggtcctc
     8521 ggccattact actgcgaaac cggcttccaa gtgccagtcg atgtgacagt gcaagaacca
     8581 aggccctggg ttgttagtgt cgaaacggat tgtaacgttg tcacccgccg caggtgtgcc
     8641 ggtatttacc acatccctga aaatagggtt gtcatagtta taaacagtag agcctgcaga
     8701 tctcacgacc gcaaacgtgt gtccatgtaa gtgaaagggg tgaggtgaac caggtgcagc
     8761 cgcagttgca ggaaaagata tctctattgt tgcattagat ggtagcacat acacgcttcc
     8821 actaggcaac aagtcctgcg cagtctgggc tccactcaat atttgtagta agacagggac
     8881 ggtaggtggg acaaaactaa ctccgttgat gaagaagttg ctaccgttga aattaaatgc
     8941 catatttatt gccttgtcta ctccgccagg agaggggcta ccaggcactg gagtgggaac
     9001 taaggggtgc aagtcagttt cgttcagcgg ctttacagat gtggtctgat ttgtggtagg
     9061 ctccacggca ggcgcgccgt cataacgaag aatagctgag tttatgccac cgtcgaaacc
     9121 gacgttgccc atattagggt tcgccctgat ccaatagttg tccaccggtt ggttagcgtc
     9181 cagcacgaaa ctgtatcttt gtgcggcgaa aatttgtatt gaatctactt caaggggttg
     9241 cacatttaca gagtcaactt cgattatggt taagttgtga ccgtctattg agaaagtgaa
     9301 gttcgggttg catgacagtg agacaagtct gaatctgtac cttttgcctt tagtgacctt
     9361 gattacggat aattcagcgg tggggtctga aggcgctcta cctttcccat tgattagcgt
     9421 cgcgtcagca cctggcggga acctgggtcc caactttgca gccgtgtggt accagtccgc
     9481 cagtgttatt actgtgctgt cattatctac atcatacata ctagcgtgtg ggtcgtttgg
     9541 gtcatacaca acaaaagggc cccttaatcc atcgcagtac tgcgtgctta gatgagaatg
     9601 ataccaaaag gtacccgctt ggtcaggtac ttggaaatca tatagaaatg aatgccccgg
     9661 tgctattggg cattggttta tgaaagctgg gccgtcggcc caattcgttc cgtgctggaa
     9721 gaatccgtgc cagtgaatag aagtagattt tagcatggtg tgatttgtaa ggttgtttat
     9781 tacatttaac tggaatctgt cgcccttgtt gccggcgatt agtggtccgg gcgtcacgcc
     9841 gttgaccacg actgcttgcc ttgagaaacc gtcagggctc actacggcat ctgtgatggt
     9901 tagatctgcg acggggccta ccgcgaactc agcttcagtc tctcttttct cgagagatac
     9961 cccttcttct ttagcagcaa tgctggcaat agtagtattt ataaacaata atccgttatt
    10021 tgtgctgttg gaaaatggca aaacagcaac atcgaaatcc ccttctaaat ctgagtaacc
    10081 gatgacagct tcagccggaa tttgtgccgt ttcatcttct gttgtagttt tgactggagc
    10141 agctaatgcg gaggatgctg cgaataaatc agcagtaaaa attgaaggaa atctcatgga
    10201 tcctatggat cctatagttt tttctccttg acgttaaagt atagaggtat attaacaatt
    10261 ttttgttgat acttttatta catttgaata agaagtaata caaaccgaaa atgttgaaag
    10321 tattagttaa agtggttatg cagtttttgc atttatatat ctgttaatag atcaaaaatc
    10381 atcgcttcgc tgattaatta ccccagaaat aaggctaaaa aactaatcgc attatcatcc
    10441 tatggttgtt aatttgattc gttcatttga aggtttgtgg ggccaggtta ctgccaattt
    10501 ttcctcttca taaccataaa agctagtatt gtagaatctt tattgttcgg agcagtgcgg
    10561 cgcgaggcac atctgcgttt caggaacgcg accggtgaag acgaggacgc acggaggaga
    10621 gtcttccttc ggagggctgt cacccgctcg gcggcttcta atccgtggac tagtggatcg
    10681 atccccacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
    10741 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc
    10801 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
    10861 gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta
    10921 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta
    10981 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg
    11041 atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa
    11101 aatttaacgc gaattttaac aaaatattaa cgcttacaat ttctctacgt ataggaaatg
    11161 tttacatttt cgtattgttt tcgattcact ctatgaatag ttcttactac aatttttttg
    11221 tctaaagagt aatactagag ataaacataa aaaatgtaga ggtcgagttt agatgcaagt
    11281 tcaaggagcg aaaggtggat gggtaggtta tatagggata tagcacagag atatatagca
    11341 aagagatact tttgagcaat gtttgtggaa gcggtattcg caatatttta gtagctcgtt
    11401 acagtccggt gcgtttttgg ttttttgaaa gtgcgtcttc agagcgcttt tggttttcaa
    11461 aagcgctctg aagttcctat actttctaga gaataggaac ttcggaatag gaacttcaaa
    11521 gcgtttccga aaacgagcgc ttccgaaaat gcaacgcgag ctgcgcacat acagctcact
    11581 gttcacgtcg cacctatatc tgcgtgttgc ctgtatatat atatacatga gaagaacggc
    11641 atagtgcgtg tttatgctta aatgcgtact tatatgcgtc tatttatgta ggatgaaagg
    11701 tagtctagta cctcctgtga tattatccca ttccatgcgg ggtatcgtat gcttccttca
    11761 gcactaccct ttagctgttc tatatgctgc cactcctcaa ttggattagt ctcatccttc
    11821 aatgctatca tttcctttga tattggatca taccctagaa gtattacgtg attttctgcc
    11881 ccttaccctc gttgctactc tccttttttt cgtgggaacc gctttagggc cctcagtgat
    11941 ggtgttttgt aatttatatg ctcctcttgc atttgtgtct ctacttcttg ttcgcctgga
    12001 gggaacttct tcatttgtat tagcatggtt cacttcagtc cttccttcca actcactctt
    12061 tttttgctgt aaacgattct ctgccgccag ttcattgaaa ctattgaata tatcctttag
    12121 agattccggg atgaataaat cacctattaa agcagcttga cgatctggtg gaactaaagt
    12181 aagcaattgg gtaacgacgc ttacgagctt cataacatct tcttccgttg gagctggtgg
    12241 gactaataac tgtgtacaat ccatttttct catgagcatt tcggtagctc tctt
//
