LOCUS       sequence-370135-       12371 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  FuncLib-designed high-redox potential laccase from Trametes hirsuta 
            (Th3.10).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12371)
  AUTHORS   Barber-Zucker S, Mateljak I, Goldsmith M, Kupervaser M, Alcalde M, 
            Fleishman SJ
  TITLE     Designed High-Redox Potential Laccases Exhibit High Functional 
            Diversity.
  JOURNAL   ACS Catal. 2022 Nov 4;12(21):13164-13173. doi: 
            10.1021/acscatal.2c03006. Epub 2022 Oct 13.
  PUBMED    36366766
REFERENCE   2  (bases 1 to 12371)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acscatal.2c03006"; journalName: "ACS Catal"; date: 
            "2022-11-4- 4"; volume: "12"; issue: "21"; pages: "13164-13173"
COMMENT     Sequence Label: pJRoC30_Th3.10
FEATURES             Location/Qualifiers
     source          1..12371
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..456
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(88..107)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     298..319
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     protein_bind    complement(784..831)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             2424..3695
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=FLP
                     /note="FLP is a site-specific recombinase from 
                     Saccharomyces cerevisiae. Recombination occurs at FRT 
                     sequences."
                     /translation="MPQFDILCKTPPKVLVRQFVERFERPSGEKIALCAAELTYLCWMI
                     THNGTAIKRATFMSYNTIISNSLSFDIVNKSLQFKYKTQKATILEASLKKLIPAWEFTI
                     IPYYGQKHQSDITDIVSSLQLQFESSEEADKGNSHSKKMLKALLSEGESIWEITEKILN
                     SFEYTSRFTKTKTLYQFLFLATFINCGRFSDIKNVDPKSFKLVQNKYLGVIIQCLVTET
                     KTSVSRHIYFFSARGRIDPLVYLDEFLRNSEPVLKRVNRTGNSSSNKQEYQLLKDNLVR
                     SYNKALKKNAPYSIFAIKNGPKSHIGRHLMTSFLSMKGLTELTNVVGNWSDKRASAVAR
                     TTYTHQITAIPDHYFALVSRYYAYDPISKEMIALKDETNPIEEWQHIEQLKGSAEGSIR
                     YPAWNGIISQEVLDYLSSYINRRI"
     protein_bind    3848..3895
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     promoter        6795..7010
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     CDS             7011..7814
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     CDS             join(7910..7978,7979..8770)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8128..8147)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8941..9529
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9430..9449
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9683..9700
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(9777..10024)
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     9990..10008
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     CDS             complement(10062..10079)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(10086..10103)
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             complement(join(11616..11627,11628..11825,11826..11882))
                     /codon_start=1
                     /gene="MF-alpha-1"
                     /product="N-terminal secretion signal from S. cerevisiae 
                     alpha-factor"
                     /label=alpha-factor secretion signal
                     /note="Cleavage by the Kex2 protease occurs after the 
                     dibasic KR sequence. The EA dipeptides are then removed by 
                     dipeptidyl aminopeptidase A."
                     /translation="MRFPSIFTADLFAASSALAAPVKTTTEDETAQIPAEAVIGYSDLE
                     GDFDVAVLPFSNSTNNGLLFINTTIASIAAKEEGVSLEKRETEA"
     promoter        complement(11909..12350)
                     /gene="S. cerevisiae GAL1"
                     /label=GAL1 promoter
                     /note="inducible promoter, regulated by Gal4"
     primer_bind     complement(11915..11938)
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     protein_bind    12233..12350
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
ORIGIN
        1 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
       61 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
      121 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
      181 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
      241 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
      301 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
      361 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
      421 acgcgaattt taacaaaata ttaacgctta caatttctct acgtatagga aatgtttaca
      481 ttttcgtatt gttttcgatt cactctatga atagttctta ctacaatttt tttgtctaaa
      541 gagtaatact agagataaac ataaaaaatg tagaggtcga gtttagatgc aagttcaagg
      601 agcgaaaggt ggatgggtag gttatatagg gatatagcac agagatatat agcaaagaga
      661 tacttttgag caatgtttgt ggaagcggta ttcgcaatat tttagtagct cgttacagtc
      721 cggtgcgttt ttggtttttt gaaagtgcgt cttcagagcg cttttggttt tcaaaagcgc
      781 tctgaagttc ctatactttc tagagaatag gaacttcgga ataggaactt caaagcgttt
      841 ccgaaaacga gcgcttccga aaatgcaacg cgagctgcgc acatacagct cactgttcac
      901 gtcgcaccta tatctgcgtg ttgcctgtat atatatatac atgagaagaa cggcatagtg
      961 cgtgtttatg cttaaatgcg tacttatatg cgtctattta tgtaggatga aaggtagtct
     1021 agtacctcct gtgatattat cccattccat gcggggtatc gtatgcttcc ttcagcacta
     1081 ccctttagct gttctatatg ctgccactcc tcaattggat tagtctcatc cttcaatgct
     1141 atcatttcct ttgatattgg atcataccct agaagtatta cgtgattttc tgccccttac
     1201 cctcgttgct actctccttt ttttcgtggg aaccgcttta gggccctcag tgatggtgtt
     1261 ttgtaattta tatgctcctc ttgcatttgt gtctctactt cttgttcgcc tggagggaac
     1321 ttcttcattt gtattagcat ggttcacttc agtccttcct tccaactcac tctttttttg
     1381 ctgtaaacga ttctctgccg ccagttcatt gaaactattg aatatatcct ttagagattc
     1441 cgggatgaat aaatcaccta ttaaagcagc ttgacgatct ggtggaacta aagtaagcaa
     1501 ttgggtaacg acgcttacga gcttcataac atcttcttcc gttggagctg gtgggactaa
     1561 taactgtgta caatccattt ttctcatgag catttcggta gctctcttct tgtctttctc
     1621 gggcaatctt cctattatta tagcaataga tttgtatagt tgctttctat tgtctaacag
     1681 cttgttattc tgtagcatca aatctatggc agcctgactt gcttcttgtg aagagagcat
     1741 accatttcca atcgaatcaa acctttcctt aaccatcttc gcagcaggca aaattacctc
     1801 agcactggag tcagaagata cgctggaatc ttctgcgcta gaatcaagac catacggcct
     1861 accggttgtg agagattcca tgggccttat gacatatcct ggaaagagta gctcatcaga
     1921 cttacgttta ctctctatat caatatctac atcaggagca atcatttcaa taaacagccg
     1981 acatacatcc cagacgctat aagctgtacg tgcttttacc gtcagattct tggctgtttc
     2041 aatgtcgtcc attttggttt tcttttacca gtattgttcg tttgataatg tattcttgct
     2101 tattacatta taaaatctgt gcagatcaca tgtcaaaaca actttttatc acaagatagt
     2161 accgcaaaac gaacctgcgg gccgtctaaa aattaaggaa aagcagcaaa ggtgcatttt
     2221 taaaatatga aatgaagata ccgcagtacc aattattttc gcagtacaaa taatgcgcgg
     2281 ccggtgcatt tttcgaaaga acgcgagaca aacaggacaa ttaaagttag tttttcgagt
     2341 tagcgtgttt gaatactgca agatacaaga taaatagagt agttgaaact agatatcaat
     2401 tgcacacaag atcggcgcta agcatgccac aatttgatat attatgtaaa acaccaccta
     2461 aggtgcttgt tcgtcagttt gtggaaaggt ttgaaagacc ttcaggtgag aaaatagcat
     2521 tatgtgctgc tgaactaacc tatttatgtt ggatgattac acataacgga acagcaatca
     2581 agagagccac attcatgagc tataatacta tcataagcaa ttcgctgagt ttcgatattg
     2641 tcaataaatc actccagttt aaatacaaga cgcaaaaagc aacaattctg gaagcctcat
     2701 taaagaaatt gattcctgct tgggaattta caattattcc ttactatgga caaaaacatc
     2761 aatctgatat cactgatatt gtaagtagtt tgcaattaca gttcgaatca tcggaagaag
     2821 cagataaggg aaatagccac agtaaaaaaa tgcttaaagc acttctaagt gagggtgaaa
     2881 gcatctggga gatcactgag aaaatactaa attcgtttga gtatacttcg agatttacaa
     2941 aaacaaaaac tttataccaa ttcctcttcc tagctacttt catcaattgt ggaagattca
     3001 gcgatattaa gaacgttgat ccgaaatcat ttaaattagt ccaaaataag tatctgggag
     3061 taataatcca gtgtttagtg acagagacaa agacaagcgt tagtaggcac atatacttct
     3121 ttagcgcaag gggtaggatc gatccacttg tatatttgga tgaatttttg aggaattctg
     3181 aaccagtcct aaaacgagta aataggaccg gcaattcttc aagcaataaa caggaatacc
     3241 aattattaaa agataactta gtcagatcgt acaataaagc tttgaagaaa aatgcgcctt
     3301 attcaatctt tgctataaaa aatggcccaa aatctcacat tggaagacat ttgatgacct
     3361 catttctttc aatgaagggc ctaacggagt tgactaatgt tgtgggaaat tggagcgata
     3421 agcgtgcttc tgccgtggcc aggacaacgt atactcatca gataacagca atacctgatc
     3481 actacttcgc actagtttct cggtactatg catatgatcc aatatcaaag gaaatgatag
     3541 cattgaagga tgagactaat ccaattgagg agtggcagca tatagaacag ctaaagggta
     3601 gtgctgaagg aagcatacga taccccgcat ggaatgggat aatatcacag gaggtactag
     3661 actacctttc atcctacata aatagacgca tataagtacg catttaagca taaacacgca
     3721 ctatgccgtt cttctcatgt atatatatat acaggcaaca cgcagatata ggtgcgacgt
     3781 gaacagtgag ctgtatgtgc gcagctcgcg ttgcattttc ggaagcgctc gttttcggaa
     3841 acgctttgaa gttcctattc cgaagttcct attctctaga aagtatagga acttcagagc
     3901 gcttttgaaa accaaaagcg ctctgaagac gcactttcaa aaaaccaaaa acgcaccgga
     3961 ctgtaacgag ctactaaaat attgcgaata ccgcttccac aaacattgct caaaagtatc
     4021 tctttgctat atatctctgt gctatatccc tatataacct acccatccac ctttcgctcc
     4081 ttgaacttgc atctaaactc gacctctaca tcaacaggct tccaatgctc ttcaaatttt
     4141 actgtcaagt agacccatac ggctgtaata tgctgctctt cataatgtaa gcttatcttt
     4201 atcgaatcgt gtgaaaaact actaccgcga taaaccttta cggttccctg agattgaatt
     4261 agttccttta gtatatgata caagacactt ttgaactttg tacgacgaat tttgaggttc
     4321 gccatcctct ggctatttcc aattatcctg tcggctatta tctccgcctc agtttgatct
     4381 tccgcttcag actgccattt ttcacataat gaatctattt caccccacaa tccttcatcc
     4441 gcctccgcat cttgttccgt taaactattg acttcatgtt gtacattgtt tagttcacga
     4501 gaagggtcct cttcaggcgg tagctcctga tctcctatat gacctttatc ctgttctctt
     4561 tccacaaact tagaaatgta ttcatgaatt atggagcacc taataacatt cttcaaggcg
     4621 gagaagtttg ggccagatgc ccaatatgct tgacatgaaa acgtgagaat gaatttagta
     4681 ttattgtgat attctgaggc aattttatta taatctcgaa gataagagaa gaatgcagtg
     4741 acctttgtat tgacaaatgg agattccatg tatctaaaaa atacgccttt aggccttctg
     4801 ataccctttc ccctgcggtt tagcgtgcct tttacattaa tatctaaacc ctctccgatg
     4861 gtggccttta actgactaat aaatgcaacc gatataaact gtgataattc tgggtgattt
     4921 atgattcgat cgacaattgt attgtacact agtgcaggat caggccaatc cagttctttt
     4981 tcaattaccg gtgtgtcgtc tgtattcagt acatgtccaa caaatgcaaa tgctaacgtt
     5041 ttgtatttct tataattgtc aggaactgga aaagtccccc ttgtcgtctc gattacacac
     5101 ctactttcat cgtacaccat aggttggaag tgctgcataa tacattgctt aatacaagca
     5161 agcagtctct cgccattcat atttcagtta ttttccatta cagctgatgt cattgtatat
     5221 cagcgctgta aaaatctatc tgttacagaa ggttttcgcg gtttttataa acaaaacttt
     5281 cgttacgaaa tcgagcaatc accccagctg cgtatttgga aattcgggaa aaagtagagc
     5341 aacgcgagtt gcatttttta caccataatg catgattaac ttcgagaagg gattaaggct
     5401 aatttcacta gtatgtttca aaaacctcaa tctgtccatt gaatgcctta taaaacagct
     5461 atagattgca tagaagagtt agctactcaa tgctttttgt caaagcttac tgatgatgat
     5521 gtgtctactt tcaggcgggt ctgtagtaag gagaatgaca ttataaagct ggcacttaaa
     5581 attccacgga ctatagacta tactagtata ctccgtctac tgtacgatac acttccgctc
     5641 aggtccttgt cctttaacga ggccttacca ctcttttgtt actctattga tccagctcag
     5701 caaaggcagt gtgatctaag attctatctt cgcgatgtag taaaactagc tagaccgaga
     5761 aagagactag aaatgcaaaa ggcacttcta caatggctgc catcattatt atccgatgtg
     5821 acgctgcagc ttctcaatga tattcgaata cgctttgagg agatacagcc taatatccga
     5881 caaactgttt tacagattta cgatcgtact tgttacccat cattgaattt tgaacatccg
     5941 aacctgggag ttttccctga aacagatagt atatttgaac ctgtataata atatatagtc
     6001 tagcgcttta cggaagacaa tgtatgtatt tcggttcctg gagaaactat tgcatctatt
     6061 gcataggtaa tcttgcacgt cgcatccccg gttcattttc tgcgtttcca tcttgcactt
     6121 caatagcata tctttgttaa cgaagcatct gtgcttcatt ttgtagaaca aaaatgcaac
     6181 gcgagagcgc taatttttca aacaaagaat ctgagctgca tttttacaga acagaaatgc
     6241 aacgcgaaag cgctatttta ccaacgaaga atctgtgctt catttttgta aaacaaaaat
     6301 gcaacgcgag agcgctaatt tttcaaacaa agaatctgag ctgcattttt acagaacaga
     6361 aatgcaacgc gagagcgcta ttttaccaac aaagaatcta tacttctttt ttgttctaca
     6421 aaaatgcatc ccgagagcgc tatttttcta acaaagcatc ttagattact ttttttctcc
     6481 tttgtgcgct ctataatgca gtctcttgat aactttttgc actgtaggtc cgttaaggtt
     6541 agaagaaggc tactttggtg tctattttct cttccataaa aaaagcctga ctccacttcc
     6601 cgcgtttact gattactagc gaagctgcgg gtgcattttt tcaagataaa ggcatccccg
     6661 attatattct ataccgatgt ggattgcgca tactttgtga acagaaagtg atagcgttga
     6721 tgattcttca ttggtcagaa aattatgaac ggtttcttct attttgtctc tatatactac
     6781 gtacctgcta gcttttcaat tcatcatttt ttttttattc ttttttttga tttcggtttc
     6841 cttgaaattt ttttgattcg gtaatctccg aacagaagga agaacgaagg aaggagcaca
     6901 gacttagatt ggtatatata cgcatatgta gtgttgaaga aacatgaaat tgcccagtat
     6961 tcttaaccca actgcacaga acaaaaacct gcaggaaacg aagataaatc atgtcgaaag
     7021 ctacatataa ggaacgtgct gctactcatc ctagtcctgt tgctgccaag ctatttaata
     7081 tcatgcacga aaagcaaaca aacttgtgtg cttcattgga tgttcgtacc accaaggaat
     7141 tactggagtt agttgaagca ttaggtccca aaatttgttt actaaaaaca catgtggata
     7201 tcttgactga tttttccatg gagggcacag ttaagccgct aaaggcatta tccgccaagt
     7261 acaatttttt actcttcgaa gacagaaaat ttgctgacat tggtaataca gtcaaattgc
     7321 agtactctgc gggtgtatac agaatagcag aatgggcaga cattacgaat gcacacggtg
     7381 tggtgggccc aggtattgtt agcggtttga agcaggcggc agaagaagta acaaaggaac
     7441 ctagaggcct tttgatgtta gcagaattgt catgcaaggg ctccctatct actggagaat
     7501 atactaaggg tactgttgac attgcgaaga gcgacaaaga ttttgttatc ggctttattg
     7561 ctcaaagaga catgggtgga agagatgaag gttacgattg gttgattatg acacccggtg
     7621 tgggtttaga tgacaaggga gacgcattgg gtcaacagta tagaaccgtg gatgatgtgg
     7681 tctctacagg atctgacatt attattgttg gaagaggact atttgcaaag ggaagggatg
     7741 ctaaggtaga gggtgaacgt tacagaaaag caggctggga agcatatttg agaagatgcg
     7801 gccagcaaaa ctaaaaaact gtattataag taaatgcatg tatactaaac tcacaaatta
     7861 gagcttcaat ttaattatat cagttattac ccattgaaaa aggaagagta tgagtattca
     7921 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
     7981 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta
     8041 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt
     8101 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc
     8161 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc
     8221 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc
     8281 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa
     8341 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga
     8401 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat
     8461 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca
     8521 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
     8581 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat
     8641 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
     8701 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
     8761 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
     8821 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
     8881 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     8941 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     9001 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     9061 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
     9121 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     9181 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     9241 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     9301 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     9361 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     9421 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     9481 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     9541 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
     9601 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
     9661 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
     9721 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagggccg cagcttgcaa
     9781 attaaagcct tcgagcgtcc caaaaccttc tcaagcaagg ttttcagtat aatgttacat
     9841 gcgtacacgc gtctgtacag aaaaaaaaga aaaatttgaa atataaataa cgttcttaat
     9901 actaacataa ctataaaaaa ataaataggg acctagactt caggttgtct aactccttcc
     9961 ttttcggtta gagcggatgt ggggggaggg cgtgaatgta agcgtgacat aactaattac
    10021 atgatgcggc cctctagatg catgctcgag cggccgcctt agtggtggtg gtggtggtgc
    10081 gcgctgctgc cgcggggaac taaaccgctg ctctgatcgt ttgggtccaa ggcatcgtaa
    10141 gtcgggcata agtcactcca ggcctgaggg actgggttca cagcctttac gtcgggcagg
    10201 tcctcggcca ttactacagc gaatccagct tccaagtggt agtcgatgtg acagtgcaga
    10261 aaccacggtc cggggttgtt agtgtcaaat ctaatggtaa cgttgtctcc cgcagctggc
    10321 gttccggtgt cgactacgtc acggaatata gggttatcat aattataaac agttgaaccg
    10381 gccgacctca caactgcgaa agtgtgtccg tgtaagtgga atgggtgtgg ggctccaggg
    10441 gccgccgcgg tggcagggaa ggaaatctcg atggtagcat tcgaaggcaa tacatagacg
    10501 ctgcctgatg gcaacaagtc ttgtgcagtc tgcgctcccg atagaatctg cagcagcacg
    10561 ggtaccgtgg gcgggacaaa cgagacgcca ttaataaaga agttagagcc attgaagtta
    10621 aatgccatgt taattgcctt gtccacacca cccggcgatg gactgccggg gacgggcgta
    10681 ggcactagag gatgtaagtc ggtctcgttt aagggtttca ctgaagttgt ttgattagtt
    10741 gtgggttcca ccgcgggcgc tccatcgtaa cgcaggatag cagaattaat tccgccgtcg
    10801 aagccgacat taccgaaatt tgggttagcc ctgatccagt agttgtcaac gggctgatta
    10861 gcgtccaaca cgaaagagta cctctgtgcg gcaaatattt ggatagagtc aacctcaagt
    10921 ggttgaacgt ttacggaatc tacctctatg atggtcaggt tgtgtccgtc tattgaaaat
    10981 gtgaagtttg gctcgcatga taatgacact aaacggaaac ggtacctctt accctttgtt
    11041 accttaataa cagatagctc tgcggtaggg tcgctcggtg ctctaccctt tccattgatt
    11101 aaagttgcgt cagctcctgg cggaaacctg ggtcccaact ttgcagccgt gtggtaccag
    11161 tccgccagtg ttattactgt gctgtcatta tctacatcat acatactagc gtgtgggtcg
    11221 tttgggtcat acacaacaaa agggcccctt aatccatcgc agtactgcgt gcttagatga
    11281 gaatgatacc aaaaggtacc cgcttggtca ggtacttgga aatcatatag aaatgaatgc
    11341 cccggtgcta ttgggcattg gtttatgaaa gctgggccgt cggcccaatt cgttccgtgc
    11401 tggaagaatc cgtgccagtg aatagaagta gattttagca tggtgtgatt tgtaaggttg
    11461 tttattacat ttaactggaa tctgtcgccc ttgttgccgg cgattagtgg tccgggcgtc
    11521 acgccgttga ccacgactgc ttgccttgag aaaccgtcag ggctcactac ggcatctgtg
    11581 atggttagat ctgcgacggg gcctaccgcg aactcagctt cagtctctct tttctcgaga
    11641 gatacccctt cttctttagc agcaatgctg gcaatagtag tatttataaa caataatccg
    11701 ttatttgtgc tgttggaaaa tggcaaaaca gcaacatcga aatccccttc taaatctgag
    11761 taaccgatga cagcttcagc cggaatttgt gccgtttcat cttctgttgt agttttgact
    11821 ggagcagcta atgcggagga tgctgcgaat aaatcagcag taaaaattga aggaaatctc
    11881 atggatccta tggatcctat agttttttct ccttgacgtt aaagtataga ggtatattaa
    11941 caattttttg ttgatacttt tattacattt gaataagaag taatacaaac cgaaaatgtt
    12001 gaaagtatta gttaaagtgg ttatgcagtt tttgcattta tatatctgtt aatagatcaa
    12061 aaatcatcgc ttcgctgatt aattacccca gaaataaggc taaaaaacta atcgcattat
    12121 catcctatgg ttgttaattt gattcgttca tttgaaggtt tgtggggcca ggttactgcc
    12181 aatttttcct cttcataacc ataaaagcta gtattgtaga atctttattg ttcggagcag
    12241 tgcggcgcga ggcacatctg cgtttcagga acgcgaccgg tgaagacgag gacgcacgga
    12301 ggagagtctt ccttcggagg gctgtcaccc gctcggcggc ttctaatccg tggactagtg
    12361 gatcgatccc c
//
