LOCUS       sequence-370141-       17544 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  FuncLib-designed high-redox potential laccase from Trametes hirsuta 
            (Th3.14).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 17544)
  AUTHORS   Barber-Zucker S, Mateljak I, Goldsmith M, Kupervaser M, Alcalde M, 
            Fleishman SJ
  TITLE     Designed High-Redox Potential Laccases Exhibit High Functional 
            Diversity.
  JOURNAL   ACS Catal. 2022 Nov 4;12(21):13164-13173. doi: 
            10.1021/acscatal.2c03006. Epub 2022 Oct 13.
  PUBMED    36366766
REFERENCE   2  (bases 1 to 17544)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acscatal.2c03006"; journalName: "ACS Catal"; date: 
            "2022-11-4- 4"; volume: "12"; issue: "21"; pages: "13164-13173"
COMMENT     Sequence Label: pJRoC30_Th3.14
FEATURES             Location/Qualifiers
     source          1..17544
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     547..564
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(641..888)
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     854..872
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     CDS             complement(925..942)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(949..966)
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             complement(join(2479..2490,2491..2688,2689..2745))
                     /codon_start=1
                     /gene="MF-alpha-1"
                     /product="N-terminal secretion signal from S. cerevisiae 
                     alpha-factor"
                     /label=alpha-factor secretion signal
                     /note="Cleavage by the Kex2 protease occurs after the 
                     dibasic KR sequence. The EA dipeptides are then removed by 
                     dipeptidyl aminopeptidase A."
                     /translation="MRFPSIFTADLFAASSALAAPVKTTTEDETAQIPAEAVIGYSDLE
                     GDFDVAVLPFSNSTNNGLLFINTTIASIAAKEEGVSLEKRETEA"
     promoter        complement(2772..3213)
                     /gene="S. cerevisiae GAL1"
                     /label=GAL1 promoter
                     /note="inducible promoter, regulated by Gal4"
     primer_bind     complement(2778..2801)
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     protein_bind    3096..3213
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     rep_origin      3235..3690
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3322..3341)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3532..3553
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     protein_bind    complement(4018..4065)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             5658..6929
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=FLP
                     /note="FLP is a site-specific recombinase from 
                     Saccharomyces cerevisiae. Recombination occurs at FRT 
                     sequences."
                     /translation="MPQFDILCKTPPKVLVRQFVERFERPSGEKIALCAAELTYLCWMI
                     THNGTAIKRATFMSYNTIISNSLSFDIVNKSLQFKYKTQKATILEASLKKLIPAWEFTI
                     IPYYGQKHQSDITDIVSSLQLQFESSEEADKGNSHSKKMLKALLSEGESIWEITEKILN
                     SFEYTSRFTKTKTLYQFLFLATFINCGRFSDIKNVDPKSFKLVQNKYLGVIIQCLVTET
                     KTSVSRHIYFFSARGRIDPLVYLDEFLRNSEPVLKRVNRTGNSSSNKQEYQLLKDNLVR
                     SYNKALKKNAPYSIFAIKNGPKSHIGRHLMTSFLSMKGLTELTNVVGNWSDKRASAVAR
                     TTYTHQITAIPDHYFALVSRYYAYDPISKEMIALKDETNPIEEWQHIEQLKGSAEGSIR
                     YPAWNGIISQEVLDYLSSYINRRI"
     protein_bind    7082..7129
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     promoter        10029..10244
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     CDS             10245..11048
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     CDS             join(11144..11212,11213..12004)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(11362..11381)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      12175..12763
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12916..12938
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     12935..12951
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12935..12951
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    13008..13107
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             15451..15462
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     regulatory      15907..15916
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(15917..16016)
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(16069..16086)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(16069..16085)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(16078..16100)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     terminator      complement(16214..16260)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     complement(16261..16277)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(16261..16277)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(16387..16406)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             16398..17213
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             16398..17213
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(16471..16490)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      join(17349..17544,1..393)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
       61 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
      121 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
      181 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
      241 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
      301 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
      361 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
      421 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
      481 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
      541 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc
      601 cccgcgcgtt ggccgattca ttaatgcagg gccgcagctt gcaaattaaa gccttcgagc
      661 gtcccaaaac cttctcaagc aaggttttca gtataatgtt acatgcgtac acgcgtctgt
      721 acagaaaaaa aagaaaaatt tgaaatataa ataacgttct taatactaac ataactataa
      781 aaaaataaat agggacctag acttcaggtt gtctaactcc ttccttttcg gttagagcgg
      841 atgtgggggg agggcgtgaa tgtaagcgtg acataactaa ttacatgatg cggccctcta
      901 gatgcatgct cgagcggccg cttagtggtg gtggtggtgg tgcgcgctgc tgccgcgggg
      961 aactaaaccg ctgctctgat cgtttgggtc caaggcatcg taagtcgggc ataagtcact
     1021 ccaggcctga gggactgggt tcacagcctt tacgtcgggc aggtcctcgg ccattactac
     1081 ggagaagccg gcctcaaggt gccagtctat gtggcagtgt aggaaccagg gccccgggtt
     1141 attcgtgtcg aatcttatcg tgacattatc gccggctgcg ggggtgcctg tattaactac
     1201 atctctgaaa atcggattgt cgtaattata aacggttgat ccggctgacc taaccacggc
     1261 aaatgtgtgc ccgtgcagat gaaaggggtg tggggcacca ggagcagctg cagtggcggg
     1321 gaatgagatc tctatggtag cattactagg aagaacgtag actgaaccgg acggtaacaa
     1381 gtcctgggcg gtctgtgccc cactcaagat ctgtagtaag acagggactg taggcggaac
     1441 gaaagaaacc ccattgatga agaagttact gccgttgaag ttgaacgcca tgtttatagc
     1501 tttatcaact ccacctgggc ttggagaacc cgggacaggc gtgggtacta atggatgcag
     1561 gtcagtctca ttaagtggct ttactgacgt agtttgattt gttgtcggtt ccacggctgg
     1621 agcgccgtca tatctcagta tggcgctgtt gattccaccg tcaaagccaa cgtttccgaa
     1681 gttagggttc gctctgatcc aatagttgtc aacgggctgg tttgcatcaa ggacgaaaga
     1741 atatctttga gctgcgaaaa tctgtatcga gtcaacctct aatggctgta cgttaaccga
     1801 gtccacctct atgattgtca aattatgccc gtctattgag aaggtgaagt ttgggtggca
     1861 ggatagtgac accagcctga acctgtatct cttccccttg gtaaccttta tgacagacaa
     1921 ctcggctgtg ggatcactgg gtgctcttcc tttaccgtta attaaagtgg catcagcacc
     1981 aggtggaaac ctgggtccca actttgcagc cgtgtggtac cagtccgcca gtgttattac
     2041 tgtgctgtca ttatctacat catacatact agcgtgtggg tcgtttgggt catacacaac
     2101 aaaagggccc cttaatccat cgcagtactg cgtgcttaga tgagaatgat accaaaaggt
     2161 acccgcttgg tcaggtactt ggaaatcata tagaaatgaa tgccccggtg ctattgggca
     2221 ttggtttatg aaagctgggc cgtcggccca attcgttccg tgctggaaga atccgtgcca
     2281 gtgaatagaa gtagatttta gcatggtgtg atttgtaagg ttgtttatta catttaactg
     2341 gaatctgtcg cccttgttgc cggcgattag tggtccgggc gtcacgccgt tgaccacgac
     2401 tgcttgcctt gagaaaccgt cagggctcac tacggcatct gtgatggtta gatctgcgac
     2461 ggggcctacc gcgaactcag cttcagtctc tcttttctcg agagataccc cttcttcttt
     2521 agcagcaatg ctggcaatag tagtatttat aaacaataat ccgttatttg tgctgttgga
     2581 aaatggcaaa acagcaacat cgaaatcccc ttctaaatct gagtaaccga tgacagcttc
     2641 agccggaatt tgtgccgttt catcttctgt tgtagttttg actggagcag ctaatgcgga
     2701 ggatgctgcg aataaatcag cagtaaaaat tgaaggaaat ctcatggatc ctatggatcc
     2761 tatagttttt tctccttgac gttaaagtat agaggtatat taacaatttt ttgttgatac
     2821 ttttattaca tttgaataag aagtaataca aaccgaaaat gttgaaagta ttagttaaag
     2881 tggttatgca gtttttgcat ttatatatct gttaatagat caaaaatcat cgcttcgctg
     2941 attaattacc ccagaaataa ggctaaaaaa ctaatcgcat tatcatccta tggttgttaa
     3001 tttgattcgt tcatttgaag gtttgtgggg ccaggttact gccaattttt cctcttcata
     3061 accataaaag ctagtattgt agaatcttta ttgttcggag cagtgcggcg cgaggcacat
     3121 ctgcgtttca ggaacgcgac cggtgaagac gaggacgcac ggaggagagt cttccttcgg
     3181 agggctgtca cccgctcggc ggcttctaat ccgtggacta gtggatcgat ccccacgcgc
     3241 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac
     3301 ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg
     3361 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt
     3421 tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc
     3481 cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct
     3541 tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga
     3601 ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga
     3661 attttaacaa aatattaacg cttacaattt ctctacgtat aggaaatgtt tacattttcg
     3721 tattgttttc gattcactct atgaatagtt cttactacaa tttttttgtc taaagagtaa
     3781 tactagagat aaacataaaa aatgtagagg tcgagtttag atgcaagttc aaggagcgaa
     3841 aggtggatgg gtaggttata tagggatata gcacagagat atatagcaaa gagatacttt
     3901 tgagcaatgt ttgtggaagc ggtattcgca atattttagt agctcgttac agtccggtgc
     3961 gtttttggtt ttttgaaagt gcgtcttcag agcgcttttg gttttcaaaa gcgctctgaa
     4021 gttcctatac tttctagaga ataggaactt cggaatagga acttcaaagc gtttccgaaa
     4081 acgagcgctt ccgaaaatgc aacgcgagct gcgcacatac agctcactgt tcacgtcgca
     4141 cctatatctg cgtgttgcct gtatatatat atacatgaga agaacggcat agtgcgtgtt
     4201 tatgcttaaa tgcgtactta tatgcgtcta tttatgtagg atgaaaggta gtctagtacc
     4261 tcctgtgata ttatcccatt ccatgcgggg tatcgtatgc ttccttcagc actacccttt
     4321 agctgttcta tatgctgcca ctcctcaatt ggattagtct catccttcaa tgctatcatt
     4381 tcctttgata ttggatcata ccctagaagt attacgtgat tttctgcccc ttaccctcgt
     4441 tgctactctc ctttttttcg tgggaaccgc tttagggccc tcagtgatgg tgttttgtaa
     4501 tttatatgct cctcttgcat ttgtgtctct acttcttgtt cgcctggagg gaacttcttc
     4561 atttgtatta gcatggttca cttcagtcct tccttccaac tcactctttt tttgctgtaa
     4621 acgattctct gccgccagtt cattgaaact attgaatata tcctttagag attccgggat
     4681 gaataaatca cctattaaag cagcttgacg atctggtgga actaaagtaa gcaattgggt
     4741 aacgacgctt acgagcttca taacatcttc ttccgttgga gctggtggga ctaataactg
     4801 tgtacaatcc atttttctca tgagcatttc ggtagctctc ttcttgtctt tctcgggcaa
     4861 tcttcctatt attatagcaa tagatttgta tagttgcttt ctattgtcta acagcttgtt
     4921 attctgtagc atcaaatcta tggcagcctg acttgcttct tgtgaagaga gcataccatt
     4981 tccaatcgaa tcaaaccttt ccttaaccat cttcgcagca ggcaaaatta cctcagcact
     5041 ggagtcagaa gatacgctgg aatcttctgc gctagaatca agaccatacg gcctaccggt
     5101 tgtgagagat tccatgggcc ttatgacata tcctggaaag agtagctcat cagacttacg
     5161 tttactctct atatcaatat ctacatcagg agcaatcatt tcaataaaca gccgacatac
     5221 atcccagacg ctataagctg tacgtgcttt taccgtcaga ttcttggctg tttcaatgtc
     5281 gtccattttg gttttctttt accagtattg ttcgtttgat aatgtattct tgcttattac
     5341 attataaaat ctgtgcagat cacatgtcaa aacaactttt tatcacaaga tagtaccgca
     5401 aaacgaacct gcgggccgtc taaaaattaa ggaaaagcag caaaggtgca tttttaaaat
     5461 atgaaatgaa gataccgcag taccaattat tttcgcagta caaataatgc gcggccggtg
     5521 catttttcga aagaacgcga gacaaacagg acaattaaag ttagtttttc gagttagcgt
     5581 gtttgaatac tgcaagatac aagataaata gagtagttga aactagatat caattgcaca
     5641 caagatcggc gctaagcatg ccacaatttg atatattatg taaaacacca cctaaggtgc
     5701 ttgttcgtca gtttgtggaa aggtttgaaa gaccttcagg tgagaaaata gcattatgtg
     5761 ctgctgaact aacctattta tgttggatga ttacacataa cggaacagca atcaagagag
     5821 ccacattcat gagctataat actatcataa gcaattcgct gagtttcgat attgtcaata
     5881 aatcactcca gtttaaatac aagacgcaaa aagcaacaat tctggaagcc tcattaaaga
     5941 aattgattcc tgcttgggaa tttacaatta ttccttacta tggacaaaaa catcaatctg
     6001 atatcactga tattgtaagt agtttgcaat tacagttcga atcatcggaa gaagcagata
     6061 agggaaatag ccacagtaaa aaaatgctta aagcacttct aagtgagggt gaaagcatct
     6121 gggagatcac tgagaaaata ctaaattcgt ttgagtatac ttcgagattt acaaaaacaa
     6181 aaactttata ccaattcctc ttcctagcta ctttcatcaa ttgtggaaga ttcagcgata
     6241 ttaagaacgt tgatccgaaa tcatttaaat tagtccaaaa taagtatctg ggagtaataa
     6301 tccagtgttt agtgacagag acaaagacaa gcgttagtag gcacatatac ttctttagcg
     6361 caaggggtag gatcgatcca cttgtatatt tggatgaatt tttgaggaat tctgaaccag
     6421 tcctaaaacg agtaaatagg accggcaatt cttcaagcaa taaacaggaa taccaattat
     6481 taaaagataa cttagtcaga tcgtacaata aagctttgaa gaaaaatgcg ccttattcaa
     6541 tctttgctat aaaaaatggc ccaaaatctc acattggaag acatttgatg acctcatttc
     6601 tttcaatgaa gggcctaacg gagttgacta atgttgtggg aaattggagc gataagcgtg
     6661 cttctgccgt ggccaggaca acgtatactc atcagataac agcaatacct gatcactact
     6721 tcgcactagt ttctcggtac tatgcatatg atccaatatc aaaggaaatg atagcattga
     6781 aggatgagac taatccaatt gaggagtggc agcatataga acagctaaag ggtagtgctg
     6841 aaggaagcat acgatacccc gcatggaatg ggataatatc acaggaggta ctagactacc
     6901 tttcatccta cataaataga cgcatataag tacgcattta agcataaaca cgcactatgc
     6961 cgttcttctc atgtatatat atatacaggc aacacgcaga tataggtgcg acgtgaacag
     7021 tgagctgtat gtgcgcagct cgcgttgcat tttcggaagc gctcgttttc ggaaacgctt
     7081 tgaagttcct attccgaagt tcctattctc tagaaagtat aggaacttca gagcgctttt
     7141 gaaaaccaaa agcgctctga agacgcactt tcaaaaaacc aaaaacgcac cggactgtaa
     7201 cgagctacta aaatattgcg aataccgctt ccacaaacat tgctcaaaag tatctctttg
     7261 ctatatatct ctgtgctata tccctatata acctacccat ccacctttcg ctccttgaac
     7321 ttgcatctaa actcgacctc tacatcaaca ggcttccaat gctcttcaaa ttttactgtc
     7381 aagtagaccc atacggctgt aatatgctgc tcttcataat gtaagcttat ctttatcgaa
     7441 tcgtgtgaaa aactactacc gcgataaacc tttacggttc cctgagattg aattagttcc
     7501 tttagtatat gatacaagac acttttgaac tttgtacgac gaattttgag gttcgccatc
     7561 ctctggctat ttccaattat cctgtcggct attatctccg cctcagtttg atcttccgct
     7621 tcagactgcc atttttcaca taatgaatct atttcacccc acaatccttc atccgcctcc
     7681 gcatcttgtt ccgttaaact attgacttca tgttgtacat tgtttagttc acgagaaggg
     7741 tcctcttcag gcggtagctc ctgatctcct atatgacctt tatcctgttc tctttccaca
     7801 aacttagaaa tgtattcatg aattatggag cacctaataa cattcttcaa ggcggagaag
     7861 tttgggccag atgcccaata tgcttgacat gaaaacgtga gaatgaattt agtattattg
     7921 tgatattctg aggcaatttt attataatct cgaagataag agaagaatgc agtgaccttt
     7981 gtattgacaa atggagattc catgtatcta aaaaatacgc ctttaggcct tctgataccc
     8041 tttcccctgc ggtttagcgt gccttttaca ttaatatcta aaccctctcc gatggtggcc
     8101 tttaactgac taataaatgc aaccgatata aactgtgata attctgggtg atttatgatt
     8161 cgatcgacaa ttgtattgta cactagtgca ggatcaggcc aatccagttc tttttcaatt
     8221 accggtgtgt cgtctgtatt cagtacatgt ccaacaaatg caaatgctaa cgttttgtat
     8281 ttcttataat tgtcaggaac tggaaaagtc ccccttgtcg tctcgattac acacctactt
     8341 tcatcgtaca ccataggttg gaagtgctgc ataatacatt gcttaataca agcaagcagt
     8401 ctctcgccat tcatatttca gttattttcc attacagctg atgtcattgt atatcagcgc
     8461 tgtaaaaatc tatctgttac agaaggtttt cgcggttttt ataaacaaaa ctttcgttac
     8521 gaaatcgagc aatcacccca gctgcgtatt tggaaattcg ggaaaaagta gagcaacgcg
     8581 agttgcattt tttacaccat aatgcatgat taacttcgag aagggattaa ggctaatttc
     8641 actagtatgt ttcaaaaacc tcaatctgtc cattgaatgc cttataaaac agctatagat
     8701 tgcatagaag agttagctac tcaatgcttt ttgtcaaagc ttactgatga tgatgtgtct
     8761 actttcaggc gggtctgtag taaggagaat gacattataa agctggcact tagaattcca
     8821 cggactatag actatactag tatactccgt ctactgtacg atacacttcc gctcaggtcc
     8881 ttgtccttta acgaggcctt accactcttt tgttactcta ttgatccagc tcagcaaagg
     8941 cagtgtgatc taagattcta tcttcgcgat gtagtaaaac tagctagacc gagaaagaga
     9001 ctagaaatgc aaaaggcact tctacaatgg ctgccatcat tattatccga tgtgacgctg
     9061 cagcttctca atgatattcg aatacgcttt gaggagatac agcctaatat ccgacaaact
     9121 gttttacaga tttacgatcg tacttgttac ccatcattga attttgaaca tccgaacctg
     9181 ggagttttcc ctgaaacaga tagtatattt gaacctgtat aataatatat agtctagcgc
     9241 tttacggaag acaatgtatg tatttcggtt cctggagaaa ctattgcatc tattgcatag
     9301 gtaatcttgc acgtcgcatc cccggttcat tttctgcgtt tccatcttgc acttcaatag
     9361 catatctttg ttaacgaagc atctgtgctt cattttgtag aacaaaaatg caacgcgaga
     9421 gcgctaattt ttcaaacaaa gaatctgagc tgcattttta cagaacagaa atgcaacgcg
     9481 aaagcgctat tttaccaacg aagaatctgt gcttcatttt tgtaaaacaa aaatgcaacg
     9541 cgagagcgct aatttttcaa acaaagaatc tgagctgcat ttttacagaa cagaaatgca
     9601 acgcgagagc gctattttac caacaaagaa tctatacttc ttttttgttc tacaaaaatg
     9661 catcccgaga gcgctatttt tctaacaaag catcttagat tacttttttt ctcctttgtg
     9721 cgctctataa tgcagtctct tgataacttt ttgcactgta ggtccgttaa ggttagaaga
     9781 aggctacttt ggtgtctatt ttctcttcca taaaaaaagc ctgactccac ttcccgcgtt
     9841 tactgattac tagcgaagct gcgggtgcat tttttcaaga taaaggcatc cccgattata
     9901 ttctataccg atgtggattg cgcatacttt gtgaacagaa agtgatagcg ttgatgattc
     9961 ttcattggtc agaaaattat gaacggtttc ttctattttg tctctatata ctacgtacct
    10021 gctagctttt caattcatca tttttttttt attctttttt ttgatttcgg tttccttgaa
    10081 atttttttga ttcggtaatc tccgaacaga aggaagaacg aaggaaggag cacagactta
    10141 gattggtata tatacgcata tgtagtgttg aagaaacatg aaattgccca gtattcttaa
    10201 cccaactgca cagaacaaaa acctgcagga aacgaagata aatcatgtcg aaagctacat
    10261 ataaggaacg tgctgctact catcctagtc ctgttgctgc caagctattt aatatcatgc
    10321 acgaaaagca aacaaacttg tgtgcttcat tggatgttcg taccaccaag gaattactgg
    10381 agttagttga agcattaggt cccaaaattt gtttactaaa aacacatgtg gatatcttga
    10441 ctgatttttc catggagggc acagttaagc cgctaaaggc attatccgcc aagtacaatt
    10501 ttttactctt cgaagacaga aaatttgctg acattggtaa tacagtcaaa ttgcagtact
    10561 ctgcgggtgt atacagaata gcagaatggg cagacattac gaatgcacac ggtgtggtgg
    10621 gcccaggtat tgttagcggt ttgaagcagg cggcagaaga agtaacaaag gaacctagag
    10681 gccttttgat gttagcagaa ttgtcatgca agggctccct atctactgga gaatatacta
    10741 agggtactgt tgacattgcg aagagcgaca aagattttgt tatcggcttt attgctcaaa
    10801 gagacatggg tggaagagat gaaggttacg attggttgat tatgacaccc ggtgtgggtt
    10861 tagatgacaa gggagacgca ttgggtcaac agtatagaac cgtggatgat gtggtctcta
    10921 caggatctga cattattatt gttggaagag gactatttgc aaagggaagg gatgctaagg
    10981 tagagggtga acgttacaga aaagcaggct gggaagcata tttgagaaga tgcggccagc
    11041 aaaactaaaa aactgtatta taagtaaatg catgtatact aaactcacaa attagagctt
    11101 caatttaatt atatcagtta ttacccattg aaaaaggaag agtatgagta ttcaacattt
    11161 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
    11221 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
    11281 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat
    11341 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca
    11401 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
    11461 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
    11521 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct
    11581 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
    11641 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac
    11701 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
    11761 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
    11821 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc
    11881 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
    11941 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
    12001 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
    12061 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
    12121 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
    12181 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
    12241 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
    12301 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa
    12361 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
    12421 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
    12481 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
    12541 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
    12601 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
    12661 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
    12721 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
    12781 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc cwgcgwyatm
    12841 ysccatacgc cgaactgaac tccacctaat gtcttggaga ttgtcggata gcgcggaata
    12901 ggattgtgcc ctctgagcgg ataacaattt cacacaggaa acagctatga ccatgacaca
    12961 tgcatccacc atcgcagact tatcatcact gagcctccac ctagcctcaa ataatgattt
    13021 tattttgact gatagtgacc tgttcgttgc aacaaattga taagcaatgc tttcttataa
    13081 tgccaacttt gtacaagaaa gctgggtcac cggttaagtc gctagcagtc tggtttcttg
    13141 ccggcgggcc agagaactcc ttccgaataa tttcgtttac gtcatccaga tatgtctggt
    13201 cgaggttctg ttcttgtttg attgtcactc ccgcggggag cacctccttt tgatctgaca
    13261 ccggaggtcc gttcttagcg gctctttggc tggtggcgtt ctgttgctgg ataactgttg
    13321 ggtagctacc tggactcagc cgctggcctt ggcttactgg cggcgggcca ggccgtgttg
    13381 ttcctggtgc gaagttttcg caatacataa tgtgttgaaa ctcttgatga gacccacacc
    13441 gcaactgttg gttggtaggg ctatagtgta ttacgggaga agcctgctga tttgtaggac
    13501 ttgggtgcaa aagggcgctg gattgtcctt gtgagcccgc atgaaccagc acgctcctat
    13561 gtgcatccgc caaagacagc ggggccgcca tgagggccgg ttgctggtac cccaacaagg
    13621 acggtgagag actcttggac ctctggtaaa gaacggcagc ggggttttgt tgctgatagc
    13681 gtgcgtcagg acttgacagt ccggttctga attgttggca cggtgccata gtagctacca
    13741 gacaagaggg ggactcggcg accatcgggt gttgtgggta atagggctga ctgccgagtc
    13801 ctccgtgcgt tgggctacag ataagtgtgg gatcgtattc atcagttggt tctgttttga
    13861 ttgctgggac cggatggtat gtgaaatgtt gaggttgaga tcgtttccgc ttcccattga
    13921 tgacataaaa gttcaccttt actggtgttc gaatgtgttt attcctgtat tcggggattt
    13981 ctacaaaaag catgttgggc tgtgacttat ctttatctac agtggcttcc atctcccata
    14041 tttgctgacc atcggtcgtt ttttcagtga aaacaacttt agattcggac gtaaagtttt
    14101 ggccagtaag gatcatttgc tgtccaccgt aaaccagaca tgagtcagta tcttgtcttt
    14161 ctaccatagg gagctcatga gcgcttcgtt ggctacactc aattgggtta ctcgctgtct
    14221 ggagtgagac gatgcgacca gaactttccg ggatatgaac ccgaaatacc aagcgcacgc
    14281 gcgtattttt tctgccaatg tctgtttcac cctttctcaa ttcgatatcg gcatttcgga
    14341 gcttcagtat acccgcgcag tcaatcgtag ctcgcatatt gttttttggc tcgaggggaa
    14401 tctccagcac cttagtgttg ccaactattt tctcataact cgtagtcgtg acggttttgc
    14461 cggtgatcct atgcacctgg taaaaggcgt ggggcttcaa tatccgctca tcggccgttc
    14521 cgatgaatat ctggaggccg agcggtttgt tttccatata cccatgcaac tgcacgactg
    14581 ggtggccccc agtcgaagct ttgaccgctc ctcggctggc tgcagtttca gcgtgcgccg
    14641 catgatgcgg ttttggttgt acttcgatcc tcaattcgta ggtaccactt tgtgatgaga
    14701 gcggccactc caagggaggc agtgaggccg ttactgggat tgaacagatc gggatagctg
    14761 gaacaagtgg cttgggccat gttgggggta cgagaaggat actctctggc gcactatttc
    14821 tgcgttctcc ttgttcacac ggcccaagaa attctactgc cgggtagata tgccttggaa
    14881 gtcccgcctt agatggagca gcactgaccg gggatggatc cggtgacgtt ttccacattt
    14941 tcggcggaat cccgcaggga gaatccgttg ccaaactatt aagggcgtcc atgataacgg
    15001 cagaccctgc cactggggga tagcctgcgg gacttccgtg gtcttggagg gccacatgag
    15061 ctgcgggttg cggcgccaat gatcgggccc tctgcggggc tgcgcctgga ggcaatgcta
    15121 cgagggcttc agcacaagaa tgccttcgct ttgccccggg tgccgctgcc cggcttgcgg
    15181 gtctaggcac aggagagtgt cgacccaaac aagaatcttc cgcaagggaa gttctgggtg
    15241 ccattatggg ggccgtgcga ggggcataat gagcggggat gttttggaac tgagggcaca
    15301 agtcatcggg gcctccgttg ttaggtgaaa cgcatgggct ggtgtacgga gagaacgtgt
    15361 cactgataaa tgctgccgcg gcaccggctg ccgccggtga cagacagagt ggttcgcgat
    15421 acccctcgaa tccaggcacc gggagtgtaa atcgagggga ggctgctaca cctgcaagcg
    15481 gcggttgttc tacaagcaaa ccggcatcgc gcatccgcaa gggtccaacg gcctgaatca
    15541 attcgtgact tggagttatc tcaattctag gactgagtcc agatgcaccc gcgggtttcg
    15601 ctgcactcaa gaatttttgc ggccctactc ggtccggctc gccgaagcga cctgggggct
    15661 ctccggaaag gctcgcgagg ggtgaatagg gtttcaggcc gtaatcgagt acatcgtccg
    15721 ggtatgccgg gccagacggt ggagaggcga ctttgtgagc attgggctcc tcctcattcg
    15781 ggttcaggta ttcataatca aacaatatag agaaatcaag ttcgtcttgc ggactcccac
    15841 caggttcatg acctggagca tcaccgccat ctggttgtgg ctgcctttcg ggcgcgtttc
    15901 tagacaccat ggtggcagcc tgcttttttg tacaaagttg gcattataaa aaagcattgc
    15961 tcatcaatct gttgcaacga acaggtcact atcagtcaaa ataaaatcat tatttgaggt
    16021 caggcggaat ggcacttcct tattccagat gcgtgcggat tatgcatgac tggccgtcgt
    16081 tttacaacgt cgtgactggg aaaaccctgg cgcttgtggt ggatcacagt tattggagac
    16141 ttccttggcc gtacgatggg ttttgtttca ggtatcccaa cctggacgtc tcgtataatg
    16201 cgacaaacaa cagataaaac gaaaggccca gtctttcgac tgagcctttc gttttatttg
    16261 gatccactag ttctagagcg gcgggggaaa gccacgttgt gtctcaaaat ctctgatgtt
    16321 acattgcaca agataaaaat atatcatcat gaacaataaa actgtctgct tacataaaca
    16381 gtaatacaag gggtgttatg agccatattc aacgggaaac gtcttgctcg aggccgcgat
    16441 taaattccaa catggatgct gatttatatg ggtataaatg ggctcgcgat aatgtcgggc
    16501 aatcaggtgc gacaatctat cgattgtatg ggaagcccga tgcgccagag ttgtttctga
    16561 aacatggcaa aggtagcgtt gccaatgatg ttacagatga gatggtcaga ctaaactggc
    16621 tgacggaatt tatgcctctt ccgaccatca agcattttat ccgtactcct gatgatgcat
    16681 ggttactcac cactgcgatc cccgggaaaa cagcattcca ggtattagaa gaatatcctg
    16741 attcaggtga aaatattgtt gatgcgctgg cagtgttcct gcgccggttg cattcgattc
    16801 ctgtttgtaa ttgtcctttt aacagcgatc gcgtatttcg tctcgctcag gcgcaatcac
    16861 gaatgaataa cggtttggtt gatgcgagtg attttgatga cgagcgtaat ggctggcctg
    16921 ttgaacaagt ctggaaagaa atgcataagc ttttgccatt ctcaccggat tcagtcgtca
    16981 ctcatggtga tttctcactt gataacctta tttttgacga ggggaaatta ataggttgta
    17041 ttgatgttgg acgagtcgga atcgcagacc gataccagga tcttgccatc ctatggaact
    17101 gcctcggtga gttttctcct tcattacaga aacggctttt tcaaaaatat ggtattgata
    17161 atcctgatat gaataaattg cagtttcatt tgatgctcga tgagtttttc taatcagaat
    17221 ttgttaattg gttgtaacac tggcagagcg gacgatttga agccccctac ccatttacgc
    17281 tgacttgacg ggacggcggc tttgttgaat agagcgtcag accccgtaga aaagatcaaa
    17341 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
    17401 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
    17461 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc
    17521 caccacttca agaactctgt agca
//
