LOCUS       sequence-370143-       12293 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  FuncLib-designed high-redox potential laccase from Trametes hirsuta 
            (Th3.7).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12293)
  AUTHORS   Barber-Zucker S, Mateljak I, Goldsmith M, Kupervaser M, Alcalde M, 
            Fleishman SJ
  TITLE     Designed High-Redox Potential Laccases Exhibit High Functional 
            Diversity.
  JOURNAL   ACS Catal. 2022 Nov 4;12(21):13164-13173. doi: 
            10.1021/acscatal.2c03006. Epub 2022 Oct 13.
  PUBMED    36366766
REFERENCE   2  (bases 1 to 12293)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acscatal.2c03006"; journalName: "ACS Catal"; date: 
            "2022-11-4- 4"; volume: "12"; issue: "21"; pages: "13164-13173"
COMMENT     Sequence Label: pJRoC30_Th3.7
FEATURES             Location/Qualifiers
     source          1..12293
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    778..825
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     rep_origin      complement(1153..1608)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1290..1311)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     1502..1521
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        1630..2071
                     /gene="S. cerevisiae GAL1"
                     /label=GAL1 promoter
                     /note="inducible promoter, regulated by Gal4"
     protein_bind    1630..1747
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     primer_bind     2042..2065
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     CDS             join(2097..2153,2154..2351,2352..2363)
                     /codon_start=1
                     /gene="MF-alpha-1"
                     /product="N-terminal secretion signal from S. cerevisiae 
                     alpha-factor"
                     /label=alpha-factor secretion signal
                     /note="Cleavage by the Kex2 protease occurs after the 
                     dibasic KR sequence. The EA dipeptides are then removed by 
                     dipeptidyl aminopeptidase A."
                     /translation="MRFPSIFTADLFAASSALAAPVKTTTEDETAQIPAEAVIGYSDLE
                     GDFDVAVLPFSNSTNNGLLFINTTIASIAAKEEGVSLEKRETEA"
     CDS             3876..3893
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             3900..3917
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      3954..4201
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(3970..3988)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     primer_bind     complement(4278..4295)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4449..5037)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4529..4548)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5208..5999,6000..6068))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5831..5850
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     CDS             complement(6164..6967)
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     promoter        complement(6968..7183)
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     protein_bind    complement(10083..10130)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
ORIGIN
        1 aagagagcta ccgaaatgct catgagaaaa atggattgta cacagttatt agtcccacca
       61 gctccaacgg aagaagatgt tatgaagctc gtaagcgtcg ttacccaatt gcttacttta
      121 gttccaccag atcgtcaagc tgctttaata ggtgatttat tcatcccgga atctctaaag
      181 gatatattca atagtttcaa tgaactggcg gcagagaatc gtttacagca aaaaaagagt
      241 gagttggaag gaaggactga agtgaaccat gctaatacaa atgaagaagt tccctccagg
      301 cgaacaagaa gtagagacac aaatgcaaga ggagcatata aattacaaaa caccatcact
      361 gagggcccta aagcggttcc cacgaaaaaa aggagagtag caacgagggt aaggggcaga
      421 aaatcacgta atacttctag ggtatgatcc aatatcaaag gaaatgatag cattgaagga
      481 tgagactaat ccaattgagg agtggcagca tatagaacag ctaaagggta gtgctgaagg
      541 aagcatacga taccccgcat ggaatgggat aatatcacag gaggtactag actacctttc
      601 atcctacata aatagacgca tataagtacg catttaagca taaacacgca ctatgccgtt
      661 cttctcatgt atatatatat acaggcaaca cgcagatata ggtgcgacgt gaacagtgag
      721 ctgtatgtgc gcagctcgcg ttgcattttc ggaagcgctc gttttcggaa acgctttgaa
      781 gttcctattc cgaagttcct attctctaga aagtatagga acttcagagc gcttttgaaa
      841 accaaaagcg ctctgaagac gcactttcaa aaaaccaaaa acgcaccgga ctgtaacgag
      901 ctactaaaat attgcgaata ccgcttccac aaacattgct caaaagtatc tctttgctat
      961 atatctctgt gctatatccc tatataacct acccatccac ctttcgctcc ttgaacttgc
     1021 atctaaactc gacctctaca ttttttatgt ttatctctag tattactctt tagacaaaaa
     1081 aattgtagta agaactattc atagagtgaa tcgaaaacaa tacgaaaatg taaacatttc
     1141 ctatacgtag agaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt
     1201 aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag
     1261 aatagaccga gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga
     1321 acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg
     1381 aaccatcacc ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc
     1441 ctaaagggag cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg
     1501 aagggaagaa agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc
     1561 gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca gggcgcgtgg ggatcgatcc
     1621 actagtccac ggattagaag ccgccgagcg ggtgacagcc ctccgaagga agactctcct
     1681 ccgtgcgtcc tcgtcttcac cggtcgcgtt cctgaaacgc agatgtgcct cgcgccgcac
     1741 tgctccgaac aataaagatt ctacaatact agcttttatg gttatgaaga ggaaaaattg
     1801 gcagtaacct ggccccacaa accttcaaat gaacgaatca aattaacaac cataggatga
     1861 taatgcgatt agttttttag ccttatttct ggggtaatta atcagcgaag cgatgatttt
     1921 tgatctatta acagatatat aaatgcaaaa actgcataac cactttaact aatactttca
     1981 acattttcgg tttgtattac ttcttattca aatgtaataa aagtatcaac aaaaaattgt
     2041 taatatacct ctatacttta acgtcaagga gaaaaaacta taggatcata ggatccatga
     2101 gatttccttc aatttttact gctgatttat tcgcagcatc ctccgcatta gctgctccag
     2161 tcaaaactac aacagaagat gaaacggcac aaattccggc tgaagctgtc atcggttact
     2221 cagatttaga aggggatttc gatgttgctg ttttgccatt ttccaacagc acaaataacg
     2281 gattattgtt tataaatact actattgcca gcattgctgc taaagaagaa ggggtatctc
     2341 tcgagaaaag agagactgaa gctgagttcg cggtaggccc cgtcgcagat ctaaccatca
     2401 cagatgccgt agtgagccct gacggtttct caaggcaagc agtcgtggtc aacggcgtga
     2461 cgcccggacc actaatcgcc ggcaacaagg gcgacagatt ccagttaaat gtaataaaca
     2521 accttacaaa tcacaccatg ctaaaatcta cttctattca ctggcacgga ttcttccagc
     2581 acggaacgaa ttgggccgac ggcccagctt tcataaacca atgcccaata gcaccggggc
     2641 attcatttct atatgatttc caagtacctg accaagcggg taccttttgg tatcattctc
     2701 atctaagcac gcagtactgc gatggattaa ggggcccttt tgttgtgtat gacccaaacg
     2761 acccacacgc tagtatgtat gatgtagata atgacagcac agtaataaca ctggcggact
     2821 ggtaccacac ggctgcaaag ttgggaccca ggtttccacc gggcgcagac gcgactttga
     2881 tcaatggtaa gggcagggca ccgagcgatc cgacagcaga attatccgtc atcaaggtaa
     2941 cgaagggtaa gagataccgt ttcaggttag tctcattgtc atgtgaccct aattttactt
     3001 tctcaataga tggacacaac cttacaatca tagaagtcga tagcgttaac gtgcagcctt
     3061 tagaggtaga ctctatccaa atatttgcag cgcagcgtta ctccttcgtg ttagatgcta
     3121 accaacccgt ggacaattac tggatcagag cgaatcccaa tttcggtaat gtaggattcg
     3181 atggtggtat aaattccgct atcctgaggt atgatggcgc accggccgtg gaaccgacca
     3241 cgaaccaaac aacaagtgtg aagcctttga atgagactga ccttcacccg ttagttccaa
     3301 caccggttcc tggaagtcct tcaccaggtg gcgtagacaa ggccataaac atggccttta
     3361 acttcaacgg gtcaaacttc ttcataaacg gtgtctcctt tgtaccacca acggtacctg
     3421 ttctgctaca gattttaagc ggagctcaga ccgctcagga cttacttccc agtggatcag
     3481 tttacgttct tccctcgaac gcaacaatcg aaatcagttt tcctgcgaca gctgctgcgc
     3541 cgggtgcccc acacccattc cacttgcatg gccatacatt cgctgtggtc cgttcagcag
     3601 gctcaacggt ttacaactac gacaacccca tattcaggga cgtcgtggat acaggtaccc
     3661 cagcagctgg tgacaacgtc actattagat tcgacactaa caaccctggc ccatggttct
     3721 tgcattgcca tgttgacttc catttggaag ctgggtttgc ggtagtaatg gccgaggacc
     3781 tgcccgacgt aaaggctgtg aacccagtcc ctcaggcctg gagtgactta tgcccgactt
     3841 acgatgcctt ggacccaaac gatcagagca gcggtttagt tccccgcggc agcagcgcgc
     3901 accaccacca ccaccactaa gcggccgctc gagcatgcat ctagagggcc gcatcatgta
     3961 attagttatg tcacgcttac attcacgccc tccccccaca tccgctctaa ccgaaaagga
     4021 aggagttaga caacctgaag tctaggtccc tatttatttt tttatagtta tgttagtatt
     4081 gagaacgtta tttatatttc aaatttttct tttttttctg tacagacgcg tgtacgcatg
     4141 taacattata ctgaaaacct tgcttgagaa ggttttggga cgctcgaagg ctttaatttg
     4201 caagctgcgg ccctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
     4261 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
     4321 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
     4381 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     4441 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     4501 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     4561 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     4621 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     4681 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     4741 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     4801 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     4861 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
     4921 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     4981 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     5041 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     5101 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     5161 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
     5221 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
     5281 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
     5341 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
     5401 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
     5461 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
     5521 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
     5581 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
     5641 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
     5701 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
     5761 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
     5821 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
     5881 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
     5941 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
     6001 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
     6061 atactcatac tcttcctttt tcaatgggta ataactgata taattaaatt gaagctctaa
     6121 tttgtgagtt tagtatacat gcatttactt ataatacagt tttttagttt tgctggccgc
     6181 atcttctcaa atatgcttcc cagcctgctt ttctgtaacg ttcaccctct accttagcat
     6241 cccttccctt tgcaaatagt cctcttccaa caataataat gtcagatcct gtagagacca
     6301 catcatccac ggttctatac tgttgaccca atgcgtctcc cttgtcatct aaacccacac
     6361 cgggtgtcat aatcaaccaa tcgtaacctt catctcttcc acccatgtct ctttgagcaa
     6421 taaagccgat aacaaaatct ttgtcgctct tcgcaatgtc aacagtaccc ttagtatatt
     6481 ctccagtaga tagggagccc ttgcatgaca attctgctaa catcaaaagg cctctaggtt
     6541 cctttgttac ttcttctgcc gcctgcttca aaccgctaac aatacctggg cccaccacac
     6601 cgtgtgcatt cgtaatgtct gcccattctg ctattctgta tacacccgca gagtactgca
     6661 atttgactgt attaccaatg tcagcaaatt ttctgtcttc gaagagtaaa aaattgtact
     6721 tggcggataa tgcctttagc ggcttaactg tgccctccat ggaaaaatca gtcaagatat
     6781 ccacatgtgt ttttagtaaa caaattttgg gacctaatgc ttcaactaac tccagtaatt
     6841 ccttggtggt acgaacatcc aatgaagcac acaagtttgt ttgcttttcg tgcatgatat
     6901 taaatagctt ggcagcaaca ggactaggat gagtagcagc acgttcctta tatgtagctt
     6961 tcgacatgat ttatcttcgt ttcctgcagg tttttgttct gtgcagttgg gttaagaata
     7021 ctgggcaatt tcatgtttct tcaacactac atatgcgtat atataccaat ctaagtctgt
     7081 gctccttcct tcgttcttcc ttctgttcgg agattaccga atcaaaaaaa tttcaaggaa
     7141 accgaaatca aaaaaaagaa taaaaaaaaa atgatgaatt gaaaagctag caggtacgta
     7201 gtatatagag acaaaataga agaaaccgtt cataattttc tgaccaatga agaatcatca
     7261 acgctatcac tttctgttca caaagtatgc gcaatccaca tcggtataga atataatcgg
     7321 ggatgccttt atcttgaaaa aatgcacccg cagcttcgct agtaatcagt aaacgcggga
     7381 agtggagtca ggcttttttt atggaagaga aaatagacac caaagtagcc ttcttctaac
     7441 cttaacggac ctacagtgca aaaagttatc aagagactgc attatagagc gcacaaagga
     7501 gaaaaaaagt aatctaagat gctttgttag aaaaatagcg ctctcgggat gcatttttgt
     7561 agaacaaaaa agaagtatag attctttgtt ggtaaaatag cgctctcgcg ttgcatttct
     7621 gttctgtaaa aatgcagctc agattctttg tttgaaaaat tagcgctctc gcgttgcatt
     7681 tttgttttac aaaaatgaag cacagattct tcgttggtaa aatagcgctt tcgcgttgca
     7741 tttctgttct gtaaaaatgc agctcagatt ctttgtttga aaaattagcg ctctcgcgtt
     7801 gcatttttgt tctacaaaat gaagcacaga tgcttcgtta acaaagatat gctattgaag
     7861 tgcaagatgg aaacgcagaa aatgaaccgg ggatgcgacg tgcaagatta cctatgcaat
     7921 agatgcaata gtttctccag gaaccgaaat acatacattg tcttccgtaa agcgctagac
     7981 tatatattat tatacaggtt caaatatact atctgtttca gggaaaactc ccaggttcgg
     8041 atgttcaaaa ttcaatgatg ggtaacaagt acgatcgtaa atctgtaaaa cagtttgtcg
     8101 gatattaggc tgtatctcct caaagcgtat tcgaatatca ttgagaagct gcagcgtcac
     8161 atcggataat aatgatggca gccattgtag aagtgccttt tgcatttcta gtctctttct
     8221 cggtctagct agttttacta catcgcgaag atagaatctt agatcacact gcctttgctg
     8281 agctggatca atagagtaac aaaagagtgg taaggcctcg ttaaaggaca aggacctgag
     8341 cggaagtgta tcgtacagta gacggagtat actagtatag tctatagtcc gtggaattct
     8401 aagtgccagc tttataatgt cattctcctt actacagacc cgcctgaaag tagacacatc
     8461 atcatcagta agctttgaca aaaagcattg agtagctaac tcttctatgc aatctatagc
     8521 tgttttataa ggcattcaat ggacagattg aggtttttga aacatactag tgaaattagc
     8581 cttaatccct tctcgaagtt aatcatgcat tatggtgtaa aaaatgcaac tcgcgttgct
     8641 ctactttttc ccgaatttcc aaatacgcag ctggggtgat tgctcgattt cgtaacgaaa
     8701 gttttgttta taaaaaccgc gaaaaccttc tgtaacagat agatttttac agcgctgata
     8761 tacaatgaca tcagctgtaa tggaaaataa ctgaaatatg aatggcgaga gactgcttgc
     8821 ttgtattaag caatgtatta tgcagcactt ccaacctatg gtgtacgatg aaagtaggtg
     8881 tgtaatcgag acgacaaggg ggacttttcc agttcctgac aattataaga aatacaaaac
     8941 gttagcattt gcatttgttg gacatgtact gaatacagac gacacaccgg taattgaaaa
     9001 agaactggat tggcctgatc ctgcactagt gtacaataca attgtcgatc gaatcataaa
     9061 tcacccagaa ttatcacagt ttatatcggt tgcatttatt agtcagttaa aggccaccat
     9121 cggagagggt ttagatatta atgtaaaagg cacgctaaac cgcaggggaa agggtatcag
     9181 aaggcctaaa ggcgtatttt ttagatacat ggaatctcca tttgtcaata caaaggtcac
     9241 tgcattcttc tcttatcttc gagattataa taaaattgcc tcagaatatc acaataatac
     9301 taaattcatt ctcacgtttt catgtcaagc atattgggca tctggcccaa acttctccgc
     9361 cttgaagaat gttattaggt gctccataat tcatgaatac atttctaagt ttgtggaaag
     9421 agaacaggat aaaggtcata taggagatca ggagctaccg cctgaagagg acccttctcg
     9481 tgaactaaac aatgtacaac atgaagtcaa tagtttaacg gaacaagatg cggaggcgga
     9541 tgaaggattg tggggtgaaa tagattcatt atgtgaaaaa tggcagtctg aagcggaaga
     9601 tcaaactgag gcggagataa tagccgacag gataattgga aatagccaga ggatggcgaa
     9661 cctcaaaatt cgtcgtacaa agttcaaaag tgtcttgtat catatactaa aggaactaat
     9721 tcaatctcag ggaaccgtaa aggtttatcg cggtagtagt ttttcacacg attcgataaa
     9781 gataagctta cattatgaag agcagcatat tacagccgta tgggtctact tgacagtaaa
     9841 atttgaagag cattggaagc ctgttgatgt agaggtcgag tttagatgca agttcaagga
     9901 gcgaaaggtg gatgggtagg ttatataggg atatagcaca gagatatata gcaaagagat
     9961 acttttgagc aatgtttgtg gaagcggtat tcgcaatatt ttagtagctc gttacagtcc
    10021 ggtgcgtttt tggttttttg aaagtgcgtc ttcagagcgc ttttggtttt caaaagcgct
    10081 ctgaagttcc tatactttct agagaatagg aacttcggaa taggaacttc aaagcgtttc
    10141 cgaaaacgag cgcttccgaa aatgcaacgc gagctgcgca catacagctc actgttcacg
    10201 tcgcacctat atctgcgtgt tgcctgtata tatatataca tgagaagaac ggcatagtgc
    10261 gtgtttatgc ttaaatgcgt acttatatgc gtctatttat gtaggatgaa aggtagtcta
    10321 gtacctcctg tgatattatc ccattccatg cggggtatcg tatgcttcct tcagcactac
    10381 cctttagctg ttctatatgc tgccactcct caattggatt agtctcatcc ttcaatgcta
    10441 tcatttcctt tgatattgga tcatatgcat agtaccgaga aactagtgcg aagtagtgat
    10501 caggtattgc tgttatctga tgagtatacg ttgtcctggc cacggcagaa gcacgcttat
    10561 cgctccaatt tcccacaaca ttagtcaact ccgttaggcc cttcattgaa agaaatgagg
    10621 tcatcaaatg tcttccaatg tgagattttg ggccattttt tatagcaaag attgaataag
    10681 gcgcattttt cttcaaagct ttattgtacg atctgactaa gttatctttt aataattggt
    10741 attcctgttt attgcttgaa gaattgccgg tcctatttac tcgttttagg actggttcag
    10801 aattcctcwm wwwttcwtys waatawacaa stggatyrwt mcnnymmsmy wykyrytwwr
    10861 gamkwatttr aatgatttcg gatcaacgtt cttaatatcg ctgaatcttc cacaattgat
    10921 gaaagtagct aggaagagga attggtataa agtttttgtt tttgtaaatc tcgaagtata
    10981 ctcaaacgaa tttagtattt tctcagtgat ctcccagatg ctttcaccct cacttagaag
    11041 tgctttaagc atttttttac tgtggctatt tcccttatct gcttcttccg atgattcgaa
    11101 ctgtaattgc aaactactta caatatcagt gatatcagat tgatgttttt gtccatagta
    11161 aggaataatt gtaaattccc aagcaggaat caatttcttt aatgaggctt ccagaattgt
    11221 tgctttttgc gtcttgtatt taaactggag tgatttattg acaatatcga aactcagcga
    11281 attgcttatg atagtattat agctcatgaa tgtggctctc ttgattgctg ttccgttatg
    11341 tgtaatcatc caacataaat aggttagttc agcagcacat aatgctattt tctcacctga
    11401 aggtctttca aacctttcca caaactgacg aacaagcacc ttaggtggtg ttttacataa
    11461 tatatcaaat tgtggcatgc ttagcgccga tcttgtgtgc aattgatatc tagtttcaac
    11521 tactctattt atcttgtatc ttgcagtatt caaacacgct aactcgaaaa actaacttta
    11581 attgtcctgt ttgtctcgcg ttctttcgaa aaatgcaccg gccgcgcatt atttgtactg
    11641 cgaaaataat tggtactgcg gtatcttcat ttcatatttt aaaaatgcac ctttgctgct
    11701 tttccttaat ttttagacgg cccgcaggtt cgttttgcgg tactatcttg tgataaaaag
    11761 ttgttttgac atgtgatctg cacagatttt ataatgtaat aagcaagaat acattatcaa
    11821 acgaacaata ctggtaaaag aaaaccaaaa tggacgacat tgaaacagcc aagaatctga
    11881 cggtaaaagc acgtacagct tatagcgtct gggatgtatg tcggctgttt attgaaatga
    11941 ttgctcctga tgtagatatt gatatagaga gtaaacgtaa gtctgatgag ctactctttc
    12001 caggatatgt cataaggccc atggaatctc tcacaaccgg taggccgtat ggtcttgatt
    12061 ctagcgcaga agattccagc gtatcttctg actccagtgc tgaggtaatt ttgcctgctg
    12121 cgaagatggt taaggaaagg tttgattcga ttggaaatgg tatgctctct tcacaagaag
    12181 caagtcaggc tgccatagat ttgatgctac agaataacaa gctgttagac aatagaaagc
    12241 aactatacaa atctattgct ataataatag gaagattgcc cgagaaagac aag
//
