LOCUS       sequence-370145-       15622 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  gRNA array of iMAP-61.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15622)
  AUTHORS   Liu B, Jing Z, Zhang X, Chen Y, Mao S, Kaundal R, Zou Y, Wei G, Zang
            Y, Wang X, Lin W, Di M, Sun Y, Chen Q, Li Y, Xia J, Sun J, Lin CP, 
            Huang X, Chi T
  TITLE     Large-scale multiplexed mosaic CRISPR perturbation in the whole 
            organism.
  JOURNAL   Cell. 2022 Aug 4;185(16):3008-3024.e16. doi: 
            10.1016/j.cell.2022.06.039. Epub 2022 Jul 22.
  PUBMED    35870449
REFERENCE   2  (bases 1 to 15622)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.06.039"; journalName: "Cell"; date: "2022-08-4-
            4"; volume: "185"; issue: "16"; pages: "3008-3024.e16"
COMMENT     Sequence Label: iMAP-61
FEATURES             Location/Qualifiers
     source          1..15622
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(108..899,900..968))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     731..750
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(969..1073)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1141..1159
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     repeat_region   1175..1209
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     1759..1779
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
     primer_bind     complement(11895..11917)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    11906..11922
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     intron          12527..13544
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     13468..13490
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     13552..13571
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      13600..13609
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             13606..14316
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYDG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(13751..13769)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     14019..14038
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     polyA_signal    14337..14471
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(14361..14380)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     14415..14434
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     repeat_region   14749..14811
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     rep_origin      complement(14971..15559)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(15051..15070)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
       61 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
      121 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
      181 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
      241 ataccgcgag atccacgctc accggctcca gatttatcag caataaacca gccagccgga
      301 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
      361 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
      421 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
      481 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
      541 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
      601 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
      661 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
      721 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
      781 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
      841 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
      901 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
      961 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
     1021 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
     1081 ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa
     1141 aataggcgta tcacgaggcc ctttaggcct ttaaccctag aaagatagtc tgcataaaat
     1201 tgacgcatgc attcttgaaa tattgctctc tctttctaaa tagcgcgaat ccgtcgctgt
     1261 gcatttagga catctcagtc gccgcttgga gctcccgtga ggcgtgcttg tcaatgcggt
     1321 aagtgtcact gattttgaac tataacgacc gcgtgagtca aaatgacgca tgattatctt
     1381 ttacgtgact tttaagattt aactcatacg ataattatat tgttatttca tgttctactt
     1441 acgtgataac ttattatata tatattttct tgttatagat agcttcgata ccgtcggctc
     1501 gagaatgcat ctagaggatc cccacaggtc cgacgccgcc atctctaggc ccgcgccggc
     1561 cccctcgcac agacttgtgg gagaagctcg gctactcccc tgccccggtt aatttgcata
     1621 taatatttcc tagtaactat agaggcttaa tgtgcgataa aagacagata atctgttctt
     1681 tttaatacta gctacatttt acatgatagg cttggatttc tataagagat acaaatacta
     1741 aattattatt ttaaaaaaca gcacaaaagg aaactcaccc taactgtaaa gtaatttacc
     1801 gttcgtatag tataaattat acgaagttat aagccttgtt tgatcagcct gttcttacga
     1861 gggtttaaga gctatgctgg aaacagcata gcaagtttaa ataaggctag tccgttatca
     1921 acttgaaaaa gtggcaccga gtcggtgctt tttttggatc cagatataac ttcgtatagt
     1981 ataaattatt gcttcggtaa agccttgttt gatagtggga accttctggg agtttaagag
     2041 ctatgctgga aacagcatag caagtttaaa taaggctagt ccgttatcaa cttgaaaaag
     2101 tggcaccgag tcggtgcttt ttttgggact ataacttcgt atagtataaa ttattgcttc
     2161 ggtaaagcct tgtttgaatg tctcagacca tatggggttt aagagctatg ctggaaacag
     2221 catagcaagt ttaaataagg ctagtccgtt atcaacttga aaaagtggca ccgagtcggt
     2281 gctttttttg ggactataac ttcgtatagt ataaattatt gcttcggtaa agccttgttt
     2341 gtgtctcgaa caactacact ggtttaagag ctatgctgga aacagcatag caagtttaaa
     2401 taaggctagt ccgttatcaa cttgaaaaag tggcaccgag tcggtgcttt ttttgggact
     2461 ataacttcgt atagtataaa ttattgcttc ggtaaagcct tgtttgtgaa atatgatgca
     2521 actgtggttt aagagctatg ctggaaacag catagcaagt ttaaataagg ctagtccgtt
     2581 atcaacttga aaaagtggca ccgagtcggt gctttttttg ctattataac ttcgtatagt
     2641 ataaattatt gcttcggtaa agccttgttt gactattcca atgttcagtg ggtttaagag
     2701 ctatgctgga aacagcatag caagtttaaa taaggctagt ccgttatcaa cttgaaaaag
     2761 tggcaccgag tcggtgcttt ttttgggact ataacttcgt atagtataaa ttattgcttc
     2821 ggtaaagcct tgtttgagtg cttgcaaggc ccagaggttt aagagctatg ctggaaacag
     2881 catagcaagt ttaaataagg ctagtccgtt atcaacttga aaaagtggca ccgagtcggt
     2941 gctttttttt acgtataact tcgtatagta taaattattg cttcggtaaa gccttgtttg
     3001 cttaccagga tatcttctgg gtttaagagc tatgctggaa acagcatagc aagtttaaat
     3061 aaggctagtc cgttatcaac ttgaaaaagt ggcaccgagt cggtgctttt tttgctatta
     3121 taacttcgta tagtataaat tattgcttcg gtaaagcctt gtttgaatca gagtctaatc
     3181 aaccagttta agagctatgc tggaaacagc atagcaagtt taaataaggc tagtccgtta
     3241 tcaacttgaa aaagtggcac cgagtcggtg ctttttttgc tgctataact tcgtatagta
     3301 taaattattg cttcggtaaa gccttgtttg ttgttcgaga cacaacagcg gtttaagagc
     3361 tatgctggaa acagcatagc aagtttaaat aaggctagtc cgttatcaac ttgaaaaagt
     3421 ggcaccgagt cggtgctttt tttgctatta taacttcgta tagtataaat tattgcttcg
     3481 gtaaagcctt gtttgtgacc tgcatcccat caaaggttta agagctatgc tggaaacagc
     3541 atagcaagtt taaataaggc tagtccgtta tcaacttgaa aaagtggcac cgagtcggtg
     3601 ctttttttgc tgctataact tcgtatagta taaattattg cttcggtaaa gccttgtttg
     3661 gagtcacaat tcccagtcag gtttaagagc tatgctggaa acagcatagc aagtttaaat
     3721 aaggctagtc cgttatcaac ttgaaaaagt ggcaccgagt cggtgctttt tttgctatta
     3781 taacttcgta tagtataaat tattgcttcg gtaaagcctt gtttgtaaat gtgcagacag
     3841 atttggttta agagctatgc tggaaacagc atagcaagtt taaataaggc tagtccgtta
     3901 tcaacttgaa aaagtggcac cgagtcggtg ctttttttcg aatataactt cgtatagtat
     3961 aaattattgc ttcggtaaag ccttgtttgg aagggacaaa agatgacagg tttaagagct
     4021 atgctggaaa cagcatagca agtttaaata aggctagtcc gttatcaact tgaaaaagtg
     4081 gcaccgagtc ggtgcttttt ttgggactat aacttcgtat agtataaatt attgcttcgg
     4141 taaagccttg tttgaatcac tagcaagatg cccagtttaa gagctatgct ggaaacagca
     4201 tagcaagttt aaataaggct agtccgttat caacttgaaa aagtggcacc gagtcggtgc
     4261 tttttttgct gctataactt cgtatagtat aaattattgc ttcggtaaag ccttgtttgt
     4321 gtctcgaaca actacactgg tttaagagct atgctggaaa cagcatagca agtttaaata
     4381 aggctagtcc gttatcaact tgaaaaagtg gcaccgagtc ggtgcttttt ttgctgctat
     4441 aacttcgtat agtataaatt attgcttcgg taaagccttg tttggattat ggtcctgaga
     4501 gccggtttaa gagctatgct ggaaacagca tagcaagttt aaataaggct agtccgttat
     4561 caacttgaaa aagtggcacc gagtcggtgc tttttttgct gctataactt cgtatagtat
     4621 aaattattgc ttcggtaaag ccttgtttga aacaaaagga gatatcaagg tttaagagct
     4681 atgctggaaa cagcatagca agtttaaata aggctagtcc gttatcaact tgaaaaagtg
     4741 gcaccgagtc ggtgcttttt ttgctgctat aacttcgtat agtataaatt attgcttcgg
     4801 taaagccttg tttgaaatgc taagtgtgga aatggtttaa gagctatgct ggaaacagca
     4861 tagcaagttt aaataaggct agtccgttat caacttgaaa aagtggcacc gagtcggtgc
     4921 tttttttctc gtataacttc gtatagtata aattattgct tcggtaaagc cttgtttgga
     4981 cttttcgcca cagcgtgggt ttaagagcta tgctggaaac agcatagcaa gtttaaataa
     5041 ggctagtccg ttatcaactt gaaaaagtgg caccgagtcg gtgctttttt tgctattata
     5101 acttcgtata gtataaatta ttgcttcggt aaagccttgt ttggaggaaa cctgaccatc
     5161 gaggtttaag agctatgctg gaaacagcat agcaagttta aataaggcta gtccgttatc
     5221 aacttgaaaa agtggcaccg agtcggtgct ttttttgctg ctataacttc gtatagtata
     5281 aattattgct tcggtaaagc cttgtttggc actctggaca gttccacagt ttaagagcta
     5341 tgctggaaac agcatagcaa gtttaaataa ggctagtccg ttatcaactt gaaaaagtgg
     5401 caccgagtcg gtgctttttt tgctgctata acttcgtata gtataaatta ttgcttcggt
     5461 aaagccttgt ttggacaggt cgattgctct ggggtttaag agctatgctg gaaacagcat
     5521 agcaagttta aataaggcta gtccgttatc aacttgaaaa agtggcaccg agtcggtgct
     5581 ttttttgctg ctataacttc gtatagtata aattattgct tcggtaaagc cttgtttgtg
     5641 tgcatattta ttgcatcggt ttaagagcta tgctggaaac agcatagcaa gtttaaataa
     5701 ggctagtccg ttatcaactt gaaaaagtgg caccgagtcg gtgctttttt tgctgctata
     5761 acttcgtata gtataaatta ttgcttcggt aaagccttgt ttgtgtaatt tgtttgggca
     5821 cgagtttaag agctatgctg gaaacagcat agcaagttta aataaggcta gtccgttatc
     5881 aacttgaaaa agtggcaccg agtcggtgct ttttttttca tataacttcg tatagtataa
     5941 attattgctt cggtaaagcc ttgtttgcat aaggtaccac cacgctggtt taagagctat
     6001 gctggaaaca gcatagcaag tttaaataag gctagtccgt tatcaacttg aaaaagtggc
     6061 accgagtcgg tgcttttttt gctgctataa cttcgtatag tataaattat tgcttcggta
     6121 aagccttgtt tgattcaaac tgctcccccg aggtttaaga gctatgctgg aaacagcata
     6181 gcaagtttaa ataaggctag tccgttatca acttgaaaaa gtggcaccga gtcggtgctt
     6241 tttttgctgc tataacttcg tatagtataa attattgctt cggtaaagcc ttgtttggta
     6301 accaatagct tccaatggtt taagagctat gctggaaaca gcatagcaag tttaaataag
     6361 gctagtccgt tatcaacttg aaaaagtggc accgagtcgg tgcttttttt gggactataa
     6421 cttcgtatag tataaattat tgcttcggta aagccttgtt tgttcaaccg aaaagtagac
     6481 cagtttaaga gctatgctgg aaacagcata gcaagtttaa ataaggctag tccgttatca
     6541 acttgaaaaa gtggcaccga gtcggtgctt tttttgctat tataacttcg tatagtataa
     6601 attattgctt cggtaaagcc ttgtttggaa actattccaa tgttcaggtt taagagctat
     6661 gctggaaaca gcatagcaag tttaaataag gctagtccgt tatcaacttg aaaaagtggc
     6721 accgagtcgg tgcttttttt gggactataa cttcgtatag tataaattat tgcttcggta
     6781 aagccttgtt tggaccctcc aagcacaacc atgtttaaga gctatgctgg aaacagcata
     6841 gcaagtttaa ataaggctag tccgttatca acttgaaaaa gtggcaccga gtcggtgctt
     6901 tttttaggtt ataacttcgt atagtataaa ttattgcttc ggtaaagcct tgtttgaatt
     6961 ttcttcttct gtacgggttt aagagctatg ctggaaacag catagcaagt ttaaataagg
     7021 ctagtccgtt atcaacttga aaaagtggca ccgagtcggt gctttttttg ggactataac
     7081 ttcgtatagt ataaattatt gcttcggtaa agccttgttt gaagcctgta tgtgcccctc
     7141 ggtttaagag ctatgctgga aacagcatag caagtttaaa taaggctagt ccgttatcaa
     7201 cttgaaaaag tggcaccgag tcggtgcttt ttttgtgttt ataacttcgt atagtataaa
     7261 ttattgcttc ggtaaagcct tgtttgaatg aaaagtgaca ttgatggttt aagagctatg
     7321 ctggaaacag catagcaagt ttaaataagg ctagtccgtt atcaacttga aaaagtggca
     7381 ccgagtcggt gctttttttg ggactataac ttcgtatagt ataaattatt gcttcggtaa
     7441 agccttgttt gaaataccca gaaacaacgg agtttaagag ctatgctgga aacagcatag
     7501 caagtttaaa taaggctagt ccgttatcaa cttgaaaaag tggcaccgag tcggtgcttt
     7561 ttttgctgct ataacttcgt atagtataaa ttattgcttc ggtaaagcct tgtttgcaag
     7621 ttccgccact gaacatgttt aagagctatg ctggaaacag catagcaagt ttaaataagg
     7681 ctagtccgtt atcaacttga aaaagtggca ccgagtcggt gctttttttg ctgctataac
     7741 ttcgtatagt ataaattatt gcttcggtaa agccttgttt gcatacctga aagatgtcag
     7801 tgtttaagag ctatgctgga aacagcatag caagtttaaa taaggctagt ccgttatcaa
     7861 cttgaaaaag tggcaccgag tcggtgcttt ttttgcagta taacttcgta tagtataaat
     7921 tattgcttcg gtaaagcctt gtttgaacag atcgtccatg cagtggttta agagctatgc
     7981 tggaaacagc atagcaagtt taaataaggc tagtccgtta tcaacttgaa aaagtggcac
     8041 cgagtcggtg ctttttttgc tgctataact tcgtatagta taaattattg cttcggtaaa
     8101 gccttgtttg gcacccggtc agagagatgt gtttaagagc tatgctggaa acagcatagc
     8161 aagtttaaat aaggctagtc cgttatcaac ttgaaaaagt ggcaccgagt cggtgctttt
     8221 tttgctgcta taacttcgta tagtataaat tattgcttcg gtaaagcctt gtttgccacc
     8281 ctggtcatta tggaagttta agagctatgc tggaaacagc atagcaagtt taaataaggc
     8341 tagtccgtta tcaacttgaa aaagtggcac cgagtcggtg ctttttttgg gactataact
     8401 tcgtatagta taaattattg cttcggtaaa gccttgtttg tgggaccttg ccatcccacg
     8461 gtttaagagc tatgctggaa acagcatagc aagtttaaat aaggctagtc cgttatcaac
     8521 ttgaaaaagt ggcaccgagt cggtgctttt tttgctatta taacttcgta tagtataaat
     8581 tattgcttcg gtaaagcctt gtttgtatac atagcgcctc tgactgttta agagctatgc
     8641 tggaaacagc atagcaagtt taaataaggc tagtccgtta tcaacttgaa aaagtggcac
     8701 cgagtcggtg ctttttttgt gtttataact tcgtatagta taaattattg cttcggtaaa
     8761 gccttgtttg gtagcagaaa tcttatatcg gtttaagagc tatgctggaa acagcatagc
     8821 aagtttaaat aaggctagtc cgttatcaac ttgaaaaagt ggcaccgagt cggtgctttt
     8881 tttgatatat aacttcgtat agtataaatt attgcttcgg taaagccttg tttgtcccca
     8941 gcatcttatc cggggtttaa gagctatgct ggaaacagca tagcaagttt aaataaggct
     9001 agtccgttat caacttgaaa aagtggcacc gagtcggtgc tttttttggg actataactt
     9061 cgtatagtat aaattattgc ttcggtaaag ccttgtttgt ttagcccaca catacagctg
     9121 tttaagagct atgctggaaa cagcatagca agtttaaata aggctagtcc gttatcaact
     9181 tgaaaaagtg gcaccgagtc ggtgcttttt ttgctgctat aacttcgtat agtataaatt
     9241 attgcttcgg taaagccttg tttgcaggta gacatcgttg atgggtttaa gagctatgct
     9301 ggaaacagca tagcaagttt aaataaggct agtccgttat caacttgaaa aagtggcacc
     9361 gagtcggtgc tttttttgct attataactt cgtatagtat aaattattgc ttcggtaaag
     9421 ccttgtttgt gaagttgtta tattcagagg tttaagagct atgctggaaa cagcatagca
     9481 agtttaaata aggctagtcc gttatcaact tgaaaaagtg gcaccgagtc ggtgcttttt
     9541 ttgctgctat aacttcgtat agtataaatt attgcttcgg taaagccttg tttgaattca
     9601 ctgtaaagct ggaagtttaa gagctatgct ggaaacagca tagcaagttt aaataaggct
     9661 agtccgttat caacttgaaa aagtggcacc gagtcggtgc tttttttggg actataactt
     9721 cgtatagtat aaattattgc ttcggtaaag ccttgtttga ccacatgttt gcttcgttgg
     9781 tttaagagct atgctggaaa cagcatagca agtttaaata aggctagtcc gttatcaact
     9841 tgaaaaagtg gcaccgagtc ggtgcttttt tttcgatata acttcgtata gtataaatta
     9901 ttgcttcggt aaagccttgt ttgagaaggg acaaaagatg acagtttaag agctatgctg
     9961 gaaacagcat agcaagttta aataaggcta gtccgttatc aacttgaaaa agtggcaccg
    10021 agtcggtgct ttttttgcta ttataacttc gtatagtata aattattgct tcggtaaagc
    10081 cttgtttgca atgtctcaga ccatatgggt ttaagagcta tgctggaaac agcatagcaa
    10141 gtttaaataa ggctagtccg ttatcaactt gaaaaagtgg caccgagtcg gtgctttttt
    10201 tgggactata acttcgtata gtataaatta ttgcttcggt aaagccttgt ttgcagccgt
    10261 tccataatga ccagtttaag agctatgctg gaaacagcat agcaagttta aataaggcta
    10321 gtccgttatc aacttgaaaa agtggcaccg agtcggtgct ttttttgcta ttataacttc
    10381 gtatagtata aattattgct tcggtaaagc cttgtttgca tgtctatgac ctgtgcaagt
    10441 ttaagagcta tgctggaaac agcatagcaa gtttaaataa ggctagtccg ttatcaactt
    10501 gaaaaagtgg caccgagtcg gtgctttttt tgggactata acttcgtata gtataaatta
    10561 ttgcttcggt aaagccttgt ttgttggtca agatcttcac agagtttaag agctatgctg
    10621 gaaacagcat agcaagttta aataaggcta gtccgttatc aacttgaaaa agtggcaccg
    10681 agtcggtgct ttttttgctg ctataacttc gtatagtata aattattgct tcggtaaagc
    10741 cttgtttgag aaattgacat tcacctgggt ttaagagcta tgctggaaac agcatagcaa
    10801 gtttaaataa ggctagtccg ttatcaactt gaaaaagtgg caccgagtcg gtgctttttt
    10861 ttggctataa cttcgtatag tataaattat tgcttcggta aagccttgtt tgtctacaga
    10921 tgactgccat gggtttaaga gctatgctgg aaacagcata gcaagtttaa ataaggctag
    10981 tccgttatca acttgaaaaa gtggcaccga gtcggtgctt tttttgctgc tataacttcg
    11041 tatagtataa attattgctt cggtaaagcc ttgtttgcac gtgcaatccc aggccaggtt
    11101 taagagctat gctggaaaca gcatagcaag tttaaataag gctagtccgt tatcaacttg
    11161 aaaaagtggc accgagtcgg tgcttttttt gggactataa cttcgtatag tataaattat
    11221 tgcttcggta aagccttgtt tggacagttc cacaaggaac gtgtttaaga gctatgctgg
    11281 aaacagcata gcaagtttaa ataaggctag tccgttatca acttgaaaaa gtggcaccga
    11341 gtcggtgctt tttttgctgc tataacttcg tatagtataa attattgctt cggtaaagcc
    11401 ttgtttgatg ttggcagcaa tggagcggtt taagagctat gctggaaaca gcatagcaag
    11461 tttaaataag gctagtccgt tatcaacttg aaaaagtggc accgagtcgg tgcttttttt
    11521 gctattataa cttcgtatag tataaattat tgcttcggta aagccttgtt tgtggtcaag
    11581 atcttcacag aagtttaaga gctatgctgg aaacagcata gcaagtttaa ataaggctag
    11641 tccgttatca acttgaaaaa gtggcaccga gtcggtgctt tttttgctgc tataacttcg
    11701 tatagtataa attattgctt cggtaaagcc ttgtttgact cttacaccat ccactctgtt
    11761 taagagctat gctggaaaca gcatagcaag tttaaataag gctagtccgt tatcaacttg
    11821 aaaaagtggc accgagtcgg tgctttttta acaagcttta ccgttcgtat agtataaatt
    11881 atacgaagtt atttcctgtg tgaaattgtt atccgctcac aattacacac aacatacgag
    11941 ccggaatgca taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc
    12001 attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg
    12061 tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat
    12121 gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca
    12181 gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat
    12241 taccatggtc gaggtgagcc ccacgttctg cttcactctc cccatctccc ccccctcccc
    12301 acccccaatt ttgtatttat ttatttttta attattttgt gcagcgatgg gggcgggggg
    12361 gggggggggg cgcgcgccrg gsggggsggg gsggggsggg gggsggggsg gggsgrggsg
    12421 grgaggtgcg gcggcagcca atcagagcgg cgcgctccga aagtttcctt ttatggcgag
    12481 gcggcggcgg cggcggccct ataaaaagcg aagcgcgcgg cgggcgggag tcgctgcgcg
    12541 ctgccttcgc cccgtgcccc gctccgccgc cgcctcgcgc cgcccgcccc ggctctgact
    12601 gaccgcgtta ctcccacagg tgagcgggcg ggacggccct tctcctccgg gctgtaatta
    12661 gcgcttggtt taatgacggc ttgtttcttt tctgtggctg cgtgaaagcc ttgaggggct
    12721 ccgggagggc cctttgtgcg gggggagcgg ctcggggggt gcgtgcgtgt gtgtgtgtgc
    12781 gtggggagcg ccgcgtgcgg ctccgcgctg cccggcggct gtgagcgctg cgggcgcggc
    12841 gcggggcttt gtgcgctccg cagtgtgcgc gaggggagcg cggccggggg cggtgccccg
    12901 cggtgcgggg ggggctgcga ggggaacaaa ggctgcgtgc ggggtgtgtg cgtggggggg
    12961 tgagcagggg gtgtgggcgc gtcggtcggg ctgcaacccc cctgcacccc cctccccgag
    13021 ttgctgagca cggcccggct tcgggtgcgg ggctccgtac ggggcgtggc gcggggctcg
    13081 ccgtgccggg cggggggtgg cggcaggtgg gggtgccggg cggggcgggg ccgcctcggg
    13141 ccggggrggg ctcgggggrg gggcgcggcg gcccccggag cgccggcggc tgtcgaggcg
    13201 cggcgagccg cagccattgc cttttatggt aatcgtgcga gagggcgcag ggacttcctt
    13261 tgtcccaaat ctgtgcggag ccgaaatctg ggaggcgccg ccgcaccccc tctagcgggc
    13321 gcggggcgaa gcggtgcggc gccggcagga aggaaatggg cggggagggc cttcgtgcgt
    13381 cgccgcgccg ccgtcccctt ctccctctcc agcctcgggg ctgtccgcgg ggggacggct
    13441 gccttcgggg gggacggggc agggcggggt tcggcttctg gcgtgtgacc ggcggctcta
    13501 gagcctctgc taaccatgtt catgccttct tctttttcct acagctcctg ggcaacgtgc
    13561 tggttattgt gctgtctcat cattttggca aagaattggg ccaccatggt gagcaagggc
    13621 gaggaggata acatggccat catcaaggag ttcatgcgct tcaaggtgca catggagggc
    13681 tccgtgaacg gccacgagtt cgagatcgag ggcgagggcg agggccgccc ctacgatggc
    13741 acccagaccg ccaagctgaa ggtgaccaag ggtggccccc tgcccttcgc ctgggacatc
    13801 ctgtcccctc agttcatgta cggctccaag gcctacgtga agcaccccgc cgacatcccc
    13861 gactacttga agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag
    13921 gacggcggcg tggtgaccgt gacccaggac tcctccctgc aggacggcga gttcatctac
    13981 aaggtgaagc tgcgcggcac caacttcccc tccgacggcc ccgtaatgca gaagaagacc
    14041 atgggctggg aggcctcctc cgagcggatg taccccgagg acggcgccct gaagggcgag
    14101 atcaagcaga ggctgaagct gaaggacggc ggccactacg acgctgaggt caagaccacc
    14161 tacaaggcca agaagcccgt gcagctgccc ggcgcctaca acgtcaacat caagttggac
    14221 atcacctccc acaacgagga ctacaccatc gtggaacagt acgaacgcgc cgagggccgc
    14281 cactccaccg gcggcatgga cgagctgtac aagtaaggcg cgccataatc gatgatgatc
    14341 cagacatgat aagatacatt gatgagtttg gacaaaccac aactagaatg cagtgaaaaa
    14401 aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt ataagctgca
    14461 ataaacaagt taacaacaac aattgcattc attttatgtt tcaggttcag ggggaggtgt
    14521 gggaggtttt ttataccgaa tgcatctaga ggatcctcga gcccgtcgac cgataaaagt
    14581 tttgttactt tatagaagaa attttgagtt tttgtttttt tttaataaat aaataaacat
    14641 aaataaattg tttgttgaat ttattattag tatgtaagtg taaatataat aaaacttaat
    14701 atctattcaa attaataaat aaacctcgat atacagaccg ataaaacaca tgcgtcaatt
    14761 ttacgcatga ttatctttaa cgtacgtcac aatatgatta tctttctagg gttaaaggcc
    14821 ttcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
    14881 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
    14941 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
    15001 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
    15061 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
    15121 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
    15181 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
    15241 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
    15301 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
    15361 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
    15421 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
    15481 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
    15541 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
    15601 gaacgaaaac tcacgttaag gg
//
