LOCUS       sequence-370147-       11783 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  PROSS-designed versatile peroxidase 8H.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11783)
  AUTHORS   Barber-Zucker S, Mindel V, Garcia-Ruiz E, Weinstein JJ, Alcalde M, 
            Fleishman SJ
  TITLE     Stable and Functionally Diverse Versatile Peroxidases Designed 
            Directly from Sequences.
  JOURNAL   J Am Chem Soc. 2022 Mar 2;144(8):3564-3571. doi: 
            10.1021/jacs.1c12433. Epub 2022 Feb 18.
  PUBMED    35179866
REFERENCE   2  (bases 1 to 11783)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/jacs.1c12433"; journalName: "J Am Chem Soc"; date: 
            "2022-03-2- 2"; volume: "144"; issue: "8"; pages: "3564-3571"
COMMENT     Sequence Label: pJRoC30_VP8H
FEATURES             Location/Qualifiers
     source          1..11783
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(1..48)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     protein_bind    2989..3036
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     rep_origin      complement(3364..3819)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3501..3522)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3713..3732
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3841..4282
                     /gene="S. cerevisiae GAL1"
                     /label=GAL1 promoter
                     /note="inducible promoter, regulated by Gal4"
     protein_bind    3841..3958
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     primer_bind     4253..4276
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     CDS             join(4308..4364,4365..4562,4563..4574)
                     /codon_start=1
                     /gene="MF-alpha-1"
                     /product="N-terminal secretion signal from S. cerevisiae 
                     alpha-factor"
                     /label=alpha-factor secretion signal
                     /note="Cleavage by the Kex2 protease occurs after the 
                     dibasic KR sequence. The EA dipeptides are then removed by 
                     dipeptidyl aminopeptidase A."
                     /translation="MRFPSIFTAVLFAASSALAAPVNTTTEDETAQIPAEAVIGYSDLE
                     GDFDVAVLPFSNSTNNGLLFINTTIASIAAKEEGVSLEKREAEA"
     terminator      5655..5902
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(5671..5689)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     primer_bind     complement(5979..5996)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6150..6738)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6230..6249)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6909..7700,7701..7769))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7532..7551
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     CDS             complement(7865..8668)
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     promoter        complement(8669..8884)
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
ORIGIN
        1 gaagttccta tactttctag agaataggaa cttcggaata ggaacttcaa agcgtttccg
       61 aaaacgagcg cttccgaaaa tgcaacgcga gctgcgcaca tacagctcac tgttcacgtc
      121 gcacctatat ctgcgtgttg cctgtatata tatatacatg agaagaacgg catagtgcgt
      181 gtttatgctt aaatgcgtac ttatatgcgt ctatttatgt aggatgaaag gtagtctagt
      241 acctcctgtg atattatccc attccatgcg gggtatcgta tgcttccttc agcactaccc
      301 tttagctgtt ctatatgctg ccactcctca attggattag tctcatcctt caatgctatc
      361 atttcctttg atattggatc atatgcatag taccgagaaa ctagtgcgaa gtagtgatca
      421 ggtattgctg ttatctgatg agtatacgtt gtcctggcca cggcagaagc acgcttatcg
      481 ctccaatttc ccacaacatt agtcaactcc gttaggccct tcattgaaag aaatgaggtc
      541 atcaaatgtc ttccaatgtg agattttggg ccatttttta tagcaaagat tgaataaggc
      601 gcatttttct tcaaagcttt attgtacgat ctgactaagt tatcttttaa taattggtat
      661 tcctgtttat tgcttgaaga attgccggtc ctatttactc gttttaggac tggttcagaa
      721 tkcctswmww wttcwtyswa atawacaast ggatyrwtmc nnymmsmywy kyrytwwrga
      781 mkwatttrwr ysatttcgga tcaacgttct taatatcgct gaatcttcca caattgatga
      841 aagtagctag gaagaggaat tggtataaag tttttgtttt tgtaaatctc gaagtatact
      901 caaacgaatt tagtattttc tcagtgatct cccagatgct ttcaccctca cttagaagtg
      961 ctttaagcat ttttttactg tggctatttc ccttatctgc ttcttccgat gattcgaact
     1021 gtaattgcaa actacttaca atatcagtga tatcagattg atgtttttgt ccatagtaag
     1081 gaataattgt aaattcccaa gcaggaatca atttctttaa tgaggcttcc agaattgttg
     1141 ctttttgcgt cttgtattta aactggagtg atttattgac aatatcgaaa ctcagcgaat
     1201 tgcttatgat agtattatag ctcatgaatg tggctctctt gattgctgtt ccgttatgtg
     1261 taatcatcca acataaatag gttagttcag cagcacataa tgctattttc tcacctgaag
     1321 gtctttcaaa cctttccaca aactgacgaa caagcacctt aggtggtgtt ttacataata
     1381 tatcaaattg tggcatgctt agcgccgatc ttgtgtgcaa ttgatatcta gtttcaacta
     1441 ctctatttat cttgtatctt gcagtattca aacacgctaa ctcgaaaaac taactttaat
     1501 tgtcctgttt gtctcgcgtt ctttcgaaaa atgcaccggc cgcgcattat ttgtactgcg
     1561 aaaataattg gtactgcggt atcttcattt catattttaa aaatgcacct ttgctgcttt
     1621 tccttaattt ttagacggcc cgcaggttcg ttttgcggta ctatcttgtg ataaaaagtt
     1681 gttttgacat gtgatctgca cagattttat aatgtaataa gcaagaatac attatcaaac
     1741 gaacaatact ggtaaaagaa aaccaaaatg gacgacattg aaacagccaa gaatctgacg
     1801 gtaaaagcac gtacagctta tagcgtctgg gatgtatgtc ggctgtttat tgaaatgatt
     1861 gctcctgatg tagatattga tatagagagt aaacgtaagt ctgatgagct actctttcca
     1921 ggatatgtca taaggcccat ggaatctctc acaaccggta ggccgtatgg tcttgattct
     1981 agcgcagaag attccagcgt atcttctgac tccagtgctg aggtaatttt gcctgctgcg
     2041 aagatggtta aggaaaggtt tgattcgatt ggaaatggta tgctctcttc acaagaagca
     2101 agtcaggctg ccatagattt gatgctacag aataacaagc tgttagacaa tagaaagcaa
     2161 ctatacaaat ctattgctat aataatagga agattgcccg agaaagacaa gaagagagct
     2221 accgaaatgc tcatgagaaa aatggattgt acacagttat tagtcccacc agctccaacg
     2281 gaagaagatg ttatgaagct cgtaagcgtc gttacccaat tgcttacttt agttccacca
     2341 gatcgtcaag ctgctttaat aggtgattta ttcatcccgg aatctctaaa ggatatattc
     2401 aatagtttca atgaactggc ggcagagaat cgtttacagc aaaaaaagag tgagttggaa
     2461 ggaaggactg aagtgaacca tgctaataca aatgaagaag ttccctccag gcgaacaaga
     2521 agtagagaca caaatgcaag aggagcatat aaattacaaa acaccatcac tgagggccct
     2581 aaagcggttc ccacgaaaaa aaggagagta gcaacgaggg taaggggcag aaaatcacgt
     2641 aatacttcta gggtatgatc caatatcaaa ggaaatgata gcattgaagg atgagactaa
     2701 tccaattgag gagtggcagc atatagaaca gctaaagggt agtgctgaag gaagcatacg
     2761 ataccccgca tggaatggga taatatcaca ggaggtacta gactaccttt catcctacat
     2821 aaatagacgc atataagtac gcatttaagc ataaacacgc actatgccgt tcttctcatg
     2881 tatatatata tacaggcaac acgcagatat aggtgcgacg tgaacagtga gctgtatgtg
     2941 cgcagctcgc gttgcatttt cggaagcgct cgttttcgga aacgctttga agttcctatt
     3001 ccgaagttcc tattctctag aaagtatagg aacttcagag cgcttttgaa aaccaaaagc
     3061 gctctgaaga cgcactttca aaaaaccaaa aacgcaccgg actgtaacga gctactaaaa
     3121 tattgcgaat accgcttcca caaacattgc tcaaaagtat ctctttgcta tatatctctg
     3181 tgctatatcc ctatataacc tacccatcca cctttcgctc cttgaacttg catctaaact
     3241 cgacctctac attttttatg tttatctcta gtattactct ttagacaaaa aaattgtagt
     3301 aagaactatt catagagtga atcgaaaaca atacgaaaat gtaaacattt cctatacgta
     3361 gagaaattgt aagcgttaat attttgttaa aattcgcgtt aaatttttgt taaatcagct
     3421 cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa gaatagaccg
     3481 agatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaag aacgtggact
     3541 ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg cccactacgt gaaccatcac
     3601 cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga
     3661 gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga
     3721 aagcgaaagg agcgggcgct agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca
     3781 ccacacccgc cgcgcttaat gcgccgctac agggcgcgtg gggatcgatc cactagtcca
     3841 cggattagaa gccgccgagc gggtgacagc cctccgaagg aagactctcc tccgtgcgtc
     3901 ctcgtcttca ccggtcgcgt tcctgaaacg cagatgtgcc tcgcgccgca ctgctccgaa
     3961 caataaagat tctacaatac tagcttttat ggttatgaag aggaaaaatt ggcagtaacc
     4021 tggccccaca aaccttcaaa tgaacgaatc aaattaacaa ccataggatg ataatgcgat
     4081 tagtttttta gccttatttc tggggtaatt aatcagcgaa gcgatgattt ttgatctatt
     4141 aacagatata taaatgcaaa aactgcataa ccactttaac taatactttc aacattttcg
     4201 gtttgtatta cttcttattc aaatgtaata aaagtatcaa caaaaaattg ttaatatacc
     4261 tctatacttt aacgtcaagg agaaaaaact ataggatcat aggatccatg agatttcctt
     4321 caatttttac tgctgtttta ttcgcagcat cctccgcatt agctgctcca gtcaacacta
     4381 caacagaaga tgaaacggca caaattccgg ctgaagctgt catcggttac tcagatttag
     4441 aaggggattt cgatgttgct gttttgccat tttccaacag cacaaataac gggttattgt
     4501 ttataaatac tactattgcc agcattgctg ctaaagaaga aggggtatct ctcgagaaaa
     4561 gagaggctga agctgaattc gcagtaccac catcaggtaa aagggcaacc tgtagtaatg
     4621 gaaagacggt aaacaatgat gcgtgctgcg tttggttcga cgtgttagac gacatccaaa
     4681 cgaacttatt ccacggtgga caatgcggag aggacgcaca cgaggcgtta cgtttaacgt
     4741 ttcacgacgc cattgcattc agtccagcgt tgtgggcaca aggacaattt ggaggaggtg
     4801 gtgctgacgg atcaattatc gcacactctg acatcgagct aacctatccg gccaataacg
     4861 gaattgacga gatcgtcgag gcatcgcgtc acatcgctca gaagcacaac gtgtccttcg
     4921 gggactttat tcagtttgcc ggagcagttg gagtcgccaa ctgcaatggt ggaccacagt
     4981 taccattctt cgccggccgt ccaaatccat cccagcccgc tccacccaac cttgtcccgc
     5041 tgccgtcaga ctcggctgat cagattttag cccgtttcgc agacgcggga ttctcagccg
     5101 tagaggttgt gtggttactt gtatctcaca cagtgggttc gcagcacact gtagacccat
     5161 ctatcccagg tgcacctttc gactccaccc cctcggactt tgacgcgcaa ttcttcgtcg
     5221 agacaatgtt gaatggtaca ctggtcccag gaaacggatt acaacaggga gaggtaaact
     5281 caccttaccc gggcgaattt agattacagt ctgacttcct gctagctcgt gaccccagga
     5341 ctgcatgtga gtggcaaaag atgattgcag accaggataa catgcaatcg aagttcgccg
     5401 ctgtgatgtt aaagatgtcg ctattagggt tcgatcagag ttccttaatc gattgttccg
     5461 acgtcatacc tacgccacct ggcacggtac aggacccatt tctacctgcc ggtctgactg
     5521 tcgatgactt gcagcctgct tgcagcgaca gccctttccc aaccgtgcca actgtacctg
     5581 gtccagctac gtccatacca cccgttccca tggactcata agcggccgct cgagcatgca
     5641 tctagagggc cgcatcatgt aattagttat gtcacgctta cattcacgcc ctccccccac
     5701 atccgctcta accgaaaagg aaggagttag acaacctgaa gtctaggtcc ctatttattt
     5761 ttttatagtt atgttagtat taagaacgtt atttatattt caaatttttc ttttttttct
     5821 gtacagacgc gtgtacgcat gtaacattat actgaaaacc ttgcttgaga aggttttggg
     5881 acgctcgaag gctttaattt gcaagctgcg gccctgcatt aatgaatcgg ccaacgcgcg
     5941 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc
     6001 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
     6061 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
     6121 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     6181 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     6241 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     6301 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     6361 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     6421 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     6481 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     6541 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
     6601 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     6661 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     6721 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     6781 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     6841 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
     6901 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
     6961 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
     7021 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
     7081 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
     7141 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
     7201 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
     7261 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
     7321 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
     7381 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
     7441 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
     7501 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
     7561 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
     7621 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
     7681 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
     7741 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatgggt aataactgat
     7801 ataattaaat tgaagctcta atttgtgagt ttagtataca tgcatttact tataatacag
     7861 ttttttagtt ttgctggccg catcttctca aatatgcttc ccagcctgct tttctgtaac
     7921 gttcaccctc taccttagca tcccttccct ttgcaaatag tcctcttcca acaataataa
     7981 tgtcagatcc tgtagagacc acatcatcca cggttctata ctgttgaccc aatgcgtctc
     8041 ccttgtcatc taaacccaca ccgggtgtca taatcaacca atcgtaacct tcatctcttc
     8101 cacccatgtc tctttgagca ataaagccga taacaaaatc tttgtcgctc ttcgcaatgt
     8161 caacagtacc cttagtatat tctccagtag atagggagcc cttgcatgac aattctgcta
     8221 acatcaaaag gcctctaggt tcctttgtta cttcttctgc cgcctgcttc aaaccgctaa
     8281 caatacctgg gcccaccaca ccgtgtgcat tcgtaatgtc tgcccattct gctattctgt
     8341 atacacccgc agagtactgc aatttgactg tattaccaat gtcagcaaat tttctgtctt
     8401 cgaagagtaa aaaattgtac ttggcggata atgcctttag cggcttaact gtgccctcca
     8461 tggaaaaatc agtcaagata tccacatgtg tttttagtaa acaaattttg ggacctaatg
     8521 cttcaactaa ctccagtaat tccttggtgg tacgaacatc caatgaagca cacaagtttg
     8581 tttgcttttc gtgcatgata ttaaatagct tggcagcaac aggactagga tgagtagcag
     8641 cacgttcctt atatgtagct ttcgacatga tttatcttcg tttcctgcag gtttttgttc
     8701 tgtgcagttg ggttaagaat actgggcaat ttcatgtttc ttcaacacta catatgcgta
     8761 tatataccaa tctaagtctg tgctccttcc ttcgttcttc cttctgttcg gagattaccg
     8821 aatcaaaaaa atttcaagga aaccgaaatc aaaaaaaaga ataaaaaaaa aatgatgaat
     8881 tgaaaagcta gcaggtacgt agtatataga gacaaaatag aagaaaccgt tcataatttt
     8941 ctgaccaatg aagaatcatc aacgctatca ctttctgttc acaaagtatg cgcaatccac
     9001 atcggtatag aatataatcg gggatgcctt tatcttgaaa aaatgcaccc gcagcttcgc
     9061 tagtaatcag taaacgcggg aagtggagtc aggctttttt tatggaagag aaaatagaca
     9121 ccaaagtagc cttcttctaa ccttaacgga cctacagtgc aaaaagttat caagagactg
     9181 cattatagag cgcacaaagg agaaaaaaag taatctaaga tgctttgtta gaaaaatagc
     9241 gctctcggga tgcatttttg tagaacaaaa aagaagtata gattctttgt tggtaaaata
     9301 gcgctctcgc gttgcatttc tgttctgtaa aaatgcagct cagattcttt gtttgaaaaa
     9361 ttagcgctct cgcgttgcat ttttgtttta caaaaatgaa gcacagattc ttcgttggta
     9421 aaatagcgct ttcgcgttgc atttctgttc tgtaaaaatg cagctcagat tctttgtttg
     9481 aaaaattagc gctctcgcgt tgcatttttg ttctacaaaa tgaagcacag atgcttcgtt
     9541 aacaaagata tgctattgaa gtgcaagatg gaaacgcaga aaatgaaccg gggatgcgac
     9601 gtgcaagatt acctatgcaa tagatgcaat agtttctcca ggaaccgaaa tacatacatt
     9661 gtcttccgta aagcgctaga ctatatatta ttatacaggt tcaaatatac tatctgtttc
     9721 agggaaaact cccaggttcg gatgttcaaa attcaatgat gggtaacaag tacgatcgta
     9781 aatctgtaaa acagtttgtc ggatattagg ctgtatctcc tcaaagcgta ttcgaatatc
     9841 attgagaagc tgcagcgtca catcggataa taatgatggc agccattgta gaagtgcctt
     9901 ttgcatttct agtctctttc tcgatctagc tagttttact acatcgcgaa gatagaatct
     9961 tagatcacac tgcctttgct gagctggatc aatagagtaa caaaagagtg gtaaggcctc
    10021 gttaaaggac aaggacctga gcggaagtgt atcgtacagt agacggagta tactagtata
    10081 gtctatagtc cgtggaattc taagtgccag ctttataacg tcattctcct tactacagac
    10141 ccgcctgaaa gtagacacat catcatcagt aagctttgac aaaaagcatt gagtagctaa
    10201 ctcttctatg caatctatag ctgttttata aggcattcaa tggacagatt gaggtttttg
    10261 aaacatacta gtgaaattag ccttaatccc ttctcgaagt taatcatgca ttatggtgta
    10321 aaaaatgcaa ctcgcgttgc tctacttttt cccgaatttc caaatacgca gctggggtga
    10381 ttgctcgatt tcgtaacgaa agttttgttt ataaaaaccg cgaaaacctt ctgtaacaga
    10441 tagattttta cagcgctgat atacaatgac atcagctgta atggaaaata actgaaatat
    10501 gaatggcgag agactgcttg cttgtattaa gcaatgtatt atgcagcact tccaacctat
    10561 ggtgtacgat gaaagtaggt gtgtaatcga gacgacaagg gggacttttc cagttcctga
    10621 caattataag aaatacaaaa cgttagcatt tgcatttgtt ggacatgtac tgaatacaga
    10681 cgacacaccg gtaattgaaa aagaactgga ttggcctgat cctgcactag tgtacaatac
    10741 aattgtcgat cgaatcataa atcacccaga attatcacag tttatatcgg ttgcatttat
    10801 tagtcagtta aaggccacca tcggagaggg tttagatatt aatgtaaaag gcacgctaaa
    10861 ccgcagggga aagggtatca gaaggcctaa aggcgtattt tttagataca tggaatctcc
    10921 atttgtcaat acaaaggtca ctgcattctt ctcttatctt cgagattata ataaaattgc
    10981 ctcagaatat cacaataata ctaaattcat tctcacgttt tcatgtcaag catattgggc
    11041 atctggccca aacttctccg ccttgaagaa tgttattagg tgctccataa ttcatgaata
    11101 catttctaag tttgtggaaa gagaacagga taaaggtcat ataggagatc aggagctacc
    11161 gcctgaagag gacccttctc gtgaactaaa caatgtacaa catgaagtca atagtttaac
    11221 ggaacaagat gcggaggcgg atgaaggatt gtggggtgaa atagattcat tatgtgaaaa
    11281 atggcagtct gaagcggaag atcaaactga ggcggagata atagccgaca ggataattgg
    11341 aaatagccag aggatggcga acctcaaaat tcgtcgtaca aagttcaaaa gtgtcttgta
    11401 tcatatacta aaggaactaa ttcaatctca gggaaccgta aaggtttatc gcggtagtag
    11461 tttttcacac gattcgataa agataagctt acattatgaa gagcagcata ttacagccgt
    11521 atgggtctac ttgacagtaa aatttgaaga gcattggaag cctgttgatg tagaggtcga
    11581 gtttagatgc aagttcaagg agcgaaaggt ggatgggtag gttatatagg gatatagcac
    11641 agagatatat agcaaagaga tacttttgag caatgtttgt ggaagcggta ttcgcaatat
    11701 tttagtagct cgttacagtc cggtgcgttt ttggtttttt gaaagtgcgt cttcagagcg
    11761 cttttggttt tcaaaagcgc tct
//
