LOCUS       sequence-37015-e         860 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 860)
  AUTHORS   Thomas H, Jaschkowitz K, Bulman M, Frayling TM, Mitchell SM, Roosen 
            S, Lingott-Frieg A, Tack CJ, Ellard S, Ryffel GU, Hattersley AT
  TITLE     A distant upstream promoter of the HNF-4alpha gene connects the 
            transcription factors involved in maturity-onset diabetes of the 
            young.
  JOURNAL   Hum Mol Genet. 2001 Sep 15. 10(19):2089-97.
  PUBMED    11590126
REFERENCE   2  (bases 1 to 860)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Hum Mol 
            Genet. 2001 Sep 15. 10(19):2089-97."
COMMENT     Sequence Label: HNF4A_P2-257
FEATURES             Location/Qualifiers
     source          1..860
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      47..56
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    390..481
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     complement(422..441)
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     polyA_signal    495..543
                     /note="synthetic polyadenylation signal"
     primer_bind     complement(811..832)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 ctcncgnann atggcgccgg gcctttcttt atgtttttgg cgtcttccat ggtggcttta
       61 ccaacagtac cggaatgcca agcttgggcc aagcccaccc agccggagag ctgggggcat
      121 ggaggagcag gggcccgctc acagcagcag cacagccacc aggagcccgt ccaccaggaa
      181 ggcggtgagt gcggccagct gccaccgccc ggttatctta ttgattcttc taatcaccca
      241 aggtgggtgg atacgttaaa gagtaaccag tcacttaggg aacccgcggc tggggactgt
      301 tgctgtctcc atggcaatgg cgacctggcc agacctgagg tcccgctcga gcccgggcta
      361 gcacgcgtaa gagctcggta cctatcgata gagaaatgtt ctggcacctg cacttgcact
      421 ggggacagcc tattttgcta gtttgttttg tttcgttttg ttttgatgga gagcgtatgt
      481 tagtactatc gattcacaca aaaaaccaac acacagatgt aatgaaaata aagatatttt
      541 attgcggccg ctccaagtac ctcccgtacc ttaatattac ttacttatca tggtagcttg
      601 ggctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg
      661 aatggcgaat ggcaaattgt aagcgttaat attttgttaa aattcgcgtt aaatttttgt
      721 taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa
      781 gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaag
      841 aacgtggact ccaacgtcaa
//
