LOCUS       sequence-37016-e         860 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 860)
  AUTHORS   Thomas H, Jaschkowitz K, Bulman M, Frayling TM, Mitchell SM, Roosen 
            S, Lingott-Frieg A, Tack CJ, Ellard S, Ryffel GU, Hattersley AT
  TITLE     A distant upstream promoter of the HNF-4alpha gene connects the 
            transcription factors involved in maturity-onset diabetes of the 
            young.
  JOURNAL   Hum Mol Genet. 2001 Sep 15. 10(19):2089-97.
  PUBMED    11590126
REFERENCE   2  (bases 1 to 860)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Hum Mol 
            Genet. 2001 Sep 15. 10(19):2089-97."
COMMENT     Sequence Label: HNF4A_P2-226
FEATURES             Location/Qualifiers
     source          1..860
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      35..44
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    348..439
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     complement(380..399)
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     polyA_signal    453..501
                     /note="synthetic polyadenylation signal"
     primer_bind     complement(769..790)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 ggcgccgggc ctttctttat gtttttggcg tcttccatgg tggctttacc aacagtaccg
       61 gaatgccaag cttgggccaa gcccacccag ccggagagct gggggcatgg aggagcaggg
      121 gcccgctcac agcagcagca cagccaccag gagcccgtcc accaggaagg cggtgagtgc
      181 ggccagctgc caccgcccgg ttatcttatt gattcttcta atcacccaag gtgggtggat
      241 acgttaaaga gtaaccagtc acttagggaa cccgcggctg gggactgttg ctgtctccat
      301 ccgctcgagc ccgggctagc acgcgtaaga gctcggtacc tatcgataga gaaatgttct
      361 ggcacctgca cttgcactgg ggacagccta ttttgctagt ttgttttgtt tcgttttgtt
      421 ttgatggaga gcgtatgtta gtactatcga ttcacacaaa aaaccaacac acagatgtaa
      481 tgaaaataaa gatattttat tgcggccgct ccaagtacct cccgtacctt aatattactt
      541 acttatcatg gtagcttggg ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc
      601 caacagttgc gcagcctgaa tggcgaatgg caaattgtaa gcgttaatat tttgttaaaa
      661 ttcgcgttaa atttttgtta aatcagctca ttttttaacc aataggccga aatcggcaaa
      721 atcccttata aatcaaaaga atagaccgag atagggttga gtgttgttcc agtttggaac
      781 aagagtccac tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag
      841 ggcgatggcc cactacgtga
//
