LOCUS       sequence-370166-        5724 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AAV-mediated expression of GCaMP8f under the control of GfaABC1D 
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5724)
  TITLE     GCaMP8/jGCaMP8 AAV plasmids
REFERENCE   2  (bases 1 to 5724)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pZac2.1 gfaABC1D-cyto-GCaMP8f
FEATURES             Location/Qualifiers
     source          1..5724
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          812..944
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     927..946
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        989..1007
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1033..1050
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     1332..1353
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(1459..1480)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1720..1739)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     polyA_signal    2322..2443
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2333..2352)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2387..2406
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     repeat_region   2605..2734
                     /label=AAV2 ITR
     primer_bind     complement(2753..2770)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2753..2769)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2762..2784)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      2911..3366
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2998..3017)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3208..3229
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        3392..3496
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3497..3565,3566..4357)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      4528..5116
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5017..5036
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    5404..5425
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5440..5445,5446..5463,5464..5470)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5478..5494
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5483..5505
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5502..5518
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5502..5518
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     repeat_region   5556..5685
                     /label=AAV2 ITR
ORIGIN
        1 agccatgctc taggaagatc taacatatcc tggtgtggag taggggacgc tgctctgaca
       61 gaggctcggg ggcctgagct ggctctgtga gctggggagg aggcagacag ccaggccttg
      121 tctgcaagca gacctggcag cattgggctg gccgcccccc agggcctcct cttcatgccc
      181 agtgaatgac tcaccttggc acagacacaa tgttcggggt gggcacagtg cctgcttccc
      241 gccgcacccc agcccccctc aaatgccttc cgagaagccc attgagcagg gggcttgcat
      301 tgcaccccag cctgacagcc tggcatcttg ggataaaagc agcacagccc cctaggggct
      361 gcccttgctg tgtggcgcca ccggcggtgg agaacaaggc tctattcagc ctgtgcccag
      421 gaaaggggat caggggatgc ccaggcatgg acagtgggtg gcaggggggg agaggagggc
      481 tgtctgcttc ccagaagtcc aaggacacaa atgggtgagg ggagagctct ccccatagct
      541 gggctgcggc ccaaccccac cccctcaggc tatgccaggg ggtgttgcca ggggcacccg
      601 ggcatcgcca gtctagccca ctccttcata aagccctcgc atcccaggag cgagcagagc
      661 cagagcaggt tggagaggag acgcatcacc tccgctgctc gcaagcttta ttgcggtagt
      721 ttatcacagt taaattgcta acgcagtcag tgcttctgac acaacagtct cgaacttaag
      781 ctgcagaagt tggtcgtgag gcactgggca ggtaagtatc aaggttacaa gacaggttta
      841 aggagaccaa tagaaactgg gcttgtcgag acagagaaga ctcttgcgtt tctgataggc
      901 acctattggt cttactgaca tccactttgc ctttctctcc acaggtgtcc actcccagtt
      961 caattacagc tcttaaggct agagtactta atacgactca ctataggcta gcctcgagaa
     1021 ttcaccatgg gccatcatca ccatcatcac acgcgtcgca agaagacctt caaggaggtg
     1081 gccaccgccg tgaagatcat cgcccgcctg atgggcctga agatcaacgt ctatatcaag
     1141 gccgacaagc agaagaacgg catcaaggcg aacttccaca tccgccacaa catcgaggac
     1201 ggcggcgtgc agctcgccta ccactaccag cagaacaccc ccatcggcga cggccccgtg
     1261 ctgctgcccg acaaccacta cctgagcgtg gagtccaaac tttcgaaaga ccccaacgag
     1321 aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg
     1381 gacgagctgt acaagggcgg taccggaggg agcatggtga gcaagggcga ggagctgttc
     1441 accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc
     1501 gtgtccggcg agggtgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc
     1561 accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgac ctacggcgtg
     1621 cagtgcttca gccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg
     1681 cccgaaggct acatccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc
     1741 cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc
     1801 gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacct gccggaccaa
     1861 ctgactgaag agcagatcgc agaatttaaa gaggctttct ccctatttga caaggacggg
     1921 gatgggacaa taacaaccaa ggagctgggg acggtgatgc ggtctctcgg actgaacccc
     1981 acagaagcag agctgcagga catgatcaat gaagtagatg ccgacggtga cggcacaatc
     2041 gacttccctg agttcctgac aatgatggca agaaaaatga aatacaggga cacggaagaa
     2101 gaaattagag aagcgttcgg tgtgtttgat aaggatggca atggctacat cagtgcagca
     2161 gagcttcgcc acgtgatgac aaaccttgga gagaagttaa cagatgaaga ggttgatgaa
     2221 atgatcaggg aagcagacat cgatggggat ggtcaggtaa actacgaaga gtttgtacaa
     2281 atgatgacag cgaagtaagc ggccgcttcg agcagacatg ataagataca ttgatgagtt
     2341 tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc
     2401 tattgcttta tttgtaacca ttataagctg caataaacaa gttaacaaca acaattgcat
     2461 tcattttatg tttcaggttc agggggagat gtgggaggtt ttttaaagca agtaaaacct
     2521 ctacaaatgt ggtaaaatcg ataaggatct tcctagagca tggctacgta gataagtagc
     2581 atggcgggtt aatcattaac tacaaggaac ccctagtgat ggagttggcc actccctctc
     2641 tgcgcgctcg ctcgctcact gaggccgggc gaccaaaggt cgcccgacgc ccgggctttg
     2701 cccgggcggc ctcagtgagc gagcgagcgc gcagccttaa ttaacctaat tcactggccg
     2761 tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag
     2821 cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc
     2881 aacagttgcg cagcctgaat ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg
     2941 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc
     3001 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
     3061 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
     3121 ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt
     3181 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca
     3241 accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt
     3301 taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgctta
     3361 caatttaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
     3421 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
     3481 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
     3541 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
     3601 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
     3661 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
     3721 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
     3781 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
     3841 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
     3901 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
     3961 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
     4021 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga
     4081 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
     4141 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
     4201 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
     4261 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
     4321 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
     4381 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
     4441 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     4501 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     4561 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     4621 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
     4681 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     4741 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     4801 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     4861 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     4921 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     4981 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     5041 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     5101 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     5161 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
     5221 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
     5281 gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat
     5341 tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc
     5401 aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc
     5461 tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacca
     5521 tgattacgcc agatttaatt aaggccttaa ttaggctgcg cgctcgctcg ctcactgagg
     5581 ccgcccgggc aaagcccggg cgtcgggcga cctttggtcg cccggcctca gtgagcgagc
     5641 gagcgcgcag agagggagtg gccaactcca tcactagggg ttccttgtag ttaatgatta
     5701 acccgccatg ctacttatct acgt
//
