LOCUS       sequence-37018-e         830 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 830)
  AUTHORS   Thomas H, Jaschkowitz K, Bulman M, Frayling TM, Mitchell SM, Roosen 
            S, Lingott-Frieg A, Tack CJ, Ellard S, Ryffel GU, Hattersley AT
  TITLE     A distant upstream promoter of the HNF-4alpha gene connects the 
            transcription factors involved in maturity-onset diabetes of the 
            young.
  JOURNAL   Hum Mol Genet. 2001 Sep 15. 10(19):2089-97.
  PUBMED    11590126
REFERENCE   2  (bases 1 to 830)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Hum Mol 
            Genet. 2001 Sep 15. 10(19):2089-97."
COMMENT     Sequence Label: HNF4A_P2-164
FEATURES             Location/Qualifiers
     source          1..830
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      49..58
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    300..391
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     complement(332..351)
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     polyA_signal    405..453
                     /note="synthetic polyadenylation signal"
     primer_bind     complement(721..742)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 gcttcnggat gaatggcgcc gggcctttct ttatgttttt ggcgtcttcc atggtggctt
       61 taccaacagt accggaatgc caagcttggg ccaagcccac ccagccggag agctgggggc
      121 atggaggagc aggggcccgc tcacagcagc agcacagcca ccaggagccc gtccaccagg
      181 aaggcggtga gtgcggccag ctgccaccgc ccggttatct tattgattct tctaatcacc
      241 caaggtgggt ggccgctcga gcccgggcta gcacgcgtaa gagctcggta cctatcgata
      301 gagaaatgtt ctggcacctg cacttgcact ggggacagcc tattttgcta gtttgttttg
      361 tttcgttttg ttttgatgga gagcgtatgt tagtactatc gattcacaca aaaaaccaac
      421 acacagatgt aatgaaaata aagatatttt attgcggccg ctccaagtac ctcccgtacc
      481 ttaatattac ttacttatca tggtagcttg ggctggcgta atagcgaaga ggcccgcacc
      541 gatcgccctt cccaacagtt gcgcagcctg aatggcgaat ggcaaattgt aagcgttaat
      601 attttgttaa aattcgcgtt aaatttttgt taaatcagct cattttttaa ccaataggcc
      661 gaaatcggca aaatccctta taaatcaaaa gaatagaccg agatagggtt gagtgttgtt
      721 ccagtttgga acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa
      781 accgtctatc agggcgatgg cccactacgt gaaccatcac cctaatcaag
//
