LOCUS       sequence-370181-        9805 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression TCR alpha(1G4).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9805)
  AUTHORS   Susac L, Vuong MT, Thomas C, von Bulow S, O'Brien-Ball C, Santos AM,
            Fernandes RA, Hummer G, Tampe R, Davis SJ
  TITLE     Structure of a fully assembled tumor-specific T cell receptor 
            ligated by pMHC.
  JOURNAL   Cell. 2022 Aug 18;185(17):3201-3213.e19. doi: 
            10.1016/j.cell.2022.07.010.
  PUBMED    35985289
REFERENCE   2  (bases 1 to 9805)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.07"; journalName: "Cell"; date: "2022-08-18- 
            18"; volume: "185"; issue: "17"; pages: "3201-3213.e19"
COMMENT     Sequence Label: pHRSin_1G4 TCR-alpha
FEATURES             Location/Qualifiers
     source          1..9805
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          1067..1132
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             1262..1282
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    1707..1841
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1744..1763)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1798..1817
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             2009..2642
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    2689..2814
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3311..3544
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3729..3773
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4058..4175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        4328..4735
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     regulatory      4756..4765
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    5673..6261
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5726..5746)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6144..6155)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6264..6280)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6265..6281)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             6375..6608
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(6633..6651)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(6634..6651)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(6941..6960)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(7174..7193)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7293..7315
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7353..7371)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7439..7543
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7544..7612,7613..8404)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7762..7781)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8575..9163
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9064..9083
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9317..9334
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(9404..9424)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9409..9738
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9589..9724
                     /label=SV40 ori
                     /note="SV40 origin of replication"
ORIGIN
        1 ggcagtgcgt aaaaagacgc ggactcatgt gaaatactgg tttttagtgc gccagatctc
       61 tataatctcg cgcaacctat tttcccctcg aacacttttt aagccgtaga taaacaggct
      121 gggacacttc acatgagcga aaaatacatc gtcacctggg acatgttgca gatccatgca
      181 cgtaaactcg caagccgact gatgccttct gaacaatgga aaggcattat tgccgtaagc
      241 cgtggcggtc tgtaccgggt gcgttactgg cgcgtgaact gggtattcgt catgtcgata
      301 ccgtttgtat ttccagctac gatcacgaca accagcgcga gcttaaagtg ctgaaacgcg
      361 cagaaggcga tggcgaaggc ttcatcgtta ttgatgacct ggtggatacc ggtggtactg
      421 cggttgcgat tcgtgaaatg tatccaaaag cgcactttgt caccatcttc gcaaaaccgg
      481 ctggtcgtcc gctggttgat gactatgttg ttgatatccc gcaagatacc tggattgaac
      541 agccgtggga tatgggcgtc gtattcgtcc cgccaatctc cggtcgctaa tcttttcaac
      601 gcctggcact gccgggcgtt gttcttttta acttcaggcg ggttacaata gtttccagta
      661 agtattctgg aggctgcatc catgacacag gcaaacctga gcgaaaccct gttcaaaccc
      721 cgctttaaac atcctgaaac ctcgacgcta gtccgccgct ttaatcacgg cgcacaaccg
      781 cctgtgcagt cggcccttga tggtaaaacc atccctcact ggtatcgcat gattaaccgt
      841 ctgatgtgga tctggcgcgg cattgaccca cgcgaaatcc tcgacgtcca ggcacgtatt
      901 gtgatgagcg atgccgaacg taccgacgat gatttatacg atacggtgat tggctaccgt
      961 ggcggcaact ggatttatga gtgggccccg gatctttgtg aaggaacctt acttctgtgg
     1021 tgtgacataa ttggacaaac tacctacaga gatttaaagc tctaaggtaa atataaaatt
     1081 tttaagtgta taatgtgtta aactactgat tctaattgtt tgtgtatttt agattccaac
     1141 ctatggaact gatgaatggg agcagtggtg gaatgccttt aatgaggaaa acctgttttg
     1201 ctcagaagaa atgccatcta gtgatgatga ggctactgct gactctcaac attctactcc
     1261 tccaaaaaag aagagaaagg tagaagaccc caaggacttt ccttcagaat tgctaagttt
     1321 tttgagtcat gctgtgttta gtaatagaac tcttgcttgc tttgctattt acaccacaaa
     1381 ggaaaaagct gcactgctat acaagaaaat tatggaaaaa tattctgtaa cctttataag
     1441 taggcataac agttataatc ataacatact gttttttctt actccacaca ggcatagagt
     1501 gtctgctatt aataactatg ctcaaaaatt gtgtaccttt agctttttaa tttgtaaagg
     1561 ggttaataag gaatatttga tgtatagtgc cttgactaga gatcataatc agccatacca
     1621 catttgtaga ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac
     1681 ataaaatgaa tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat
     1741 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
     1801 gtttgtccaa actcatcaat gtatcttatc atgtctggat caactggata actcaagcta
     1861 accaaaatca tcccaaactt cccaccccat accctattac cactgccaat tacctgtggt
     1921 ttcatttact ctaaacctgt gattcctctg aattattttc attttaaaga aattgtattt
     1981 gttaaatatg tactacaaac ttagtagttg gaagggctaa ttcactccca aagaagacaa
     2041 gatatccttg atctgtggat ctaccacaca caaggctact tccctgatta gcagaactac
     2101 acaccagggc caggggtcag atatccactg acctttggat ggtgctacaa gctagtacca
     2161 gttgagccag ataaggtaga agaggccaat aaaggagaga acaccagctt gttacaccct
     2221 gtgagcctgc atgggatgga tgacccggag agagaagtgt tagagtggag gtttgacagc
     2281 cgcctagcat ttcatcacgt ggcccgagag ctgcatccgg agtacttcaa gaactgctga
     2341 tatcgagctt gctacaaggg actttccgct ggggactttc cagggaggcg tggcctgggc
     2401 gggactgggg agtggcgagc cctcagatcc tgcatataag cagctgcttt ttgcctgtac
     2461 tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc
     2521 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt
     2581 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag
     2641 cagtggcgcc cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc
     2701 aggactcggc ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg
     2761 ccaaaaattt tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt
     2821 aagcggggga gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa
     2881 aaatataaat taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat
     2941 cctggcctgt tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc
     3001 cttcagacag gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt
     3061 gtgcatcaaa ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag
     3121 caaaacaaaa gtaagaccac cgcacagcaa gcggccggcc gctgatcttc agacctggag
     3181 gaggagatat gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg
     3241 aaccattagg agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag
     3301 cagtgggaat aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg
     3361 cagcgtcaat gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc
     3421 agaacaattt gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg
     3481 gcatcaagca gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc
     3541 tcctggggat ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg
     3601 ctagttggag taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg
     3661 acagagaaat taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc
     3721 agcaagaaaa gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt
     3781 ggtttaacat aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct
     3841 tggtaggttt aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat
     3901 attcaccatt atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag
     3961 gaatagaaga agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat
     4021 ctcgacggta tcgccaaatg gcagtattca tccacaattt taaaagaaaa ggggggattg
     4081 gggggtacag tgcaggggaa agaatagtag acataatagc aacagacata caaactaaag
     4141 aattacaaaa acaaattaca aaaattcaaa attttcgggt ttattacagg gacagcagag
     4201 atccagtttg gatcgataag cttgatatcg aattcctgca gccccgataa aataaaagat
     4261 tttatttagt ctccagaaaa aggggggaat gaaagacccc acctgtaggt ttggcaagct
     4321 agctgcagta acgccatttt gcaaggcatg gaaaaatacc aaaccaagaa tagagaagtt
     4381 cagatcaagg gcgggtacat gaaaatagct aacgttgggc caaacaggat atctgcggtg
     4441 agcagtttcg gccccggccc ggggccaaga acagatggtc accgcagttt cggccccggc
     4501 ccgaggccaa gaacagatgg tccccagata tggcccaacc ctcagcagtt tcttaagacc
     4561 catcagatgt ttccaggctc ccccaaggac ctgaaatgac cctgcgcctt atttgaatta
     4621 accaatcagc ctgcttctcg cttctgttcg cgcgcttctg cttcccgagc tctataaaag
     4681 agctcacaac ccctcactcg gcgcgccagt cctccgacag actgagtcgc ccggggggga
     4741 tccgaattca cgcgtgccac catggggata cttcccagcc ctgggatgcc tgcgctgctc
     4801 tccctcgtga gccttctctc cgtgctgctg atgggttgcg tagctaaaca ggaggtgacg
     4861 cagattcctg cagctctgag tgtcccagaa ggagaaaact tggttctcaa ctgcagtttc
     4921 actgatagcg ctatttacaa cctccagtgg tttaggcagg accctgggaa aggtctcaca
     4981 tctctgttgc ttattcagtc aagtcagaga gagcaaacaa gtggaagact taatgcctcg
     5041 ctggataaat catcaggacg tagtacttta tacattgcag cttctcagcc tggtgactca
     5101 gccacctacc tctgtgctgt gaggcccaca tcaggaggaa gctacatacc tacatttgga
     5161 agaggaacca gccttattgt tcatccgtat atccagaacc ctgaccctgc cgtgtaccag
     5221 ctgagagact ctaaatccag tgacaagtct gtctgcctat tcaccgattt tgattctcaa
     5281 acaaatgtgt cacaaagtaa ggattctgat gtgtatatca cagacaaaac tgtgctagac
     5341 atgaggtcta tggacttcaa gagcaacagt gctgtggcct ggagcaacaa atctgacttt
     5401 gcatgtgcaa acgccttcaa caacagcatt attccagaag acaccttctt ccccagccca
     5461 gaaagttcct gtgatgtcaa gctggtcgag aaaagctttg aaacagatac gaacctaaac
     5521 tttcaaaacc tgtcagtgat tgggttccga atcctcctcc tgaaagtggc cgggtttaat
     5581 ctgctcatga cgctgcggct gtggtccagc tgagcggccg cgactctaga gtcgacctgc
     5641 aggcatgcaa gcttgatatc aagcttatcg ataatcaacc tctggattac aaaatttgtg
     5701 aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt
     5761 taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata
     5821 aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg
     5881 tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc
     5941 tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct
     6001 gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt
     6061 cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg
     6121 ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc
     6181 tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct
     6241 ccctttgggc cgcctccccg catcgatacc gtcgacctcg atcgagggaa ttaattcgag
     6301 ctcggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa
     6361 agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagatc tgctttttgc
     6421 ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg
     6481 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg
     6541 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat
     6601 ctctagcagc atctagaatt aattccgtgt attctatagt gtcacctaaa tcgtatgtgt
     6661 atgatacata aggttatgta ttaattgtag ccgcgttcta acgacaatat gtacaagcct
     6721 aattgtgtag catctggctt actgaagcag accctatcat ctctctcgta aactgccgtc
     6781 agagtcggtt tggttggacg aaccttctga gtttctggta acgccgtccc gcacccggaa
     6841 atggtcagcg aaccaatcag cagggtcatc gctagccaga tcctctacgc cggacgcatc
     6901 gtggccggca tcaccggcgc cacaggtgcg gttgctggcg cctatatcgc cgacatcacc
     6961 gatggggaag atcgggctcg ccacttcggg ctcatgagcg cttgtttcgg cgtgggtatg
     7021 gtggcaggcc ccgtggccgg gggactgttg ggcgccatct ccttgcatgc accattcctt
     7081 gcggcggcgg tgctcaacgg cctcaaccta ctactgggct gcttcctaat gcaggagtcg
     7141 cataagggag agcgtcgaat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt
     7201 aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc
     7261 ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc
     7321 accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat ttttataggt
     7381 taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg
     7441 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
     7501 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
     7561 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
     7621 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
     7681 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat
     7741 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca
     7801 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
     7861 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
     7921 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct
     7981 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
     8041 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac
     8101 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
     8161 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
     8221 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc
     8281 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
     8341 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
     8401 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
     8461 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
     8521 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     8581 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     8641 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     8701 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa
     8761 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     8821 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     8881 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     8941 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     9001 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     9061 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     9121 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     9181 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
     9241 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag
     9301 ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa
     9361 accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tgtggaatgt gtgtcagtta
     9421 gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
     9481 tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
     9541 atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta
     9601 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
     9661 gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga
     9721 ggcctaggct tttgcaaaaa gcttggacac aagacaggct tgcgagatat gtttgagaat
     9781 accactttat cccgcgtcag ggaga
//
