LOCUS       sequence-370183-       10516 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of hTCR beta(1G4), and SNAP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10516)
  AUTHORS   Susac L, Vuong MT, Thomas C, von Bulow S, O'Brien-Ball C, Santos AM,
            Fernandes RA, Hummer G, Tampe R, Davis SJ
  TITLE     Structure of a fully assembled tumor-specific T cell receptor 
            ligated by pMHC.
  JOURNAL   Cell. 2022 Aug 18;185(17):3201-3213.e19. doi: 
            10.1016/j.cell.2022.07.010.
  PUBMED    35985289
REFERENCE   2  (bases 1 to 10516)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.07"; journalName: "Cell"; date: "2022-08-18- 
            18"; volume: "185"; issue: "17"; pages: "3201-3213.e19"
COMMENT     Sequence Label: pHRSin_1G4 hTCR-beta-SNAP
FEATURES             Location/Qualifiers
     source          1..10516
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(45..61)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(46..62)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             152..385
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(410..428)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(411..428)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(718..737)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(951..970)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1070..1092
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(1130..1148)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1216..1320
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1321..1389,1390..2181)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1539..1558)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2352..2940
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2841..2860
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3094..3111
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(3181..3201)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3186..3515
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3366..3501
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          4649..4714
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             4844..4864
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    5289..5423
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5326..5345)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5380..5399
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             5591..6224
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    6271..6396
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    6893..7126
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7311..7355
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    7640..7757
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        7910..8317
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     regulatory      8351..8360
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             9335..9880
                     /codon_start=1
                     /product="human O6-alkylguanine-DNA-alkyltransferase 
                     variant that forms covalent bonds with benzylguanine 
                     derivatives"
                     /label=SNAP-tag(R)
                     /note="fast-reacting variant, optimized for mammalian 
                     expression"
                     /translation="MDKDCEMKRTTLDSPLGKLELSGCEQGLHRIIFLGKGTSAADAVE
                     VPAPAAVLGGPEPLMQATAWLNAYFHQPEAIEEFPVPALHHPVFQQESFTRQVLWKLLK
                     VVKFGEVISYSHLAALAGNPAATAAVKTALSGNPVPILIPCHRVVQGDLDVGGYEGGLA
                     VKEWLLAHEGHRLGKPGLG"
     misc_feature    join(9970..10516,1..42)
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(10023..10043)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(10441..10452)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 gccctcagac gagtcggatc tccctttggg ccgcctcccc gcatcgatac cgtcgacctc
       61 gagggaatta attcgagctc ggtaccttta agaccaatga cttacaaggc agctgtagat
      121 cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaga
      181 caagatctgc tttttgcttg tactgggtct ctctggttag accagatctg agcctgggag
      241 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
      301 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
      361 tagtcagtgt ggaaaatctc tagcagcatc tagaattaat tccgtgtatt ctatagtgtc
      421 acctaaatcg tatgtgtatg atacataagg ttatgtatta attgtagccg cgttctaacg
      481 acaatatgta caagcctaat tgtgtagcat ctggcttact gaagcagacc ctatcatctc
      541 tctcgtaaac tgccgtcaga gtcggtttgg ttggacgaac cttctgagtt tctggtaacg
      601 ccgtcccgca cccggaaatg gtcagcgaac caatcagcag ggtcatcgct agccagatcc
      661 tctacgccgg acgcatcgtg gccggcatca ccggcgccac aggtgcggtt gctggcgcct
      721 atatcgccga catcaccgat ggggaagatc gggctcgcca cttcgggctc atgagcgctt
      781 gtttcggcgt gggtatggtg gcaggccccg tggccggggg actgttgggc gccatctcct
      841 tgcatgcacc attccttgcg gcggcggtgc tcaacggcct caacctacta ctgggctgct
      901 tcctaatgca ggagtcgcat aagggagagc gtcgaatggt gcactctcag tacaatctgc
      961 tctgatgccg catagttaag ccagccccga cacccgccaa cacccgctga cgcgccctga
     1021 cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc
     1081 atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata
     1141 cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact
     1201 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
     1261 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
     1321 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
     1381 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
     1441 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
     1501 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
     1561 cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
     1621 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
     1681 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
     1741 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
     1801 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
     1861 cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
     1921 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
     1981 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct
     2041 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
     2101 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
     2161 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
     2221 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
     2281 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     2341 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     2401 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     2461 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
     2521 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     2581 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     2641 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     2701 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     2761 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     2821 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     2881 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     2941 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     3001 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
     3061 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
     3121 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgt
     3181 ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
     3241 aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag
     3301 gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc
     3361 cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa
     3421 ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt
     3481 gaggaggctt ttttggaggc ctaggctttt gcaaaaagct tggacacaag acaggcttgc
     3541 gagatatgtt tgagaatacc actttatccc gcgtcaggga gaggcagtgc gtaaaaagac
     3601 gcggactcat gtgaaatact ggtttttagt gcgccagatc tctataatct cgcgcaacct
     3661 attttcccct cgaacacttt ttaagccgta gataaacagg ctgggacact tcacatgagc
     3721 gaaaaataca tcgtcacctg ggacatgttg cagatccatg cacgtaaact cgcaagccga
     3781 ctgatgcctt ctgaacaatg gaaaggcatt attgccgtaa gccgtggcgg tctgtaccgg
     3841 gtgcgttact ggcgcgtgaa ctgggtattc gtcatgtcga taccgtttgt atttccagct
     3901 acgatcacga caaccagcgc gagcttaaag tgctgaaacg cgcagaaggc gatggcgaag
     3961 gcttcatcgt tattgatgac ctggtggata ccggtggtac tgcggttgcg attcgtgaaa
     4021 tgtatccaaa agcgcacttt gtcaccatct tcgcaaaacc ggctggtcgt ccgctggttg
     4081 atgactatgt tgttgatatc ccgcaagata cctggattga acagccgtgg gatatgggcg
     4141 tcgtattcgt cccgccaatc tccggtcgct aatcttttca acgcctggca ctgccgggcg
     4201 ttgttctttt taacttcagg cgggttacaa tagtttccag taagtattct ggaggctgca
     4261 tccatgacac aggcaaacct gagcgaaacc ctgttcaaac cccgctttaa acatcctgaa
     4321 acctcgacgc tagtccgccg ctttaatcac ggcgcacaac cgcctgtgca gtcggccctt
     4381 gatggtaaaa ccatccctca ctggtatcgc atgattaacc gtctgatgtg gatctggcgc
     4441 ggcattgacc cacgcgaaat cctcgacgtc caggcacgta ttgtgatgag cgatgccgaa
     4501 cgtaccgacg atgatttata cgatacggtg attggctacc gtggcggcaa ctggatttat
     4561 gagtgggccc cggatctttg tgaaggaacc ttacttctgt ggtgtgacat aattggacaa
     4621 actacctaca gagatttaaa gctctaaggt aaatataaaa tttttaagtg tataatgtgt
     4681 taaactactg attctaattg tttgtgtatt ttagattcca acctatggaa ctgatgaatg
     4741 ggagcagtgg tggaatgcct ttaatgagga aaacctgttt tgctcagaag aaatgccatc
     4801 tagtgatgat gaggctactg ctgactctca acattctact cctccaaaaa agaagagaaa
     4861 ggtagaagac cccaaggact ttccttcaga attgctaagt tttttgagtc atgctgtgtt
     4921 tagtaataga actcttgctt gctttgctat ttacaccaca aaggaaaaag ctgcactgct
     4981 atacaagaaa attatggaaa aatattctgt aacctttata agtaggcata acagttataa
     5041 tcataacata ctgttttttc ttactccaca caggcataga gtgtctgcta ttaataacta
     5101 tgctcaaaaa ttgtgtacct ttagcttttt aatttgtaaa ggggttaata aggaatattt
     5161 gatgtatagt gccttgacta gagatcataa tcagccatac cacatttgta gaggttttac
     5221 ttgctttaaa aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg
     5281 ttgttgttaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
     5341 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
     5401 atgtatctta tcatgtctgg atcaactgga taactcaagc taaccaaaat catcccaaac
     5461 ttcccacccc ataccctatt accactgcca attacctgtg gtttcattta ctctaaacct
     5521 gtgattcctc tgaattattt tcattttaaa gaaattgtat ttgttaaata tgtactacaa
     5581 acttagtagt tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg
     5641 atctaccaca cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc
     5701 agatatccac tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta
     5761 gaagaggcca ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg
     5821 gatgacccgg agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac
     5881 gtggcccgag agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag
     5941 ggactttccg ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga
     6001 gccctcagat cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga
     6061 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata
     6121 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta
     6181 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg
     6241 gacttgaaag cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa
     6301 gcgcgcacgg caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc
     6361 ggaggctaga aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga
     6421 tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat
     6481 atagtatggg caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca
     6541 tcagaaggct gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa
     6601 gaacttagat cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag
     6661 ataaaagaca ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc
     6721 accgcacagc aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca
     6781 attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac
     6841 ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt
     6901 tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga
     6961 cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg
     7021 ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg
     7081 caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt
     7141 gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat
     7201 ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt
     7261 acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac
     7321 aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt
     7381 ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag
     7441 tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc
     7501 agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg
     7561 gagagagaga cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccaaa
     7621 tggcagtatt catccacaat tttaaaagaa aaggggggat tggggggtac agtgcagggg
     7681 aaagaatagt agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta
     7741 caaaaattca aaattttcgg gtttattaca gggacagcag agatccagtt tggatcgata
     7801 agcttgatat cgaattcctg cagccccgat aaaataaaag attttattta gtctccagaa
     7861 aaagggggga atgaaagacc ccacctgtag gtttggcaag ctagctgcag taacgccatt
     7921 ttgcaaggca tggaaaaata ccaaaccaag aatagagaag ttcagatcaa gggcgggtac
     7981 atgaaaatag ctaacgttgg gccaaacagg atatctgcgg tgagcagttt cggccccggc
     8041 ccggggccaa gaacagatgg tcaccgcagt ttcggccccg gcccgaggcc aagaacagat
     8101 ggtccccaga tatggcccaa ccctcagcag tttcttaaga cccatcagat gtttccaggc
     8161 tcccccaagg acctgaaatg accctgcgcc ttatttgaat taaccaatca gcctgcttct
     8221 cgcttctgtt cgcgcgcttc tgcttcccga gctctataaa agagctcaca acccctcact
     8281 cggcgcgcca gtcctccgac agactgagtc gcccgggggg gatctggagc tctcgagaat
     8341 tctcacgcgt gccaccatgg ggatacttcc cagccctggg atgcctgcgc tgctctccct
     8401 cgtgagcctt ctctccgtgc tgctgatggg ttgcgtagct gctggtgtca ctcagacccc
     8461 aaaattccag gtcctgaaga caggacagag catgacactg cagtgtgccc aggatatgaa
     8521 ccatgaatac atgtcctggt atcgacaaga cccaggcatg gggctgaggc tgattcatta
     8581 ctcagttggt gctggtatca ctgaccaagg agaagtcccc aatggctaca atgtctccag
     8641 atcaaccaca gaggatttcc cgctcaggct gctgtcggct gctccctccc agacatctgt
     8701 gtacttctgt gccagcagtt acgtcgggaa caccggggag ctgttttttg gagaaggctc
     8761 taggctgacc gtactggagg acctgaaaaa cgtgttccca cccgaggtcg ctgtgtttga
     8821 gccatcagaa gcagagatct cccacaccca aaaggccaca ctggtgtgcc tggccacagg
     8881 cttctacccc gaccacgtgg agctgagctg gtgggtgaat gggaaggagg tgcacagtgg
     8941 ggtcagcaca gacccgcagc ccctcaagga gcagcccgcc ctcaatgact ccagatactg
     9001 cctgagcagc cgcctgaggg tctcggccac cttctggcag aacccccgca accacttccg
     9061 ctgtcaagtc cagttctacg ggctctcgga gaatgacgag tggacccagg atagggccaa
     9121 acctgtcacc cagatcgtca gcgccgaggc ctggggtaga gcagactgtg gcttcacctc
     9181 cgagtcttac cagcaagggg tcctgtctgc caccatcctc tatgagatct tgctagggaa
     9241 ggccaccttg tatgccgtgc tggtcagtgc cctcgtgctg atggccatgg tcaagagaaa
     9301 ggattccaga ggcggaagcg gaagcggaag cggaatggac aaagactgcg aaatgaagcg
     9361 caccaccctg gatagccctc tgggcaagct ggaactgtct gggtgcgaac agggcctgca
     9421 ccgtatcatc ttcctgggca aaggaacatc tgccgccgac gccgtggaag tgcctgcccc
     9481 agccgccgtg ctgggcggac cagagccact gatgcaggcc accgcctggc tcaacgccta
     9541 ctttcaccag cctgaggcca tcgaggagtt ccctgtgcca gccctgcacc acccagtgtt
     9601 ccagcaggag agctttaccc gccaggtgct gtggaaactg ctgaaagtgg tgaagttcgg
     9661 agaggtcatc agctacagcc acctggccgc cctggccggc aatcccgccg ccaccgccgc
     9721 cgtgaaaacc gccctgagcg gaaatcccgt gcccattctg atcccctgcc accgggtggt
     9781 gcagggcgac ctggacgtgg ggggctacga gggcgggctc gccgtgaaag agtggctgct
     9841 ggcccacgag ggccacagac tgggcaagcc tgggctgggt taggtcaaga gaaaggattt
     9901 cggatcctag gcggccgcga ctctagagtc gacctgcagg catgcaagct tgatatcaag
     9961 cttatcgata atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac
    10021 tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt
    10081 gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat
    10141 gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca
    10201 acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc
    10261 cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg
    10321 gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct
    10381 tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct
    10441 tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt
    10501 ccgcgtcttc gccttc
//
