LOCUS       sequence-370186-        9361 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses CapChR1 in Drosophila.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9361)
  AUTHORS   Fernandez Lahore RG, Pampaloni NP, Peter E, Heim MM, Tillert L, 
            Vierock J, Oppermann J, Walther J, Schmitz D, Owald D, Plested AJR, 
            Rost BR, Hegemann P
  TITLE     Calcium-permeable channelrhodopsins for the photocontrol of calcium 
            signalling.
  JOURNAL   Nat Commun. 2022 Dec 21;13(1):7844. doi: 10.1038/s41467-022-35373-4.
  PUBMED    36543773
REFERENCE   2  (bases 1 to 9361)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-12-21"; volume: "13(1):7844. doi"; pages: " 
            10.1038/s41467-022-35373-4"
COMMENT     Sequence Label: CapChR1_pUAST-attb
FEATURES             Location/Qualifiers
     source          1..9361
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          79..144
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             274..294
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    566..700
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(603..622)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     657..676
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    729..798
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     promoter        complement(1017..1035)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(1056..1072)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1056..1072)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1069..1091)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1080..1096
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1104..1110,1111..1128,1129..1134))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1149..1170
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1287..1304)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1458..2046)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1538..1557)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2217..3008,3009..3077))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2840..2859
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3078..3182)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(3208..3663)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3345..3366)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3557..3576
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3790..3812
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3804..3821
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3805..3821
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3828..3847
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3828..3846
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3880..3898)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(4350..4421,4823..5096,5171..5825,5887..6202,6406..6537,
                     6608..7222)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     protein_bind    7997..8030
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    8063..8157
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     promoter        8176..8414
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     8361..8382
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     regulatory      8429..8438
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             8435..9361
                     /codon_start=1
                     /product="H134R mutant of an N-terminal fragment of 
                     channelrhodopsin-2 from the alga Chlamydomonas reinhardtii 
                     (Nagel et al., 2005)"
                     /label=ChR2(H134R)
                     /note="blue light-activated cation channel"
                     /translation="MDYGGALSAVGRELLFVTNPVVVNGSVLVPEDQCYCAGWIESRGT
                     NGAQTASNVLQWLAAGFDILLLMFYAYQTWKSTCGWEEIYVCAIEMVKVILEFFFEFKN
                     PSMLYLATGHRVQWLRYAEWLLTCPVICIHLSNLTGLSNDYSRRTMGLLVSDIGCIVWG
                     ATSAMATGYVKVIFFCLGLCYGANTFFHAAKAYIEGYHTVPKGRCRQVVTGMAWLFFVS
                     WGMFPILFILGPEGFGVLSVYGSTVGHTIIDLMSKECWGLLGHYLRVLIHEHILIHGDI
                     RKTTKLNIGGTEIEVETLVEDEAEAGAV"
ORIGIN
        1 aggatctttg tgaaggaacc ttacttctgt ggtgtgacat aattggacaa actacctaca
       61 gagatttaaa gctctaaggt aaatataaaa tttttaagtg tataatgtgt taaactactg
      121 attctaattg tttgtgtatt ttagattcca acctatggaa ctgatgaatg ggagcagtgg
      181 tggaatgcct ttaatgagga aaacctgttt tgctcagaag aaatgccatc tagtgatgat
      241 gaggctactg ctgactctca acattctact cctccaaaaa agaagagaaa ggtagaagac
      301 cccaaggact ttccttcaga attgctaagt tttttgagtc atgctgtgtt tagtaataga
      361 actcttgctt gctttgctat ttacaccaca aaggaaaaag ctgcactgct atacaagaaa
      421 attatggaaa aatatttgat gtatagtgcc ttgactagag atcataatca gccataccac
      481 atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca
      541 taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata
      601 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
      661 tttgtccaaa ctcatcaatg tatcttatca tgtctggatc cactagtgtc gacgatgtag
      721 gtcacggtct cgaagccgcg gtgcgggtgc cagggcgtgc ccttgggctc cccgggcgcg
      781 tactccacct cacccatctg gtccatcatg atgaacgggt cgaggtggcg gtagttgatc
      841 ccggcgaacg cgcggcgcac cgggaagccc tcgccctcga aaccgctggg cgcggtggtc
      901 acggtgagca cgggacgtgc gacggcgtcg gcgggtgcgg atacgcgggg cagcgtcagc
      961 gggttctcga cggtcacggc gggcatgtcg acactagttc tagccagctt ttgttccctt
     1021 tagtgagggt taatttcgag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat
     1081 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg
     1141 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag
     1201 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
     1261 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     1321 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     1381 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     1441 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     1501 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     1561 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     1621 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     1681 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     1741 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     1801 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     1861 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
     1921 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     1981 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     2041 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     2101 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     2161 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
     2221 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     2281 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     2341 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
     2401 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     2461 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
     2521 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
     2581 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
     2641 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
     2701 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
     2761 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
     2821 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
     2881 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
     2941 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
     3001 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
     3061 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
     3121 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     3181 cgcacatttc cccgaaaagt gccacctaaa ttgtaagcgt taatattttg ttaaaattcg
     3241 cgttaaattt ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc
     3301 cttataaatc aaaagaatag accgagatag ggttgagtgt tgttccagtt tggaacaaga
     3361 gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg
     3421 atggcccact acgtgaacca tcaccctaat caagtttttt ggggtcgagg tgccgtaaag
     3481 cactaaatcg gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga
     3541 acgtggcgag aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg
     3601 tagcggtcac gctgcgcgta accaccacac ccgccgcgct taatgcgccg ctacagggcg
     3661 cgtcccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt
     3721 cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc
     3781 cagggttttc ccagtcacga cgttgtaaaa cgacggccag tgaattgtaa tacgactcac
     3841 tatagggcga attgggtacg taccgggccc ctagtatgta tgtaagttaa taaaacccat
     3901 ttttgcggaa agtagataaa aaaaacattt ttttttttta ctgcactgga tatcattgaa
     3961 cttatctgat cagttttaaa tttacttcga tccaagggta tttgatgtac caggttcttt
     4021 cgattacctc tcactcaaaa tgacattcca ctcaaagtca gcgctgtttg cctccttctc
     4081 tgtccacaga aatatcgccg tctctttcgc cgctgcgtcc gctatctctt tcgccaccgt
     4141 ttgtagcgtt acgtagcgtc aatgtccgcc ttcagttgca ttttgtcagc ggtttcgtga
     4201 cgaagctcca agcggtttac gccatcaatt aaacacaaag tgctgtgcca aaactcctct
     4261 cgcttcttat ttttgtttgt tttttgagtg attggggtgg tgattggttt tgggtgggta
     4321 agcaggggaa agtgtgaaaa atcccggcaa tgggccaaga ggatcaggag ctattaattc
     4381 gcggaggcag caaacaccca tctgccgagc atctgaacaa tgtgagtagt acatgtgcat
     4441 acatcttaag ttcacttgat ctataggaac tgcgattgca acatcaaatt gtctgcggcg
     4501 tgagaactgc gacccacaaa aatcccaaac cgcaattgca caaacaaata gtgacacgaa
     4561 acagattatt ctggtagctg ttctcgctat ataagacaat ttttgagatc atatcatgat
     4621 caagacatct aaaggcattc attttcgact atattctttt ttacaaaaaa tataacaacc
     4681 agatatttta agctgatcct agatgcacaa aaaataaata aaagtataaa cctacttcgt
     4741 aggatacttc ggggtacttt ttgttcgggg ttagatgagc ataacgcttg tagttgatat
     4801 ttgagatccc ctatcattgc agggtgacag cggagcggct tcgcagagct gcattaacca
     4861 gggcttcggg caggccaaaa actacggcac gctccggcca cccagtccgc cggaggactc
     4921 cggttcaggg agcggccaac tagccgagaa cctcacctat gcctggcaca atatggacat
     4981 ctttggggcg gtcaatcagc cgggctccgg atggcggcag ctggtcaacc ggacacgcgg
     5041 actattctgc aacgagcgac acataccggc gcccaggaaa catttgctca agaacggtga
     5101 gtttctattc gcagtcggct gatctgtgtg aaatcttaat aaagggtcca attaccaatt
     5161 tgaaactcag tttgcggcgt ggcctatccg ggcgaacttt tggccgtgat gggcagttcc
     5221 ggtgccggaa agacgaccct gctgaatgcc cttgcctttc gatcgccgca gggcatccaa
     5281 gtatcgccat ccgggatgcg actgctcaat ggccaacctg tggacgccaa ggagatgcag
     5341 gccaggtgcg cctatgtcca gcaggatgac ctctttatcg gctccctaac ggccagggaa
     5401 cacctgattt tccaagccat ggtgcggatg ccacgacatc tgacctatcg gcagcgagtg
     5461 gcccgcgtgg atcaggtgat ccaggagctt tcgctcagca aatgtcagca cacgatcatc
     5521 ggtgtgcccg gcagggtgaa aggtctgtcc ggcggagaaa ggaagcgtct ggcattcgcc
     5581 tccgaggctc taaccgatcc gccgcttctg atctgcgatg agcccacctc cggactggac
     5641 tcctttaccg cccacagcgt cgtccaggtg ctgaagaagc tgtcgcagaa gggcaagacc
     5701 gtcatcctga ccattcatca gccgtcttcc gagctgtttg agctctttga caagatcctt
     5761 ctgatggccg agggcagggt agctttcttg ggcactccca gcgaagccgt cgacttcttt
     5821 tcctagtgag ttcgatgtgt ttattaaggg tatctagtat tacataacat ctcaactcct
     5881 atccagcgtg ggtgcccagt gtcctaccaa ctacaatccg gcggactttt acgtacaggt
     5941 gttggccgtt gtgcccggac gggagatcga gtcccgtgat cggatcgcca agatatgcga
     6001 caattttgcc attagcaaag tagcccggga tatggagcag ttgttggcca ccaaaaatct
     6061 ggagaagcca ctggagcagc cggagaatgg gtacacctac aaggccacct ggttcatgca
     6121 gttccgggcg gtcctgtggc gatcctggct gtcggtgctc aaggaaccac tcctcgtaaa
     6181 agtgcgactt attcagacaa cggtgagtgg ttccagtgga aacaaatgat ataacgctta
     6241 caattcttgg aaacaaattc gctagatttt agatagaatt gcctgattcc acacccttct
     6301 tagttttttt caatgagatg tatagtttat agttttgcag aagataaata aatttcattt
     6361 aactcgcgaa tattaatgag atgcgagtaa cattttaatt tgcagatggt tgccatcttg
     6421 attggcctca tctttttggg ccaacaactc acgcaagtgg gtgtgatgaa tatcaacgga
     6481 gccatcttcc tcttcctgac caacatgacc tttcaaaacg tctttgccac gataaatgta
     6541 agtcatgttt agaatacatt tgcatttcaa taatttacta actttctaat gaatcgattc
     6601 gatttaggtg ttcacctcag agctgccagt ttttatgagg gaggcccgaa gtcgacttta
     6661 tcgctgtgac acatactttc tgggcaaaac gattgccgaa ttgccgcttt ttctcacagt
     6721 gccactggtc ttcacggcga ttgcctatcc gatgatcgga ctgcgggccg gagtgctgca
     6781 cttcttcaac tgcctggcgc tggtcactct ggtggccaat gtgtcaacgt ccttcggata
     6841 tctaatatcc tgcgccagct cctcgacctc gatggcgctg tctgtgggtc cgccggttat
     6901 cataccattc ctgctctttg gcggcttctt cttgaactcg ggctcggtgc cagtatacct
     6961 caaatggttg tcgtacctct catggttccg ttacgccaac gagggtctgc tgattaacca
     7021 atgggcggac gtggagccgg gcgaaattag ctgcacatcg tcgaacacca cgtgccccag
     7081 ttcgggcaag gtcatcctgg agacgcttaa cttctccgcc gccgatctgc cgctggacta
     7141 cgtgggtctg gccattctca tcgtgagctt ccgggtgctc gcatatctgg ctctaagact
     7201 tcgggcccga cgcaaggagt agccgacata tatccgaaat aactgcttgt tttttttttt
     7261 taccattatt accatcgtgt ttactgttta ttgccccctc aaaaagctaa tgtaattata
     7321 tttgtgccaa taaaaacaag atatgaccta tagaatacaa gtatttcccc ttcgaacatc
     7381 cccacaagta gactttggat ttgtcttcta accaaaagac ttacacacct gcatacctta
     7441 catcaaaaac tcgtttatcg ctacataaaa caccgggata tattttttat atacatactt
     7501 ttcaaatcgc gcgccctctt cataattcac ctccaccaca ccacgtttcg tagttgctct
     7561 ttcgctgtct cccacccgct ctccgcaaca cattcacctt ttgttcgacg accttggagc
     7621 gactgtcgtt agttccgcgc gattcggttc gctcaaatgg ttccgagtgg ttcatttcgt
     7681 ctcaatagaa attagtaata aatatttgta tgtacaattt atttgctcca atatatttgt
     7741 atatatttcc ctcacagcta tatttattct aatttaatat tatgactttt taaggtaatt
     7801 ttttgtgacc tgttcggagt gattagcgtt acaatttgaa ctgaaagtga catccagtgt
     7861 ttgttccttg tgtagatgca tctcaaaaaa atggtgggca taatagtgtt gtttatatat
     7921 atcaaaaata acaactataa taataagaat acatttaatt tagaaaatgc ttggatttca
     7981 ctggaactag gctagcataa cttcgtataa tgtatgctat acgaagttat gctagcggat
     8041 ccaagcttgc atgcctgcag gtcggagtac tgtcctccga gcggagtact gtcctccgag
     8101 cggagtactg tcctccgagc ggagtactgt cctccgagcg gagtactgtc ctccgagcgg
     8161 agactctagc gagcgccgga gtataaatag aggcgcttcg tctacggagc gacaattcaa
     8221 ttcaaacaag caaagtgaac acgtcgctaa gcgaaagcta agcaaataaa caagcgcagc
     8281 tgaacaagct aaacaatctg cagtaaagtg caagttaaag tgaatcaatt aaaagtaacc
     8341 agcaaccaag taaatcaact gcaactactg aaatctgcca agaagtaatt attgaataca
     8401 agaagagaac tctgaatagg gaattggggc caccatggac tatggcggcg ctttgtctgc
     8461 cgtcggacgc gaacttttgt tcgttactaa tcctgtggtg gtgaacgggt ccgtcctggt
     8521 ccctgaggat caatgttact gtgccggatg gattgaatct cgcggcacga acggcgctca
     8581 gaccgcgtca aatgtcctgc agtggcttgc agcaggattc gacattttgc tgctgatgtt
     8641 ctatgcctac caaacctgga aatctacatg cggctgggag gagatctatg tgtgcgccat
     8701 tgaaatggtt aaggtgattc tcgagttctt ttttgagttt aagaatccct ctatgctcta
     8761 ccttgccaca ggacaccggg tgcagtggct gcgctatgca gagtggctgc tcacttgtcc
     8821 tgtcatctgt atccacctga gcaacctcac cggcctgagc aacgactaca gcaggagaac
     8881 catgggactc cttgtctcag acatcgggtg tatcgtgtgg ggggctacca gcgccatggc
     8941 aaccggctat gttaaagtca tcttcttttg tcttggattg tgctatggcg cgaacacatt
     9001 ttttcacgcc gccaaagcat atatcgaggg ttatcatact gtgccaaagg gtcggtgccg
     9061 ccaggtcgtg accggcatgg catggctgtt tttcgtgagc tggggtatgt tcccaattct
     9121 cttcattttg gggcccgaag gttttggcgt cctgagcgtc tatggctcca ccgtaggtca
     9181 cacgattatt gatctgatga gtaaagagtg ttgggggttg ttgggacact acctgcgcgt
     9241 cctgatccac gagcacatat tgattcacgg agatatccgc aaaaccacca aactgaacat
     9301 cggcggaacg gagatcgagg tcgagactct cgtcgaagac gaagccgagg ccggagccgt
     9361 g
//
