LOCUS       sequence-370190-        6276 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses CapChR2-mScarlet in mammalian neurons.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6276)
  AUTHORS   Fernandez Lahore RG, Pampaloni NP, Peter E, Heim MM, Tillert L, 
            Vierock J, Oppermann J, Walther J, Schmitz D, Owald D, Plested AJR, 
            Rost BR, Hegemann P
  TITLE     Calcium-permeable channelrhodopsins for the photocontrol of calcium 
            signalling.
  JOURNAL   Nat Commun. 2022 Dec 21;13(1):7844. doi: 10.1038/s41467-022-35373-4.
  PUBMED    36543773
REFERENCE   2  (bases 1 to 6276)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-12-21"; volume: "13(1):7844. doi"; pages: " 
            10.1038/s41467-022-35373-4"
COMMENT     Sequence Label: pAAV-Syn_CapChR2_mScarlet
FEATURES             Location/Qualifiers
     source          1..6276
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        51..498
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     CDS             517..1380
                     /codon_start=1
                     /product="high-photocurrent channelrhodopsin from 
                     Chloromonas oogama (Shemesh et al., 2017)"
                     /label=CoChR
                     /translation="MLGNGSAIVPIDQCFCLAWTDSLGSDTEQLVANILQWFAFGFDIL
                     ILMFYAYQTWRATCGWEEVYVCCVELTKVIIEFFHEFDDPSMLYLANGHRVQWLRYAEW
                     LLTCPVICIHLSNLTGLKDDYSKRTMRLLVSDVGCIVWGATSAMSTGYVKVIFFVLGCI
                     YGANTFFHAAKVYIESYHVVPKGRPRTVVRIMAWLFFLSWGMFPVLFVVGPEGFDAISV
                     YGSTIGHTIIDLMSKECWGLLGHYLRVLIHQHIIIYGDIRKKTKINVAGEEMEVETMVD
                     QEDEETV"
     CDS             1399..2091
                     /codon_start=1
                     /product="bright monomeric red fluorescent protein evolved 
                     from a synthetic template (Bindels et al., 2016)"
                     /label=mScarlet
                     /translation="VSKGEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFSWDILSPQFMYGSRAFTKHPADIPDYYKQSFPEGFKWERVMNFEDGGA
                     VTVTQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDGVLKGDIKM
                     ALRLKDGGRYLADFKTTYKAKKPVQMPGAYNVDRKLDITSHNEDYTVVEQYERSEGRHS
                     TGGMDELYK"
     primer_bind     complement(1529..1547)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1797..1816
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    2119..2707
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2172..2192)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2590..2601)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    2727..3203
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(2926..2945)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     repeat_region   3249..3389
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   3249..3378
                     /label=AAV2 ITR
     rep_origin      3464..3919
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3551..3570)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3761..3782
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3936..3955)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4055..4077
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4115..4133)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4201..4305
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4306..4374,4375..5166)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4524..4543)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5337..5925
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5826..5845
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     repeat_region   5987..6116
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
ORIGIN
        1 gtcggcagca aatggttaat taatctagac tgcagagggc cctgcgtatg agtgcaagtg
       61 ggttttagga ccaggatgag gcggggtggg ggtgcctacc tgacgaccga ccccgaccca
      121 ctggacaagc acccaacccc cattccccaa attgcgcatc ccctatcaga gagggggagg
      181 ggaaacagga tgcggcgagg cgcgtgcgca ctgccagctt cagcaccgcg gacagtgcct
      241 tcgcccccgc ctggcggcgc gcgccaccgc cgcctcagca ctgaaggcgc gctgacgtca
      301 ctcgccggtc ccccgcaaac tccccttccc ggccaccttg gtcgcgtccg cgccgccgcc
      361 ggcccagccg gaccgcacca cgcgaggcgc gagatagggg ggcacgggcg cgaccatctg
      421 cgctgcggcg ccggcgactc agcgctgcct cagtctgcgg tgggcagcgg aggagtcgtg
      481 tcgtgcctga gagcgcagtc gagaggatcc gccaccatgc tgggaaacgg cagcgccatt
      541 gtgcctatcg accagtgctt ttgcctggct tggaccgaca gcctgggaag cgatacagag
      601 cagctggtgg ccaacatcct ccagtggttc gccttcggct tcgacatcct gatcctgatg
      661 ttctacgcct accagacttg gagagccact tgcggttggg aggaggtcta cgtctgttgc
      721 gtcgagctga ccaaggtcat catcgagttc ttccacgagt tcgacgaccc cagcatgctg
      781 tacctggcta acggacaccg agtccagtgg ctgagatacg cagagtggct gctgacttgt
      841 cccgtcatct gcatccacct gagcaacctg accggcctga aggacgacta cagcaagcgg
      901 accatgaggc tgctggtgtc agacgtggga tgcatcgtgt ggggagctac aagcgccatg
      961 agcacaggct acgtcaaggt catcttcttc gtgctgggtt gcatctacgg cgccaacacc
     1021 ttcttccacg ccgccaaggt gtatatcgag agctaccacg tggtgccaaa gggcagacct
     1081 agaaccgtcg tgcggatcat ggcttggctg ttcttcctgt cttggggcat gttccccgtg
     1141 ctgttcgtcg tgggaccaga aggattcgac gccatcagcg tgtacggctc taccattggc
     1201 cacaccatca tcgacctcat gagcaaggag tgttggggcc tgctgggaca ctatctgaga
     1261 gtgctgatcc accagcacat catcatctac ggcgacatcc ggaagaagac caagatcaac
     1321 gtggccggcg aggagatgga agtggagacc atggtggacc aggaggacga ggagacagtg
     1381 gcggcaccgg tagtagcagt gagcaagggc gaggcagtga tcaaggagtt catgcggttc
     1441 aaggtgcaca tggagggctc catgaacggc cacgagttcg agatcgaggg cgagggcgag
     1501 ggccgcccct acgagggcac ccagaccgcc aagctgaagg tgaccaaggg tggccccctg
     1561 cccttctcct gggacatcct gtcccctcag ttcatgtacg gctccagggc cttcaccaag
     1621 caccccgccg acatccccga ctactataag cagtccttcc ccgagggctt caagtgggag
     1681 cgcgtgatga acttcgagga cggcggcgcc gtgaccgtga cccaggacac ctccctggag
     1741 gacggcaccc tgatctacaa ggtgaagctc cgcggcacca acttccctcc tgacggcccc
     1801 gtaatgcaga agaagacaat gggctgggaa gcgtccaccg agcggttgta ccccgaggac
     1861 ggcgtgctga agggcgacat taagatggcc ctgcgcctga aggacggcgg ccgctacctg
     1921 gcggacttca agaccaccta caaggccaag aagcccgtgc agatgcccgg cgcctacaac
     1981 gtcgaccgca agttggacat cacctcccac aacgaggact acaccgtggt ggaacagtac
     2041 gaacgctccg agggccgcca ctccaccggc ggcatggacg agctgtacaa gtaagaattc
     2101 cgtacaaagc ttatcgataa tcaacctctg gattacaaaa tttgtgaaag attgactggt
     2161 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat
     2221 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg
     2281 tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt
     2341 gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact
     2401 ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc
     2461 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg
     2521 tcctttcctt ggctgctcgc ctatgttgcc acctggattc tgcgcgggac gtccttctgc
     2581 tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg
     2641 cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc
     2701 tccccgcatc gataccggct cgagacgggt ggcatccctg tgacccctcc ccagtgcctc
     2761 tcctggccct ggaagttgcc actccagtgc ccaccagcct tgtcctaata aaattaagtt
     2821 gcatcatttt gtctgactag gtgtccttct ataatattat ggggtggagg ggggtggtat
     2881 ggagcaaggg gcaagttggg aagacaacct gtagggcctg cggggtctat tgggaaccaa
     2941 gctggagtgc agtggcacaa tcttggctca ctgcaatctc cgcctcctgg gttcaagcga
     3001 ttctcctgcc tcagcctccc gagttgttgg gattccaggc atgcatgacc aggctcagct
     3061 aatttttgtt tttttggtag agacggggtt tcaccatatt ggccaggctg gtctccaact
     3121 cctaatctca ggtgatctac ccaccttggc ctcccaaatt gctgggatta caggcgtgaa
     3181 ccactgctcc cttccctgtc cttctcgagc tgattttgta ggtaaccacg tgcggaccga
     3241 gcggccgcag gaacccctag tgatggagtt ggccactccc tctctgcgcg ctcgctcgct
     3301 cactgaggcc gggcgaccaa aggtcgcccg acgcccgggc tttgcccggg cggcctcagt
     3361 gagcgagcga gcgcgcagct gcctgcaggg gcgcctgatg cggtattttc tccttacgca
     3421 tctgtgcggt atttcacacc gcatacgtca aagcaaccat agtacgcgcc ctgtagcggc
     3481 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
     3541 ttagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
     3601 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
     3661 gaccccaaaa aacttgattt gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
     3721 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
     3781 ggaacaacac tcaactctat ctcgggctat tcttttgatt tataagggat tttgccgatt
     3841 tcggtctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa
     3901 atattaacgt ttacaatttt atggtgcact ctcagtacaa tctgctctga tgccgcatag
     3961 ttaagccagc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc
     4021 ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt
     4081 tcaccgtcat caccgaaacg cgcgagacga aagggcctcg tgatacgcct atttttatag
     4141 gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg
     4201 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
     4261 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat
     4321 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
     4381 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc
     4441 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca
     4501 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg
     4561 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca
     4621 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata
     4681 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag
     4741 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg
     4801 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca
     4861 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta
     4921 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct
     4981 ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca
     5041 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag
     5101 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
     5161 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
     5221 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
     5281 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     5341 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     5401 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     5461 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
     5521 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     5581 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     5641 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     5701 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     5761 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     5821 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     5881 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     5941 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgtcctg caggcagctg
     6001 cgcgctcgct cgctcactga ggccgcccgg gcgtcgggcg acctttggtc gcccggcctc
     6061 agtgagcgag cgagcgcgca gagagggagt ggccaactcc atcactaggg gttcctgcgg
     6121 ccgcacgcgt tgcaaagatg gataaagttt taaacagaga ggaatctttg cagctaatgg
     6181 accttctagg tcttgaaagg agtgggaatt ggctccggtg cccgtcagtg ggcagagcgc
     6241 acatcgccca cagtccccga gaagttgggg ggaggg
//
