LOCUS       sequence-370198-        9826 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of hTCR alpha (GPa3b17) for low expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9826)
  AUTHORS   Susac L, Vuong MT, Thomas C, von Bulow S, O'Brien-Ball C, Santos AM,
            Fernandes RA, Hummer G, Tampe R, Davis SJ
  TITLE     Structure of a fully assembled tumor-specific T cell receptor 
            ligated by pMHC.
  JOURNAL   Cell. 2022 Aug 18;185(17):3201-3213.e19. doi: 
            10.1016/j.cell.2022.07.010.
  PUBMED    35985289
REFERENCE   2  (bases 1 to 9826)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.07"; journalName: "Cell"; date: "2022-08-18- 
            18"; volume: "185"; issue: "17"; pages: "3201-3213.e19"
COMMENT     Sequence Label: pHRi_GPa3b17 TCR-alpha
FEATURES             Location/Qualifiers
     source          1..9826
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(7..18)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(127..143)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(128..144)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             234..467
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(492..510)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(493..510)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(800..819)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(1033..1052)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1152..1174
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(1212..1230)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1298..1402
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1403..1471,1472..2263)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1621..1640)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2434..3022
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2923..2942
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3176..3193
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(3263..3283)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3268..3597
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3448..3583
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          4731..4796
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             4926..4946
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    5371..5505
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5408..5427)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5462..5481
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             5673..6306
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    6353..6478
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    6975..7208
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7393..7437
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    7722..7839
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        8172..8410
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     8357..8378
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     misc_feature    join(9362..9826,1..124)
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9415..9435)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
ORIGIN
        1 gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg
       61 cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc
      121 ccgcatcgat accgtcgacc tcgagggaat taattcgagc tcggtacctt taagaccaat
      181 gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg
      241 gctaattcac tcccaacgaa gacaagatct gctttttgct tgtactgggt ctctctggtt
      301 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca
      361 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa
      421 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagca tctagaatta
      481 attccgtgta ttctatagtg tcacctaaat cgtatgtgta tgatacataa ggttatgtat
      541 taattgtagc cgcgttctaa cgacaatatg tacaagccta attgtgtagc atctggctta
      601 ctgaagcaga ccctatcatc tctctcgtaa actgccgtca gagtcggttt ggttggacga
      661 accttctgag tttctggtaa cgccgtcccg cacccggaaa tggtcagcga accaatcagc
      721 agggtcatcg ctagccagat cctctacgcc ggacgcatcg tggccggcat caccggcgcc
      781 acaggtgcgg ttgctggcgc ctatatcgcc gacatcaccg atggggaaga tcgggctcgc
      841 cacttcgggc tcatgagcgc ttgtttcggc gtgggtatgg tggcaggccc cgtggccggg
      901 ggactgttgg gcgccatctc cttgcatgca ccattccttg cggcggcggt gctcaacggc
      961 ctcaacctac tactgggctg cttcctaatg caggagtcgc ataagggaga gcgtcgaatg
     1021 gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagcccc gacacccgcc
     1081 aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc
     1141 tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc
     1201 gagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt
     1261 ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
     1321 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
     1381 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt
     1441 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga
     1501 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa
     1561 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct
     1621 gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat
     1681 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga
     1741 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc
     1801 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat
     1861 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa
     1921 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac
     1981 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa
     2041 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc
     2101 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc
     2161 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag
     2221 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta
     2281 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa
     2341 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
     2401 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     2461 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     2521 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     2581 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     2641 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     2701 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     2761 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     2821 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     2881 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     2941 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     3001 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
     3061 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg
     3121 tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga
     3181 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
     3241 gccgattcat taatgcagct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc
     3301 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga
     3361 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
     3421 accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat
     3481 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc
     3541 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag
     3601 cttggacaca agacaggctt gcgagatatg tttgagaata ccactttatc ccgcgtcagg
     3661 gagaggcagt gcgtaaaaag acgcggactc atgtgaaata ctggttttta gtgcgccaga
     3721 tctctataat ctcgcgcaac ctattttccc ctcgaacact ttttaagccg tagataaaca
     3781 ggctgggaca cttcacatga gcgaaaaata catcgtcacc tgggacatgt tgcagatcca
     3841 tgcacgtaaa ctcgcaagcc gactgatgcc ttctgaacaa tggaaaggca ttattgccgt
     3901 aagccgtggc ggtctgtacc gggtgcgtta ctggcgcgtg aactgggtat tcgtcatgtc
     3961 gataccgttt gtatttccag ctacgatcac gacaaccagc gcgagcttaa agtgctgaaa
     4021 cgcgcagaag gcgatggcga aggcttcatc gttattgatg acctggtgga taccggtggt
     4081 actgcggttg cgattcgtga aatgtatcca aaagcgcact ttgtcaccat cttcgcaaaa
     4141 ccggctggtc gtccgctggt tgatgactat gttgttgata tcccgcaaga tacctggatt
     4201 gaacagccgt gggatatggg cgtcgtattc gtcccgccaa tctccggtcg ctaatctttt
     4261 caacgcctgg cactgccggg cgttgttctt tttaacttca ggcgggttac aatagtttcc
     4321 agtaagtatt ctggaggctg catccatgac acaggcaaac ctgagcgaaa ccctgttcaa
     4381 accccgcttt aaacatcctg aaacctcgac gctagtccgc cgctttaatc acggcgcaca
     4441 accgcctgtg cagtcggccc ttgatggtaa aaccatccct cactggtatc gcatgattaa
     4501 ccgtctgatg tggatctggc gcggcattga cccacgcgaa atcctcgacg tccaggcacg
     4561 tattgtgatg agcgatgccg aacgtaccga cgatgattta tacgatacgg tgattggcta
     4621 ccgtggcggc aactggattt atgagtgggc cccggatctt tgtgaaggaa ccttacttct
     4681 gtggtgtgac ataattggac aaactaccta cagagattta aagctctaag gtaaatataa
     4741 aatttttaag tgtataatgt gttaaactac tgattctaat tgtttgtgta ttttagattc
     4801 caacctatgg aactgatgaa tgggagcagt ggtggaatgc ctttaatgag gaaaacctgt
     4861 tttgctcaga agaaatgcca tctagtgatg atgaggctac tgctgactct caacattcta
     4921 ctcctccaaa aaagaagaga aaggtagaag accccaagga ctttccttca gaattgctaa
     4981 gttttttgag tcatgctgtg tttagtaata gaactcttgc ttgctttgct atttacacca
     5041 caaaggaaaa agctgcactg ctatacaaga aaattatgga aaaatattct gtaaccttta
     5101 taagtaggca taacagttat aatcataaca tactgttttt tcttactcca cacaggcata
     5161 gagtgtctgc tattaataac tatgctcaaa aattgtgtac ctttagcttt ttaatttgta
     5221 aaggggttaa taaggaatat ttgatgtata gtgccttgac tagagatcat aatcagccat
     5281 accacatttg tagaggtttt acttgcttta aaaaacctcc cacacctccc cctgaacctg
     5341 aaacataaaa tgaatgcaat tgttgttgtt aacttgttta ttgcagctta taatggttac
     5401 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
     5461 tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggatcaactg gataactcaa
     5521 gctaaccaaa atcatcccaa acttcccacc ccatacccta ttaccactgc caattacctg
     5581 tggtttcatt tactctaaac ctgtgattcc tctgaattat tttcatttta aagaaattgt
     5641 atttgttaaa tatgtactac aaacttagta gttggaaggg ctaattcact cccaaagaag
     5701 acaagatatc cttgatctgt ggatctacca cacacaaggc tacttccctg attagcagaa
     5761 ctacacacca gggccagggg tcagatatcc actgaccttt ggatggtgct acaagctagt
     5821 accagttgag ccagataagg tagaagaggc caataaagga gagaacacca gcttgttaca
     5881 ccctgtgagc ctgcatggga tggatgaccc ggagagagaa gtgttagagt ggaggtttga
     5941 cagccgccta gcatttcatc acgtggcccg agagctgcat ccggagtact tcaagaactg
     6001 ctgatatcga gcttgctaca agggactttc cgctggggac tttccaggga ggcgtggcct
     6061 gggcgggact ggggagtggc gagccctcag atcctgcata taagcagctg ctttttgcct
     6121 gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc taactaggga
     6181 acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc
     6241 tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct
     6301 ctagcagtgg cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg
     6361 acgcaggact cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag
     6421 tacgccaaaa attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag
     6481 tattaagcgg gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa
     6541 gaaaaaatat aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt
     6601 taatcctggc ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc
     6661 atcccttcag acaggatcag aagaacttag atcattatat aatacagtag caaccctcta
     6721 ttgtgtgcat caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga
     6781 agagcaaaac aaaagtaaga ccaccgcaca gcaagcggcc ggccgctgat cttcagacct
     6841 ggaggaggag atatgaggga caattggaga agtgaattat ataaatataa agtagtaaaa
     6901 attgaaccat taggagtagc acccaccaag gcaaagagaa gagtggtgca gagagaaaaa
     6961 agagcagtgg gaataggagc tttgttcctt gggttcttgg gagcagcagg aagcactatg
     7021 ggcgcagcgt caatgacgct gacggtacag gccagacaat tattgtctgg tatagtgcag
     7081 cagcagaaca atttgctgag ggctattgag gcgcaacagc atctgttgca actcacagtc
     7141 tggggcatca agcagctcca ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa
     7201 cagctcctgg ggatttgggg ttgctctgga aaactcattt gcaccactgc tgtgccttgg
     7261 aatgctagtt ggagtaataa atctctggaa cagatttgga atcacacgac ctggatggag
     7321 tgggacagag aaattaacaa ttacacaagc ttaatacact ccttaattga agaatcgcaa
     7381 aaccagcaag aaaagaatga acaagaatta ttggaattag ataaatgggc aagtttgtgg
     7441 aattggttta acataacaaa ttggctgtgg tatataaaat tattcataat gatagtagga
     7501 ggcttggtag gtttaagaat agtttttgct gtactttcta tagtgaatag agttaggcag
     7561 ggatattcac cattatcgtt tcagacccac ctcccaaccc cgaggggacc cgacaggccc
     7621 gaaggaatag aagaagaagg tggagagaga gacagagaca gatccattcg attagtgaac
     7681 ggatctcgac ggtatcgcca aatggcagta ttcatccaca attttaaaag aaaagggggg
     7741 attggggggt acagtgcagg ggaaagaata gtagacataa tagcaacaga catacaaact
     7801 aaagaattac aaaaacaaat tacaaaaatt caaaattttc gggtttatta cagggacagc
     7861 agagatccag tttggatcga taagcttgat atcgaattcg cccttattat aaagtcgaca
     7921 gatctcggcc gcatattaag tgcattgttc tcgataccgc taagtgcatt gttctcgtta
     7981 gctcgatgga caagtgcatt gttctcttgc tgaaagctcg atggacaagt gcattgttct
     8041 cttgctgaaa gctcgatgga caagtgcatt gttctcttgc tgaaagctca gtacccgggt
     8101 cggagtactg ccccgcccct agcgattagc cccggccccg catagctccg ccccgggagt
     8161 accctcgacc gccggagtat aaatagaggc gcttcgtcta cggagcgaca attcaattca
     8221 aacaagcaaa gtgaacacgt cgctaagcga aagctaagca aataaacaag cgcagctgaa
     8281 caagctaaac aatctgcagt aaagtgcaag ttaaagtgaa tcaattaaaa gtaaccagca
     8341 accaagtaaa tcaactgcaa ctactgaaat ctgccaagaa gtaattattg aatacaagac
     8401 gagaactctg aatactttca acaagttacc gagaaagaag aactcacaca cagctagcgt
     8461 ttaaacttac gcgtgccacc atgctcctgc tgctcgtccc agtgctcgag gtgattttta
     8521 ccctgggagg aaccagagcc atgagtcaac aaggagaaga agatccgcag gccttgagca
     8581 tccaggaggg tgaaaatgcc accatgaact gcagttacaa aactagtata aacaatttac
     8641 agtggtatag acaaaattca ggtagaggcc ttgtccacct aattttaata cgttcaaatg
     8701 aaagagagaa acacagtgga agattaagag tcacgcttga cacttccaag aaaagcagtt
     8761 ccttgttgat cacggcttcc cgggcagcag acactgcttc ttacttctgt gctacggacg
     8821 gatcgactcc gatgcagttt ggaaagggca caagactttc tgtgattcca aatatccaga
     8881 accctgaccc tgccgtgtac cagctgagag actctaaatc cagtgacaag tctgtctgcc
     8941 tattcaccga ttttgattct caaacaaatg tgtcacaaag taaggattct gatgtgtata
     9001 tcacagacaa aactgtgcta gacatgaggt ctatggactt caagagcaac agtgctgtgg
     9061 cctggagcaa caaatctgac tttgcatgtg caaacgcctt caacaacagc attattccag
     9121 aagacacctt cttccccagc ccagaaagtt cctgtgatgt caagctggtc gagaaaagct
     9181 ttgaaacaga tacgaaccta aactttcaaa acctgtcagt gattgggttc cgaatcctcc
     9241 tcctgaaagt ggccgggttt aatctgctca tgacgctgcg gctgtggtcc agcggatcct
     9301 aagcggccgc gactctagag tcgacctgca ggcatgcaag cttgatatca agcttatcga
     9361 taatcaacct ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc
     9421 tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg
     9481 tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt
     9541 gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac
     9601 tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc
     9661 tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct
     9721 gttgggcact gacaattccg tggtgttgtc ggggaaatca tcgtcctttc cttggctgct
     9781 cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctac
//
