LOCUS       sequence-370201-        9856 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of hTCR alpha (1G4) for low expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9856)
  AUTHORS   Susac L, Vuong MT, Thomas C, von Bulow S, O'Brien-Ball C, Santos AM,
            Fernandes RA, Hummer G, Tampe R, Davis SJ
  TITLE     Structure of a fully assembled tumor-specific T cell receptor 
            ligated by pMHC.
  JOURNAL   Cell. 2022 Aug 18;185(17):3201-3213.e19. doi: 
            10.1016/j.cell.2022.07.010.
  PUBMED    35985289
REFERENCE   2  (bases 1 to 9856)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.07"; journalName: "Cell"; date: "2022-08-18- 
            18"; volume: "185"; issue: "17"; pages: "3201-3213.e19"
COMMENT     Sequence Label: pHRi_1G4 hTCR-alpha
FEATURES             Location/Qualifiers
     source          1..9856
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(66..82)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(67..83)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             173..406
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(431..449)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(432..449)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(739..758)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(972..991)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1091..1113
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(1151..1169)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1237..1341
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1342..1410,1411..2202)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1560..1579)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2373..2961
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2862..2881
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3115..3132
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(3202..3222)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3207..3536
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3387..3522
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          4670..4735
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             4865..4885
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    5310..5444
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5347..5366)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5401..5420
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             5612..6245
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    6292..6417
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    6914..7147
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7332..7376
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    7661..7778
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        8111..8349
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     8296..8317
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     regulatory      8414..8423
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    join(9331..9856,1..63)
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9384..9404)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9802..9813)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc
       61 cgcatcgata ccgtcgacct cgagggaatt aattcgagct cggtaccttt aagaccaatg
      121 acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg
      181 ctaattcact cccaacgaag acaagatctg ctttttgctt gtactgggtc tctctggtta
      241 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
      301 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
      361 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagcat ctagaattaa
      421 ttccgtgtat tctatagtgt cacctaaatc gtatgtgtat gatacataag gttatgtatt
      481 aattgtagcc gcgttctaac gacaatatgt acaagcctaa ttgtgtagca tctggcttac
      541 tgaagcagac cctatcatct ctctcgtaaa ctgccgtcag agtcggtttg gttggacgaa
      601 ccttctgagt ttctggtaac gccgtcccgc acccggaaat ggtcagcgaa ccaatcagca
      661 gggtcatcgc tagccagatc ctctacgccg gacgcatcgt ggccggcatc accggcgcca
      721 caggtgcggt tgctggcgcc tatatcgccg acatcaccga tggggaagat cgggctcgcc
      781 acttcgggct catgagcgct tgtttcggcg tgggtatggt ggcaggcccc gtggccgggg
      841 gactgttggg cgccatctcc ttgcatgcac cattccttgc ggcggcggtg ctcaacggcc
      901 tcaacctact actgggctgc ttcctaatgc aggagtcgca taagggagag cgtcgaatgg
      961 tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagccccg acacccgcca
     1021 acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct
     1081 gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg
     1141 agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt
     1201 tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
     1261 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
     1321 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt
     1381 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat
     1441 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag
     1501 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg
     1561 ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata
     1621 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat
     1681 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc
     1741 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg
     1801 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac
     1861 gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact
     1921 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa
     1981 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct
     2041 ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc
     2101 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga
     2161 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac
     2221 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
     2281 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
     2341 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     2401 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     2461 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
     2521 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     2581 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     2641 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     2701 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     2761 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     2821 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     2881 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     2941 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     3001 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
     3061 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag
     3121 tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg
     3181 ccgattcatt aatgcagctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct
     3241 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa
     3301 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
     3361 ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt
     3421 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct
     3481 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc
     3541 ttggacacaa gacaggcttg cgagatatgt ttgagaatac cactttatcc cgcgtcaggg
     3601 agaggcagtg cgtaaaaaga cgcggactca tgtgaaatac tggtttttag tgcgccagat
     3661 ctctataatc tcgcgcaacc tattttcccc tcgaacactt tttaagccgt agataaacag
     3721 gctgggacac ttcacatgag cgaaaaatac atcgtcacct gggacatgtt gcagatccat
     3781 gcacgtaaac tcgcaagccg actgatgcct tctgaacaat ggaaaggcat tattgccgta
     3841 agccgtggcg gtctgtaccg ggtgcgttac tggcgcgtga actgggtatt cgtcatgtcg
     3901 ataccgtttg tatttccagc tacgatcacg acaaccagcg cgagcttaaa gtgctgaaac
     3961 gcgcagaagg cgatggcgaa ggcttcatcg ttattgatga cctggtggat accggtggta
     4021 ctgcggttgc gattcgtgaa atgtatccaa aagcgcactt tgtcaccatc ttcgcaaaac
     4081 cggctggtcg tccgctggtt gatgactatg ttgttgatat cccgcaagat acctggattg
     4141 aacagccgtg ggatatgggc gtcgtattcg tcccgccaat ctccggtcgc taatcttttc
     4201 aacgcctggc actgccgggc gttgttcttt ttaacttcag gcgggttaca atagtttcca
     4261 gtaagtattc tggaggctgc atccatgaca caggcaaacc tgagcgaaac cctgttcaaa
     4321 ccccgcttta aacatcctga aacctcgacg ctagtccgcc gctttaatca cggcgcacaa
     4381 ccgcctgtgc agtcggccct tgatggtaaa accatccctc actggtatcg catgattaac
     4441 cgtctgatgt ggatctggcg cggcattgac ccacgcgaaa tcctcgacgt ccaggcacgt
     4501 attgtgatga gcgatgccga acgtaccgac gatgatttat acgatacggt gattggctac
     4561 cgtggcggca actggattta tgagtgggcc ccggatcttt gtgaaggaac cttacttctg
     4621 tggtgtgaca taattggaca aactacctac agagatttaa agctctaagg taaatataaa
     4681 atttttaagt gtataatgtg ttaaactact gattctaatt gtttgtgtat tttagattcc
     4741 aacctatgga actgatgaat gggagcagtg gtggaatgcc tttaatgagg aaaacctgtt
     4801 ttgctcagaa gaaatgccat ctagtgatga tgaggctact gctgactctc aacattctac
     4861 tcctccaaaa aagaagagaa aggtagaaga ccccaaggac tttccttcag aattgctaag
     4921 ttttttgagt catgctgtgt ttagtaatag aactcttgct tgctttgcta tttacaccac
     4981 aaaggaaaaa gctgcactgc tatacaagaa aattatggaa aaatattctg taacctttat
     5041 aagtaggcat aacagttata atcataacat actgtttttt cttactccac acaggcatag
     5101 agtgtctgct attaataact atgctcaaaa attgtgtacc tttagctttt taatttgtaa
     5161 aggggttaat aaggaatatt tgatgtatag tgccttgact agagatcata atcagccata
     5221 ccacatttgt agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga
     5281 aacataaaat gaatgcaatt gttgttgtta acttgtttat tgcagcttat aatggttaca
     5341 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     5401 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gatcaactgg ataactcaag
     5461 ctaaccaaaa tcatcccaaa cttcccaccc cataccctat taccactgcc aattacctgt
     5521 ggtttcattt actctaaacc tgtgattcct ctgaattatt ttcattttaa agaaattgta
     5581 tttgttaaat atgtactaca aacttagtag ttggaagggc taattcactc ccaaagaaga
     5641 caagatatcc ttgatctgtg gatctaccac acacaaggct acttccctga ttagcagaac
     5701 tacacaccag ggccaggggt cagatatcca ctgacctttg gatggtgcta caagctagta
     5761 ccagttgagc cagataaggt agaagaggcc aataaaggag agaacaccag cttgttacac
     5821 cctgtgagcc tgcatgggat ggatgacccg gagagagaag tgttagagtg gaggtttgac
     5881 agccgcctag catttcatca cgtggcccga gagctgcatc cggagtactt caagaactgc
     5941 tgatatcgag cttgctacaa gggactttcc gctggggact ttccagggag gcgtggcctg
     6001 ggcgggactg gggagtggcg agccctcaga tcctgcatat aagcagctgc tttttgcctg
     6061 tactgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa
     6121 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct
     6181 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc
     6241 tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga
     6301 cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt
     6361 acgccaaaaa ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt
     6421 attaagcggg ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag
     6481 aaaaaatata aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt
     6541 aatcctggcc tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca
     6601 tcccttcaga caggatcaga agaacttaga tcattatata atacagtagc aaccctctat
     6661 tgtgtgcatc aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa
     6721 gagcaaaaca aaagtaagac caccgcacag caagcggccg gccgctgatc ttcagacctg
     6781 gaggaggaga tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa
     6841 ttgaaccatt aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa
     6901 gagcagtggg aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg
     6961 gcgcagcgtc aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc
     7021 agcagaacaa tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct
     7081 ggggcatcaa gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac
     7141 agctcctggg gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga
     7201 atgctagttg gagtaataaa tctctggaac agatttggaa tcacacgacc tggatggagt
     7261 gggacagaga aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa
     7321 accagcaaga aaagaatgaa caagaattat tggaattaga taaatgggca agtttgtgga
     7381 attggtttaa cataacaaat tggctgtggt atataaaatt attcataatg atagtaggag
     7441 gcttggtagg tttaagaata gtttttgctg tactttctat agtgaataga gttaggcagg
     7501 gatattcacc attatcgttt cagacccacc tcccaacccc gaggggaccc gacaggcccg
     7561 aaggaataga agaagaaggt ggagagagag acagagacag atccattcga ttagtgaacg
     7621 gatctcgacg gtatcgccaa atggcagtat tcatccacaa ttttaaaaga aaagggggga
     7681 ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta
     7741 aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca
     7801 gagatccagt ttggatcgat aagcttgata tcgaattcgc ccttattata aagtcgacag
     7861 atctcggccg catattaagt gcattgttct cgataccgct aagtgcattg ttctcgttag
     7921 ctcgatggac aagtgcattg ttctcttgct gaaagctcga tggacaagtg cattgttctc
     7981 ttgctgaaag ctcgatggac aagtgcattg ttctcttgct gaaagctcag tacccgggtc
     8041 ggagtactgc cccgccccta gcgattagcc ccggccccgc atagctccgc cccgggagta
     8101 ccctcgaccg ccggagtata aatagaggcg cttcgtctac ggagcgacaa ttcaattcaa
     8161 acaagcaaag tgaacacgtc gctaagcgaa agctaagcaa ataaacaagc gcagctgaac
     8221 aagctaaaca atctgcagta aagtgcaagt taaagtgaat caattaaaag taaccagcaa
     8281 ccaagtaaat caactgcaac tactgaaatc tgccaagaag taattattga atacaagacg
     8341 agaactctga atactttcaa caagttaccg agaaagaaga actcacacac agctagcgtt
     8401 taaacttacg cgtgccacca tggggatact tcccagccct gggatgcctg cgctgctctc
     8461 cctcgtgagc cttctctccg tgctgctgat gggttgcgta gctaaacagg aggtgacgca
     8521 gattcctgca gctctgagtg tcccagaagg agaaaacttg gttctcaact gcagtttcac
     8581 tgatagcgct atttacaacc tccagtggtt taggcaggac cctgggaaag gtctcacatc
     8641 tctgttgctt attcagtcaa gtcagagaga gcaaacaagt ggaagactta atgcctcgct
     8701 ggataaatca tcaggacgta gtactttata cattgcagct tctcagcctg gtgactcagc
     8761 cacctacctc tgtgctgtga ggcccacatc aggaggaagc tacataccta catttggaag
     8821 aggaaccagc cttattgttc atccgtatat ccagaaccct gaccctgccg tgtaccagct
     8881 gagagactct aaatccagtg acaagtctgt ctgcctattc accgattttg attctcaaac
     8941 aaatgtgtca caaagtaagg attctgatgt gtatatcaca gacaaaactg tgctagacat
     9001 gaggtctatg gacttcaaga gcaacagtgc tgtggcctgg agcaacaaat ctgactttgc
     9061 atgtgcaaac gccttcaaca acagcattat tccagaagac accttcttcc ccagcccaga
     9121 aagttcctgt gatgtcaagc tggtcgagaa aagctttgaa acagatacga acctaaactt
     9181 tcaaaacctg tcagtgattg ggttccgaat cctcctcctg aaagtggccg ggtttaatct
     9241 gctcatgacg ctgcggctgt ggtccagctg agcggccgcg actctagagt cgacctgcag
     9301 gcatgcaagc ttgatatcaa gcttatcgat aatcaacctc tggattacaa aatttgtgaa
     9361 agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta
     9421 atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa
     9481 tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg
     9541 tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc
     9601 ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc
     9661 cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg
     9721 gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg
     9781 acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg
     9841 ctgccggctc tgcggc
//
