LOCUS       sequence-370202-       10558 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of hTCR beta(1G4), and SNAP for low 
            expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10558)
  AUTHORS   Susac L, Vuong MT, Thomas C, von Bulow S, O'Brien-Ball C, Santos AM,
            Fernandes RA, Hummer G, Tampe R, Davis SJ
  TITLE     Structure of a fully assembled tumor-specific T cell receptor 
            ligated by pMHC.
  JOURNAL   Cell. 2022 Aug 18;185(17):3201-3213.e19. doi: 
            10.1016/j.cell.2022.07.010.
  PUBMED    35985289
REFERENCE   2  (bases 1 to 10558)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.07"; journalName: "Cell"; date: "2022-08-18- 
            18"; volume: "185"; issue: "17"; pages: "3201-3213.e19"
COMMENT     Sequence Label: pHRi_1G4 hTCR-beta-SNAP
FEATURES             Location/Qualifiers
     source          1..10558
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             95..328
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(353..371)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(354..371)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(661..680)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(894..913)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1013..1035
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(1073..1091)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1159..1263
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1264..1332,1333..2124)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1482..1501)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2295..2883
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2784..2803
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3037..3054
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(3124..3144)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3129..3458
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3309..3444
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          4592..4657
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             4787..4807
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    5232..5366
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5269..5288)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5323..5342
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             5534..6167
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    6214..6339
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    6836..7069
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7254..7298
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    7583..7700
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        8033..8271
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     8218..8239
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     regulatory      8336..8345
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             9320..9865
                     /codon_start=1
                     /product="human O6-alkylguanine-DNA-alkyltransferase 
                     variant that forms covalent bonds with benzylguanine 
                     derivatives"
                     /label=SNAP-tag(R)
                     /note="fast-reacting variant, optimized for mammalian 
                     expression"
                     /translation="MDKDCEMKRTTLDSPLGKLELSGCEQGLHRIIFLGKGTSAADAVE
                     VPAPAAVLGGPEPLMQATAWLNAYFHQPEAIEEFPVPALHHPVFQQESFTRQVLWKLLK
                     VVKFGEVISYSHLAALAGNPAATAAVKTALSGNPVPILIPCHRVVQGDLDVGGYEGGLA
                     VKEWLLAHEGHRLGKPGLG"
     misc_feature    9955..10543
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(10008..10028)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(10426..10437)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(join(10546..10558,1..4))
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(join(10547..10558,1..5))
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
ORIGIN
        1 ctcgagggaa ttaattcgag ctcggtacct ttaagaccaa tgacttacaa ggcagctgta
       61 gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga
      121 agacaagatc tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg
      181 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg
      241 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc
      301 ttttagtcag tgtggaaaat ctctagcagc atctagaatt aattccgtgt attctatagt
      361 gtcacctaaa tcgtatgtgt atgatacata aggttatgta ttaattgtag ccgcgttcta
      421 acgacaatat gtacaagcct aattgtgtag catctggctt actgaagcag accctatcat
      481 ctctctcgta aactgccgtc agagtcggtt tggttggacg aaccttctga gtttctggta
      541 acgccgtccc gcacccggaa atggtcagcg aaccaatcag cagggtcatc gctagccaga
      601 tcctctacgc cggacgcatc gtggccggca tcaccggcgc cacaggtgcg gttgctggcg
      661 cctatatcgc cgacatcacc gatggggaag atcgggctcg ccacttcggg ctcatgagcg
      721 cttgtttcgg cgtgggtatg gtggcaggcc ccgtggccgg gggactgttg ggcgccatct
      781 ccttgcatgc accattcctt gcggcggcgg tgctcaacgg cctcaaccta ctactgggct
      841 gcttcctaat gcaggagtcg cataagggag agcgtcgaat ggtgcactct cagtacaatc
      901 tgctctgatg ccgcatagtt aagccagccc cgacacccgc caacacccgc tgacgcgccc
      961 tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc
     1021 tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg
     1081 atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc
     1141 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
     1201 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
     1261 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt
     1321 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt
     1381 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc
     1441 cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta
     1501 tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac
     1561 ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa
     1621 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg
     1681 atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc
     1741 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg
     1801 atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta
     1861 gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg
     1921 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg
     1981 tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc
     2041 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt
     2101 gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt
     2161 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
     2221 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
     2281 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     2341 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     2401 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag
     2461 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     2521 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     2581 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     2641 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
     2701 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     2761 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     2821 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     2881 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
     2941 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
     3001 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg
     3061 gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc
     3121 tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta
     3181 tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag
     3241 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa
     3301 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac
     3361 taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt
     3421 agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gcttggacac aagacaggct
     3481 tgcgagatat gtttgagaat accactttat cccgcgtcag ggagaggcag tgcgtaaaaa
     3541 gacgcggact catgtgaaat actggttttt agtgcgccag atctctataa tctcgcgcaa
     3601 cctattttcc cctcgaacac tttttaagcc gtagataaac aggctgggac acttcacatg
     3661 agcgaaaaat acatcgtcac ctgggacatg ttgcagatcc atgcacgtaa actcgcaagc
     3721 cgactgatgc cttctgaaca atggaaaggc attattgccg taagccgtgg cggtctgtac
     3781 cgggtgcgtt actggcgcgt gaactgggta ttcgtcatgt cgataccgtt tgtatttcca
     3841 gctacgatca cgacaaccag cgcgagctta aagtgctgaa acgcgcagaa ggcgatggcg
     3901 aaggcttcat cgttattgat gacctggtgg ataccggtgg tactgcggtt gcgattcgtg
     3961 aaatgtatcc aaaagcgcac tttgtcacca tcttcgcaaa accggctggt cgtccgctgg
     4021 ttgatgacta tgttgttgat atcccgcaag atacctggat tgaacagccg tgggatatgg
     4081 gcgtcgtatt cgtcccgcca atctccggtc gctaatcttt tcaacgcctg gcactgccgg
     4141 gcgttgttct ttttaacttc aggcgggtta caatagtttc cagtaagtat tctggaggct
     4201 gcatccatga cacaggcaaa cctgagcgaa accctgttca aaccccgctt taaacatcct
     4261 gaaacctcga cgctagtccg ccgctttaat cacggcgcac aaccgcctgt gcagtcggcc
     4321 cttgatggta aaaccatccc tcactggtat cgcatgatta accgtctgat gtggatctgg
     4381 cgcggcattg acccacgcga aatcctcgac gtccaggcac gtattgtgat gagcgatgcc
     4441 gaacgtaccg acgatgattt atacgatacg gtgattggct accgtggcgg caactggatt
     4501 tatgagtggg ccccggatct ttgtgaagga accttacttc tgtggtgtga cataattgga
     4561 caaactacct acagagattt aaagctctaa ggtaaatata aaatttttaa gtgtataatg
     4621 tgttaaacta ctgattctaa ttgtttgtgt attttagatt ccaacctatg gaactgatga
     4681 atgggagcag tggtggaatg cctttaatga ggaaaacctg ttttgctcag aagaaatgcc
     4741 atctagtgat gatgaggcta ctgctgactc tcaacattct actcctccaa aaaagaagag
     4801 aaaggtagaa gaccccaagg actttccttc agaattgcta agttttttga gtcatgctgt
     4861 gtttagtaat agaactcttg cttgctttgc tatttacacc acaaaggaaa aagctgcact
     4921 gctatacaag aaaattatgg aaaaatattc tgtaaccttt ataagtaggc ataacagtta
     4981 taatcataac atactgtttt ttcttactcc acacaggcat agagtgtctg ctattaataa
     5041 ctatgctcaa aaattgtgta cctttagctt tttaatttgt aaaggggtta ataaggaata
     5101 tttgatgtat agtgccttga ctagagatca taatcagcca taccacattt gtagaggttt
     5161 tacttgcttt aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa
     5221 ttgttgttgt taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
     5281 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
     5341 tcaatgtatc ttatcatgtc tggatcaact ggataactca agctaaccaa aatcatccca
     5401 aacttcccac cccataccct attaccactg ccaattacct gtggtttcat ttactctaaa
     5461 cctgtgattc ctctgaatta ttttcatttt aaagaaattg tatttgttaa atatgtacta
     5521 caaacttagt agttggaagg gctaattcac tcccaaagaa gacaagatat ccttgatctg
     5581 tggatctacc acacacaagg ctacttccct gattagcaga actacacacc agggccaggg
     5641 gtcagatatc cactgacctt tggatggtgc tacaagctag taccagttga gccagataag
     5701 gtagaagagg ccaataaagg agagaacacc agcttgttac accctgtgag cctgcatggg
     5761 atggatgacc cggagagaga agtgttagag tggaggtttg acagccgcct agcatttcat
     5821 cacgtggccc gagagctgca tccggagtac ttcaagaact gctgatatcg agcttgctac
     5881 aagggacttt ccgctgggga ctttccaggg aggcgtggcc tgggcgggac tggggagtgg
     5941 cgagccctca gatcctgcat ataagcagct gctttttgcc tgtactgggt ctctctggtt
     6001 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca
     6061 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa
     6121 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac
     6181 agggacttga aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct
     6241 gaagcgcgca cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact
     6301 agcggaggct agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt
     6361 agatcgcgat gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa
     6421 catatagtat gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa
     6481 acatcagaag gctgtagaca aatactggga cagctacaac catcccttca gacaggatca
     6541 gaagaactta gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata
     6601 gagataaaag acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag
     6661 accaccgcac agcaagcggc cggccgctga tcttcagacc tggaggagga gatatgaggg
     6721 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     6781 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     6841 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg tcaatgacgc
     6901 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     6961 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     7021 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     7081 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     7141 aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga gaaattaaca
     7201 attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa gaaaagaatg
     7261 aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt aacataacaa
     7321 attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta ggtttaagaa
     7381 tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca ccattatcgt
     7441 ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata gaagaagaag
     7501 gtggagagag agacagagac agatccattc gattagtgaa cggatctcga cggtatcgcc
     7561 aaatggcagt attcatccac aattttaaaa gaaaaggggg gattgggggg tacagtgcag
     7621 gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa
     7681 ttacaaaaat tcaaaatttt cgggtttatt acagggacag cagagatcca gtttggatcg
     7741 ataagcttga tatcgaattc gcccttatta taaagtcgac agatctcggc cgcatattaa
     7801 gtgcattgtt ctcgataccg ctaagtgcat tgttctcgtt agctcgatgg acaagtgcat
     7861 tgttctcttg ctgaaagctc gatggacaag tgcattgttc tcttgctgaa agctcgatgg
     7921 acaagtgcat tgttctcttg ctgaaagctc agtacccggg tcggagtact gccccgcccc
     7981 tagcgattag ccccggcccc gcatagctcc gccccgggag taccctcgac cgccggagta
     8041 taaatagagg cgcttcgtct acggagcgac aattcaattc aaacaagcaa agtgaacacg
     8101 tcgctaagcg aaagctaagc aaataaacaa gcgcagctga acaagctaaa caatctgcag
     8161 taaagtgcaa gttaaagtga atcaattaaa agtaaccagc aaccaagtaa atcaactgca
     8221 actactgaaa tctgccaaga agtaattatt gaatacaaga cgagaactct gaatactttc
     8281 aacaagttac cgagaaagaa gaactcacac acagctagcg tttaaactta cgcgtgccac
     8341 catggggata cttcccagcc ctgggatgcc tgcgctgctc tccctcgtga gccttctctc
     8401 cgtgctgctg atgggttgcg tagctgctgg tgtcactcag accccaaaat tccaggtcct
     8461 gaagacagga cagagcatga cactgcagtg tgcccaggat atgaaccatg aatacatgtc
     8521 ctggtatcga caagacccag gcatggggct gaggctgatt cattactcag ttggtgctgg
     8581 tatcactgac caaggagaag tccccaatgg ctacaatgtc tccagatcaa ccacagagga
     8641 tttcccgctc aggctgctgt cggctgctcc ctcccagaca tctgtgtact tctgtgccag
     8701 cagttacgtc gggaacaccg gggagctgtt ttttggagaa ggctctaggc tgaccgtact
     8761 ggaggacctg aaaaacgtgt tcccacccga ggtcgctgtg tttgagccat cagaagcaga
     8821 gatctcccac acccaaaagg ccacactggt gtgcctggcc acaggcttct accccgacca
     8881 cgtggagctg agctggtggg tgaatgggaa ggaggtgcac agtggggtca gcacagaccc
     8941 gcagcccctc aaggagcagc ccgccctcaa tgactccaga tactgcctga gcagccgcct
     9001 gagggtctcg gccaccttct ggcagaaccc ccgcaaccac ttccgctgtc aagtccagtt
     9061 ctacgggctc tcggagaatg acgagtggac ccaggatagg gccaaacctg tcacccagat
     9121 cgtcagcgcc gaggcctggg gtagagcaga ctgtggcttc acctccgagt cttaccagca
     9181 aggggtcctg tctgccacca tcctctatga gatcttgcta gggaaggcca ccttgtatgc
     9241 cgtgctggtc agtgccctcg tgctgatggc catggtcaag agaaaggatt ccagaggcgg
     9301 aagcggaagc ggaagcggaa tggacaaaga ctgcgaaatg aagcgcacca ccctggatag
     9361 ccctctgggc aagctggaac tgtctgggtg cgaacagggc ctgcaccgta tcatcttcct
     9421 gggcaaagga acatctgccg ccgacgccgt ggaagtgcct gccccagccg ccgtgctggg
     9481 cggaccagag ccactgatgc aggccaccgc ctggctcaac gcctactttc accagcctga
     9541 ggccatcgag gagttccctg tgccagccct gcaccaccca gtgttccagc aggagagctt
     9601 tacccgccag gtgctgtgga aactgctgaa agtggtgaag ttcggagagg tcatcagcta
     9661 cagccacctg gccgccctgg ccggcaatcc cgccgccacc gccgccgtga aaaccgccct
     9721 gagcggaaat cccgtgccca ttctgatccc ctgccaccgg gtggtgcagg gcgacctgga
     9781 cgtggggggc tacgagggcg ggctcgccgt gaaagagtgg ctgctggccc acgagggcca
     9841 cagactgggc aagcctgggc tgggttaggt caagagaaag gatttcggat cctaggcggc
     9901 cgcgactcta gagtcgacct gcaggcatgc aagcttgata tcaagcttat cgataatcaa
     9961 cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt
    10021 acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct
    10081 ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc
    10141 gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg
    10201 ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc
    10261 acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc
    10321 actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt
    10381 gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca
    10441 gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt
    10501 cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcatcgata ccgtcgac
//
