LOCUS       sequence-370203-        7370 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For expression of human ATG13(Y118D).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7370)
  AUTHORS   Ren X, Nguyen TN, Lam WK, Buffalo CZ, Lazarou M, Yokom AL, Hurley JH
  TITLE     Structural basis for ATG9A recruitment to the ULK1 complex in 
            mitophagy initiation.
  JOURNAL   Sci Adv. 2023 Feb 15;9(7):eadg2997. doi: 10.1126/sciadv.adg2997. 
            Epub 2023 Feb 15.
  PUBMED    36791199
REFERENCE   2  (bases 1 to 7370)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2023-02-15"; volume: "9(7):eadg2997. doi"; pages: " 
            10.1126/sciadv.adg2997. Epub 2023 Feb 15"
COMMENT     Sequence Label: pBMN HA-ATG13(Y118D)
FEATURES             Location/Qualifiers
     source          1..7370
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(236..253)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(407..995)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(487..506)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1159..1950,1951..2019))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1782..1801
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2020..2124)
                     /gene="bla"
                     /label=AmpR promoter
     mobile_element  2269..3036
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
     LTR             3438..3907
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    3972..4329
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             4394..4810
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     4740..4762
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     4778..4794
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     misc_feature    4820..5194
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     5132..5148
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     CDS             5206..5232
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     LTR             join(7007..7370,1..106)
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
ORIGIN
        1 cgtgtatcca ataaaccctc ttgcagttgc atccgacttg tggtctcgct gttccttggg
       61 agggtctcct ctgagtgatt gactacccgt cagcgggggt ctttcacatg cagcatgtat
      121 caaaattaat ttggtttttt ttcttaagta tttacattaa atggccatag ttgcattaat
      181 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
      241 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
      301 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
      361 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
      421 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
      481 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
      541 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
      601 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
      661 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
      721 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
      781 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
      841 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
      901 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
      961 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     1021 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     1081 aaaggatctt cacctagatc cttttgcggc cggccgcaaa tcaatctaaa gtatatatga
     1141 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     1201 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
     1261 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
     1321 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
     1381 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
     1441 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
     1501 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
     1561 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
     1621 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
     1681 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
     1741 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
     1801 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
     1861 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
     1921 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
     1981 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
     2041 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
     2101 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccaccag ctttgctctt
     2161 aggagtttcc taatacatcc caaactcaaa tatataaagc atttgacttg ttctatgccc
     2221 tagggggcgg ggggaagcta agccagcttt ttttaacatt taaaatgtgg taatgactcc
     2281 aacttattga tagtgtttta tgttcagata atgcccgatg actttgtcat gcagctccac
     2341 cgattttgag aacgacagcg acttccgtcc cagccgtgcc aggtgctgcc tcagattcag
     2401 gttatgccgc tcaattcgct gcgtatatcg cttgctgatt acgtgcagct ttcccttcag
     2461 gcgggattca tacagcggcc agccatccgt catccatatc accacgtcaa agggtgacag
     2521 caggctcata agacgcccca gcgtcgccat agtgcgttca ccgaatacgt gcgcaacaac
     2581 cgtcttccgg agactgtcat acgcgtaaaa cagccagcgc tggcgcgatt tagccccgac
     2641 atagccccac tgttcgtcca tttccgcgca gacgatgacg tcactgcccg gctgtatgcg
     2701 cgaggttacc gactgcggcc tgagtttttt aagtgacgta aaatcgtgtt gaggccaacg
     2761 cccataatgc gggctgttgc ccggcatcca acgccattca tggccatatc aatgattttc
     2821 tggtgcgtac cgggttgaga agcggtgtaa gtgaactgca gttgccatgt tttacggcag
     2881 tgagagcaga gatagcgctg atgtccggcg gtgcttttgc cgttacgcac caccccgtca
     2941 gtagctgaac aggagggaca gctgatagaa acagaagcca ctggagcacc tcaaaaacac
     3001 catcatacac taaatcagta agttggcagc atcaccttaa aatgttaatt ccattttaaa
     3061 tgcacagatg tttttatttc ataagggttt caatgtgcat gaatgctgca atattcctgt
     3121 taccaaagct agtataaata aaaatagata aacgtggaaa ttacttagag tttctgtcat
     3181 taacgtttcc ttcctcagtt gacaacataa atgcgctgct gagaagccag tttgcatctg
     3241 tcaggatcaa tttcccatta tgccagtcat attaattact agtcaattag ttgattttta
     3301 tttttgacat atacatgtga aagaccccac ctgtaggttt ggcaagctag cttaagtaac
     3361 gccattttgc aaggcatgga aaaatacata actgagaata gaaaagttca gatcaaggtc
     3421 aggaacagat ggaacagctg aatatgggcc aaacaggata tctgtggtaa gcagttcctg
     3481 ccccggctca gggccaagaa cagatggaac agctgaatat gggccaaaca ggatatctgt
     3541 ggtaagcagt tcctgccccg gctcagggcc aagaacagat ggtccccaga tgcggtccag
     3601 ccctcagcag tttctagaga accatcagat gtttccaggg tgccccaagg acctgaaatg
     3661 accctgtgcc ttatttgaac taaccaatca gttcgcttct cgcttctgtt cgcgcgcttc
     3721 tgctccccga gctcaataaa agagcccaca acccctcact cggcgcgcca gtcctccgat
     3781 tgactgagtc gcccgggtac ccgtgtatcc aataaaccct cttgcagttg catccgactt
     3841 gtggtctcgc tgttccttgg gagggtctcc tctgagtgat tgactacccg tcagcggggg
     3901 tctttcattt gggggctcgt ccgggatcgg gagacccctg cccagggacc accgacccac
     3961 caccgggagg taagctggcc agcaacttat ctgtgtctgt ccgattgtct agtgtctatg
     4021 actgatttta tgcgcctgcg tcggtactag ttagctaact agctctgtat ctggcggacc
     4081 cgtggtggaa ctgacgagtt cggaacaccc ggccgcaacc ctgggagacg tcccagggac
     4141 ttcgggggcc gtttttgtgg cccgacctga gtccaaaaat cccgatcgtt ttggactctt
     4201 tggtgcaccc cccttagagg agggatatgt ggttctggta ggagacgaga acctaaaaca
     4261 gttcccgcct ccgtctgaat ttttgctttc ggtttgggac cgaagccgcg ccgcgcgtct
     4321 tgtctgctgc agcatcgttc tgtgttgtct ctgtctgact gtgtttctgt atttgtctga
     4381 aaatatgggc ccgggccaga ctgttaccac tcccttaagt ttgaccttag gtcactggaa
     4441 agatgtcgag cggatcgctc acaaccagtc ggtagatgtc aagaagagac gttgggttac
     4501 cttctgctct gcagaatggc caacctttaa cgtcggatgg ccgcgagacg gcacctttaa
     4561 ccgagacctc atcacccagg ttaagatcaa ggtcttttca cctggcccgc atggacaccc
     4621 agaccaggtc ccctacatcg tgacctggga agccttggct tttgaccccc ctccctgggt
     4681 caagcccttt gtacacccta agcctccgcc tcctcttcct ccatccgccc cgtctctccc
     4741 ccttgaacct cctcgttcga ccccgcctcg atcctccctt tatccagccc tcactccttc
     4801 tctaggcgcc cccatatggc catatgagat cttatatggg gcacccccgc cccttgtaaa
     4861 cttccctgac cctgacatga caagagttac taacagcccc tctctccaag ctcacttaca
     4921 ggctctctac ttagtccagc acgaagtctg gagacctctg gcggcagcct accaagaaca
     4981 actggaccga ccggtggtac ctcaccctta ccgagtcggc gacacagtgt gggtccgccg
     5041 acaccagact aagaacctag aacctcgctg gaaaggacct tacacagtcc tgctgaccac
     5101 ccccaccgcc ctcaaagtag acggcatcgc agcttggata cacgccgccc acgtgaaggc
     5161 tgccgacccc gggggtggac catcctctag actgccgcca ccatgtaccc atacgatgtt
     5221 ccagattacg cttccggact cagatctcga gctcaagctt cggaaactga tctcaattcc
     5281 caggacagaa aggacctgga caagtttatt aaattttttg ccctcaagac tgtccaagtg
     5341 attgtccagg ctcggcttgg tgaaaagatt tgcactcgtt catcatcttc tccaacgggt
     5401 tcagattggt tcaacttagc aatcaaagac atcccagagg ttacacatga agcaaagaag
     5461 gcactggcag gacagctgcc tgcagtcggg aggtccatgt gtgtggagat ttcacttaag
     5521 acttctgagg gagattccat ggagctggaa atatggtgtc ttgaaatgaa tgaaaagtgt
     5581 gataaagaaa tcaaagtttc ctacacggtg gacaacagac tgtcattgct gctgaagtcc
     5641 cttcttgcta taactagggt gacaccagcc tataggctct ccaggaaaca agggcatgaa
     5701 tatgtcatat tatacaggat atattttgga gaagttcagc tgagtggctt aggagaaggc
     5761 ttccagacag ttcgtgttgg gacagtgggc acccctgtgg gcaccatcac tctttcttgt
     5821 gcttacagaa ttaacttggc attcatgtct accaggcaat ttgagaggac cccacctatc
     5881 atggggatta ttattgatca ctttgtggac cgtccctatc ccagctcctc tcccatgcac
     5941 ccctgcaatt acagaactgc tggtgaggac actggagtaa tatacccgtc tgtagaagac
     6001 tctcaagaag tgtgtaccac ctctttttcc acctccccac catcccagct ctcaagctct
     6061 cgcctttcct atcagcctgc tgccctgggc gttggatcag ctgacctggc ttatccagta
     6121 gtgtttgctg ctggcttaaa tgctacacac cctcaccagc tgatggttcc tgggaaggaa
     6181 ggtggggtac cccttgctcc caaccagcct gtccatggta cccaggctga ccaggagaga
     6241 ctggcaacct gcaccccttc tgacagaacc cactgtgctg ccacaccctc cagtagtgag
     6301 gatactgaaa ccgtatcaaa cagcagtgag ggacgggcct cccctcacga tgtcttggag
     6361 accatctttg tccgaaaagt gggggctttt gtcaacaaac ccattaacca ggtgaccctg
     6421 acgagtttgg atataccctt tgccatgttt gctcccaaga atttggagct ggaggatacc
     6481 gatccaatgg tgaatcctcc agattcccca gagactgaat ctcctctcca gggcagcctg
     6541 cactcagatg gctccagcgg gggcagcagt ggcaataccc atgatgactt tgttatgata
     6601 gactttaaac cagctttttc taaagatgac attcttccga tggacctggg gaccttctat
     6661 cgggagtttc agaacccacc tcagctgagc agcctctcca tagatattgg agcacagtcc
     6721 atggctgaag acttggactc attaccagag aagctggctg tgcatgagaa gaatgtccgc
     6781 gagtttgatg cctttgtgga aaccctgcag taaggatcca ccggatctag ataactgatc
     6841 acgataaaat aaaagatttt atttagtctc cagaaaaagg ggggaatgaa agaccccacc
     6901 tgtaggtttg gcaagctagc ttaagtaacg ccattttgca aggcatggaa aaatacataa
     6961 ctgagaatag agaagttcag atcaaggtca ggaacagatg gaacagctga atatgggcca
     7021 aacaggatat ctgtggtaag cagttcctgc cccggctcag ggccaagaac agatggaaca
     7081 gctgaatatg ggccaaacag gatatctgtg gtaagcagtt cctgccccgg ctcagggcca
     7141 agaacagatg gtccccagat gcggtccagc cctcagcagt ttctagagaa ccatcagatg
     7201 tttccagggt gccccaagga cctgaaatga ccctgtgcct tatttgaact aaccaatcag
     7261 ttcgcttctc gcttctgttc gcgcgcttct gctccccgag ctcaataaaa gagcccacaa
     7321 cccctcactc ggggcgccag tcctccgatt gactgagtcg cccgggtacc
//
