LOCUS       sequence-370204-        9252 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  IRF3 - Viral response gene reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9252)
  AUTHORS   O'Connell DJ, Kolde R, Sooknah M, Graham DB, Sundberg TB, Latorre 
            IJ, Mikkelsen TS, Xavier RJ
  TITLE     Simultaneous Pathway Activity Inference and Gene Expression Analysis
            Using RNA Sequencing.
  JOURNAL   Cell Syst. 2016 May 25;2(5):323-34. doi: 10.1016/j.cels.2016.04.011.
            Epub 2016 May 19.
  PUBMED    27211859
REFERENCE   2  (bases 1 to 9252)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cels.2016.04.011"; journalName: "Cell Syst"; date: 
            "2016-05-25- 25"; volume: "2"; issue: "5"; pages: "323-34"
COMMENT     Sequence Label: pLminP_Luc2P_RE10
FEATURES             Location/Qualifiers
     source          1..9252
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        3..229
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             230..410
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    457..582
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1075..1308
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1493..1537
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1804..1921
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2084..2115
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     regulatory      2142..2151
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2148..3797
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     CDS             3801..3920
                     /codon_start=1
                     /product="PEST degradation sequence from mouse ornithine 
                     decarboxylase"
                     /label=hPEST
                     /note="human codon-optimized"
                     /translation="SHGFPPEVEEQAAGTLPMSCAQESGMDRHPAACASARINV"
     misc_feature    4012..4600
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4065..4085)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4483..4494)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        4615..4931
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4782..4917
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             join(4982..4984,4985..4987,4988..5701)
                     /codon_start=1
                     /product="Emerald GFP"
                     /label=EmGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5027..5048)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5288..5307)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5635..5656
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     LTR             5773..6006
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6078..6199
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6115..6134)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6169..6188
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6239..6374
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6394..6413)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6395..6413)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6423..6440)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6423..6439)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6432..6454)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6581..7036
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6668..6687)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6878..6899
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7062..7166
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7167..7235,7236..8027)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7385..7404)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8198..8786
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8687..8706
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8940..8957
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9074..9095
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9110..9115,9116..9133,9134..9140)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9148..9164
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9153..9175
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9172..9188
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9172..9188
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9207..9227
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9209..9227
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
       61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
      181 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
      241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      361 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
      421 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
      481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
      541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
      601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
      661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
      721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
      781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
      841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
      901 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
      961 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1021 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1081 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1141 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1201 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1261 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1321 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1381 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatcgc accagcgtgt ggatcatagg aaaactgaaa gggagaagta taggaaaact
     1981 gaaagggaga agtataggaa aactgaaagg gagaagtata ggaaaactga aagggagaag
     2041 tgatccgaga acagatctgg cctcggcggc caagcttaga cactagaggg tatataatgg
     2101 aagctcgact tccagcttgg caatccggta ctgttggtaa agccaccatg gaagatgcca
     2161 aaaacattaa gaagggccca gcgccattct acccactcga agacgggacc gccggcgagc
     2221 agctgcacaa agccatgaag cgctacgccc tggtgcccgg caccatcgcc tttaccgacg
     2281 cacatatcga ggtggacatt acctacgccg agtacttcga gatgagcgtt cggctggcag
     2341 aagctatgaa gcgctatggg ctgaatacaa accatcggat cgtggtgtgc agcgagaata
     2401 gcttgcagtt cttcatgccc gtgttgggtg ccctgttcat cggtgtggct gtggccccag
     2461 ctaacgacat ctacaacgag cgcgagctgc tgaacagcat gggcatcagc cagcccaccg
     2521 tcgtattcgt gagcaagaaa gggctgcaaa agatcctcaa cgtgcaaaag aagctaccga
     2581 tcatacaaaa gatcatcatc atggatagca agaccgacta ccagggcttc caaagcatgt
     2641 acaccttcgt gacttcccat ttgccacccg gcttcaacga gtacgacttc gtgcccgaga
     2701 gcttcgaccg ggacaaaacc atcgccctga tcatgaacag tagtggcagt accggattgc
     2761 ccaagggcgt agccctaccg caccgcaccg cttgtgtccg attcagtcat gcccgcgacc
     2821 ccatcttcgg caaccagatc atccccgaca ccgctatcct cagcgtggtg ccatttcacc
     2881 acggcttcgg catgttcacc acgctgggct acttgatctg cggctttcgg gtcgtgctca
     2941 tgtaccgctt cgaggaggag ctattcttgc gcagcttgca agactataag attcaatctg
     3001 ccctgctggt gcccacacta tttagcttct tcgctaagag cactctcatc gacaagtacg
     3061 acctaagcaa cttgcacgag atcgccagcg gcggggcgcc gctcagcaag gaggtaggtg
     3121 aggccgtggc caaacgcttc cacctaccag gcatccgcca gggctacggc ctgacagaaa
     3181 caaccagcgc cattctgatc acccccgaag gggacgacaa gcctggcgca gtaggcaagg
     3241 tggtgccctt cttcgaggct aaggtggtgg acttggacac cggtaagaca ctgggtgtga
     3301 accagcgcgg cgagctgtgc gtccgtggcc ccatgatcat gagcggctac gttaacaacc
     3361 ccgaggctac aaacgctctc atcgacaagg acggctggct gcacagcggc gacatcgcct
     3421 actgggacga ggacgagcac ttcttcatcg tggaccggct gaagagcctg atcaaataca
     3481 agggctacca ggtagcccca gccgaactgg agagcatcct gctgcaacac cccaacatct
     3541 tcgacgccgg ggtcgccggc ctgcccgacg acgatgccgg cgagctgccc gccgcagtcg
     3601 tcgtgctgga acacggtaaa accatgaccg agaaggagat cgtggactat gtggccagcc
     3661 aggttacaac cgccaagaag ctgcgcggtg gtgttgtgtt cgtggacgag gtgcctaaag
     3721 gactgaccgg caagttggac gcccgcaaga tccgcgagat tctcattaag gccaagaagg
     3781 gcggcaagat cgccgtgaat tctcacggct tccctcccga ggtggaggag caggccgccg
     3841 gcaccctgcc catgagctgc gcccaggaga gcggcatgga tagacaccct gctgcttgcg
     3901 ccagcgccag gatcaacgtc taaggccgcg actctagaac gcgtcactgc ggctcatatg
     3961 tttttactgc tgactacaga catcagcgcg taccggttag taatgatcga caatcaacct
     4021 ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg
     4081 ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc
     4141 attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt
     4201 gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc
     4261 attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg
     4321 gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact
     4381 gacaattccg tggtgttgtc ggggaagctg acgtcctttc catggctgct cgcctgtgtt
     4441 gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg
     4501 gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc
     4561 cctcagacga gtcggatctc cctttgggcc gcctccccgc ctggcgatgg taccggtgtg
     4621 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
     4681 caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
     4741 tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc
     4801 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg
     4861 aggccgcctc tgcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag
     4921 gcttttgcaa aaagctcccg ggagcttgta tatccatttt cggatctgat cagcacctag
     4981 catggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga
     5041 cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta
     5101 cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac
     5161 cctcgtgacc accttcacct acggcgtgca gtgcttcgcc cgctaccccg accacatgaa
     5221 gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt
     5281 cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct
     5341 ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca
     5401 caagctggag tacaactaca acagccacaa ggtctatatc accgccgaca agcagaagaa
     5461 cggcatcaag gtgaacttca agacccgcca caacatcgag gacggcagcg tgcagctcgc
     5521 cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca
     5581 ctacctgagc acccagtccg ccctgagcaa agaccccaac gagaagcgcg atcacatggt
     5641 cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaagta
     5701 aggtaccttt aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag
     5761 aaaagggggg actggaaggg ctaattcact cccaacgaag acaagatctg ctttttgctt
     5821 gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc taactaggga
     5881 acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc
     5941 tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct
     6001 ctagcagtag tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa
     6061 tatcagagag tgagaggaac ttgtttattg cagcttataa tggttacaaa taaagcaata
     6121 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
     6181 aactcatcaa tgtatcttat catgtctggc tctagctatc ccgcccctaa ctccgcccat
     6241 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
     6301 tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg
     6361 cttttttgga ggcctaggga cgtacccaat tcgccctata gtgagtcgta ttacgcgcgc
     6421 tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat
     6481 cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat
     6541 cgcccttccc aacagttgcg cagcctgaat ggcgaatggg acgcgccctg tagcggcgca
     6601 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta
     6661 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
     6721 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
     6781 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt
     6841 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga
     6901 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
     6961 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata
     7021 ttaacgctta caatttaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
     7081 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
     7141 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
     7201 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
     7261 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
     7321 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
     7381 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
     7441 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
     7501 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
     7561 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
     7621 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
     7681 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
     7741 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
     7801 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
     7861 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
     7921 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
     7981 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
     8041 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     8101 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     8161 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     8221 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     8281 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     8341 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     8401 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     8461 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     8521 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     8581 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     8641 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     8701 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     8761 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     8821 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     8881 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
     8941 gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc
     9001 gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg
     9061 agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta
     9121 tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca
     9181 gctatgacca tgattacgcc aagcgcgcaa ttaaccctca ctaaagggaa caaaagctgg
     9241 agctgcaagc tt
//
