LOCUS       sequence-370206-        5849 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  This is the Lazarou lab's standard retrovirus vector backbone for 
            expression of HA-tag fusions onto the N-terminus of cloned genes.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5849)
  AUTHORS   Nguyen TN, Sawa-Makarska J, Khuu G, Lam WK, Adriaenssens E, 
            Fracchiolla D, Shoebridge S, Bernklau D, Padman BS, Skulsuppaisarn 
            M, Lindblom RSJ, Martens S, Lazarou M
  TITLE     Unconventional initiation of PINK1/Parkin mitophagy by Optineurin.
  JOURNAL   Mol Cell. 2023 May 18;83(10):1693-1709.e9. doi: 
            10.1016/j.molcel.2023.04.021.
  PUBMED    37207627
REFERENCE   2  (bases 1 to 5849)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2023.04"; journalName: "Mol Cell"; date: 
            "2023-05-18- 18"; volume: "83"; issue: "10"; pages: "1693-1709.e9"
COMMENT     Sequence Label: pBMN HA
FEATURES             Location/Qualifiers
     source          1..5849
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             43..459
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     389..411
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     427..443
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     misc_feature    469..843
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     781..797
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     CDS             855..881
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     misc_feature    882..947
                     /label=MCS
                     /note="multiple cloning site"
     LTR             1135..1604
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(1734..1751)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1905..2493)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1985..2004)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2657..3448,3449..3517))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3280..3299
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3518..3622)
                     /gene="bla"
                     /label=AmpR promoter
     mobile_element  3767..4534
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
     LTR             4936..5405
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    5470..5827
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
ORIGIN
        1 tgtctgactg tgtttctgta tttgtctgaa aatatgggcc cgggccagac tgttaccact
       61 cccttaagtt tgaccttagg tcactggaaa gatgtcgagc ggatcgctca caaccagtcg
      121 gtagatgtca agaagagacg ttgggttacc ttctgctctg cagaatggcc aacctttaac
      181 gtcggatggc cgcgagacgg cacctttaac cgagacctca tcacccaggt taagatcaag
      241 gtcttttcac ctggcccgca tggacaccca gaccaggtcc cctacatcgt gacctgggaa
      301 gccttggctt ttgacccccc tccctgggtc aagccctttg tacaccctaa gcctccgcct
      361 cctcttcctc catccgcccc gtctctcccc cttgaacctc ctcgttcgac cccgcctcga
      421 tcctcccttt atccagccct cactccttct ctaggcgccc ccatatggcc atatgagatc
      481 ttatatgggg cacccccgcc ccttgtaaac ttccctgacc ctgacatgac aagagttact
      541 aacagcccct ctctccaagc tcacttacag gctctctact tagtccagca cgaagtctgg
      601 agacctctgg cggcagccta ccaagaacaa ctggaccgac cggtggtacc tcacccttac
      661 cgagtcggcg acacagtgtg ggtccgccga caccagacta agaacctaga acctcgctgg
      721 aaaggacctt acacagtcct gctgaccacc cccaccgccc tcaaagtaga cggcatcgca
      781 gcttggatac acgccgccca cgtgaaggct gccgaccccg ggggtggacc atcctctaga
      841 ctgccgccac catgtaccca tacgatgttc cagattacgc ttccggactc agatctcgag
      901 ctcaagcttc gaattctgca gtcgacggta ccgcgggccc gggatccacc ggatctagat
      961 aactgatcac gataaaataa aagattttat ttagtctcca gaaaaagggg ggaatgaaag
     1021 accccacctg taggtttggc aagctagctt aagtaacgcc attttgcaag gcatggaaaa
     1081 atacataact gagaatagag aagttcagat caaggtcagg aacagatgga acagctgaat
     1141 atgggccaaa caggatatct gtggtaagca gttcctgccc cggctcaggg ccaagaacag
     1201 atggaacagc tgaatatggg ccaaacagga tatctgtggt aagcagttcc tgccccggct
     1261 cagggccaag aacagatggt ccccagatgc ggtccagccc tcagcagttt ctagagaacc
     1321 atcagatgtt tccagggtgc cccaaggacc tgaaatgacc ctgtgcctta tttgaactaa
     1381 ccaatcagtt cgcttctcgc ttctgttcgc gcgcttctgc tccccgagct caataaaaga
     1441 gcccacaacc cctcactcgg ggcgccagtc ctccgattga ctgagtcgcc cgggtacccg
     1501 tgtatccaat aaaccctctt gcagttgcat ccgacttgtg gtctcgctgt tccttgggag
     1561 ggtctcctct gagtgattga ctacccgtca gcgggggtct ttcacatgca gcatgtatca
     1621 aaattaattt ggtttttttt cttaagtatt tacattaaat ggccatagtt gcattaatga
     1681 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
     1741 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
     1801 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     1861 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     1921 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     1981 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     2041 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     2101 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     2161 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     2221 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     2281 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     2341 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     2401 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     2461 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     2521 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     2581 aggatcttca cctagatcct tttgcggccg gccgcaaatc aatctaaagt atatatgagt
     2641 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     2701 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
     2761 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
     2821 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
     2881 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
     2941 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
     3001 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
     3061 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
     3121 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
     3181 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
     3241 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
     3301 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
     3361 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
     3421 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
     3481 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
     3541 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
     3601 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccaccagct ttgctcttag
     3661 gagtttccta atacatccca aactcaaata tataaagcat ttgacttgtt ctatgcccta
     3721 gggggcgggg ggaagctaag ccagcttttt ttaacattta aaatgtggta atgactccaa
     3781 cttattgata gtgttttatg ttcagataat gcccgatgac tttgtcatgc agctccaccg
     3841 attttgagaa cgacagcgac ttccgtccca gccgtgccag gtgctgcctc agattcaggt
     3901 tatgccgctc aattcgctgc gtatatcgct tgctgattac gtgcagcttt cccttcaggc
     3961 gggattcata cagcggccag ccatccgtca tccatatcac cacgtcaaag ggtgacagca
     4021 ggctcataag acgccccagc gtcgccatag tgcgttcacc gaatacgtgc gcaacaaccg
     4081 tcttccggag actgtcatac gcgtaaaaca gccagcgctg gcgcgattta gccccgacat
     4141 agccccactg ttcgtccatt tccgcgcaga cgatgacgtc actgcccggc tgtatgcgcg
     4201 aggttaccga ctgcggcctg agttttttaa gtgacgtaaa atcgtgttga ggccaacgcc
     4261 cataatgcgg gctgttgccc ggcatccaac gccattcatg gccatatcaa tgattttctg
     4321 gtgcgtaccg ggttgagaag cggtgtaagt gaactgcagt tgccatgttt tacggcagtg
     4381 agagcagaga tagcgctgat gtccggcggt gcttttgccg ttacgcacca ccccgtcagt
     4441 agctgaacag gagggacagc tgatagaaac agaagccact ggagcacctc aaaaacacca
     4501 tcatacacta aatcagtaag ttggcagcat caccttaaaa tgttaattcc attttaaatg
     4561 cacagatgtt tttatttcat aagggtttca atgtgcatga atgctgcaat attcctgtta
     4621 ccaaagctag tataaataaa aatagataaa cgtggaaatt acttagagtt tctgtcatta
     4681 acgtttcctt cctcagttga caacataaat gcgctgctga gaagccagtt tgcatctgtc
     4741 aggatcaatt tcccattatg ccagtcatat taattactag tcaattagtt gatttttatt
     4801 tttgacatat acatgtgaaa gaccccacct gtaggtttgg caagctagct taagtaacgc
     4861 cattttgcaa ggcatggaaa aatacataac tgagaataga aaagttcaga tcaaggtcag
     4921 gaacagatgg aacagctgaa tatgggccaa acaggatatc tgtggtaagc agttcctgcc
     4981 ccggctcagg gccaagaaca gatggaacag ctgaatatgg gccaaacagg atatctgtgg
     5041 taagcagttc ctgccccggc tcagggccaa gaacagatgg tccccagatg cggtccagcc
     5101 ctcagcagtt tctagagaac catcagatgt ttccagggtg ccccaaggac ctgaaatgac
     5161 cctgtgcctt atttgaacta accaatcagt tcgcttctcg cttctgttcg cgcgcttctg
     5221 ctccccgagc tcaataaaag agcccacaac ccctcactcg gcgcgccagt cctccgattg
     5281 actgagtcgc ccgggtaccc gtgtatccaa taaaccctct tgcagttgca tccgacttgt
     5341 ggtctcgctg ttccttggga gggtctcctc tgagtgattg actacccgtc agcgggggtc
     5401 tttcatttgg gggctcgtcc gggatcggga gacccctgcc cagggaccac cgacccacca
     5461 ccgggaggta agctggccag caacttatct gtgtctgtcc gattgtctag tgtctatgac
     5521 tgattttatg cgcctgcgtc ggtactagtt agctaactag ctctgtatct ggcggacccg
     5581 tggtggaact gacgagttcg gaacacccgg ccgcaaccct gggagacgtc ccagggactt
     5641 cgggggccgt ttttgtggcc cgacctgagt ccaaaaatcc cgatcgtttt ggactctttg
     5701 gtgcaccccc cttagaggag ggatatgtgg ttctggtagg agacgagaac ctaaaacagt
     5761 tcccgcctcc gtctgaattt ttgctttcgg tttgggaccg aagccgcgcc gcgcgtcttg
     5821 tctgctgcag catcgttctg tgttgtctc
//
