LOCUS       sequence-370208-        7370 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For expression of human ATG13(W50D).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7370)
  AUTHORS   Ren X, Nguyen TN, Lam WK, Buffalo CZ, Lazarou M, Yokom AL, Hurley JH
  TITLE     Structural basis for ATG9A recruitment to the ULK1 complex in 
            mitophagy initiation.
  JOURNAL   Sci Adv. 2023 Feb 15;9(7):eadg2997. doi: 10.1126/sciadv.adg2997. 
            Epub 2023 Feb 15.
  PUBMED    36791199
REFERENCE   2  (bases 1 to 7370)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2023-02-15"; volume: "9(7):eadg2997. doi"; pages: " 
            10.1126/sciadv.adg2997. Epub 2023 Feb 15"
COMMENT     Sequence Label: pBMN HA-ATG13(W50D)
FEATURES             Location/Qualifiers
     source          1..7370
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             266..735
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(865..882)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1036..1624)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1116..1135)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1788..2579,2580..2648))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2411..2430
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2649..2753)
                     /gene="bla"
                     /label=AmpR promoter
     mobile_element  2898..3665
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
     LTR             4067..4536
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    4601..4958
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             5023..5439
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     5369..5391
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     5407..5423
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     misc_feature    5449..5823
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     5761..5777
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     CDS             5835..5861
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 ttaccagaga agctggctgt gcatgagaag aatgtccgcg agtttgatgc ctttgtggaa
       61 accctgcagt aaggatccac cggatctaga taactgatca cgataaaata aaagatttta
      121 tttagtctcc agaaaaaggg gggaatgaaa gaccccacct gtaggtttgg caagctagct
      181 taagtaacgc cattttgcaa ggcatggaaa aatacataac tgagaataga gaagttcaga
      241 tcaaggtcag gaacagatgg aacagctgaa tatgggccaa acaggatatc tgtggtaagc
      301 agttcctgcc ccggctcagg gccaagaaca gatggaacag ctgaatatgg gccaaacagg
      361 atatctgtgg taagcagttc ctgccccggc tcagggccaa gaacagatgg tccccagatg
      421 cggtccagcc ctcagcagtt tctagagaac catcagatgt ttccagggtg ccccaaggac
      481 ctgaaatgac cctgtgcctt atttgaacta accaatcagt tcgcttctcg cttctgttcg
      541 cgcgcttctg ctccccgagc tcaataaaag agcccacaac ccctcactcg gggcgccagt
      601 cctccgattg actgagtcgc ccgggtaccc gtgtatccaa taaaccctct tgcagttgca
      661 tccgacttgt ggtctcgctg ttccttggga gggtctcctc tgagtgattg actacccgtc
      721 agcgggggtc tttcacatgc agcatgtatc aaaattaatt tggttttttt tcttaagtat
      781 ttacattaaa tggccatagt tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
      841 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
      901 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
      961 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     1021 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     1081 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     1141 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     1201 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     1261 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     1321 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     1381 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     1441 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
     1501 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     1561 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     1621 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     1681 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttgcggcc
     1741 ggccgcaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
     1801 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
     1861 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
     1921 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
     1981 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
     2041 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
     2101 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
     2161 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
     2221 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
     2281 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
     2341 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
     2401 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
     2461 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
     2521 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
     2581 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
     2641 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
     2701 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
     2761 ccccgaaaag tgccaccagc tttgctctta ggagtttcct aatacatccc aaactcaaat
     2821 atataaagca tttgacttgt tctatgccct agggggcggg gggaagctaa gccagctttt
     2881 tttaacattt aaaatgtggt aatgactcca acttattgat agtgttttat gttcagataa
     2941 tgcccgatga ctttgtcatg cagctccacc gattttgaga acgacagcga cttccgtccc
     3001 agccgtgcca ggtgctgcct cagattcagg ttatgccgct caattcgctg cgtatatcgc
     3061 ttgctgatta cgtgcagctt tcccttcagg cgggattcat acagcggcca gccatccgtc
     3121 atccatatca ccacgtcaaa gggtgacagc aggctcataa gacgccccag cgtcgccata
     3181 gtgcgttcac cgaatacgtg cgcaacaacc gtcttccgga gactgtcata cgcgtaaaac
     3241 agccagcgct ggcgcgattt agccccgaca tagccccact gttcgtccat ttccgcgcag
     3301 acgatgacgt cactgcccgg ctgtatgcgc gaggttaccg actgcggcct gagtttttta
     3361 agtgacgtaa aatcgtgttg aggccaacgc ccataatgcg ggctgttgcc cggcatccaa
     3421 cgccattcat ggccatatca atgattttct ggtgcgtacc gggttgagaa gcggtgtaag
     3481 tgaactgcag ttgccatgtt ttacggcagt gagagcagag atagcgctga tgtccggcgg
     3541 tgcttttgcc gttacgcacc accccgtcag tagctgaaca ggagggacag ctgatagaaa
     3601 cagaagccac tggagcacct caaaaacacc atcatacact aaatcagtaa gttggcagca
     3661 tcaccttaaa atgttaattc cattttaaat gcacagatgt ttttatttca taagggtttc
     3721 aatgtgcatg aatgctgcaa tattcctgtt accaaagcta gtataaataa aaatagataa
     3781 acgtggaaat tacttagagt ttctgtcatt aacgtttcct tcctcagttg acaacataaa
     3841 tgcgctgctg agaagccagt ttgcatctgt caggatcaat ttcccattat gccagtcata
     3901 ttaattacta gtcaattagt tgatttttat ttttgacata tacatgtgaa agaccccacc
     3961 tgtaggtttg gcaagctagc ttaagtaacg ccattttgca aggcatggaa aaatacataa
     4021 ctgagaatag aaaagttcag atcaaggtca ggaacagatg gaacagctga atatgggcca
     4081 aacaggatat ctgtggtaag cagttcctgc cccggctcag ggccaagaac agatggaaca
     4141 gctgaatatg ggccaaacag gatatctgtg gtaagcagtt cctgccccgg ctcagggcca
     4201 agaacagatg gtccccagat gcggtccagc cctcagcagt ttctagagaa ccatcagatg
     4261 tttccagggt gccccaagga cctgaaatga ccctgtgcct tatttgaact aaccaatcag
     4321 ttcgcttctc gcttctgttc gcgcgcttct gctccccgag ctcaataaaa gagcccacaa
     4381 cccctcactc ggcgcgccag tcctccgatt gactgagtcg cccgggtacc cgtgtatcca
     4441 ataaaccctc ttgcagttgc atccgacttg tggtctcgct gttccttggg agggtctcct
     4501 ctgagtgatt gactacccgt cagcgggggt ctttcatttg ggggctcgtc cgggatcggg
     4561 agacccctgc ccagggacca ccgacccacc accgggaggt aagctggcca gcaacttatc
     4621 tgtgtctgtc cgattgtcta gtgtctatga ctgattttat gcgcctgcgt cggtactagt
     4681 tagctaacta gctctgtatc tggcggaccc gtggtggaac tgacgagttc ggaacacccg
     4741 gccgcaaccc tgggagacgt cccagggact tcgggggccg tttttgtggc ccgacctgag
     4801 tccaaaaatc ccgatcgttt tggactcttt ggtgcacccc ccttagagga gggatatgtg
     4861 gttctggtag gagacgagaa cctaaaacag ttcccgcctc cgtctgaatt tttgctttcg
     4921 gtttgggacc gaagccgcgc cgcgcgtctt gtctgctgca gcatcgttct gtgttgtctc
     4981 tgtctgactg tgtttctgta tttgtctgaa aatatgggcc cgggccagac tgttaccact
     5041 cccttaagtt tgaccttagg tcactggaaa gatgtcgagc ggatcgctca caaccagtcg
     5101 gtagatgtca agaagagacg ttgggttacc ttctgctctg cagaatggcc aacctttaac
     5161 gtcggayggc cgcgagacgg cacctttaac cgagacctca tcacccaggt taagatcaag
     5221 gtcttttcac ctggcccgca tggacaccca gaccaggtcc cctacatcgt gacctgggaa
     5281 gccttggctt ttgacccccc tccctgggtc aagccctttg tacaccctaa gcctccgcct
     5341 cctcttcctc catccgcccc gtctctcccc cttgaacctc ctcgttcgac cccgcctcga
     5401 tcctcccttt atccagccct cactccttct ctaggcgccc ccatatggcc atatgagatc
     5461 ttatatgggg cacccccgcc ccttgtaaac ttccctgacc ctgacatgac aagagttact
     5521 aacagcccct ctctccaagc tcacttacag gctctctact tagtccagca cgaagtctgg
     5581 agacctctgg cggcagccta ccaagaacaa ctggaccgac cggtggtacc tcacccttac
     5641 cgagtcggcg acacagtgtg ggtccgccga caccagacta agaacctaga acctcgctgg
     5701 aaaggacctt acacagtcct gctgaccacc cccaccgccc tcaaagtaga cggcatcgca
     5761 gcttggatac acgccgccca cgtgaaggct gccgaccccg ggggtggacc atcctctaga
     5821 ctgccgccac catgtaccca tacgatgttc cagattacgc ttccggactc agatctcgag
     5881 ctcaagcttc ggaaactgat ctcaattccc aggacagaaa ggacctggac aagtttatta
     5941 aattttttgc cctcaagact gtccaagtga ttgtccaggc tcggcttggt gaaaagattt
     6001 gcactcgttc atcatcttct ccaacgggtt cagatgactt caacttagca atcaaagaca
     6061 tcccagaggt tacacatgaa gcaaagaagg cactggcagg acagctgcct gcagtcggga
     6121 ggtccatgtg tgtggagatt tcacttaaga cttctgaggg agattccatg gagctggaaa
     6181 tatggtgtct tgaaatgaat gaaaagtgtg ataaagaaat caaagtttcc tacacggtgt
     6241 acaacagact gtcattgctg ctgaagtccc ttcttgctat aactagggtg acaccagcct
     6301 ataggctctc caggaaacaa gggcatgaat atgtcatatt atacaggata tattttggag
     6361 aagttcagct gagtggctta ggagaaggct tccagacagt tcgtgttggg acagtgggca
     6421 cccctgtggg caccatcact ctttcttgtg cttacagaat taacttggca ttcatgtcta
     6481 ccaggcaatt tgagaggacc ccacctatca tggggattat tattgatcac tttgtggacc
     6541 gtccctatcc cagctcctct cccatgcacc cctgcaatta cagaactgct ggtgaggaca
     6601 ctggagtaat atacccgtct gtagaagact ctcaagaagt gtgtaccacc tctttttcca
     6661 cctccccacc atcccagctc tcaagctctc gcctttccta tcagcctgct gccctgggcg
     6721 ttggatcagc tgacctggct tatccagtag tgtttgctgc tggcttaaat gctacacacc
     6781 ctcaccagct gatggttcct gggaaggaag gtggggtacc ccttgctccc aaccagcctg
     6841 tccatggtac ccaggctgac caggagagac tggcaacctg caccccttct gacagaaccc
     6901 actgtgctgc cacaccctcc agtagtgagg atactgaaac cgtatcaaac agcagtgagg
     6961 gacgggcctc ccctcacgat gtcttggaga ccatctttgt ccgaaaagtg ggggcttttg
     7021 tcaacaaacc cattaaccag gtgaccctga cgagtttgga tatacccttt gccatgtttg
     7081 ctcccaagaa tttggagctg gaggataccg atccaatggt gaatcctcca gattccccag
     7141 agactgaatc tcctctccag ggcagcctgc actcagatgg ctccagcggg ggcagcagtg
     7201 gcaataccca tgatgacttt gttatgatag actttaaacc agctttttct aaagatgaca
     7261 ttcttccgat ggacctgggg accttctatc gggagtttca gaacccacct cagctgagca
     7321 gcctctccat agatattgga gcacagtcca tggctgaaga cttggactca
//
