LOCUS       sequence-370209-        3111 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Zea Mays Nuclear Auxin Signaling Repressor BIF1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3111)
  AUTHORS   Ramos Baez R, Buckley Y, Yu H, Chen Z, Gallavotti A, Nemhauser JL, 
            Moss BL
  TITLE     A Synthetic Approach Allows Rapid Characterization of the Maize 
            Nuclear Auxin Response Circuit.
  JOURNAL   Plant Physiol. 2020 Apr;182(4):1713-1722. doi: 10.1104/pp.19.01475. 
            Epub 2020 Mar 2.
  PUBMED    32123041
REFERENCE   2  (bases 1 to 3111)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1104/pp.19.01475"; journalName: "Plant Physiol"; date: 
            "2020-04"; volume: "182"; issue: "4"; pages: "1713-1722"
COMMENT     Sequence Label: pENTR-BIF1
FEATURES             Location/Qualifiers
     source          1..3111
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      309..897
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     798..817
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1051..1068
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(1227..1254)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(1346..1432)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     1481..1503
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1495..1512
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1496..1512
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1528..1627
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2195..2294)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2311..2330)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2312..2330)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2335..2351)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2335..2351)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2453..2472)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             join(2464..3111,1..162)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2531..2550)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 cgagtcggaa tcgcagaccg ataccaggat cttgccatcc tatggaactg cctcggtgag
       61 ttttctcctt cattacagaa acggcttttt caaaaatatg gtattgataa tcctgatatg
      121 aataaattgc agtttcattt gatgctcgat gagtttttct aatcagaatt ggttaattgg
      181 ttgtaacact ggcagagcat tacgctgact tgacgggacg gcgcaagctc atgaccaaaa
      241 tcccttaacg tgagttacgc gtcgttccac tgagcgtcag accccgtaga aaagatcaaa
      301 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
      361 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
      421 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc
      481 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
      541 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
      601 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
      661 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
      721 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
      781 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
      841 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
      901 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
      961 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat
     1021 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag
     1081 cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac
     1141 gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatac gcgtaccgct
     1201 agccaggaag agtttgtaga aacgcaaaaa ggccatccgt caggatggcc ttctgcttag
     1261 tttgatgcct ggcagtttat ggcgggcgtc ctgcccgcca ccctccgggc cgttgcttca
     1321 caacgttcaa atccgctccc ggcggatttg tcctactcag gagagcgttc accgacaaac
     1381 aacagataaa acgaaaggcc cagtcttccg actgagcctt tcgttttatt tgatgcctgg
     1441 cagttcccta ctctcgcgtt aacgctagca tggatgtttt cccagtcacg acgttgtaaa
     1501 acgacggcca gtcttaagct cgggccccaa ataatgattt tattttgact gatagtgacc
     1561 tgttcgttgc aacaaattga tgagcaatgc ttttttataa tgccaacttt gtacaaaaaa
     1621 gcaggcttca aaatgtcggt ggatacggag cggagctcca ccgagtcgtc cgcggcctcc
     1681 gggctcggct acgaggacac cgcgctcgcc ctcaccctcc gcctcccggg ctccgacccc
     1741 ggccgttcct ccccgctcgc cgcgccctcc gacgccgcgc cgtctcccaa gacgcgggtg
     1801 gtggggtggc cgccggtgag gtcgtaccgc aagaacgcgc tcgccgactc cagcaaggcc
     1861 aaccggtcag ccagtttcgt gaaggtggcc gtcgacggcg cggcctacct gcggaaggtg
     1921 gacctccagg cgtacggcgg ctacgaccag ctcctccgcg cgctccagga caagttcttc
     1981 tcccacttca ccatcaggaa gttcgccgac gacgagagga agctggtgga cgcggtgaac
     2041 gggacggagt acgtgcccac gtacgaggac aaggatggcg actggatgct cgtcggcgac
     2101 gtcccctgga agatgttcgt ggagacctgc cggcgccttc gcctgatgaa aggttcagag
     2161 gccgttaact tggcaccaag agccgcccga tgacacccag ctttcttgta caaagttggc
     2221 attataagaa agcattgctt atcaatttgt tgcaacgaac aggtcactat cagtcaaaat
     2281 aaaatcatta tttgccatcc agctgatatc ccctatagtg agtcgtatta catggtcata
     2341 gctgtttcct ggcagctctg gcccgtgtct caaaatctct gatgttacat tgcacaagat
     2401 aaaaatatat catcatgaac aataaaactg tctgcttaca taaacagtaa tacaaggggt
     2461 gttatgagcc atattcaacg ggaaacgtcg aggccgcgat taaattccaa catggatgct
     2521 gatttatatg ggtataaatg ggctcgcgat aatgtcgggc aatcaggtgc gacaatctat
     2581 cgcttgtatg ggaagcccga tgcgccagag ttgtttctga aacatggcaa aggtagcgtt
     2641 gccaatgatg ttacagatga gatggtcaga ctaaactggc tgacggaatt tatgcctctt
     2701 ccgaccatca agcattttat ccgtactcct gatgatgcat ggttactcac cactgcgatc
     2761 cccggaaaaa cagcattcca ggtattagaa gaatatcctg attcaggtga aaatattgtt
     2821 gatgcgctgg cagtgttcct gcgccggttg cattcgattc ctgtttgtaa ttgtcctttt
     2881 aacagcgatc gcgtatttcg tctcgctcag gcgcaatcac gaatgaataa cggtttggtt
     2941 gatgcgagtg attttgatga cgagcgtaat ggctggcctg ttgaacaagt ctggaaagaa
     3001 atgcataaac ttttgccatt ctcaccggat tcagtcgtca ctcatggtga tttctcactt
     3061 gataacctta tttttgacga ggggaaatta ataggttgta ttgatgttgg a
//
