LOCUS       sequence-37021-e         588 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 588)
  AUTHORS   Zhu C, Smith T, McNulty J, Rayla AL, Lakshmanan A, Siekmann AF, 
            Buffardi M, Meng X, Shin J, Padmanabhan A, Cifuentes D, Giraldez AJ,
            Look AT, Epstein JA, Lawson ND, Wolfe SA
  TITLE     Evaluation and application of modularly assembled zinc-finger 
            nucleases in zebrafish.
  JOURNAL   Development. 2011 Oct;138(20):4555-64.
  PUBMED    21937602
REFERENCE   2  (bases 1 to 588)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Development."; date: "2011-10"; volume: "138(20)"; pages: "4555-64"
COMMENT     Sequence Label: pBS-F3-GCC
FEATURES             Location/Qualifiers
     source          1..588
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     15..31
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     15..31
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     50..70
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        52..70
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(390..409)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(391..409)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(419..436)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(419..435)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(428..450)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 cggatacatt tccacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc
       61 tcactaaagg gaacaaaagc tggagctcca ccgcggtggc ggccgctcta gattatttct
      121 cacccgtgtg gatcttggtg tgacgggtca ggtcagaacg gtccgcgaac ttcctgccgc
      181 agatgtcgca tgcaaaaggc ttctcgcctg tgtgggtgcg gatgtgggtg gtaaggtggt
      241 cactacgact gaagttacgc atacagattc gacactggaa gggcttctga ccggtatgga
      301 tgcggatatg gcgggtaagc tcatccgagc gagaaaagcg gcgatcgcag gactcgacag
      361 ggcaagcata tgggcgggta cccaattcgc cctatagtga gtcgtattac gcgcgctcac
      421 tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc
      481 ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc
      541 cttcccaaca gttgcgcagc ctgaatggcg aatgggacgc gccctgta
//
