LOCUS       sequence-370219-        3624 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Zea Mays Nuclear Auxin Signaling Repressor IAA9.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3624)
  AUTHORS   Ramos Baez R, Buckley Y, Yu H, Chen Z, Gallavotti A, Nemhauser JL, 
            Moss BL
  TITLE     A Synthetic Approach Allows Rapid Characterization of the Maize 
            Nuclear Auxin Response Circuit.
  JOURNAL   Plant Physiol. 2020 Apr;182(4):1713-1722. doi: 10.1104/pp.19.01475. 
            Epub 2020 Mar 2.
  PUBMED    32123041
REFERENCE   2  (bases 1 to 3624)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1104/pp.19.01475"; journalName: "Plant Physiol"; date: 
            "2020-04"; volume: "182"; issue: "4"; pages: "1713-1722"
COMMENT     Sequence Label: pENTR-ZmIAA9
FEATURES             Location/Qualifiers
     source          1..3624
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     342..361
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     595..612
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(771..798)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(890..976)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     1025..1047
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1039..1056
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1040..1056
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1072..1171
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2252..2351)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2368..2387)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2369..2387)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2392..2408)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2392..2408)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2510..2529)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2521..3330
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2588..2607)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      join(3477..3624,1..441)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
       61 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
      121 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
      181 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
      241 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
      301 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
      361 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
      421 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
      481 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
      541 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag
      601 tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg
      661 ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc
      721 aacgcaatta atacgcgtac cgctagccag gaagagtttg tagaaacgca aaaaggccat
      781 ccgtcaggat ggccttctgc ttagtttgat gcctggcagt ttatggcggg cgtcctgccc
      841 gccaccctcc gggccgttgc ttcacaacgt tcaaatccgc tcccggcgga tttgtcctac
      901 tcaggagagc gttcaccgac aaacaacaga taaaacgaaa ggcccagtct tccgactgag
      961 cctttcgttt tatttgatgc ctggcagttc cctactctcg cgttaacgct agcatggatg
     1021 ttttcccagt cacgacgttg taaaacgacg gccagtctta agctcgggcc ccaaataatg
     1081 attttatttt gactgatagt gacctgttcg ttgcaacaaa ttgatgagca atgctttttt
     1141 ataatgccaa ctttgtacaa aaaagcaggc ttcaaaatgg gagactccgg aggaagaaga
     1201 ggaccacctc ttcctcggct gctagatctc atcccggacg ggaaagaacg gaaggggagg
     1261 gaagcgcaag acgcgggaag gtcgaagaac agcggcggct tcgggggcga ggaggacgcg
     1321 aagctggagc tcaagctcgg gcctccgggt ctcactctcg tagaagaagg aacgacagct
     1381 accgtgccaa gagatggagg cgtactacag cgagagacga cgaccacccc gaccctctcg
     1441 ctcgggtgct tcccacgtaa cccctccaag cctgcggtag acgcagctac agctgggacg
     1501 aagagggggt tcttcgacac cgccgtcgag gccaagacag aaggccgcga cgagcggatg
     1561 gagcagcagg ccggagctgg atgtgggaac gagctagcac tggacgagaa gacggcagcc
     1621 gcggccgcga gtgagagaca gaaagggtcc tgctgcccgc cgacgccaca gcacgcccct
     1681 cctgctgcga ccgtgcacag cggagcacac gtcctgcagc tgggaagaag gccttcggct
     1741 ccggttgtcg gttggccccc ggtcagatcc ttcaggagaa accttgctca tcatcatcat
     1801 ggaagctcct ccaaacaacc caccgagcca cagaacagcg aagctagcag gaaggagaag
     1861 cctgcctgca agaagaaccc tctcgtaaaa atcaacatgg acgggatccc catcggaagg
     1921 aaagtagacc ttgcagccta cgacagctac gagaggctat cactgggcgt caaagagctt
     1981 ttccacggtt ttcttcaagc tcaaaaggat atgtctccaa ctgctggaaa gatattctct
     2041 caattgttgg atggatctgg cgaatatacc ctggtctacg aagacaacga aggagacagg
     2101 atgctggtag gggacgtgcc gtggaatgtg tttgtgtcaa ctgctaaaag gctgagggtt
     2161 ttgaggagct cggagcttgc caaaggtttg gtaggcacaa ggccacctca gattgcggta
     2221 gctccgagaa gaggaccacc agattgctga cacccagctt tcttgtacaa agttggcatt
     2281 ataagaaagc attgcttatc aatttgttgc aacgaacagg tcactatcag tcaaaataaa
     2341 atcattattt gccatccagc tgatatcccc tatagtgagt cgtattacat ggtcatagct
     2401 gtttcctggc agctctggcc cgtgtctcaa aatctctgat gttacattgc acaagataaa
     2461 aatatatcat catgaacaat aaaactgtct gcttacataa acagtaatac aaggggtgtt
     2521 atgagccata ttcaacggga aacgtcgagg ccgcgattaa attccaacat ggatgctgat
     2581 ttatatgggt ataaatgggc tcgcgataat gtcgggcaat caggtgcgac aatctatcgc
     2641 ttgtatggga agcccgatgc gccagagttg tttctgaaac atggcaaagg tagcgttgcc
     2701 aatgatgtta cagatgagat ggtcagacta aactggctga cggaatttat gcctcttccg
     2761 accatcaagc attttatccg tactcctgat gatgcatggt tactcaccac tgcgatcccc
     2821 ggaaaaacag cattccaggt attagaagaa tatcctgatt caggtgaaaa tattgttgat
     2881 gcgctggcag tgttcctgcg ccggttgcat tcgattcctg tttgtaattg tccttttaac
     2941 agcgatcgcg tatttcgtct cgctcaggcg caatcacgaa tgaataacgg tttggttgat
     3001 gcgagtgatt ttgatgacga gcgtaatggc tggcctgttg aacaagtctg gaaagaaatg
     3061 cataaacttt tgccattctc accggattca gtcgtcactc atggtgattt ctcacttgat
     3121 aaccttattt ttgacgaggg gaaattaata ggttgtattg atgttggacg agtcggaatc
     3181 gcagaccgat accaggatct tgccatccta tggaactgcc tcggtgagtt ttctccttca
     3241 ttacagaaac ggctttttca aaaatatggt attgataatc ctgatatgaa taaattgcag
     3301 tttcatttga tgctcgatga gtttttctaa tcagaattgg ttaattggtt gtaacactgg
     3361 cagagcatta cgctgacttg acgggacggc gcaagctcat gaccaaaatc ccttaacgtg
     3421 agttacgcgt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     3481 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     3541 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     3601 agagcgcaga taccaaatac tgtt
//
