LOCUS       sequence-37022-e         900 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 900)
  AUTHORS   Senkel S, Waldner C, Ryffel GU, Thomas H
  TITLE     Improved conditional expression systems resulting in physiological 
            level of HNF4alpha expression confirm HNF4alpha induced apoptosis in
            the pancreatic beta-cell line INS-1.
  JOURNAL   BMC Res Notes. 2009 Oct 17;2:210. doi: 10.1186/1756-0500-2-210.
  PUBMED    19835622
REFERENCE   2  (bases 1 to 900)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Res 
            Notes."; date: "2009-10-17"; volume: "2:210. doi"; pages: " 
            10.1186/1756-0500-2-210"
COMMENT     Sequence Label: CMV-138_HNF4A2
FEATURES             Location/Qualifiers
     source          1..900
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    21..39
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    42..60
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     70..94
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             260..289
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             299..328
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             338..367
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             377..406
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             416..445
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             479..508
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 cngngggcnt tagcgagctc tccctatcag tgatagagat ctccctatca gtgatagaga
       61 tcgtcgacga gctcgtttag tgaaccgtca gatcgcctgg agacgccatc cacgctgttt
      121 tgacctccat agaagacacc gggaccgatc cagcctccgg actctagcgt ttaaacttaa
      181 gcttggtacc gagctcggat ccactagtcc agtgtggtgg aattctgcag atatccagca
      241 cagtggcggc cgcgctatgg agcaaaagct catttctgaa gaggacttga atgaaatgga
      301 gcaaaagctc atttctgaag aggacttgaa tgaaatggag caaaagctca tttctgaaga
      361 ggacttgaat gaaatggagc aaaagctcat ttctgaagag gacttgaatg aaatggagca
      421 aaagctcatt tctgaagagg acttgaatga aatggagagc ttgggcgacc tcaccatgga
      481 gcaaaagctc atttctgaag aggacttgaa ttcaaggcct ggtaccatgc gactctccaa
      541 aaccctcgtc gacatggaca tggccgacta cagtgctgca ctggacccag cctacaccac
      601 cctggaattt gagaatgtgc aggtgttgac gatgggcaat gacacgtccc catcagaagg
      661 caccaacctc aacgcgccca acagcctggg tgtcagcgcc ctgtgtgcca tctgcgggga
      721 ccgggccacg ggcaaacact acggtgcctc gagctgtgac ggctgcaagg gcttcttccg
      781 gaggagcgtg cggaagaacc acatgtactc ctgcagattt agccggcagt gcgtggtgga
      841 caaagacaag aggaaccagt gccgctactg caggctcaag aaatgcttcc gggctggcat
//
