LOCUS       sequence-370226-        3369 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Zea Mays Nuclear Auxin Signaling Repressor IAA29 with yeast codon 
            optimization.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3369)
  AUTHORS   Ramos Baez R, Buckley Y, Yu H, Chen Z, Gallavotti A, Nemhauser JL, 
            Moss BL
  TITLE     A Synthetic Approach Allows Rapid Characterization of the Maize 
            Nuclear Auxin Response Circuit.
  JOURNAL   Plant Physiol. 2020 Apr;182(4):1713-1722. doi: 10.1104/pp.19.01475. 
            Epub 2020 Mar 2.
  PUBMED    32123041
REFERENCE   2  (bases 1 to 3369)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1104/pp.19.01475"; journalName: "Plant Physiol"; date: 
            "2020-04"; volume: "182"; issue: "4"; pages: "1713-1722"
COMMENT     Sequence Label: pENTR-ZmIAA29 (yeast codon optimized)
FEATURES             Location/Qualifiers
     source          1..3369
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      296..884
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     785..804
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1038..1055
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(1214..1241)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(1333..1419)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     1468..1490
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1482..1499
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1483..1499
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1515..1614
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2440..2539)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2556..2575)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2557..2575)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2580..2596)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2580..2596)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2698..2717)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             join(2709..3369,1..149)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2776..2795)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 cagaccgata ccaggatctt gccatcctat ggaactgcct cggtgagttt tctccttcat
       61 tacagaaacg gctttttcaa aaatatggta ttgataatcc tgatatgaat aaattgcagt
      121 ttcatttgat gctcgatgag tttttctaat cagaattggt taattggttg taacactggc
      181 agagcattac gctgacttga cgggacggcg caagctcatg accaaaatcc cttaacgtga
      241 gttacgcgtc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
      301 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
      361 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
      421 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
      481 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
      541 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
      601 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
      661 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
      721 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
      781 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
      841 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
      901 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
      961 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
     1021 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca
     1081 aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg
     1141 actggaaagc gggcagtgag cgcaacgcaa ttaatacgcg taccgctagc caggaagagt
     1201 ttgtagaaac gcaaaaaggc catccgtcag gatggccttc tgcttagttt gatgcctggc
     1261 agtttatggc gggcgtcctg cccgccaccc tccgggccgt tgcttcacaa cgttcaaatc
     1321 cgctcccggc ggatttgtcc tactcaggag agcgttcacc gacaaacaac agataaaacg
     1381 aaaggcccag tcttccgact gagcctttcg ttttatttga tgcctggcag ttccctactc
     1441 tcgcgttaac gctagcatgg atgttttccc agtcacgacg ttgtaaaacg acggccagtc
     1501 ttaagctcgg gccccaaata atgattttat tttgactgat agtgacctgt tcgttgcaac
     1561 aaattgatga gcaatgcttt tttataatgc caactttgta caaaaaagca ggcttcaaaa
     1621 tgtctcctcc attggagcca cacgactaca taggtttgtc tgctgctgca gctgctgcac
     1681 caccaactcc aacttcttct tcttcttctt catcatctcc agctccaagg ttgacattga
     1741 ggttgggttt gcccggttct gaatctccag acagagatcg tgacagagat aggtgcgaag
     1801 acgttgctgc tgcattgtca ttgggtccat tgccagctac acctaaagct cccgctgctg
     1861 tctctgcaaa gagagctttt ccagacccag cacaaagacc cggtgctgct aaggcatctg
     1921 atgacaagca agcttcaccc gctgctccac cagctgctaa agctcaagtc gttggttggc
     1981 caccagtcag aaactacaga aaaaatactt tggctgcttc tgcttctaga tcaaaggctc
     2041 ccgctgctga agacgctgct tctgctgcta gaccaatgta cgtcaaggtc tctatggacg
     2101 gtgctccata cttgagaaaa gttgatatta agatgtattc atcttacgaa gatttgtctg
     2161 ttgctttgca aaaaatgttt tcttgtttca ttgctggtca atctggtttg cataaatcat
     2221 cttctaaaga tagattgact aacggttcta aagttgatgc tttgaaagat caagaatacg
     2281 tcttgacata cgaagacaag gacgctgact ggatgttggt cggtgacttg ccatgggact
     2341 atttcacttc tatttgtaga aaattgaaga ttatgagagg ttctgacgct gtcggtattg
     2401 ctccaaggac aattgagcag actggtcaaa ataaatgaca cccagctttc ttgtacaaag
     2461 ttggcattat aagaaagcat tgcttatcaa tttgttgcaa cgaacaggtc actatcagtc
     2521 aaaataaaat cattatttgc catccagctg atatccccta tagtgagtcg tattacatgg
     2581 tcatagctgt ttcctggcag ctctggcccg tgtctcaaaa tctctgatgt tacattgcac
     2641 aagataaaaa tatatcatca tgaacaataa aactgtctgc ttacataaac agtaatacaa
     2701 ggggtgttat gagccatatt caacgggaaa cgtcgaggcc gcgattaaat tccaacatgg
     2761 atgctgattt atatgggtat aaatgggctc gcgataatgt cgggcaatca ggtgcgacaa
     2821 tctatcgctt gtatgggaag cccgatgcgc cagagttgtt tctgaaacat ggcaaaggta
     2881 gcgttgccaa tgatgttaca gatgagatgg tcagactaaa ctggctgacg gaatttatgc
     2941 ctcttccgac catcaagcat tttatccgta ctcctgatga tgcatggtta ctcaccactg
     3001 cgatccccgg aaaaacagca ttccaggtat tagaagaata tcctgattca ggtgaaaata
     3061 ttgttgatgc gctggcagtg ttcctgcgcc ggttgcattc gattcctgtt tgtaattgtc
     3121 cttttaacag cgatcgcgta tttcgtctcg ctcaggcgca atcacgaatg aataacggtt
     3181 tggttgatgc gagtgatttt gatgacgagc gtaatggctg gcctgttgaa caagtctgga
     3241 aagaaatgca taaacttttg ccattctcac cggattcagt cgtcactcat ggtgatttct
     3301 cacttgataa ccttattttt gacgagggga aattaatagg ttgtattgat gttggacgag
     3361 tcggaatcg
//
