LOCUS       sequence-370227-        3360 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Zea Mays Nuclear Auxin Signaling Repressor IAA10.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3360)
  AUTHORS   Ramos Baez R, Buckley Y, Yu H, Chen Z, Gallavotti A, Nemhauser JL, 
            Moss BL
  TITLE     A Synthetic Approach Allows Rapid Characterization of the Maize 
            Nuclear Auxin Response Circuit.
  JOURNAL   Plant Physiol. 2020 Apr;182(4):1713-1722. doi: 10.1104/pp.19.01475. 
            Epub 2020 Mar 2.
  PUBMED    32123041
REFERENCE   2  (bases 1 to 3360)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1104/pp.19.01475"; journalName: "Plant Physiol"; date: 
            "2020-04"; volume: "182"; issue: "4"; pages: "1713-1722"
COMMENT     Sequence Label: pENTR-ZmIAA10
FEATURES             Location/Qualifiers
     source          1..3360
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(57..76)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      946..1534
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1435..1454
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1688..1705
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(1864..1891)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(1983..2069)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     2118..2140
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2132..2149
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2133..2149
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    2165..2264
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(3081..3180)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(3197..3216)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3198..3216)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3221..3237)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3221..3237)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3339..3358)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             join(3350..3360,1..799)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
ORIGIN
        1 tcaacgggaa acgtcgaggc cgcgattaaa ttccaacatg gatgctgatt tatatgggta
       61 taaatgggct cgcgataatg tcgggcaatc aggtgcgaca atctatcgct tgtatgggaa
      121 gcccgatgcg ccagagttgt ttctgaaaca tggcaaaggt agcgttgcca atgatgttac
      181 agatgagatg gtcagactaa actggctgac ggaatttatg cctcttccga ccatcaagca
      241 ttttatccgt actcctgatg atgcatggtt actcaccact gcgatccccg gaaaaacagc
      301 attccaggta ttagaagaat atcctgattc aggtgaaaat attgttgatg cgctggcagt
      361 gttcctgcgc cggttgcatt cgattcctgt ttgtaattgt ccttttaaca gcgatcgcgt
      421 atttcgtctc gctcaggcgc aatcacgaat gaataacggt ttggttgatg cgagtgattt
      481 tgatgacgag cgtaatggct ggcctgttga acaagtctgg aaagaaatgc ataaactttt
      541 gccattctca ccggattcag tcgtcactca tggtgatttc tcacttgata accttatttt
      601 tgacgagggg aaattaatag gttgtattga tgttggacga gtcggaatcg cagaccgata
      661 ccaggatctt gccatcctat ggaactgcct cggtgagttt tctccttcat tacagaaacg
      721 gctttttcaa aaatatggta ttgataatcc tgatatgaat aaattgcagt ttcatttgat
      781 gctcgatgag tttttctaat cagaattggt taattggttg taacactggc agagcattac
      841 gctgacttga cgggacggcg caagctcatg accaaaatcc cttaacgtga gttacgcgtc
      901 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
      961 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     1021 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     1081 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     1141 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     1201 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     1261 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     1321 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     1381 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     1441 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     1501 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     1561 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
     1621 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac
     1681 cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct
     1741 ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc
     1801 gggcagtgag cgcaacgcaa ttaatacgcg taccgctagc caggaagagt ttgtagaaac
     1861 gcaaaaaggc catccgtcag gatggccttc tgcttagttt gatgcctggc agtttatggc
     1921 gggcgtcctg cccgccaccc tccgggccgt tgcttcacaa cgttcaaatc cgctcccggc
     1981 ggatttgtcc tactcaggag agcgttcacc gacaaacaac agataaaacg aaaggcccag
     2041 tcttccgact gagcctttcg ttttatttga tgcctggcag ttccctactc tcgcgttaac
     2101 gctagcatgg atgttttccc agtcacgacg ttgtaaaacg acggccagtc ttaagctcgg
     2161 gccccaaata atgattttat tttgactgat agtgacctgt tcgttgcaac aaattgatga
     2221 gcaatgcttt tttataatgc caactttgta caaaaaagca ggcttcaaaa tgagcccgcc
     2281 actcgagcca catgactaca tcggcctgtc tgctgtggct gctgctgctc caccaacccc
     2341 aaccccaact agcagcagct ctagctctag cagcccagct ccaaggctga ctctgaggct
     2401 tggccttcca ggctctgagt ctccagacag ggatagggac tgctgcgagg atgtggctgc
     2461 tactctgtct ctgggcccac ttccagccgc tgctgctgtt tctgctaaga gggctttccc
     2521 agacccagct caaaggccag gcgctagcaa ggcttctgac gctaagcagc aggctagccc
     2581 agcagctcca ccagctgcta aggctcaagt tgttggctgg ccgccagtga ggaactaccg
     2641 caagaacact ctggccgctg ctaccgcctc taggtctaag gctccagctg aggaggctgc
     2701 ttctggcgct ggcccaatgt acgtgaaggt gagcatggac ggcgctccgt accttcgcaa
     2761 ggtggacatc aagatgtaca gcagctacga ggacctgagc ctggccctcg agaagatgtt
     2821 cagctgcttc attgctggcc agagcggcct gcacaagagc agctctaagg acaggctgac
     2881 caacggcagc aaggtggacg ctctgaagga ccaagagtac gtgctgacct acgaggacaa
     2941 ggacgccgac tggatgctgg tgggcgatct gccatgggac tacttcacca gcatctgccg
     3001 caagctgaag atcatgaggg gctctgacgc tgtgggcatt gccccaagga ctgttgagca
     3061 gaccggccag aacaagtgac acccagcttt cttgtacaaa gttggcatta taagaaagca
     3121 ttgcttatca atttgttgca acgaacaggt cactatcagt caaaataaaa tcattatttg
     3181 ccatccagct gatatcccct atagtgagtc gtattacatg gtcatagctg tttcctggca
     3241 gctctggccc gtgtctcaaa atctctgatg ttacattgca caagataaaa atatatcatc
     3301 atgaacaata aaactgtctg cttacataaa cagtaataca aggggtgtta tgagccatat
//
