LOCUS       sequence-370230-       10267 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Tet-On inducible expression of eGFP-iPAK4 in a lentiviral backbone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10267)
  AUTHORS   Lin D, Li X, Moult E, Park P, Tang B, Shen H, Grimm JB, Falco N, Jia
            BZ, Baker D, Lavis LD, Cohen AE
  TITLE     Time-tagged ticker tapes for intracellular recordings.
  JOURNAL   Nat Biotechnol. 2023 Jan 2. doi: 10.1038/s41587-022-01524-7.
  PUBMED    36593408
REFERENCE   2  (bases 1 to 10267)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2023 Jan 2. doi: 10.1038/s41587-022-01524-7."
COMMENT     Sequence Label: DL146_plenti_TRE_GFP-iPAK4
FEATURES             Location/Qualifiers
     source          1..10267
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(73..92)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        264..643
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        645..843
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             861..1041
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1088..1213
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1706..1939
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2124..2168
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2466..2583
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     primer_bind     2753..2775
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2767..2784
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2768..2784
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2794..2813
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2794..2812
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        2837..3151
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    2845..2863
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2881..2899
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2916..2934
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2952..2970
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2988..3006
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3023..3041
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3059..3077
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     3124..3148
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             3168..3881
                     /codon_start=1
                     /gene="mgfp5"
                     /product="GFP with folding enhancement mutations"
                     /label=mgfp5
                     /note="suitable for expression in plants due to removal of 
                     a cryptic intron"
                     /translation="MSKGEELFTGVVPILVELDGDVNGYKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKA
                     NFKTRHNIEDGGVQLADHYQQNTPIGDDPVLLPDNHYLSTQSALSKDPNEKRDHMVILE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(3198..3226)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     misc_feature    4985..5573
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5038..5058)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5456..5467)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5576..5592)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5577..5593)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             6098..6278
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6304..6321)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6310..6534
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6580..7008
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6667..6686)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6877..6898
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7017..7037)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7022..7351
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7202..7337
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7264..7283
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        7399..7446
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7465..7839
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    7969..8090
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8006..8025)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8060..8079
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8139..8155)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8139..8155)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8152..8174)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8163..8179
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8187..8193,8194..8211,8212..8217))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8232..8253
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8370..8387)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8541..9129)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8621..8640)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9300..10091,10092..10160))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9923..9942
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10161..10265)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 catttccccg aaaagtgcca cctgacgtcg acggatcggg agatctcccg atcccctatg
       61 gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtatc tgctccctgc
      121 ttgtgtgttg gaggtcgctg agtagtgcgc gagcaaaatt taagctacaa caaggcaagg
      181 cttgaccgac aattgcatga agaatctgct tagggttagg cgttttgcgc tgcttcgcga
      241 tgtacgggcc agatatacgc gttgacattg attattgact agttattaat agtaatcaat
      301 tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa
      361 tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt
      421 tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta
      481 aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt
      541 caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc
      601 tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca
      661 gtacatcaat gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat
      721 tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa
      781 caactccgcc ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag
      841 cagcgcgttt tgcctgtact gggtctctct ggttagacca gatctgagcc tgggagctct
      901 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag
      961 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt
     1021 cagtgtggaa aatctctagc agtggcgccc gaacagggac ttgaaagcga aagggaaacc
     1081 agaggagctc tctcgacgca ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg
     1141 gcggcgactg gtgagtacgc caaaaatttt gactagcgga ggctagaagg agagagatgg
     1201 gtgcgagagc gtcagtatta agcgggggag aattagatcg cgatgggaaa aaattcggtt
     1261 aaggccaggg ggaaagaaaa aatataaatt aaaacatata gtatgggcaa gcagggagct
     1321 agaacgattc gcagttaatc ctggcctgtt agaaacatca gaaggctgta gacaaatact
     1381 gggacagcta caaccatccc ttcagacagg atcagaagaa cttagatcat tatataatac
     1441 agtagcaacc ctctattgtg tgcatcaaag gatagagata aaagacacca aggaagcttt
     1501 agacaagata gaggaagagc aaaacaaaag taagaccacc gcacagcaag cggccgctga
     1561 tcttcagacc tggaggagga gatatgaggg acaattggag aagtgaatta tataaatata
     1621 aagtagtaaa aattgaacca ttaggagtag cacccaccaa ggcaaagaga agagtggtgc
     1681 agagagaaaa aagagcagtg ggaataggag ctttgttcct tgggttcttg ggagcagcag
     1741 gaagcactat gggcgcagcg tcaatgacgc tgacggtaca ggccagacaa ttattgtctg
     1801 gtatagtgca gcagcagaac aatttgctga gggctattga ggcgcaacag catctgttgc
     1861 aactcacagt ctggggcatc aagcagctcc aggcaagaat cctggctgtg gaaagatacc
     1921 taaaggatca acagctcctg gggatttggg gttgctctgg aaaactcatt tgcaccactg
     1981 ctgtgccttg gaatgctagt tggagtaata aatctctgga acagatttgg aatcacacga
     2041 cctggatgga gtgggacaga gaaattaaca attacacaag cttaatacac tccttaattg
     2101 aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt attggaatta gataaatggg
     2161 caagtttgtg gaattggttt aacataacaa attggctgtg gtatataaaa ttattcataa
     2221 tgatagtagg aggcttggta ggtttaagaa tagtttttgc tgtactttct atagtgaata
     2281 gagttaggca gggatattca ccattatcgt ttcagaccca cctcccaacc ccgaggggac
     2341 ccgacaggcc cgaaggaata gaagaagaag gtggagagag agacagagac agatccattc
     2401 gattagtgaa cggatcggca ctgcgtgcgc caattctgca gacaaatggc agtattcatc
     2461 cacaatttta aaagaaaagg ggggattggg gggtacagtg caggggaaag aatagtagac
     2521 ataatagcaa cagacataca aactaaagaa ttacaaaaac aaattacaaa aattcaaaat
     2581 tttcgggttt attacaggga cagcagagat ccagtttggt taattaacca ttcgccattc
     2641 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg
     2701 gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca
     2761 cgacgttgta aaacgacggc cagtgagcgc gcgtaatacg actcactata gggcgaattg
     2821 gctttcgtct tcactcgagt ttactcccta tcagtgatag agaacgtatg tcgagtttac
     2881 tccctatcag tgatagagaa cgatgtcgag tttactccct atcagtgata gagaacgtat
     2941 gtcgagttta ctccctatca gtgatagaga acgtatgtcg agtttactcc ctatcagtga
     3001 tagagaacgt atgtcgagtt tatccctatc agtgatagag aacgtatgtc gagtttactc
     3061 cctatcagtg atagagaacg tatgtcgagg taggcgtgta cggtgggagg cctatataag
     3121 cagagctcgt ttagtgaacc gtcagatcgc ctggaaccgg tgccaccatg agtaaaggag
     3181 aagaactttt cactggagtt gtcccaattc ttgttgaatt agatggtgat gttaatgggt
     3241 acaaattttc tgtcagtgga gagggtgaag gtgatgcaac atacggaaaa cttaccctta
     3301 aatttatttg cactactgga aaactacctg ttccatggcc aacacttgtc actactctca
     3361 cttatggtgt tcaatgcttt tcaagatatc cagatcatat gaagcggcac gacttcttca
     3421 agagcgccat gcctgaggga tacgtgcagg agaggaccat cttcttcaag gacgacggga
     3481 actacaagac acgtgctgaa gtcaagtttg agggagacac cctcgtcaac aggatcgagc
     3541 ttaagggaat cgatttcaag gaggacggaa acatcctcgg ccacaagttg gaatacaact
     3601 acaactccca caacgtatac atcatggccg acaagcaaaa gaacggcatc aaagccaact
     3661 tcaagacccg ccacaacatc gaagacggcg gcgtgcaact cgctgatcat tatcaacaaa
     3721 atactccaat tggcgatgac cctgtccttt taccagacaa ccattacctg tccacacaat
     3781 ctgccctttc gaaagatccc aacgaaaaga gagaccacat ggtcattctt gagtttgtaa
     3841 cggctgctgg gattacacat ggcatggatg aactatacaa atcaggtgga tccgaagcag
     3901 aggactggac agcagcccta ctgaacaggg gtcgcagtcg ccagcccctg gtactagggg
     3961 acaattgctt tgctgacttg gtgcacaact ggatggagct gcctgaggaa ttccccgccg
     4021 cccctgctgt tcctgggccc cctggccccc gctcaccaca gcgggagcca cagcgagtat
     4081 cccatgagca gttccgggct gccctgcagc tggtggtgga cccaggcgac ccccgctcct
     4141 acctggacaa cttcatcaag attggcgagg gctccacggg catcgtgtgc atcgccaccg
     4201 tgcgcagctc gggcaagctg gtggccgtca agaagatgga cctgcgcaag cagcagaggc
     4261 gcgagctgct cttcaacgag gtggtaatca tgagggacta ccagcacgag aatgtggtgg
     4321 agatgtacaa cagctacctg gtgggggacg agctctgggt ggtcatggag ttcctggaag
     4381 gaggcgccct caccgacatc gtcacccaca ccaggatgaa cgaggagcag atcgcagccg
     4441 tgtgccttgc agtgctgcag gccctgtcgg tgctccacgc ccagggcgtc atccaccggg
     4501 acatcaagag cgactcgatc ctgctgaccc atgatggcag ggtgaagctg tcagactttg
     4561 ggttctgcgc ccaggtgagc aaggaagtgc cccgaaggaa gtcgctggtc ggcacgccct
     4621 actggatggc cccagagctc atctcccgcc ttccctacgg gccagaggta gacatctggt
     4681 cgctggggat aatggtgatt gagatggtgg acggagagcc cccctacttc aacgagccac
     4741 ccctcaaagc catgaagatg attcgggaca acctgccacc ccgactgaag aacctgcaca
     4801 aggtgtcgcc atccctgaag ggcttcctgg accgcctgct ggtgcgagac cctgcccagc
     4861 gggccacggc agccgagctg ctgaagcacc cattcctggc caaggcaggg ccgcctgcca
     4921 gcatcgtgcc cctcatgcgc cagaaccgca ccagatgagc ggccgcgata tcaagcttat
     4981 cgataatcaa cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt
     5041 tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc
     5101 ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga
     5161 gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc
     5221 cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct
     5281 ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg
     5341 gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct
     5401 gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc
     5461 cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg
     5521 tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcatcgata
     5581 ccgtcgacct cgagacctag aaaaacatgg agcaatcaca agtagcaata cagcagctac
     5641 caatgctgat tgtgcctggc tagaagcaca agaggaggag gaggtgggtt ttccagtcac
     5701 acctcaggta cctttaagac caatgactta caaggcagct gtagatctta gccacttttt
     5761 aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagacaag atatccttga
     5821 tctgtggatc taccacacac aaggctactt ccctgattgg cagaactaca caccagggcc
     5881 agggatcaga tatccactga cctttggatg gtgctacaag ctagtaccag ttgagcaaga
     5941 gaaggtagaa gaagccaatg aaggagagaa cacccgcttg ttacaccctg tgagcctgca
     6001 tgggatggat gacccggaga gagaagtatt agagtggagg tttgacagcc gcctagcatt
     6061 tcatcacatg gcccgagagc tgcatccgga ctgtactggg tctctctggt tagaccagat
     6121 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
     6181 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
     6241 cctcagaccc ttttagtcag tgtggaaaat ctctagcagg gcccgtttaa acccgctgat
     6301 cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt
     6361 ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat
     6421 cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg
     6481 gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct atggcttctg
     6541 aggcggaaag aaccagctgg ggctctaggg ggtatcccca cgcgccctgt agcggcgcat
     6601 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag
     6661 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc
     6721 aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc
     6781 ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt
     6841 ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa
     6901 caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg
     6961 cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaattaa ttctgtggaa
     7021 tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
     7081 catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag
     7141 aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc
     7201 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt
     7261 ttttatttat gcagaggccg aggccgcctc tgcctctgag ctattccaga agtagtgagg
     7321 aggctttttt ggaggcctag gcttttgcaa aaagctcccg ggagcttgta tatccatttt
     7381 cggatctgat cagcacgtgt tgacaattaa tcatcggcat agtatatcgg catagtataa
     7441 tacgacaagg tgaggaacta aaccatggcc aagttgacca gtgccgttcc ggtgctcacc
     7501 gcgcgcgacg tcgccggagc ggtcgagttc tggaccgacc ggctcgggtt ctcccgggac
     7561 ttcgtggagg acgacttcgc cggtgtggtc cgggacgacg tgaccctgtt catcagcgcg
     7621 gtccaggacc aggtggtgcc ggacaacacc ctggcctggg tgtgggtgcg cggcctggac
     7681 gagctgtacg ccgagtggtc ggaggtcgtg tccacgaact tccgggacgc ctccgggccg
     7741 gccatgaccg agatcggcga gcagccgtgg gggcgggagt tcgccctgcg cgacccggcc
     7801 ggcaactgcg tgcacttcgt ggccgaggag caggactgac acgtgctacg agatttcgat
     7861 tccaccgccg ccttctatga aaggttgggc ttcggaatcg ttttccggga cgccggctgg
     7921 atgatcctcc agcgcgggga tctcatgctg gagttcttcg cccaccccaa cttgtttatt
     7981 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
     8041 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgt
     8101 ataccgtcga cctctagcta gagcttggcg taatcatggt catagctgtt tcctgtgtga
     8161 aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc
     8221 tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc
     8281 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc
     8341 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
     8401 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
     8461 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     8521 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     8581 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     8641 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     8701 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
     8761 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     8821 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     8881 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     8941 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc
     9001 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     9061 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     9121 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
     9181 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
     9241 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
     9301 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
     9361 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
     9421 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
     9481 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
     9541 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
     9601 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
     9661 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
     9721 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
     9781 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
     9841 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
     9901 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
     9961 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
    10021 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
    10081 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
    10141 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
    10201 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
    10261 ccgcgca
//
