LOCUS       sequence-370231-        3213 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Zea Mays Nuclear Auxin Signaling Repressor IAA5.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3213)
  AUTHORS   Ramos Baez R, Buckley Y, Yu H, Chen Z, Gallavotti A, Nemhauser JL, 
            Moss BL
  TITLE     A Synthetic Approach Allows Rapid Characterization of the Maize 
            Nuclear Auxin Response Circuit.
  JOURNAL   Plant Physiol. 2020 Apr;182(4):1713-1722. doi: 10.1104/pp.19.01475. 
            Epub 2020 Mar 2.
  PUBMED    32123041
REFERENCE   2  (bases 1 to 3213)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1104/pp.19.01475"; journalName: "Plant Physiol"; date: 
            "2020-04"; volume: "182"; issue: "4"; pages: "1713-1722"
COMMENT     Sequence Label: pENTR-ZmIAA5
FEATURES             Location/Qualifiers
     source          1..3213
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      815..1403
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1304..1323
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1557..1574
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(1733..1760)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(1852..1938)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     1987..2009
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2001..2018
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2002..2018
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    2034..2133
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2803..2902)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2919..2938)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2920..2938)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2943..2959)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2943..2959)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3061..3080)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             join(3072..3213,1..668)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(3139..3158)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 cagagttgtt tctgaaacat ggcaaaggta gcgttgccaa tgatgttaca gatgagatgg
       61 tcagactaaa ctggctgacg gaatttatgc ctcttccgac catcaagcat tttatccgta
      121 ctcctgatga tgcatggtta ctcaccactg cgatccccgg aaaaacagca ttccaggtat
      181 tagaagaata tcctgattca ggtgaaaata ttgttgatgc gctggcagtg ttcctgcgcc
      241 ggttgcattc gattcctgtt tgtaattgtc cttttaacag cgatcgcgta tttcgtctcg
      301 ctcaggcgca atcacgaatg aataacggtt tggttgatgc gagtgatttt gatgacgagc
      361 gtaatggctg gcctgttgaa caagtctgga aagaaatgca taaacttttg ccattctcac
      421 cggattcagt cgtcactcat ggtgatttct cacttgataa ccttattttt gacgagggga
      481 aattaatagg ttgtattgat gttggacgag tcggaatcgc agaccgatac caggatcttg
      541 ccatcctatg gaactgcctc ggtgagtttt ctccttcatt acagaaacgg ctttttcaaa
      601 aatatggtat tgataatcct gatatgaata aattgcagtt tcatttgatg ctcgatgagt
      661 ttttctaatc agaattggtt aattggttgt aacactggca gagcattacg ctgacttgac
      721 gggacggcgc aagctcatga ccaaaatccc ttaacgtgag ttacgcgtcg ttccactgag
      781 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
      841 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
      901 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
      961 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     1021 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     1081 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     1141 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     1201 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     1261 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     1321 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     1381 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     1441 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
     1501 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
     1561 agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt
     1621 ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc
     1681 gcaacgcaat taatacgcgt accgctagcc aggaagagtt tgtagaaacg caaaaaggcc
     1741 atccgtcagg atggccttct gcttagtttg atgcctggca gtttatggcg ggcgtcctgc
     1801 ccgccaccct ccgggccgtt gcttcacaac gttcaaatcc gctcccggcg gatttgtcct
     1861 actcaggaga gcgttcaccg acaaacaaca gataaaacga aaggcccagt cttccgactg
     1921 agcctttcgt tttatttgat gcctggcagt tccctactct cgcgttaacg ctagcatgga
     1981 tgttttccca gtcacgacgt tgtaaaacga cggccagtct taagctcggg ccccaaataa
     2041 tgattttatt ttgactgata gtgacctgtt cgttgcaaca aattgatgag caatgctttt
     2101 ttataatgcc aactttgtac aaaaaagcag gcttcaaaat gtcggtggag acggagcgga
     2161 gctccaccga gtcctccggg gcttccgggc tcgacttcga ggacaccgcg ctcacgctca
     2221 ccctccgcct cccgggctcc gcgccttccg ccgccgccgc cgccgcggct tccctgtccc
     2281 tctcctcctc gtcgtcctcc gccttccccg accccgaccg caagcgcgcc tcctccgacg
     2341 ctgaccccgg ccgctcctcc ccgctcgccg cgtcctccga cgctgcaccg gcacccaagg
     2401 ctcgtgtggt gggctggccg ccggtgaggt cgtaccgcaa gaacgcgctc gccgacgccg
     2461 cgggctccag caaggccgcc aagttcgtca aggtggccgt cgacggcgcg ccctacctgc
     2521 ggaaggtgga cctgcaggcg tacgccggct acgaccagct gctccgcgcg ctccaggaca
     2581 agttcttctc ccacttcacc atcaggaagt tcgccgacga cgagaggaag ctggtggacg
     2641 cggtgaacgg gacggagtac gtacccacgt acgaggacaa ggatggcgac tggatgctcg
     2701 tcggcgacgt cccctggaag atgttcgtgg aaacctgcca gcgccttcgt ctgatgaaag
     2761 gttcagaggc cgtgaacttg gcaccaagag ccgcccgatg acacccagct ttcttgtaca
     2821 aagttggcat tataagaaag cattgcttat caatttgttg caacgaacag gtcactatca
     2881 gtcaaaataa aatcattatt tgccatccag ctgatatccc ctatagtgag tcgtattaca
     2941 tggtcatagc tgtttcctgg cagctctggc ccgtgtctca aaatctctga tgttacattg
     3001 cacaagataa aaatatatca tcatgaacaa taaaactgtc tgcttacata aacagtaata
     3061 caaggggtgt tatgagccat attcaacggg aaacgtcgag gccgcgatta aattccaaca
     3121 tggatgctga tttatatggg tataaatggg ctcgcgataa tgtcgggcaa tcaggtgcga
     3181 caatctatcg cttgtatggg aagcccgatg cgc
//
