LOCUS       sequence-370233-       10560 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  GPCR/G protein BRET biosensor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10560)
  AUTHORS   Janicot R, Maziarz M, Park JC, Zhao J, Luebbers A, Green E, 
            Philibert CE, Zhang H, Layne MD, Wu JC, Garcia-Marcos M
  TITLE     Direct interrogation of context-dependent GPCR activity with a 
            universal biosensor platform.
  JOURNAL   Cell. 2024 Feb 16:S0092-8674(24)00065-5. doi: 
            10.1016/j.cell.2024.01.028.
  PUBMED    38412860
REFERENCE   2  (bases 1 to 10560)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2024 
            Feb 16:S0092-8674(24)00065-5. doi: 10.1016/j.cell.2024.01.028."
COMMENT     Sequence Label: pLVX-CMV-Galpha-13 ONE-GO
FEATURES             Location/Qualifiers
     source          1..10560
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..204
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     155..175
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     201..225
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     complement(1045..1064)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1392..1413
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    join(2230..2804,2805..2807,2808..2816)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(2397..2414)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     2624..2643
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             2827..2847
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="MGSSKSK"
     CDS             3496..4011
                     /codon_start=1
                     /product="NanoLuc(R) luciferase"
                     /label=Nluc
                     /note="human codon-optimized"
                     /translation="MVFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQR
                     IVLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGV
                     TPNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGW
                     RLCERILA"
     misc_feature    4025..4613
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4078..4098)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4496..4507)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4820..5453
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(5581..5597)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5581..5597)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5594..5616)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5605..5621
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5629..5635,5636..5653,5654..5659))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5674..5695
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5812..5829)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5983..6571)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6063..6082)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6742..7533,7534..7602))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7365..7384
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7603..7707)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    7755..7889
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7792..7811)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7846..7865
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             8057..8690
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    8737..8862
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9359..9592
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9777..9821
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    10084..10199
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     enhancer        10257..10560
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
       61 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
      121 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
      181 tgggaggtct atataagcag agctcgttta gtgaaccgtc agatcgcctg gagacgccat
      241 ccacgctgtt ttgacctcca tagaagacac cgactctact agaggatcta tttccggtga
      301 attcctcgag actagttcta gagcggccgc ggatccatgg cggacttcct gccgtcgcgg
      361 tccgtgctgt ccgtgtgctt ccccggctgc ctgctgacga gtggcgaggc cgagcagcaa
      421 cgcaagtcca aggagatcga caaatgcctg tctcgggaaa agacctatgt gaagcggctg
      481 gtgaagatcc tgctgctggg cgcgggcgag agcggcaagt ccaccttcct gaagcagatg
      541 cggatcatcc acgggcagga cttcgaccag cgcgcgcgcg aggagttccg ccccaccatc
      601 tacagcaacg tgatcaaagg tatgagggtg ctggttgatg ctcgagagaa gcttcatatt
      661 ccctggggag acaactcaaa ccaacaacat ggagataaga tgatgtcgtt tgatacccgg
      721 gccccgcggg gatcaggcat ggtttcaaag ggagaagagt tgttcacagg ggttgtaccg
      781 attcttgtag aacttgacgg agatgtcaac gggcacaagt ttagtgtaag tggagaaggc
      841 gagggcgatg cgacctatgg aaaactgacc cttaaattta tttgtactac gggtaaattg
      901 cccgttccct ggccgacatt ggtaactaca ttcggatatg gtttgatgtg ctttgcaagg
      961 tatccggatc acatgaagca gcatgatttc ttcaagtccg ccatgcccga aggctacgtc
     1021 caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag
     1081 ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac
     1141 ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg
     1201 gccgacaagc agaagaacgg catcaaggtg aacttcaaga tccgccacaa catcgaggac
     1261 ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg
     1321 ctgctgcccg acaaccacta cctgagctac cagtccgcca tgagcaaaga ccccaacgag
     1381 aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg
     1441 gacgagctgt acaagggatc aggcccgcgg atggcagccc aaggaatggt ggaaacaagg
     1501 gttttcttac aatatcttcc tgctataaga gcattatggg cagacagcgg catacagaat
     1561 gcctatgacc ggcgtcgaga atttcaactg ggtgaatctg taaaatattt cctggataac
     1621 ttggataaac ttggagaacc agagtatatg ccaacagaac aagatattct gcttgccaga
     1681 agacccacca aaggcatcca tgaatacgac tttgaaataa aaaatgttcc tttcaaaatg
     1741 gttgatgtag gtggtcagag atcagaaagg aaacgttggt ttgaatgttt cgacagtgtg
     1801 acatcaatac ttttccttgt ttcctcaagt gaatttgacc aggtgcttat ggaagatcga
     1861 ctgaccaatc gccttacaga gtctctgaac atttttgaaa caatcgtcaa taaccgggtt
     1921 ttcagcaatg tctccataat tctgttctta aacaagacag acttgcttga ggagaaggtg
     1981 caaattgtga gcatcaaaga ctatttccta gaatttgaag gggatcccca ctgcttaaga
     2041 gacgtccaaa aattcctggt ggaatgtttc cggaacaaac gccgggacca gcaacagaag
     2101 cccttatacc accacttcac cactgctatc aacacggaga acatccgcct tgttttccgt
     2161 gacgtgaagg atactattct gcatgacaac ctcaagcagc ttatgctaca gtgatctagg
     2221 cggccgccgc ccctctccct cccccccccc taacgttact ggccgaagcc gcttggaata
     2281 aggccggtgt gcgtttgtct atatgttatt ttccaccata ttgccgtctt ttggcaatgt
     2341 gagggcccgg aaacctggcc ctgtcttctt gacgagcatt cctaggggtc tttcccctct
     2401 cgccaaagga atgcaaggtc tgttgaatgt cgtgaaggaa gcagttcctc tggaagcttc
     2461 ttgaagacaa acaacgtctg tagcgaccct ttgcaggcag cggaaccccc cacctggcga
     2521 caggtgcctc tgcggccaaa agccacgtgt ataagataca cctgcaaagg cggcacaacc
     2581 ccagtgccac gttgtgagtt ggatagttgt ggaaagagtc aaatggctct cctcaagcgt
     2641 attcaacaag gggctgaagg atgcccagaa ggtaccccat tgtatgggat ctgatctggg
     2701 gcctcggtgc acatgcttta catgtgttta gtcgaggtta aaaaaacgtc taggcccccc
     2761 gaaccacggg gacgtggttt tcctttgaaa aacacgatga taagcttgcc acaacccgta
     2821 ccaaagatgg ggagtagcaa gagcaagacg tcgaattccg gaatcaagct tctgattatt
     2881 ggcccagagg aagattatga cccaggttat ttcaacaatg agagtgacat catcttccaa
     2941 gatcttgaaa aactgaagtc acatccagct tacttggtag tttttctacg ttacatcctc
     3001 tctcaggcag accctggccc cctgcttttt tatttgtgtt cagaagttta tcaacagaca
     3061 aatcccaaag attcccgaag tctggggaaa gacatctgga acattttcct ggagaaaaat
     3121 gcgcctctca gagtgaagat ccctgagatg ttgcaggctg aaattgacct acgcctgcgg
     3181 aacaatgagg accctcgcaa tgtgctctgt gaagctcagg aggcagtcat gctggaaatc
     3241 caggagcaga tcaacgacta cagatccaag cgtactctgg gcctgggcag cctctatggt
     3301 gaaaatgacc tgctaggcct ggatggggac cctcttcgag aacgccaaat ggctgagaag
     3361 cagctggctg cccttggaga tatcttgtcc aaatatgagg aagatcggag tgcccccatg
     3421 gactttgctg ttaatacctt tatgagccac gctgggatcc gtcttcggga gtctcgaggc
     3481 ggatcaggcg gatccatggt cttcacactc gaagatttcg ttggggactg gcgacagaca
     3541 gccggctaca acctggacca agtccttgaa cagggaggtg tgtccagttt gtttcagaat
     3601 ctcggggtgt ccgtaactcc gatccaaagg attgtcctga gcggtgaaaa tgggctgaag
     3661 atcgacatcc atgtcatcat cccgtatgaa ggtctgagcg gcgaccaaat gggccagatc
     3721 gaaaaaattt ttaaggtggt gtaccctgtg gatgatcatc actttaaggt gatcctgcac
     3781 tatggcacac tggtaatcga cggggttacg ccgaacatga tcgactattt cggacggccg
     3841 tatgaaggca tcgccgtgtt cgacggcaaa aagatcactg taacagggac cctgtggaac
     3901 ggcaacaaaa ttatcgacga gcgcctgatc aaccccgacg gctccctgct gttccgagta
     3961 accatcaacg gagtgaccgg ctggcggctg tgcgaacgca ttctggcgta aacgcgtctg
     4021 gaacaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt
     4081 tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc
     4141 ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga
     4201 gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc
     4261 cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct
     4321 ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg
     4381 gctgttgggc actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct ttccatggct
     4441 gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc
     4501 cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg
     4561 tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcctggaat
     4621 taattctgca gtcgagacct agaaaaacat ggagcaatca caagtagcaa tacagcagct
     4681 accaatgctg attgtgcctg gctagaagca caagaggagg aggaggtggg ttttccagtc
     4741 acacctcagg tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt
     4801 ttaaaagaaa agaggggact ggaagggcta attcactccc aacgaagaca agatatcctt
     4861 gatctgtgga tctaccacac acaaggctac ttccctgatt agcagaacta cacaccaggg
     4921 ccaggggtca gatatccact gacctttgga tggtgctaca agctagtacc agttgagcca
     4981 gataaggtag aagaggccaa taaaggagag aacaccagct tgttacaccc tgtgagcctg
     5041 catgggatgg atgacccgga gagagaagtg ttagagtgga ggtttgacag ccgcctagca
     5101 tttcatcacg tggcccgaga gctgcatccg gagtacttca agaactgctg atatcgagct
     5161 tgctacaagg gactttccgc tggggacttt ccagggaggc gtggcctggg cgggactggg
     5221 gagtggcgag ccctcagatc ctgcatataa gcagctgctt tttgcctgta ctgggtctct
     5281 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
     5341 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
     5401 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtagtag
     5461 ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga
     5521 gaggccttga cattgctagc gtttaccgtc gacctctagc tagagcttgg cgtaatcatg
     5581 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
     5641 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
     5701 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
     5761 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
     5821 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     5881 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     5941 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     6001 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     6061 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     6121 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     6181 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     6241 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     6301 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     6361 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     6421 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     6481 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     6541 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     6601 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     6661 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     6721 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
     6781 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
     6841 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
     6901 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
     6961 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
     7021 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
     7081 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
     7141 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
     7201 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
     7261 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
     7321 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
     7381 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
     7441 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
     7501 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
     7561 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
     7621 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
     7681 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcga
     7741 cggatcggga gatcaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
     7801 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
     7861 tcatcaatgt atcttatcat gtctggatca actggataac tcaagctaac caaaatcatc
     7921 ccaaacttcc caccccatac cctattacca ctgccaatta cctgtggttt catttactct
     7981 aaacctgtga ttcctctgaa ttattttcat tttaaagaaa ttgtatttgt taaatatgta
     8041 ctacaaactt agtagttgga agggctaatt cactcccaaa gaagacaaga tatccttgat
     8101 ctgtggatct accacacaca aggctacttc cctgattagc agaactacac accagggcca
     8161 ggggtcagat atccactgac ctttggatgg tgctacaagc tagtaccagt tgagccagat
     8221 aaggtagaag aggccaataa aggagagaac accagcttgt tacaccctgt gagcctgcat
     8281 gggatggatg acccggagag agaagtgtta gagtggaggt ttgacagccg cctagcattt
     8341 catcacgtgg cccgagagct gcatccggag tacttcaaga actgctgata tcgagcttgc
     8401 tacaagggac tttccgctgg ggactttcca gggaggcgtg gcctgggcgg gactggggag
     8461 tggcgagccc tcagatcctg catataagca gctgcttttt gcctgtactg ggtctctctg
     8521 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
     8581 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
     8641 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
     8701 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
     8761 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
     8821 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
     8881 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
     8941 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
     9001 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
     9061 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
     9121 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
     9181 aagaccaccg cacagcaagc ggccggccgc tgatcttcag acctggagga ggagatatga
     9241 gggacaattg gagaagtgaa ttatataaat ataaagtagt aaaaattgaa ccattaggag
     9301 tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca gtgggaatag
     9361 gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca gcgtcaatga
     9421 cgctgacggt acaggccaga caattattgt ctggtatagt gcagcagcag aacaatttgc
     9481 tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc atcaagcagc
     9541 tccaggcaag aatcctggct gtggaaagat acctaaagga tcaacagctc ctggggattt
     9601 ggggttgctc tggaaaactc atttgcacca ctgctgtgcc ttggaatgct agttggagta
     9661 ataaatctct ggaacagatt tggaatcaca cgacctggat ggagtgggac agagaaatta
     9721 acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag caagaaaaga
     9781 atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg tttaacataa
     9841 caaattggct gtggtatata aaattattca taatgatagt aggaggcttg gtaggtttaa
     9901 gaatagtttt tgctgtactt tctatagtga atagagttag gcagggatat tcaccattat
     9961 cgtttcagac ccacctccca accccgaggg gacccgacag gcccgaagga atagaagaag
    10021 aaggtggaga gagagacaga gacagatcca ttcgattagt gaacggatct cgacggtatc
    10081 gcctttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata
    10141 atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt
    10201 cgggtttatt acagggacag cagagatcca gtttatcgat aagcttggga gttccgcgtt
    10261 acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg
    10321 tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg
    10381 gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt
    10441 acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg
    10501 accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg
//
