LOCUS       sequence-370237-       10294 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  GPCR/G protein BRET biosensor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10294)
  AUTHORS   Janicot R, Maziarz M, Park JC, Zhao J, Luebbers A, Green E, 
            Philibert CE, Zhang H, Layne MD, Wu JC, Garcia-Marcos M
  TITLE     Direct interrogation of context-dependent GPCR activity with a 
            universal biosensor platform.
  JOURNAL   Cell. 2024 Feb 16:S0092-8674(24)00065-5. doi: 
            10.1016/j.cell.2024.01.028.
  PUBMED    38412860
REFERENCE   2  (bases 1 to 10294)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2024 
            Feb 16:S0092-8674(24)00065-5. doi: 10.1016/j.cell.2024.01.028."
COMMENT     Sequence Label: pLVX-CMV-Galpha-q ONE-GO
FEATURES             Location/Qualifiers
     source          1..10294
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..204
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     155..175
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     201..225
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             join(727..729,730..732,733..1443)
                     /codon_start=1
                     /product="Venus YFP with monomerizing A206K mutation (Nagai
                     et al., 2002; Kremers et al., 2006)"
                     /label=mVenus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(772..793)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1033..1052)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1380..1401
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    join(2180..2754,2755..2757,2758..2766)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(2347..2364)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     2574..2593
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             2777..2797
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="MGSSKSK"
     CDS             3230..3745
                     /codon_start=1
                     /product="NanoLuc(R) luciferase"
                     /label=Nluc
                     /note="human codon-optimized"
                     /translation="MVFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQR
                     IVLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGV
                     TPNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGW
                     RLCERILA"
     misc_feature    3759..4347
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3812..3832)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4230..4241)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4554..5187
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(5315..5331)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5315..5331)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5328..5350)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5339..5355
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5363..5369,5370..5387,5388..5393))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5408..5429
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5546..5563)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5717..6305)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5797..5816)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6476..7267,7268..7336))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7099..7118
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7337..7441)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    7489..7623
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7526..7545)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7580..7599
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             7791..8424
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    8471..8596
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9093..9326
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9511..9555
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9818..9933
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     enhancer        9991..10294
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
       61 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
      121 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
      181 tgggaggtct atataagcag agctcgttta gtgaaccgtc agatcgcctg gagacgccat
      241 ccacgctgtt ttgacctcca tagaagacac cgactctact agaggatcta tttccggtga
      301 attcctcgag actagttcta gagcggccgc ggatccatga ctctggagtc catcatggcg
      361 tgctgcctga gcgaggaggc caaggaagcc cggaggatca acgacgagat cgagcggcag
      421 ctgcgcaggg acaagcgcga cgcccgccgg gaactcaagc tgctgctgct ggggacaggg
      481 gagagtggca agagcacctt catcaagcag atgaggatca tccacgggtc gggctactct
      541 gacgaagaca agcgcggctt caccaagctg gtgtatcaga acatcttcac ggccatgcag
      601 gccatgatca gagcgatgga cacactcaag atcccataca agtatgaaca caataaggct
      661 catgcacaat tggttcgaga ggttgatgtg gagaaggtgt ctgctttttc aggaggtggc
      721 ggatccatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag
      781 ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc
      841 acctacggca agctgaccct gaagctgatc tgcaccaccg gcaagctgcc cgtgccctgg
      901 cccaccctcg tgaccaccct gggctacggc ctgcagtgct tcgcccgcta ccccgaccac
      961 atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc
     1021 atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac
     1081 accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg
     1141 gggcacaagc tggagtacaa ctacaacagc cacaacgtct atatcaccgc cgacaagcag
     1201 aagaacggca tcaaggccaa cttcaagatc cgccacaaca tcgaggacgg cggcgtgcag
     1261 ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac
     1321 aaccactacc tgagctacca gtccaaactg agcaaagacc ccaacgagaa gcgcgatcac
     1381 atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac
     1441 aagtctggcg gtggaggatc cgagaatcca tatgtagatg caataaagag cttgtggaat
     1501 gatcctggaa tccaggagtg ctacgacaga cgacgggaat atcagttatc tgactctacc
     1561 aaatactatc tgaatgactt ggaccgtgta gccgaccctg agtacatgcc gacagagcaa
     1621 gacgtgctta gagttcgagt ccccactaca gggatcatcg aatacccctt tgacttacaa
     1681 agtgtcattt tcagaatggt cgatgtaggg ggccaaaggt cagagagaag aaaatggata
     1741 cactgctttg aaaatgtcac ctccatcatg tttctagtag cgcttagcga atatgatcaa
     1801 gttcttgtgg agtcagacaa tgagaaccgc atggaggaga gcaaagcact ctttagaaca
     1861 attatcacct acccctggtt ccagaactcc tctgtgattc tgttcttaaa caagaaagat
     1921 cttctagagg agaaaatcat gtattcccac ctagtcgact acttcccaga atatgatgga
     1981 ccccagagag atgcccaggc agctcgagaa ttcatcctga aaatgttcgt ggacctgaac
     2041 cccgacagtg acaaaatcat ctactcccac ttcacgtgcg ccacagatac cgagaacatc
     2101 cgcttcgtct ttgcagccgt caaggacacc atcctgcagc tgaacctgaa ggagtacaat
     2161 ctggtctaag cggccgccgc ccctctccct cccccccccc taacgttact ggccgaagcc
     2221 gcttggaata aggccggtgt gcgtttgtct atatgttatt ttccaccata ttgccgtctt
     2281 ttggcaatgt gagggcccgg aaacctggcc ctgtcttctt gacgagcatt cctaggggtc
     2341 tttcccctct cgccaaagga atgcaaggtc tgttgaatgt cgtgaaggaa gcagttcctc
     2401 tggaagcttc ttgaagacaa acaacgtctg tagcgaccct ttgcaggcag cggaaccccc
     2461 cacctggcga caggtgcctc tgcggccaaa agccacgtgt ataagataca cctgcaaagg
     2521 cggcacaacc ccagtgccac gttgtgagtt ggatagttgt ggaaagagtc aaatggctct
     2581 cctcaagcgt attcaacaag gggctgaagg atgcccagaa ggtaccccat tgtatgggat
     2641 ctgatctggg gcctcggtgc acatgcttta catgtgttta gtcgaggtta aaaaaacgtc
     2701 taggcccccc gaaccacggg gacgtggttt tcctttgaaa aacacgatga taagcttgcc
     2761 acaacccgta ccaaagatgg ggagtagcaa gagcaagacg tcgaattccg gaatcaagct
     2821 taagtacctg gaggaccggg gcgaggtgac ttttgagaag atcttctccc agaagctggg
     2881 gtacctgctt ttccgagact tctgcctgaa gcacctggag gaggccaagc ccttggtaga
     2941 gttctacgag gagatcaaga aatacgagaa gctggagaca gaggaggagc gcctggtctg
     3001 cagccgagag atcttcgaca cgtacatcat gaaggagctg ctggcctgct cacatccttt
     3061 ctcgaagagc gccattgagc acgtccaggg ccatctggtg aagaagcagg tgcctccgga
     3121 tctcttccag ccatatattg aagaaatttg ccagaacctc cgaggagacg tgttccagaa
     3181 attcatcgag agcgataaat tcacacggtt ttgccagtgg aagggatcca tggtcttcac
     3241 actcgaagat ttcgttgggg actggcgaca gacagccggc tacaacctgg accaagtcct
     3301 tgaacaggga ggtgtgtcca gtttgtttca gaatctcggg gtgtccgtaa ctccgatcca
     3361 aaggattgtc ctgagcggtg aaaatgggct gaagatcgac atccatgtca tcatcccgta
     3421 tgaaggtctg agcggcgacc aaatgggcca gatcgaaaaa atttttaagg tggtgtaccc
     3481 tgtggatgat catcacttta aggtgatcct gcactatggc acactggtaa tcgacggggt
     3541 tacgccgaac atgatcgact atttcggacg gccgtatgaa ggcatcgccg tgttcgacgg
     3601 caaaaagatc actgtaacag ggaccctgtg gaacggcaac aaaattatcg acgagcgcct
     3661 gatcaacccc gacggctccc tgctgttccg agtaaccatc aacggagtga ccggctggcg
     3721 gctgtgcgaa cgcattctgg cgtaaacgcg tctggaacaa tcaacctctg gattacaaaa
     3781 tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg
     3841 ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct
     3901 tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg
     3961 gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct
     4021 gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg
     4081 ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg
     4141 tgttgtcggg gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc acctggattc
     4201 tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc
     4261 gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc
     4321 ggatctccct ttgggccgcc tccccgcctg gaattaattc tgcagtcgag acctagaaaa
     4381 acatggagca atcacaagta gcaatacagc agctaccaat gctgattgtg cctggctaga
     4441 agcacaagag gaggaggagg tgggttttcc agtcacacct caggtacctt taagaccaat
     4501 gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaagaggg gactggaagg
     4561 gctaattcac tcccaacgaa gacaagatat ccttgatctg tggatctacc acacacaagg
     4621 ctacttccct gattagcaga actacacacc agggccaggg gtcagatatc cactgacctt
     4681 tggatggtgc tacaagctag taccagttga gccagataag gtagaagagg ccaataaagg
     4741 agagaacacc agcttgttac accctgtgag cctgcatggg atggatgacc cggagagaga
     4801 agtgttagag tggaggtttg acagccgcct agcatttcat cacgtggccc gagagctgca
     4861 tccggagtac ttcaagaact gctgatatcg agcttgctac aagggacttt ccgctgggga
     4921 ctttccaggg aggcgtggcc tgggcgggac tggggagtgg cgagccctca gatcctgcat
     4981 ataagcagct gctttttgcc tgtactgggt ctctctggtt agaccagatc tgagcctggg
     5041 agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc
     5101 ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct
     5161 tttagtcagt gtggaaaatc tctagcagta gtagttcatg tcatcttatt attcagtatt
     5221 tataacttgc aaagaaatga atatcagaga gtgagaggcc ttgacattgc tagcgtttac
     5281 cgtcgacctc tagctagagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt
     5341 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg
     5401 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
     5461 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
     5521 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     5581 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     5641 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     5701 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     5761 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     5821 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     5881 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     5941 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     6001 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     6061 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     6121 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
     6181 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     6241 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     6301 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     6361 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
     6421 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
     6481 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     6541 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     6601 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
     6661 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     6721 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     6781 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     6841 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     6901 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     6961 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     7021 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     7081 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
     7141 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
     7201 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
     7261 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
     7321 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
     7381 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
     7441 gcacatttcc ccgaaaagtg ccacctgacg tcgacggatc gggagatcaa cttgtttatt
     7501 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
     7561 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg
     7621 atcaactgga taactcaagc taaccaaaat catcccaaac ttcccacccc ataccctatt
     7681 accactgcca attacctgtg gtttcattta ctctaaacct gtgattcctc tgaattattt
     7741 tcattttaaa gaaattgtat ttgttaaata tgtactacaa acttagtagt tggaagggct
     7801 aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca cacaaggcta
     7861 cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac tgacctttgg
     7921 atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca ataaaggaga
     7981 gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg agagagaagt
     8041 gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag agctgcatcc
     8101 ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg ctggggactt
     8161 tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat cctgcatata
     8221 agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga gcctgggagc
     8281 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
     8341 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
     8401 agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag cgaaagggaa
     8461 accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga
     8521 ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga aggagagaga
     8581 tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg
     8641 gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg caagcaggga
     8701 gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct gtagacaaat
     8761 actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat cattatataa
     8821 tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca ccaaggaagc
     8881 tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc aagcggccgg
     8941 ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag tgaattatat
     9001 aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc aaagagaaga
     9061 gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg gttcttggga
     9121 gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc cagacaatta
     9181 ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc gcaacagcat
     9241 ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct ggctgtggaa
     9301 agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa actcatttgc
     9361 accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca gatttggaat
     9421 cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt aatacactcc
     9481 ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt ggaattagat
     9541 aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta tataaaatta
     9601 ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt actttctata
     9661 gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct cccaaccccg
     9721 aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga cagagacaga
     9781 tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag gggggattgg
     9841 ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac aaactaaaga
     9901 attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg acagcagaga
     9961 tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa atggcccgcc
    10021 tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt
    10081 aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca
    10141 cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg
    10201 taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca
    10261 gtacatctac gtattagtca tcgctattac catg
//
