LOCUS       sequence-37024-e         918 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 918)
  AUTHORS   Senkel S, Waldner C, Ryffel GU, Thomas H
  TITLE     Improved conditional expression systems resulting in physiological 
            level of HNF4alpha expression confirm HNF4alpha induced apoptosis in
            the pancreatic beta-cell line INS-1.
  JOURNAL   BMC Res Notes. 2009 Oct 17;2:210. doi: 10.1186/1756-0500-2-210.
  PUBMED    19835622
REFERENCE   2  (bases 1 to 918)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Res 
            Notes."; date: "2009-10-17"; volume: "2:210. doi"; pages: " 
            10.1186/1756-0500-2-210"
COMMENT     Sequence Label: CMV-68_HNF4A2
FEATURES             Location/Qualifiers
     source          1..918
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    20..38
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    41..59
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     69..93
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             259..288
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             298..327
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             337..366
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             376..405
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             415..444
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             478..507
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 cgggggnttt agcgagctct ccctatcagt gatagagatc tccctatcag tgatagagat
       61 cgtcgacgag ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt
      121 gacctccata gaagacaccg ggaccgatcc agcctccgga ctctagcgtt taaacttaag
      181 cttggtaccg agctcggatc cactagtcca gtgtggtgga attctgcaga tatccagcac
      241 agtggcggcc gcgctatgga gcaaaagctc atttctgaag aggacttgaa tgaaatggag
      301 caaaagctca tttctgaaga ggacttgaat gaaatggagc aaaagctcat ttctgaagag
      361 gacttgaatg aaatggagca aaagctcatt tctgaagagg acttgaatga aatggagcaa
      421 aagctcattt ctgaagagga cttgaatgaa atggagagct tgggcgacct caccatggag
      481 caaaagctca tttctgaaga ggacttgaat tcaaggcctg gtaccatgcg actctccaaa
      541 accctcgtcg acatggacat ggccgactac agtgctgcac tggacccagc ctacaccacc
      601 ctggaatttg agaatgtgca ggtgttgacg atgggcaatg acacgtcccc atcagaaggc
      661 accaacctca acgcgcccaa cagcctgggt gtcagcgccc tgtgtgccat ctgcggggac
      721 cgggccacgg gcaaacacta cggtgcctcg agctgtgacg gctgcaaggg cttcttccgg
      781 aggagcgtgc ggaagaacca catgtactcc tgcagattta gccggcagtg cgtggtggac
      841 aaagacaaga ggaaccagtg ccgctactgc aggctcaaga aatgcttccg ggctggcatg
      901 aagaaggaag ccgtccaa
//
