LOCUS       sequence-37026-e         929 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 929)
  AUTHORS   Senkel S, Waldner C, Ryffel GU, Thomas H
  TITLE     Improved conditional expression systems resulting in physiological 
            level of HNF4alpha expression confirm HNF4alpha induced apoptosis in
            the pancreatic beta-cell line INS-1.
  JOURNAL   BMC Res Notes. 2009 Oct 17;2:210. doi: 10.1186/1756-0500-2-210.
  PUBMED    19835622
REFERENCE   2  (bases 1 to 929)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Res 
            Notes."; date: "2009-10-17"; volume: "2:210. doi"; pages: " 
            10.1186/1756-0500-2-210"
COMMENT     Sequence Label: CMV-138_HNF4A8
FEATURES             Location/Qualifiers
     source          1..929
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    19..37
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    40..58
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     68..92
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             258..287
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             297..326
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             336..365
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             375..404
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             414..443
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             477..506
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 cgngggcttt agcgactctc cctatcagtg atagagatct ccctatcagt gatagagatc
       61 gtcgacgagc tcgtttagtg aaccgtcaga tcgcctggag acgccatcca cgctgttttg
      121 acctccatag aagacaccgg gaccgatcca gcctccggac tctagcgttt aaacttaagc
      181 ttggtaccga gctcggatcc actagtccag tgtggtggaa ttctgcagat atccagcaca
      241 gtggcggccg cgctatggag caaaagctca tttctgaaga ggacttgaat gaaatggagc
      301 aaaagctcat ttctgaagag gacttgaatg aaatggagca aaagctcatt tctgaagagg
      361 acttgaatga aatggagcaa aagctcattt ctgaagagga cttgaatgaa atggagcaaa
      421 agctcatttc tgaagaggac ttgaatgaaa tggagagctt gggcgacctc accatggagc
      481 aaaagctcat ttctgaagag gacttgaatt caaggcctgg taccatggtc agcgtgaacg
      541 cgcccctcgg ggctccagtg gagagttctt acgacacgtc cccatcagaa ggcaccaacc
      601 tcaacgcgcc caacagcctg ggtgtcagcg ccctgtgtgc catctgcggg gaccgggcca
      661 cgggcaaaca ctacggtgcc tcgagctgtg acggctgcaa gggcttcttc cggaggagcg
      721 tgcggaagaa ccacatgtac tcctgcagat ttagccggca gtgcgtggtg gacaaagaca
      781 agaggaacca gtgccgctac tgcaggctca agaaatgctt ccgggctggc atgaagaagg
      841 aagccgtcca gaatgagcgg gaccggatca gcactcgaag gtcaagctat gaggacagca
      901 gcctgccctc catcaatgcg ctcctgcag
//
