LOCUS       sequence-370299-       13794 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes a promoter-less Bacteroides-optimized lux cassette.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13794)
  AUTHORS   Modesto JL, Pearce VH, Townsend GE 2nd
  TITLE     Harnessing gut microbes for glycan detection and quantification.
  JOURNAL   Nat Commun. 2023 Jan 17;14(1):275. doi: 10.1038/s41467-022-35626-2.
  PUBMED    36650134
REFERENCE   2  (bases 1 to 13794)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-01-17"; volume: "14(1):275. doi"; pages: " 
            10.1038/s41467-022-35626-2"
COMMENT     Sequence Label: pBolux
FEATURES             Location/Qualifiers
     source          1..13794
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     502..520
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(558..580)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     680..699
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3601..3623
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3615..3632
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3616..3632
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             3715..5157
                     /codon_start=1
                     /gene="luxC"
                     /product="LuxC fatty acid reductase"
                     /label=LuxC
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MTKKISFIINGQVEIFPEGDDLVQSINFGDNSVYLPILNDSHVKN
                     IIDCNGNNELRLHNIVNFLYTVGQRWKNEEYSRRRTYIRDLKKYMGYSEEMAKLEANWI
                     SMILCSKGGLYDVVENELGSRHIMDEWLPQDESYVRAFPKGKSVHLLAGNVPLSGIMSI
                     LRAILTKNQCIIKTSSTDPFTANALALSFIDVDPNHPITRSLSVIYWPHQGDTSLAKEI
                     MQHADVIVAWGGPDAINWAVEHAPSYADVIKFGSKKSLCIIDNPVDLTSAATGAAHDVC
                     FYDQRACFSAQNIYYMGNHYEEFKLALIEKLNLYAHILPNAKKDFDEKAAYSLVQKESL
                     FAGLKVEVDIHQRWMIIESNAGVEFNQPLGRCVYLHHVDNIEQILPYVQKNKTQTISIF
                     PWESSFKYRDALALKGAERIVEAGMNNIFRVGGSHDGMRPLQRLVTYISHERPSNYTAK
                     DVAVEIEQTRFLEEDKFLVFVP"
     CDS             7346..8425
                     /codon_start=1
                     /gene="luxA"
                     /product="LuxA luciferase subunit"
                     /label=LuxA
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MKFGNFLLTYQPPQFSQTEVMKRLVKLGRISEECGFDTVWLLEHH
                     FTEFGLLGNPYVAAAYLLGATKKLNVGTAAIVLPTAHPVRQLEDVNLLDQMSKGRFRFG
                     ICRGLYNKDFRVFGTDMNNSRALAECWYGLIKNGMTEGYMEADNEHIKFHKVKVNPAAY
                     SRGGAPVYVVAESASTTEWAAQFGLPMILSWIINTNEKKAQLELYNEVAQEYGHDIHNI
                     DHCLSYITSVDHDSIKAKEICRKFLGHWYDSYVNATTIFDDSDQTRGYDFNKGQWRDFV
                     LKGHKDTNRRIDYSYEINPVGTPQECIDIIQKDIDATGISNICCGFEANGTVDEIIASM
                     KLFQSDVMPFLKEKQRSLL"
     primer_bind     complement(9459..9475)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9459..9475)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9472..9494)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9483..9499
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9507..9513,9514..9531,9532..9537))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9552..9573
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9690..9707)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9861..10449)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9941..9960)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10620..11411,11412..11480))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11243..11262
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11481..11585)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 actgccgcat aacgattaca aaattacaca aaaaaatata tctaacaaaa gtaggaggat
       61 tttttaactt tttaccatag acattttatt agggaaacgt aggttcaact ggtaagtaca
      121 aataagtaga aatgtttgaa caacactgtt ttaggaagtt gaaaaatcaa gtttctctct
      181 cggtatagcg tttattttgg ccaatatctt ctttagtttg gcatttgagt atttcccatt
      241 cgtgttctat ttagctcctt attatgagta tgtagcaagt tgcttatcaa attcagcctc
      301 tttgagggtc aaatatggtt ttgcaaatgg tgactgacaa gacataaaca aggtcagcag
      361 gattttttac ataaatggac atgaatgtat ataacggggt ttgagggcca atggaacgaa
      421 aacgtacgtt aaggagataa ttcgttgttt atatttaaat ttagctaaga aaccattatt
      481 atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtct cgcgcgtttc
      541 ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg
      601 taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt
      661 cggggctggc ttaactatgc ggcatcagag cagattgtac tgagagtgca ccattccccg
      721 accgatgatt tttaagtgac tgattttgtg ctgttttggg ggtatattaa gaataaaaga
      781 aatagaataa gttaagtact tgatacacaa tatagggcat tttccatatt ggaaattctc
      841 attttccaat ctagaaaata ccgatttcct aatatgatac taaataggaa aataatattt
      901 cccttaatat tgtttttatg gaaaataata gtttactttg tggagaataa tatttcccaa
      961 aaacatatca aaatggaaaa taaaaaagca gttaagttaa ccgattttca aaagaacgaa
     1021 gaaaatcctt ttatgaaaca agctatagaa ggtattgaaa atcatgttgt taaaaagtat
     1081 aagagtaata gtggtggcga taagagagct gtagtagctt tagccgacac tgaaactgga
     1141 gaagtgttta agacttcgtt tatccgtcaa atagaagtag atgaagaaca attcactaaa
     1201 ttgtatcttt ctaactttgc tgcattcttt gacctatcac aagcagctat tcgggttttt
     1261 ggttacttta tgacctgcat gaaacccaaa aatgatttaa tcattttcaa tagaaaaaaa
     1321 tgcctagaat ataccaaata caaaacagac aaagccgttt ataaaggact tgcagaactt
     1381 gtaaaagctg aaatcatagc ccgaggacca gccgataatc tttggtttat taatcctctg
     1441 atagtattca atggtgaccg agtgacattt gctaaaacat acgttcggaa aaagacttta
     1501 gctgcccaaa agaaagaaga agcagagaaa cgacaattat cacttggctt tgatgaacag
     1561 taacactcca ttgagtgaag ctgccgtttg gtcgctcccc ctttgggggc ggggggggat
     1621 agataaagtt cctctatgta aagttataat gggggatgaa aggcaaggtt cgctaacctt
     1681 accccgattg tttatgcttc ccagcattca atctatcccc gtttatgggt ttctctttca
     1741 ccctacaaag ataacctcat gggggaaaaa tgtccaagaa tatggggtaa aaactatcaa
     1801 gtcggtagaa aataagtatc tttgaagtac atttttagta gaggtactgg tgtgcctagg
     1861 cgaagcagat aggcaaaaat aaagccgctg gagatgtcgg atgtccgtca attaactcca
     1921 gcggcttgta ttttggtaac tctcccgtat cggtcagcta ccgtttggtc agcaacaaag
     1981 gtagtacttt attatcgcaa atccaaatgt ttttcccttt atttgtgcat ttacaaaaca
     2041 ttcactaact tcgcacctat gcagtatggt gatagcacac tagcacccta aagagtgcgt
     2101 gtgtaagggg acaagccccc ttaaccccct gtcagccggc tgtcaccggc tgttatcgat
     2161 tgtcacaaaa atgttaactt atgggagcaa caagtattca tgtacaagca gtgaagccgg
     2221 ggagcgagat tcacaacttt agggaaaaag agttggacta tgttcgtccc gaacttagtc
     2281 atttgaatga aagctgggtt ggagatagca tttcccatcg gctggagagt gcaaaacaaa
     2341 gatatctcga tacggttggg cagaagatgc aggctaaagc cgcacccata cgagagggag
     2401 taatagtaat caaacaagaa accactatgc aggaactcca gcagtttgcc acggtctgca
     2461 aagaacgttt cggtatcgaa gcgtttcaaa tccatataca caaggacgaa ggatacatga
     2521 acgcaaagca gtggacacct aaccttcatg cccatgtagt tttcgattgg acgcagccga
     2581 atgggaaaag tgtgcgttta tcgcgtgatg acatggcaga actccaaacc atagcatctg
     2641 aaacactggg catggaacgt ggcgtttctt ctgaccgcaa acacttatcg gctatgcagt
     2701 acaagaccga atgtgcgaaa gaacagcttc aggaactatc caacgatata tccagtgcat
     2761 tagacaaata caaggacgca caaaaccagc ttcttcagct tcagaaggaa ctacgttcca
     2821 ttgaaacaaa gaagaacgtc caaaaactca tttctaaggc ttcagagaag ttttacggct
     2881 tgattggaaa aacagtcaac gatagggaaa aggatgcctt aaaagccaaa gtaaagactt
     2941 tagaggggga aaatgaacaa ctatccgata gactgggaaa ggctatactt gaaaaagagc
     3001 gaaacagcac aaaggcattc aaagccgaga atgacaagga gtattataga caacaaatgg
     3061 acaacgcaag gacaaccagt aacctattaa ggacggagaa ccaaaaacta aaagccgaaa
     3121 ccaaggagtt gaaaaaagaa cttggtaaga tgaaagactt gttcaattcc gaacaactgg
     3181 aggctttaag gcatcatttc ccaaacatat caaaagctat ggaagagggg aaagacctat
     3241 tcaagcaaat caccagaagc agaggtttcg gcatgggtat gtaacccccc cccgccccca
     3301 aagggggagc gaccaaacgg cagcctctct caatggagtg ttacaccgtt cggattcaga
     3361 aaacattttg cagggttcgg aaaacggttg tgtgcttatt ctaaaaaaag tttatggtgt
     3421 gtatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcgccatt
     3481 cgccattcag gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac
     3541 gccagctggc gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt
     3601 cccagtcacg acgttgtaaa acgacggcca gtgaattcga gctcggtacc cggggatcca
     3661 ctagtcactc ccgcatttta aaataaaata aattatttat ttaattaaac gaatatgact
     3721 aaaaaaattt cattcattat taacggccag gttgaaatct ttcccgaagg tgatgattta
     3781 gtgcaatcca ttaattttgg tgataatagt gtttacctgc caatattgaa tgactctcat
     3841 gtaaaaaaca ttattgattg taatggaaat aacgaattac ggttgcataa cattgtcaat
     3901 tttctctata cggtagggca aagatggaaa aatgaagaat actcaagacg caggacatac
     3961 attcgtgact taaaaaaata tatgggatat tcagaagaaa tggctaagct agaggccaat
     4021 tggatatcta tgattttatg ttctaaaggc ggcctttatg atgttgtaga aaatgaactt
     4081 ggttctcgcc atatcatgga tgaatggcta cctcaggatg aaagttatgt tcgggctttt
     4141 ccgaaaggta aatctgtaca tctgttggca ggtaatgttc cattatctgg gatcatgtct
     4201 atattacgcg caattttaac taagaatcag tgtattataa aaacatcgtc aaccgatcct
     4261 tttaccgcta atgcattagc gttaagtttt attgatgtag accctaatca tccgataacg
     4321 cgctctttat ctgttatata ttggccccac caaggtgata catcactcgc aaaagaaatt
     4381 atgcaacatg cggatgttat tgtcgcttgg ggagggccag atgcgattaa ttgggcggta
     4441 gagcatgcgc catcttatgc tgatgtgatt aaatttggtt ctaaaaagag tctttgcatt
     4501 atcgataatc ctgttgattt gacgtccgca gcgacaggtg cggctcatga tgtttgtttt
     4561 tacgatcagc gagcttgttt ttctgcccaa aacatatatt acatgggaaa tcattatgag
     4621 gaatttaagt tagcgttgat agaaaaactt aatctatatg cgcatatatt accgaatgcc
     4681 aaaaaagatt ttgatgaaaa ggcggcctat tctttagttc aaaaagaaag cttgtttgct
     4741 ggattaaaag tagaggtgga tattcatcaa cgttggatga ttattgagtc aaatgcaggt
     4801 gtggaattta atcaaccact tggcagatgt gtgtaccttc atcacgtcga taatattgag
     4861 caaatattgc cttatgttca aaaaaataag acgcaaacca tatctatttt tccttgggag
     4921 tcatcattta aatatcgaga tgcgttagca ttaaaaggtg cggaaaggat tgtagaagca
     4981 ggaatgaata acatatttcg agttggtgga tctcatgacg gaatgagacc gttgcaacga
     5041 ttagtgacat atatttctca tgaaaggcca tctaactata cggctaagga tgttgcggtt
     5101 gaaatagaac agactcgatt cctggaagaa gataagttcc ttgtatttgt cccgtaatcc
     5161 gcatggcaaa ttgtaaattg taaatcgtaa aatagtaata tattaatgga aaataaatcc
     5221 aaatataaaa ccatcgacca cgttatttgt gttgaaggaa ataaaaaaat tcatgtttgg
     5281 gaaacgctgc cagaagaaaa cagcccaaag agaaagaatg ccattattat tgcgtctggt
     5341 tttgcccgca ggatggatca ttttgctggt ctggcggaat atttatcgcg gaatggattt
     5401 catgtgatcc gctatgattc gcttcaccac gttggattga gttcagggac aattgatgaa
     5461 tttacaatgt ctataggaaa gcagagcttg ttagcagtgg ttgattggtt aactacacga
     5521 aaaataaata acttcggtat gttggcttca agcttatctg cgcggatagc ttatgcaagc
     5581 ctatctgaaa tcaatgcttc gtttttaatc accgcagtcg gtgttgttaa cttaagatat
     5641 tctcttgaaa gagctttagg gtttgattat ctcagtctac ccattaatga attgccgaat
     5701 aatctagatt ttgaaggcca taaattgggt gctgaagtct ttgcgagaga ttgtcttgat
     5761 tttggttggg aagatttagc ttctacaatt aataacatga tgtatcttga tataccgttt
     5821 attgctttta ctgcaaataa cgataattgg gtcaagcaag atgaagttat cacattgtta
     5881 tcaaatattc gtagtaatcg atgcaagata tattctttgt taggaagttc gcatgacttg
     5941 agtgaaaatt tagtggtcct gcgcaatttt tatcaatcgg ttacgaaagc cgctatcgcg
     6001 atggataatg atcatctgga tattgatgtt gatattactg aaccgtcatt tgaacattta
     6061 actattgcga cagtcaatga acgccgaatg agaattgaga ttgaaaatca agcgatttcg
     6121 ctgtcttaat aaggaggagg ataaagaata tgacttcata tgttgataaa caagaaatta
     6181 cagcaagctc agaaattgat gatttgattt tttcgagcga tccattagtg tggtcttacg
     6241 acgagcagga aaaaatcaga aagaaacttg tgcttgatgc atttcgtaat cattataaac
     6301 attgtcgaga atatcgtcac tactgtcagg cacacaaagt agatgacaat attacggaaa
     6361 ttgatgacat acctgtattc ccaacatcgg tttttaagtt tactcgctta ttaacttctc
     6421 aggaaaacga gattgaaagt tggtttacca gtagcggcac gaatggttta aaaagtcagg
     6481 tggcgcgtga cagattaagt attgagagac tcttaggctc tgtgagttat ggcatgaaat
     6541 atgttggtag ttggtttgat catcaaatag aattagtcaa tttgggacca gatagattta
     6601 atgctcataa tatttggttt aaatatgtta tgagtttggt ggaattgtta tatcctacga
     6661 catttaccgt aacagaagaa cgaatagatt ttgttaaaac attgaatagt cttgaacgaa
     6721 taaaaaatca agggaaagat ctttgtctta ttggttcgcc atactttatt tatttactct
     6781 gccattatat gaaagataaa aaaatctcat tttctggaga taaaagcctt tatatcataa
     6841 ccggaggcgg ctggaaaagt tacgaaaaag aatctctgaa acgtgatgat ttcaatcatc
     6901 ttttatttga tactttcaat ctcagtgata ttagtcagat ccgagatata tttaatcaag
     6961 ttgaactcaa cacttgtttc tttgaggatg aaatgcagcg taaacatgtt ccgccgtggg
     7021 tatatgcgcg agcgcttgat cctgaaacgt tgaaacctgt acctgatgga acgccggggt
     7081 tgatgagtta tatggatgcg tcagcaacca gttatccagc atttattgtt accgatgatg
     7141 tcgggataat tagcagagaa tatggtaagt atcccggcgt gctcgttgaa attttacgtc
     7201 gcgtcaatac gaggacgcag aaagggtgtg ctttaagctt aaaccaagca tttaatagtt
     7261 gatataaatg aagggtgaag aaggatgaat gaaggtaaca ctcataaact cgaaattctt
     7321 cattcttaat ttttaattaa aatatatgaa atttggaaac tttttgctta cataccaacc
     7381 tccccaattt tctcaaacag aggtaatgaa acgtttggtt aaattaggtc gcatctctga
     7441 ggagtgtggt tttgataccg tatggttact ggagcatcat ttcacggagt ttggtttgct
     7501 tggtaaccct tatgtcgctg ctgcatattt acttggcgcg actaaaaaat tgaatgtagg
     7561 aactgccgct attgttcttc ccacagccca tccagtacgc caacttgaag atgtgaattt
     7621 attggatcaa atgtcaaaag gacgatttcg gtttggtatt tgccgagggc tttacaacaa
     7681 ggactttcgc gtattcggca cagatatgaa taacagtcgc gccttagcgg aatgctggta
     7741 cgggctgata aagaatggca tgacagaggg atatatggaa gctgataatg aacatatcaa
     7801 gttccataag gtaaaagtaa accccgcggc gtatagcaga ggtggcgcac cggtttatgt
     7861 ggtggctgaa tcagcttcga cgactgagtg ggctgctcaa tttggcctac cgatgatatt
     7921 aagttggatt ataaatacta acgaaaagaa agcacaactt gagctttata atgaagtggc
     7981 tcaagaatat gggcacgata ttcataatat cgaccattgc ttatcatata taacatctgt
     8041 agatcatgac tcaattaaag cgaaagagat ttgccggaaa tttctggggc attggtatga
     8101 ttcttatgtg aatgctacga ctatttttga tgattcagac caaacaagag gttatgattt
     8161 caataaaggg cagtggcgtg actttgtatt aaaaggacat aaagatacta atcgccgtat
     8221 tgattacagt tacgaaatca atcccgtggg aacgccgcag gaatgtattg acataattca
     8281 aaaagacatt gatgctacag gaatatcaaa tatttgttgt ggatttgaag ctaatggaac
     8341 agtagacgaa attattgctt ccatgaagct cttccagtct gatgtcatgc catttcttaa
     8401 agaaaaacaa cgttcgctat tataaagaaa ggaataagac aatggatatg aaatttggat
     8461 tgttcttcct taacttcatc aattcaacaa ctgttcaaga acaaagtata gttcgcatgc
     8521 aggaaataac ggagtatgtt gataagttga attttgaaca gattttagtg tatgaaaatc
     8581 atttttcaga taatggtgtt gtcggcgctc ctctgactgt ttctggtttt ctgctcggtt
     8641 taacagagaa aattaaaatt ggttcattaa atcacatcat tacaactcat catcctgtcc
     8701 gcatagcgga ggaagcttgc ttattggatc agttaagtga agggagattt attttagggt
     8761 ttagtgattg cgaaaaaaaa gatgaaatgc atttttttaa tcgcccggtt gaatatcaac
     8821 agcaactatt tgaagagtgt tatgaaatca ttaacgatgc tttaacaaca ggctattgta
     8881 atccagataa cgatttttat agcttcccta aaatatctgt aaatccccat gcttatacgc
     8941 caggcggacc tcggaaatat gtaacagcaa ccagtcatca tattgttgag tgggcggcca
     9001 aaaaaggtat tcctctcatc tttaagtggg atgattctaa tgatgttaga tatgaatatg
     9061 ctgaaagata taaagccgtt gcggataaat atgacgttga cctatcagag atagaccatc
     9121 agttaatgat attagttaac tataacgaag atagtaataa agctaaacaa gagacgcgtg
     9181 catttattag tgattatgtt cttgaaatgc accctaatga aaatttcgaa aataaacttg
     9241 aagaaataat tgcagaaaac gctgtcggaa attatacgga gtgtataact gcggctaagt
     9301 tggcaattga aaagtgtggt gcgaaaagtg tattgctgtc ctttgaacca atgaatgatt
     9361 tgatgagcca aaaaaatgta atcaatattg ttgatgataa tattaaaaag taccacatgt
     9421 aagtcgacct gcaggcatgc aagcttggcg taatcatggt catagctgtt tcctgtgtga
     9481 aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc
     9541 tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc
     9601 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc
     9661 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
     9721 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
     9781 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     9841 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     9901 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     9961 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
    10021 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
    10081 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
    10141 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
    10201 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
    10261 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc
    10321 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
    10381 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
    10441 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
    10501 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
    10561 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
    10621 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
    10681 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
    10741 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
    10801 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
    10861 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
    10921 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
    10981 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
    11041 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
    11101 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
    11161 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
    11221 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
    11281 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
    11341 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
    11401 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
    11461 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
    11521 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
    11581 ccgcgcacat ttccccgaaa agtgccacct gcgtctattt ttttattgcc aagttctaat
    11641 gcttctatct cctgccattc atagaggcta ttatatgcca tacatccgat aagaatggag
    11701 ataagtatat atcccaactg tattgcctta tagaaatttc ctgaccgctc cattattttg
    11761 atgacattga tttttggaac atgaataaaa gtttatcttt ttcgttcatg cggatattat
    11821 cagaataacc gccttttgtt atttgatacc cgcatggctt aaccataaaa atgcctaagc
    11881 ccttagtgta tgaacttact tctgatgcat agtctttact tgtatttaat ggaactttcc
    11941 ctttaatatg acaccactca ttattgcaac tgatgtcttc aatctcagac atcatttttt
    12001 gcaaatctgt aatagctttg gaacttgccg cttggggtat ctgcaactca aaatagagca
    12061 tcggttcgag aatgtccaca cctgactgtt gcaaggccag cctgaagaca taaggggtca
    12121 gctgtctgaa atcagcaggt gtacttaccg ggctataata ctcggcttga gtaaaagtta
    12181 ctttcagatc agtcacttcc catccatgta acccggattg gcaagacata cgaatccctt
    12241 caaaaacggc attttgaaaa gaatggttca gataaccata ggagatgtca ctttcgattt
    12301 gcaaccctgt ccctaacggt aagggttcaa gagtcagccc tattgtggcc caataagggt
    12361 tgggcggcac ttcgatctga ataatcttat tgaccttttt tacaggtcgt tctttgtata
    12421 tagtcttgat ctcatcaaaa tggaccttta cggaaaatcg ttcttccagc aatgtctgta
    12481 tgatttcctt ttgggttaaa ccatataacg agatttccaa ttcatcacta tatgagttta
    12541 tggaaaagga caaagacggg tcttcaatcc acaatgtatt cagagcggat atcaccttgc
    12601 ttctctcttc gggcctgtct ggccggacgg aggatttgag agcgggatgc tgatgcgata
    12661 atccttgaat caaacaaggt tcagcaccta aataatttcc gattcgaaaa tcatccatat
    12721 cctctacaat cgcgatatca ttggcgccca cttcatcaac atttatctct ctgccctgat
    12781 tgatagtttt tagattttta atcttgatga atttttccga atcgttgatt cttacaacgt
    12841 ctcgaagtct cagacttccg tcaattattt ttagaaaact tcttttatgt cctttggggt
    12901 catgctctat cttataaaga taagatgaaa gtctgtttga gaccgatgcc ggaggaagta
    12961 taaaagaagt gatggcgtcc aacaactcat tgataccgat attgaacatt gctgatccat
    13021 gtagcaccgg atagactttg gcttttgcca caagagcgat tatcgtattc caataatcag
    13081 ccggtgaaat ttcgctatcc gccaaatatc gttctaatat attgtcgtca tggttgcata
    13141 caaattcttt gtattcttcc tttatatatg tttgggagca aaccggataa accgatccat
    13201 cgacaacatt ttgcataaac aggacatctt gagacagatt tgcttttata tccagataca
    13261 aacgctccaa attcacaccg gctcggtcaa tcttattgat aaatataatt gtcgggattt
    13321 gcagcttctg taaagtattg aacagcaact ttgtctgcgc ttgtatgcct tcctttgcgg
    13381 ataagatgag gactgctcca tcaagcattt tgaatgtccg ctccacttcc gcaataaaat
    13441 ccatgtgtcc cggagtgtca atgatattgc atttcacacc attccagata atagatgtcg
    13501 tagaagcccg aacagtaatt cctctacgtt tctctatatc catagagtcc gttatggtgt
    13561 caccattatc cacacagccg cacttttccg ttgctccact ggcaaacagc agattctcgg
    13621 ttacggaagt ttttcctgca tcaatgtgag caagaattcc taaatttata atattcattt
    13681 ggattaagca ataatatact acaatagatg cattgtcgaa acgcaccttt taatacctcc
    13741 tcgtagcata tgagaactac aggattccta actcgtatta atatgtatat tatt
//
