LOCUS       sequence-370301-       14094 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pBolux harboring the promoter region upstream of BT1763.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14094)
  AUTHORS   Modesto JL, Pearce VH, Townsend GE 2nd
  TITLE     Harnessing gut microbes for glycan detection and quantification.
  JOURNAL   Nat Commun. 2023 Jan 17;14(1):275. doi: 10.1038/s41467-022-35626-2.
  PUBMED    36650134
REFERENCE   2  (bases 1 to 14094)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-01-17"; volume: "14(1):275. doi"; pages: " 
            10.1038/s41467-022-35626-2"
COMMENT     Sequence Label: P-BT1763
FEATURES             Location/Qualifiers
     source          1..14094
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     653..671
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(709..731)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     831..850
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3752..3774
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3766..3783
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3767..3783
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             4166..5608
                     /codon_start=1
                     /gene="luxC"
                     /product="LuxC fatty acid reductase"
                     /label=LuxC
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MTKKISFIINGQVEIFPEGDDLVQSINFGDNSVYLPILNDSHVKN
                     IIDCNGNNELRLHNIVNFLYTVGQRWKNEEYSRRRTYIRDLKKYMGYSEEMAKLEANWI
                     SMILCSKGGLYDVVENELGSRHIMDEWLPQDESYVRAFPKGKSVHLLAGNVPLSGIMSI
                     LRAILTKNQCIIKTSSTDPFTANALALSFIDVDPNHPITRSLSVIYWPHQGDTSLAKEI
                     MQHADVIVAWGGPDAINWAVEHAPSYADVIKFGSKKSLCIIDNPVDLTSAATGAAHDVC
                     FYDQRACFSAQNIYYMGNHYEEFKLALIEKLNLYAHILPNAKKDFDEKAAYSLVQKESL
                     FAGLKVEVDIHQRWMIIESNAGVEFNQPLGRCVYLHHVDNIEQILPYVQKNKTQTISIF
                     PWESSFKYRDALALKGAERIVEAGMNNIFRVGGSHDGMRPLQRLVTYISHERPSNYTAK
                     DVAVEIEQTRFLEEDKFLVFVP"
     CDS             7797..8876
                     /codon_start=1
                     /gene="luxA"
                     /product="LuxA luciferase subunit"
                     /label=LuxA
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MKFGNFLLTYQPPQFSQTEVMKRLVKLGRISEECGFDTVWLLEHH
                     FTEFGLLGNPYVAAAYLLGATKKLNVGTAAIVLPTAHPVRQLEDVNLLDQMSKGRFRFG
                     ICRGLYNKDFRVFGTDMNNSRALAECWYGLIKNGMTEGYMEADNEHIKFHKVKVNPAAY
                     SRGGAPVYVVAESASTTEWAAQFGLPMILSWIINTNEKKAQLELYNEVAQEYGHDIHNI
                     DHCLSYITSVDHDSIKAKEICRKFLGHWYDSYVNATTIFDDSDQTRGYDFNKGQWRDFV
                     LKGHKDTNRRIDYSYEINPVGTPQECIDIIQKDIDATGISNICCGFEANGTVDEIIASM
                     KLFQSDVMPFLKEKQRSLL"
     primer_bind     complement(9910..9926)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9910..9926)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9923..9945)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9934..9950
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9958..9964,9965..9982,9983..9988))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10003..10024
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10141..10158)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10312..10900)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10392..10411)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11071..11862,11863..11931))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11694..11713
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11932..12036)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 atgtgagcaa gaattcctaa atttataata ttcatttgga ttaagcaata atatactaca
       61 atagatgcat tgtcgaaacg caccttttaa tacctcctcg tagcatatga gaactacagg
      121 attcctaact cgtattaata tgtatattat tactgccgca taacgattac aaaattacac
      181 aaaaaaatat atctaacaaa agtaggagga ttttttaact ttttaccata gacattttat
      241 tagggaaacg taggttcaac tggtaagtac aaataagtag aaatgtttga acaacactgt
      301 tttaggaagt tgaaaaatca agtttctctc tcggtatagc gtttattttg gccaatatct
      361 tctttagttt ggcatttgag tatttcccat tcgtgttcta tttagctcct tattatgagt
      421 atgtagcaag ttgcttatca aattcagcct ctttgagggt caaatatggt tttgcaaatg
      481 gtgactgaca agacataaac aaggtcagca ggatttttta cataaatgga catgaatgta
      541 tataacgggg tttgagggcc aatggaacga aaacgtacgt taaggagata attcgttgtt
      601 tatatttaaa tttagctaag aaaccattat tatcatgaca ttaacctata aaaataggcg
      661 tatcacgagg ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat
      721 gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg
      781 tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg cttaactatg cggcatcaga
      841 gcagattgta ctgagagtgc accattcccc gaccgatgat ttttaagtga ctgattttgt
      901 gctgttttgg gggtatatta agaataaaag aaatagaata agttaagtac ttgatacaca
      961 atatagggca ttttccatat tggaaattct cattttccaa tctagaaaat accgatttcc
     1021 taatatgata ctaaatagga aaataatatt tcccttaata ttgtttttat ggaaaataat
     1081 agtttacttt gtggagaata atatttccca aaaacatatc aaaatggaaa ataaaaaagc
     1141 agttaagtta accgattttc aaaagaacga agaaaatcct tttatgaaac aagctataga
     1201 aggtattgaa aatcatgttg ttaaaaagta taagagtaat agtggtggcg ataagagagc
     1261 tgtagtagct ttagccgaca ctgaaactgg agaagtgttt aagacttcgt ttatccgtca
     1321 aatagaagta gatgaagaac aattcactaa attgtatctt tctaactttg ctgcattctt
     1381 tgacctatca caagcagcta ttcgggtttt tggttacttt atgacctgca tgaaacccaa
     1441 aaatgattta atcattttca atagaaaaaa atgcctagaa tataccaaat acaaaacaga
     1501 caaagccgtt tataaaggac ttgcagaact tgtaaaagct gaaatcatag cccgaggacc
     1561 agccgataat ctttggttta ttaatcctct gatagtattc aatggtgacc gagtgacatt
     1621 tgctaaaaca tacgttcgga aaaagacttt agctgcccaa aagaaagaag aagcagagaa
     1681 acgacaatta tcacttggct ttgatgaaca gtaacactcc attgagtgaa gctgccgttt
     1741 ggtcgctccc cctttggggg cgggggggga tagataaagt tcctctatgt aaagttataa
     1801 tgggggatga aaggcaaggt tcgctaacct taccccgatt gtttatgctt cccagcattc
     1861 aatctatccc cgtttatggg tttctctttc accctacaaa gataacctca tgggggaaaa
     1921 atgtccaaga atatggggta aaaactatca agtcggtaga aaataagtat ctttgaagta
     1981 catttttagt agaggtactg gtgtgcctag gcgaagcaga taggcaaaaa taaagccgct
     2041 ggagatgtcg gatgtccgtc aattaactcc agcggcttgt attttggtaa ctctcccgta
     2101 tcggtcagct accgtttggt cagcaacaaa ggtagtactt tattatcgca aatccaaatg
     2161 tttttccctt tatttgtgca tttacaaaac attcactaac ttcgcaccta tgcagtatgg
     2221 tgatagcaca ctagcaccct aaagagtgcg tgtgtaaggg gacaagcccc cttaaccccc
     2281 tgtcagccgg ctgtcaccgg ctgttatcga ttgtcacaaa aatgttaact tatgggagca
     2341 acaagtattc atgtacaagc agtgaagccg gggagcgaga ttcacaactt tagggaaaaa
     2401 gagttggact atgttcgtcc cgaacttagt catttgaatg aaagctgggt tggagatagc
     2461 atttcccatc ggctggagag tgcaaaacaa agatatctcg atacggttgg gcagaagatg
     2521 caggctaaag ccgcacccat acgagaggga gtaatagtaa tcaaacaaga aaccactatg
     2581 caggaactcc agcagtttgc cacggtctgc aaagaacgtt tcggtatcga agcgtttcaa
     2641 atccatatac acaaggacga aggatacatg aacgcaaagc agtggacacc taaccttcat
     2701 gcccatgtag ttttcgattg gacgcagccg aatgggaaaa gtgtgcgttt atcgcgtgat
     2761 gacatggcag aactccaaac catagcatct gaaacactgg gcatggaacg tggcgtttct
     2821 tctgaccgca aacacttatc ggctatgcag tacaagaccg aatgtgcgaa agaacagctt
     2881 caggaactat ccaacgatat atccagtgca ttagacaaat acaaggacgc acaaaaccag
     2941 cttcttcagc ttcagaagga actacgttcc attgaaacaa agaagaacgt ccaaaaactc
     3001 atttctaagg cttcagagaa gttttacggc ttgattggaa aaacagtcaa cgatagggaa
     3061 aaggatgcct taaaagccaa agtaaagact ttagaggggg aaaatgaaca actatccgat
     3121 agactgggaa aggctatact tgaaaaagag cgaaacagca caaaggcatt caaagccgag
     3181 aatgacaagg agtattatag acaacaaatg gacaacgcaa ggacaaccag taacctatta
     3241 aggacggaga accaaaaact aaaagccgaa accaaggagt tgaaaaaaga acttggtaag
     3301 atgaaagact tgttcaattc cgaacaactg gaggctttaa ggcatcattt cccaaacata
     3361 tcaaaagcta tggaagaggg gaaagaccta ttcaagcaaa tcaccagaag cagaggtttc
     3421 ggcatgggta tgtaaccccc ccccgccccc aaagggggag cgaccaaacg gcagcctctc
     3481 tcaatggagt gttacaccgt tcggattcag aaaacatttt gcagggttcg gaaaacggtt
     3541 gtgtgcttat tctaaaaaaa gtttatggtg tgtatgcggt gtgaaatacc gcacagatgc
     3601 gtaaggagaa aataccgcat caggcgccat tcgccattca ggctgcgcaa ctgttgggaa
     3661 gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg atgtgctgca
     3721 aggcgattaa gttgggtaac gccagggttt tcccagtcac gacgttgtaa aacgacggcc
     3781 agtgaattcg agctcggtac ccggggatcc tatcattcag ttttctgttg gttactttga
     3841 gtgattatat gcttgttata tgaataatat ccctttttac aaggacttaa attactatac
     3901 aaaggtagta ttttctatga ttgagagaga atataatccc gactttctta tgatggagaa
     3961 ttattcttat aaaaccctat aaaatagcgt tctatgcaac atatcttaca gcatttgaaa
     4021 catttttttc agtcggttga cttaaatcaa ccgtaatttg catcatcgaa aagagaaaat
     4081 tcgtactttt aaattaataa cattaaacta actagtcact cccgcatttt aaaataaaat
     4141 aaattattta tttaattaaa cgaatatgac taaaaaaatt tcattcatta ttaacggcca
     4201 ggttgaaatc tttcccgaag gtgatgattt agtgcaatcc attaattttg gtgataatag
     4261 tgtttacctg ccaatattga atgactctca tgtaaaaaac attattgatt gtaatggaaa
     4321 taacgaatta cggttgcata acattgtcaa ttttctctat acggtagggc aaagatggaa
     4381 aaatgaagaa tactcaagac gcaggacata cattcgtgac ttaaaaaaat atatgggata
     4441 ttcagaagaa atggctaagc tagaggccaa ttggatatct atgattttat gttctaaagg
     4501 cggcctttat gatgttgtag aaaatgaact tggttctcgc catatcatgg atgaatggct
     4561 acctcaggat gaaagttatg ttcgggcttt tccgaaaggt aaatctgtac atctgttggc
     4621 aggtaatgtt ccattatctg ggatcatgtc tatattacgc gcaattttaa ctaagaatca
     4681 gtgtattata aaaacatcgt caaccgatcc ttttaccgct aatgcattag cgttaagttt
     4741 tattgatgta gaccctaatc atccgataac gcgctcttta tctgttatat attggcccca
     4801 ccaaggtgat acatcactcg caaaagaaat tatgcaacat gcggatgtta ttgtcgcttg
     4861 gggagggcca gatgcgatta attgggcggt agagcatgcg ccatcttatg ctgatgtgat
     4921 taaatttggt tctaaaaaga gtctttgcat tatcgataat cctgttgatt tgacgtccgc
     4981 agcgacaggt gcggctcatg atgtttgttt ttacgatcag cgagcttgtt tttctgccca
     5041 aaacatatat tacatgggaa atcattatga ggaatttaag ttagcgttga tagaaaaact
     5101 taatctatat gcgcatatat taccgaatgc caaaaaagat tttgatgaaa aggcggccta
     5161 ttctttagtt caaaaagaaa gcttgtttgc tggattaaaa gtagaggtgg atattcatca
     5221 acgttggatg attattgagt caaatgcagg tgtggaattt aatcaaccac ttggcagatg
     5281 tgtgtacctt catcacgtcg ataatattga gcaaatattg ccttatgttc aaaaaaataa
     5341 gacgcaaacc atatctattt ttccttggga gtcatcattt aaatatcgag atgcgttagc
     5401 attaaaaggt gcggaaagga ttgtagaagc aggaatgaat aacatatttc gagttggtgg
     5461 atctcatgac ggaatgagac cgttgcaacg attagtgaca tatatttctc atgaaaggcc
     5521 atctaactat acggctaagg atgttgcggt tgaaatagaa cagactcgat tcctggaaga
     5581 agataagttc cttgtatttg tcccgtaatc cgcatggcaa attgtaaatt gtaaatcgta
     5641 aaatagtaat atattaatgg aaaataaatc caaatataaa accatcgacc acgttatttg
     5701 tgttgaagga aataaaaaaa ttcatgtttg ggaaacgctg ccagaagaaa acagcccaaa
     5761 gagaaagaat gccattatta ttgcgtctgg ttttgcccgc aggatggatc attttgctgg
     5821 tctggcggaa tatttatcgc ggaatggatt tcatgtgatc cgctatgatt cgcttcacca
     5881 cgttggattg agttcaggga caattgatga atttacaatg tctataggaa agcagagctt
     5941 gttagcagtg gttgattggt taactacacg aaaaataaat aacttcggta tgttggcttc
     6001 aagcttatct gcgcggatag cttatgcaag cctatctgaa atcaatgctt cgtttttaat
     6061 caccgcagtc ggtgttgtta acttaagata ttctcttgaa agagctttag ggtttgatta
     6121 tctcagtcta cccattaatg aattgccgaa taatctagat tttgaaggcc ataaattggg
     6181 tgctgaagtc tttgcgagag attgtcttga ttttggttgg gaagatttag cttctacaat
     6241 taataacatg atgtatcttg atataccgtt tattgctttt actgcaaata acgataattg
     6301 ggtcaagcaa gatgaagtta tcacattgtt atcaaatatt cgtagtaatc gatgcaagat
     6361 atattctttg ttaggaagtt cgcatgactt gagtgaaaat ttagtggtcc tgcgcaattt
     6421 ttatcaatcg gttacgaaag ccgctatcgc gatggataat gatcatctgg atattgatgt
     6481 tgatattact gaaccgtcat ttgaacattt aactattgcg acagtcaatg aacgccgaat
     6541 gagaattgag attgaaaatc aagcgatttc gctgtcttaa taaggaggag gataaagaat
     6601 atgacttcat atgttgataa acaagaaatt acagcaagct cagaaattga tgatttgatt
     6661 ttttcgagcg atccattagt gtggtcttac gacgagcagg aaaaaatcag aaagaaactt
     6721 gtgcttgatg catttcgtaa tcattataaa cattgtcgag aatatcgtca ctactgtcag
     6781 gcacacaaag tagatgacaa tattacggaa attgatgaca tacctgtatt cccaacatcg
     6841 gtttttaagt ttactcgctt attaacttct caggaaaacg agattgaaag ttggtttacc
     6901 agtagcggca cgaatggttt aaaaagtcag gtggcgcgtg acagattaag tattgagaga
     6961 ctcttaggct ctgtgagtta tggcatgaaa tatgttggta gttggtttga tcatcaaata
     7021 gaattagtca atttgggacc agatagattt aatgctcata atatttggtt taaatatgtt
     7081 atgagtttgg tggaattgtt atatcctacg acatttaccg taacagaaga acgaatagat
     7141 tttgttaaaa cattgaatag tcttgaacga ataaaaaatc aagggaaaga tctttgtctt
     7201 attggttcgc catactttat ttatttactc tgccattata tgaaagataa aaaaatctca
     7261 ttttctggag ataaaagcct ttatatcata accggaggcg gctggaaaag ttacgaaaaa
     7321 gaatctctga aacgtgatga tttcaatcat cttttatttg atactttcaa tctcagtgat
     7381 attagtcaga tccgagatat atttaatcaa gttgaactca acacttgttt ctttgaggat
     7441 gaaatgcagc gtaaacatgt tccgccgtgg gtatatgcgc gagcgcttga tcctgaaacg
     7501 ttgaaacctg tacctgatgg aacgccgggg ttgatgagtt atatggatgc gtcagcaacc
     7561 agttatccag catttattgt taccgatgat gtcgggataa ttagcagaga atatggtaag
     7621 tatcccggcg tgctcgttga aattttacgt cgcgtcaata cgaggacgca gaaagggtgt
     7681 gctttaagct taaaccaagc atttaatagt tgatataaat gaagggtgaa gaaggatgaa
     7741 tgaaggtaac actcataaac tcgaaattct tcattcttaa tttttaatta aaatatatga
     7801 aatttggaaa ctttttgctt acataccaac ctccccaatt ttctcaaaca gaggtaatga
     7861 aacgtttggt taaattaggt cgcatctctg aggagtgtgg ttttgatacc gtatggttac
     7921 tggagcatca tttcacggag tttggtttgc ttggtaaccc ttatgtcgct gctgcatatt
     7981 tacttggcgc gactaaaaaa ttgaatgtag gaactgccgc tattgttctt cccacagccc
     8041 atccagtacg ccaacttgaa gatgtgaatt tattggatca aatgtcaaaa ggacgatttc
     8101 ggtttggtat ttgccgaggg ctttacaaca aggactttcg cgtattcggc acagatatga
     8161 ataacagtcg cgccttagcg gaatgctggt acgggctgat aaagaatggc atgacagagg
     8221 gatatatgga agctgataat gaacatatca agttccataa ggtaaaagta aaccccgcgg
     8281 cgtatagcag aggtggcgca ccggtttatg tggtggctga atcagcttcg acgactgagt
     8341 gggctgctca atttggccta ccgatgatat taagttggat tataaatact aacgaaaaga
     8401 aagcacaact tgagctttat aatgaagtgg ctcaagaata tgggcacgat attcataata
     8461 tcgaccattg cttatcatat ataacatctg tagatcatga ctcaattaaa gcgaaagaga
     8521 tttgccggaa atttctgggg cattggtatg attcttatgt gaatgctacg actatttttg
     8581 atgattcaga ccaaacaaga ggttatgatt tcaataaagg gcagtggcgt gactttgtat
     8641 taaaaggaca taaagatact aatcgccgta ttgattacag ttacgaaatc aatcccgtgg
     8701 gaacgccgca ggaatgtatt gacataattc aaaaagacat tgatgctaca ggaatatcaa
     8761 atatttgttg tggatttgaa gctaatggaa cagtagacga aattattgct tccatgaagc
     8821 tcttccagtc tgatgtcatg ccatttctta aagaaaaaca acgttcgcta ttataaagaa
     8881 aggaataaga caatggatat gaaatttgga ttgttcttcc ttaacttcat caattcaaca
     8941 actgttcaag aacaaagtat agttcgcatg caggaaataa cggagtatgt tgataagttg
     9001 aattttgaac agattttagt gtatgaaaat catttttcag ataatggtgt tgtcggcgct
     9061 cctctgactg tttctggttt tctgctcggt ttaacagaga aaattaaaat tggttcatta
     9121 aatcacatca ttacaactca tcatcctgtc cgcatagcgg aggaagcttg cttattggat
     9181 cagttaagtg aagggagatt tattttaggg tttagtgatt gcgaaaaaaa agatgaaatg
     9241 cattttttta atcgcccggt tgaatatcaa cagcaactat ttgaagagtg ttatgaaatc
     9301 attaacgatg ctttaacaac aggctattgt aatccagata acgattttta tagcttccct
     9361 aaaatatctg taaatcccca tgcttatacg ccaggcggac ctcggaaata tgtaacagca
     9421 accagtcatc atattgttga gtgggcggcc aaaaaaggta ttcctctcat ctttaagtgg
     9481 gatgattcta atgatgttag atatgaatat gctgaaagat ataaagccgt tgcggataaa
     9541 tatgacgttg acctatcaga gatagaccat cagttaatga tattagttaa ctataacgaa
     9601 gatagtaata aagctaaaca agagacgcgt gcatttatta gtgattatgt tcttgaaatg
     9661 caccctaatg aaaatttcga aaataaactt gaagaaataa ttgcagaaaa cgctgtcgga
     9721 aattatacgg agtgtataac tgcggctaag ttggcaattg aaaagtgtgg tgcgaaaagt
     9781 gtattgctgt cctttgaacc aatgaatgat ttgatgagcc aaaaaaatgt aatcaatatt
     9841 gttgatgata atattaaaaa gtaccacatg taagtcgacc tgcaggcatg caagcttggc
     9901 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
     9961 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
    10021 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
    10081 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
    10141 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
    10201 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
    10261 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
    10321 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
    10381 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
    10441 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
    10501 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
    10561 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
    10621 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
    10681 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
    10741 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
    10801 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
    10861 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
    10921 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
    10981 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
    11041 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
    11101 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
    11161 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
    11221 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
    11281 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
    11341 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
    11401 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
    11461 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
    11521 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
    11581 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
    11641 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
    11701 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
    11761 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
    11821 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
    11881 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
    11941 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
    12001 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
    12061 tgcgtctatt tttttattgc caagttctaa tgcttctatc tcctgccatt catagaggct
    12121 attatatgcc atacatccga taagaatgga gataagtata tatcccaact gtattgcctt
    12181 atagaaattt cctgaccgct ccattatttt gatgacattg atttttggaa catgaataaa
    12241 agtttatctt tttcgttcat gcggatatta tcagaataac cgccttttgt tatttgatac
    12301 ccgcatggct taaccataaa aatgcctaag cccttagtgt atgaacttac ttctgatgca
    12361 tagtctttac ttgtatttaa tggaactttc cctttaatat gacaccactc attattgcaa
    12421 ctgatgtctt caatctcaga catcattttt tgcaaatctg taatagcttt ggaacttgcc
    12481 gcttggggta tctgcaactc aaaatagagc atcggttcga gaatgtccac acctgactgt
    12541 tgcaaggcca gcctgaagac ataaggggtc agctgtctga aatcagcagg tgtacttacc
    12601 gggctataat actcggcttg agtaaaagtt actttcagat cagtcacttc ccatccatgt
    12661 aacccggatt ggcaagacat acgaatccct tcaaaaacgg cattttgaaa agaatggttc
    12721 agataaccat aggagatgtc actttcgatt tgcaaccctg tccctaacgg taagggttca
    12781 agagtcagcc ctattgtggc ccaataaggg ttgggcggca cttcgatctg aataatctta
    12841 ttgacctttt ttacaggtcg ttctttgtat atagtcttga tctcatcaaa atggaccttt
    12901 acggaaaatc gttcttccag caatgtctgt atgatttcct tttgggttaa accatataac
    12961 gagatttcca attcatcact atatgagttt atggaaaagg acaaagacgg gtcttcaatc
    13021 cacaatgtat tcagagcgga tatcaccttg cttctctctt cgggcctgtc tggccggacg
    13081 gaggatttga gagcgggatg ctgatgcgat aatccttgaa tcaaacaagg ttcagcacct
    13141 aaataatttc cgattcgaaa atcatccata tcctctacaa tcgcgatatc attggcgccc
    13201 acttcatcaa catttatctc tctgccctga ttgatagttt ttagattttt aatcttgatg
    13261 aatttttccg aatcgttgat tcttacaacg tctcgaagtc tcagacttcc gtcaattatt
    13321 tttagaaaac ttcttttatg tcctttgggg tcatgctcta tcttataaag ataagatgaa
    13381 agtctgtttg agaccgatgc cggaggaagt ataaaagaag tgatggcgtc caacaactca
    13441 ttgataccga tattgaacat tgctgatcca tgtagcaccg gatagacttt ggcttttgcc
    13501 acaagagcga ttatcgtatt ccaataatca gccggtgaaa tttcgctatc cgccaaatat
    13561 cgttctaata tattgtcgtc atggttgcat acaaattctt tgtattcttc ctttatatat
    13621 gtttgggagc aaaccggata aaccgatcca tcgacaacat tttgcataaa caggacatct
    13681 tgagacagat ttgcttttat atccagatac aaacgctcca aattcacacc ggctcggtca
    13741 atcttattga taaatataat tgtcgggatt tgcagcttct gtaaagtatt gaacagcaac
    13801 tttgtctgcg cttgtatgcc ttcctttgcg gataagatga ggactgctcc atcaagcatt
    13861 ttgaatgtcc gctccacttc cgcaataaaa tccatgtgtc ccggagtgtc aatgatattg
    13921 catttcacac cattccagat aatagatgtc gtagaagccc gaacagtaat tcctctacgt
    13981 ttctctatat ccatagagtc cgttatggtg tcaccattat ccacacagcc gcacttttcc
    14041 gttgctccac tggcaaacag cagattctcg gttacggaag tttttcctgc atca
//
