LOCUS       sequence-370302-       14094 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pBolux harboring the promoter region upstream of BACOVA_04505.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14094)
  AUTHORS   Modesto JL, Pearce VH, Townsend GE 2nd
  TITLE     Harnessing gut microbes for glycan detection and quantification.
  JOURNAL   Nat Commun. 2023 Jan 17;14(1):275. doi: 10.1038/s41467-022-35626-2.
  PUBMED    36650134
REFERENCE   2  (bases 1 to 14094)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-01-17"; volume: "14(1):275. doi"; pages: " 
            10.1038/s41467-022-35626-2"
COMMENT     Sequence Label: P-BACOVA_04505
FEATURES             Location/Qualifiers
     source          1..14094
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     564..582
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(620..642)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     742..761
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3663..3685
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3677..3694
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3678..3694
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             4077..5519
                     /codon_start=1
                     /gene="luxC"
                     /product="LuxC fatty acid reductase"
                     /label=LuxC
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MTKKISFIINGQVEIFPEGDDLVQSINFGDNSVYLPILNDSHVKN
                     IIDCNGNNELRLHNIVNFLYTVGQRWKNEEYSRRRTYIRDLKKYMGYSEEMAKLEANWI
                     SMILCSKGGLYDVVENELGSRHIMDEWLPQDESYVRAFPKGKSVHLLAGNVPLSGIMSI
                     LRAILTKNQCIIKTSSTDPFTANALALSFIDVDPNHPITRSLSVIYWPHQGDTSLAKEI
                     MQHADVIVAWGGPDAINWAVEHAPSYADVIKFGSKKSLCIIDNPVDLTSAATGAAHDVC
                     FYDQRACFSAQNIYYMGNHYEEFKLALIEKLNLYAHILPNAKKDFDEKAAYSLVQKESL
                     FAGLKVEVDIHQRWMIIESNAGVEFNQPLGRCVYLHHVDNIEQILPYVQKNKTQTISIF
                     PWESSFKYRDALALKGAERIVEAGMNNIFRVGGSHDGMRPLQRLVTYISHERPSNYTAK
                     DVAVEIEQTRFLEEDKFLVFVP"
     CDS             7708..8787
                     /codon_start=1
                     /gene="luxA"
                     /product="LuxA luciferase subunit"
                     /label=LuxA
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MKFGNFLLTYQPPQFSQTEVMKRLVKLGRISEECGFDTVWLLEHH
                     FTEFGLLGNPYVAAAYLLGATKKLNVGTAAIVLPTAHPVRQLEDVNLLDQMSKGRFRFG
                     ICRGLYNKDFRVFGTDMNNSRALAECWYGLIKNGMTEGYMEADNEHIKFHKVKVNPAAY
                     SRGGAPVYVVAESASTTEWAAQFGLPMILSWIINTNEKKAQLELYNEVAQEYGHDIHNI
                     DHCLSYITSVDHDSIKAKEICRKFLGHWYDSYVNATTIFDDSDQTRGYDFNKGQWRDFV
                     LKGHKDTNRRIDYSYEINPVGTPQECIDIIQKDIDATGISNICCGFEANGTVDEIIASM
                     KLFQSDVMPFLKEKQRSLL"
     primer_bind     complement(9821..9837)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9821..9837)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9834..9856)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9845..9861
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9869..9875,9876..9893,9894..9899))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9914..9935
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10052..10069)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10223..10811)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10303..10322)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10982..11773,11774..11842))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11605..11624
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11843..11947)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 atacctcctc gtagcatatg agaactacag gattcctaac tcgtattaat atgtatatta
       61 ttactgccgc ataacgatta caaaattaca caaaaaaata tatctaacaa aagtaggagg
      121 attttttaac tttttaccat agacatttta ttagggaaac gtaggttcaa ctggtaagta
      181 caaataagta gaaatgtttg aacaacactg ttttaggaag ttgaaaaatc aagtttctct
      241 ctcggtatag cgtttatttt ggccaatatc ttctttagtt tggcatttga gtatttccca
      301 ttcgtgttct atttagctcc ttattatgag tatgtagcaa gttgcttatc aaattcagcc
      361 tctttgaggg tcaaatatgg ttttgcaaat ggtgactgac aagacataaa caaggtcagc
      421 aggatttttt acataaatgg acatgaatgt atataacggg gtttgagggc caatggaacg
      481 aaaacgtacg ttaaggagat aattcgttgt ttatatttaa atttagctaa gaaaccatta
      541 ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt ctcgcgcgtt
      601 tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc
      661 tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt
      721 gtcggggctg gcttaactat gcggcatcag agcagattgt actgagagtg caccattccc
      781 cgaccgatga tttttaagtg actgattttg tgctgttttg ggggtatatt aagaataaaa
      841 gaaatagaat aagttaagta cttgatacac aatatagggc attttccata ttggaaattc
      901 tcattttcca atctagaaaa taccgatttc ctaatatgat actaaatagg aaaataatat
      961 ttcccttaat attgttttta tggaaaataa tagtttactt tgtggagaat aatatttccc
     1021 aaaaacatat caaaatggaa aataaaaaag cagttaagtt aaccgatttt caaaagaacg
     1081 aagaaaatcc ttttatgaaa caagctatag aaggtattga aaatcatgtt gttaaaaagt
     1141 ataagagtaa tagtggtggc gataagagag ctgtagtagc tttagccgac actgaaactg
     1201 gagaagtgtt taagacttcg tttatccgtc aaatagaagt agatgaagaa caattcacta
     1261 aattgtatct ttctaacttt gctgcattct ttgacctatc acaagcagct attcgggttt
     1321 ttggttactt tatgacctgc atgaaaccca aaaatgattt aatcattttc aatagaaaaa
     1381 aatgcctaga atataccaaa tacaaaacag acaaagccgt ttataaagga cttgcagaac
     1441 ttgtaaaagc tgaaatcata gcccgaggac cagccgataa tctttggttt attaatcctc
     1501 tgatagtatt caatggtgac cgagtgacat ttgctaaaac atacgttcgg aaaaagactt
     1561 tagctgccca aaagaaagaa gaagcagaga aacgacaatt atcacttggc tttgatgaac
     1621 agtaacactc cattgagtga agctgccgtt tggtcgctcc ccctttgggg gcgggggggg
     1681 atagataaag ttcctctatg taaagttata atgggggatg aaaggcaagg ttcgctaacc
     1741 ttaccccgat tgtttatgct tcccagcatt caatctatcc ccgtttatgg gtttctcttt
     1801 caccctacaa agataacctc atgggggaaa aatgtccaag aatatggggt aaaaactatc
     1861 aagtcggtag aaaataagta tctttgaagt acatttttag tagaggtact ggtgtgccta
     1921 ggcgaagcag ataggcaaaa ataaagccgc tggagatgtc ggatgtccgt caattaactc
     1981 cagcggcttg tattttggta actctcccgt atcggtcagc taccgtttgg tcagcaacaa
     2041 aggtagtact ttattatcgc aaatccaaat gtttttccct ttatttgtgc atttacaaaa
     2101 cattcactaa cttcgcacct atgcagtatg gtgatagcac actagcaccc taaagagtgc
     2161 gtgtgtaagg ggacaagccc ccttaacccc ctgtcagccg gctgtcaccg gctgttatcg
     2221 attgtcacaa aaatgttaac ttatgggagc aacaagtatt catgtacaag cagtgaagcc
     2281 ggggagcgag attcacaact ttagggaaaa agagttggac tatgttcgtc ccgaacttag
     2341 tcatttgaat gaaagctggg ttggagatag catttcccat cggctggaga gtgcaaaaca
     2401 aagatatctc gatacggttg ggcagaagat gcaggctaaa gccgcaccca tacgagaggg
     2461 agtaatagta atcaaacaag aaaccactat gcaggaactc cagcagtttg ccacggtctg
     2521 caaagaacgt ttcggtatcg aagcgtttca aatccatata cacaaggacg aaggatacat
     2581 gaacgcaaag cagtggacac ctaaccttca tgcccatgta gttttcgatt ggacgcagcc
     2641 gaatgggaaa agtgtgcgtt tatcgcgtga tgacatggca gaactccaaa ccatagcatc
     2701 tgaaacactg ggcatggaac gtggcgtttc ttctgaccgc aaacacttat cggctatgca
     2761 gtacaagacc gaatgtgcga aagaacagct tcaggaacta tccaacgata tatccagtgc
     2821 attagacaaa tacaaggacg cacaaaacca gcttcttcag cttcagaagg aactacgttc
     2881 cattgaaaca aagaagaacg tccaaaaact catttctaag gcttcagaga agttttacgg
     2941 cttgattgga aaaacagtca acgataggga aaaggatgcc ttaaaagcca aagtaaagac
     3001 tttagagggg gaaaatgaac aactatccga tagactggga aaggctatac ttgaaaaaga
     3061 gcgaaacagc acaaaggcat tcaaagccga gaatgacaag gagtattata gacaacaaat
     3121 ggacaacgca aggacaacca gtaacctatt aaggacggag aaccaaaaac taaaagccga
     3181 aaccaaggag ttgaaaaaag aacttggtaa gatgaaagac ttgttcaatt ccgaacaact
     3241 ggaggcttta aggcatcatt tcccaaacat atcaaaagct atggaagagg ggaaagacct
     3301 attcaagcaa atcaccagaa gcagaggttt cggcatgggt atgtaacccc cccccgcccc
     3361 caaaggggga gcgaccaaac ggcagcctct ctcaatggag tgttacaccg ttcggattca
     3421 gaaaacattt tgcagggttc ggaaaacggt tgtgtgctta ttctaaaaaa agtttatggt
     3481 gtgtatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggcgcca
     3541 ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt
     3601 acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt
     3661 ttcccagtca cgacgttgta aaacgacggc cagtgaattc gagctcggta cccggggatc
     3721 cttgtttgtt ggagattgtt ttcatatcgt tgattttttc agttgactac aaccagtagt
     3781 taactctatt tttaagttct tatcacctgc aaaggtaata ttttccatac ttactggaga
     3841 atagatattc tataattctt ctatttcgaa gaataatgta taaaacccta taaaatagcg
     3901 ttctatgcaa caaatgttat agcctttgaa acattttttt cagtcgtttg atataaatca
     3961 accgtaattt gcatcatcga tatagtaaaa ttcgtacttt taatttaata acatcaaact
     4021 aactagtcac tcccgcattt taaaataaaa taaattattt atttaattaa acgaatatga
     4081 ctaaaaaaat ttcattcatt attaacggcc aggttgaaat ctttcccgaa ggtgatgatt
     4141 tagtgcaatc cattaatttt ggtgataata gtgtttacct gccaatattg aatgactctc
     4201 atgtaaaaaa cattattgat tgtaatggaa ataacgaatt acggttgcat aacattgtca
     4261 attttctcta tacggtaggg caaagatgga aaaatgaaga atactcaaga cgcaggacat
     4321 acattcgtga cttaaaaaaa tatatgggat attcagaaga aatggctaag ctagaggcca
     4381 attggatatc tatgatttta tgttctaaag gcggccttta tgatgttgta gaaaatgaac
     4441 ttggttctcg ccatatcatg gatgaatggc tacctcagga tgaaagttat gttcgggctt
     4501 ttccgaaagg taaatctgta catctgttgg caggtaatgt tccattatct gggatcatgt
     4561 ctatattacg cgcaatttta actaagaatc agtgtattat aaaaacatcg tcaaccgatc
     4621 cttttaccgc taatgcatta gcgttaagtt ttattgatgt agaccctaat catccgataa
     4681 cgcgctcttt atctgttata tattggcccc accaaggtga tacatcactc gcaaaagaaa
     4741 ttatgcaaca tgcggatgtt attgtcgctt ggggagggcc agatgcgatt aattgggcgg
     4801 tagagcatgc gccatcttat gctgatgtga ttaaatttgg ttctaaaaag agtctttgca
     4861 ttatcgataa tcctgttgat ttgacgtccg cagcgacagg tgcggctcat gatgtttgtt
     4921 tttacgatca gcgagcttgt ttttctgccc aaaacatata ttacatggga aatcattatg
     4981 aggaatttaa gttagcgttg atagaaaaac ttaatctata tgcgcatata ttaccgaatg
     5041 ccaaaaaaga ttttgatgaa aaggcggcct attctttagt tcaaaaagaa agcttgtttg
     5101 ctggattaaa agtagaggtg gatattcatc aacgttggat gattattgag tcaaatgcag
     5161 gtgtggaatt taatcaacca cttggcagat gtgtgtacct tcatcacgtc gataatattg
     5221 agcaaatatt gccttatgtt caaaaaaata agacgcaaac catatctatt tttccttggg
     5281 agtcatcatt taaatatcga gatgcgttag cattaaaagg tgcggaaagg attgtagaag
     5341 caggaatgaa taacatattt cgagttggtg gatctcatga cggaatgaga ccgttgcaac
     5401 gattagtgac atatatttct catgaaaggc catctaacta tacggctaag gatgttgcgg
     5461 ttgaaataga acagactcga ttcctggaag aagataagtt ccttgtattt gtcccgtaat
     5521 ccgcatggca aattgtaaat tgtaaatcgt aaaatagtaa tatattaatg gaaaataaat
     5581 ccaaatataa aaccatcgac cacgttattt gtgttgaagg aaataaaaaa attcatgttt
     5641 gggaaacgct gccagaagaa aacagcccaa agagaaagaa tgccattatt attgcgtctg
     5701 gttttgcccg caggatggat cattttgctg gtctggcgga atatttatcg cggaatggat
     5761 ttcatgtgat ccgctatgat tcgcttcacc acgttggatt gagttcaggg acaattgatg
     5821 aatttacaat gtctatagga aagcagagct tgttagcagt ggttgattgg ttaactacac
     5881 gaaaaataaa taacttcggt atgttggctt caagcttatc tgcgcggata gcttatgcaa
     5941 gcctatctga aatcaatgct tcgtttttaa tcaccgcagt cggtgttgtt aacttaagat
     6001 attctcttga aagagcttta gggtttgatt atctcagtct acccattaat gaattgccga
     6061 ataatctaga ttttgaaggc cataaattgg gtgctgaagt ctttgcgaga gattgtcttg
     6121 attttggttg ggaagattta gcttctacaa ttaataacat gatgtatctt gatataccgt
     6181 ttattgcttt tactgcaaat aacgataatt gggtcaagca agatgaagtt atcacattgt
     6241 tatcaaatat tcgtagtaat cgatgcaaga tatattcttt gttaggaagt tcgcatgact
     6301 tgagtgaaaa tttagtggtc ctgcgcaatt tttatcaatc ggttacgaaa gccgctatcg
     6361 cgatggataa tgatcatctg gatattgatg ttgatattac tgaaccgtca tttgaacatt
     6421 taactattgc gacagtcaat gaacgccgaa tgagaattga gattgaaaat caagcgattt
     6481 cgctgtctta ataaggagga ggataaagaa tatgacttca tatgttgata aacaagaaat
     6541 tacagcaagc tcagaaattg atgatttgat tttttcgagc gatccattag tgtggtctta
     6601 cgacgagcag gaaaaaatca gaaagaaact tgtgcttgat gcatttcgta atcattataa
     6661 acattgtcga gaatatcgtc actactgtca ggcacacaaa gtagatgaca atattacgga
     6721 aattgatgac atacctgtat tcccaacatc ggtttttaag tttactcgct tattaacttc
     6781 tcaggaaaac gagattgaaa gttggtttac cagtagcggc acgaatggtt taaaaagtca
     6841 ggtggcgcgt gacagattaa gtattgagag actcttaggc tctgtgagtt atggcatgaa
     6901 atatgttggt agttggtttg atcatcaaat agaattagtc aatttgggac cagatagatt
     6961 taatgctcat aatatttggt ttaaatatgt tatgagtttg gtggaattgt tatatcctac
     7021 gacatttacc gtaacagaag aacgaataga ttttgttaaa acattgaata gtcttgaacg
     7081 aataaaaaat caagggaaag atctttgtct tattggttcg ccatacttta tttatttact
     7141 ctgccattat atgaaagata aaaaaatctc attttctgga gataaaagcc tttatatcat
     7201 aaccggaggc ggctggaaaa gttacgaaaa agaatctctg aaacgtgatg atttcaatca
     7261 tcttttattt gatactttca atctcagtga tattagtcag atccgagata tatttaatca
     7321 agttgaactc aacacttgtt tctttgagga tgaaatgcag cgtaaacatg ttccgccgtg
     7381 ggtatatgcg cgagcgcttg atcctgaaac gttgaaacct gtacctgatg gaacgccggg
     7441 gttgatgagt tatatggatg cgtcagcaac cagttatcca gcatttattg ttaccgatga
     7501 tgtcgggata attagcagag aatatggtaa gtatcccggc gtgctcgttg aaattttacg
     7561 tcgcgtcaat acgaggacgc agaaagggtg tgctttaagc ttaaaccaag catttaatag
     7621 ttgatataaa tgaagggtga agaaggatga atgaaggtaa cactcataaa ctcgaaattc
     7681 ttcattctta atttttaatt aaaatatatg aaatttggaa actttttgct tacataccaa
     7741 cctccccaat tttctcaaac agaggtaatg aaacgtttgg ttaaattagg tcgcatctct
     7801 gaggagtgtg gttttgatac cgtatggtta ctggagcatc atttcacgga gtttggtttg
     7861 cttggtaacc cttatgtcgc tgctgcatat ttacttggcg cgactaaaaa attgaatgta
     7921 ggaactgccg ctattgttct tcccacagcc catccagtac gccaacttga agatgtgaat
     7981 ttattggatc aaatgtcaaa aggacgattt cggtttggta tttgccgagg gctttacaac
     8041 aaggactttc gcgtattcgg cacagatatg aataacagtc gcgccttagc ggaatgctgg
     8101 tacgggctga taaagaatgg catgacagag ggatatatgg aagctgataa tgaacatatc
     8161 aagttccata aggtaaaagt aaaccccgcg gcgtatagca gaggtggcgc accggtttat
     8221 gtggtggctg aatcagcttc gacgactgag tgggctgctc aatttggcct accgatgata
     8281 ttaagttgga ttataaatac taacgaaaag aaagcacaac ttgagcttta taatgaagtg
     8341 gctcaagaat atgggcacga tattcataat atcgaccatt gcttatcata tataacatct
     8401 gtagatcatg actcaattaa agcgaaagag atttgccgga aatttctggg gcattggtat
     8461 gattcttatg tgaatgctac gactattttt gatgattcag accaaacaag aggttatgat
     8521 ttcaataaag ggcagtggcg tgactttgta ttaaaaggac ataaagatac taatcgccgt
     8581 attgattaca gttacgaaat caatcccgtg ggaacgccgc aggaatgtat tgacataatt
     8641 caaaaagaca ttgatgctac aggaatatca aatatttgtt gtggatttga agctaatgga
     8701 acagtagacg aaattattgc ttccatgaag ctcttccagt ctgatgtcat gccatttctt
     8761 aaagaaaaac aacgttcgct attataaaga aaggaataag acaatggata tgaaatttgg
     8821 attgttcttc cttaacttca tcaattcaac aactgttcaa gaacaaagta tagttcgcat
     8881 gcaggaaata acggagtatg ttgataagtt gaattttgaa cagattttag tgtatgaaaa
     8941 tcatttttca gataatggtg ttgtcggcgc tcctctgact gtttctggtt ttctgctcgg
     9001 tttaacagag aaaattaaaa ttggttcatt aaatcacatc attacaactc atcatcctgt
     9061 ccgcatagcg gaggaagctt gcttattgga tcagttaagt gaagggagat ttattttagg
     9121 gtttagtgat tgcgaaaaaa aagatgaaat gcattttttt aatcgcccgg ttgaatatca
     9181 acagcaacta tttgaagagt gttatgaaat cattaacgat gctttaacaa caggctattg
     9241 taatccagat aacgattttt atagcttccc taaaatatct gtaaatcccc atgcttatac
     9301 gccaggcgga cctcggaaat atgtaacagc aaccagtcat catattgttg agtgggcggc
     9361 caaaaaaggt attcctctca tctttaagtg ggatgattct aatgatgtta gatatgaata
     9421 tgctgaaaga tataaagccg ttgcggataa atatgacgtt gacctatcag agatagacca
     9481 tcagttaatg atattagtta actataacga agatagtaat aaagctaaac aagagacgcg
     9541 tgcatttatt agtgattatg ttcttgaaat gcaccctaat gaaaatttcg aaaataaact
     9601 tgaagaaata attgcagaaa acgctgtcgg aaattatacg gagtgtataa ctgcggctaa
     9661 gttggcaatt gaaaagtgtg gtgcgaaaag tgtattgctg tcctttgaac caatgaatga
     9721 tttgatgagc caaaaaaatg taatcaatat tgttgatgat aatattaaaa agtaccacat
     9781 gtaagtcgac ctgcaggcat gcaagcttgg cgtaatcatg gtcatagctg tttcctgtgt
     9841 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag
     9901 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt
     9961 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag
    10021 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
    10081 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
    10141 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
    10201 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
    10261 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
    10321 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
    10381 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
    10441 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
    10501 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
    10561 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
    10621 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct
    10681 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
    10741 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
    10801 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
    10861 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
    10921 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
    10981 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
    11041 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
    11101 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
    11161 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
    11221 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
    11281 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
    11341 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
    11401 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
    11461 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
    11521 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
    11581 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
    11641 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
    11701 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
    11761 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
    11821 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
    11881 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
    11941 ttccgcgcac atttccccga aaagtgccac ctgcgtctat ttttttattg ccaagttcta
    12001 atgcttctat ctcctgccat tcatagaggc tattatatgc catacatccg ataagaatgg
    12061 agataagtat atatcccaac tgtattgcct tatagaaatt tcctgaccgc tccattattt
    12121 tgatgacatt gatttttgga acatgaataa aagtttatct ttttcgttca tgcggatatt
    12181 atcagaataa ccgccttttg ttatttgata cccgcatggc ttaaccataa aaatgcctaa
    12241 gcccttagtg tatgaactta cttctgatgc atagtcttta cttgtattta atggaacttt
    12301 ccctttaata tgacaccact cattattgca actgatgtct tcaatctcag acatcatttt
    12361 ttgcaaatct gtaatagctt tggaacttgc cgcttggggt atctgcaact caaaatagag
    12421 catcggttcg agaatgtcca cacctgactg ttgcaaggcc agcctgaaga cataaggggt
    12481 cagctgtctg aaatcagcag gtgtacttac cgggctataa tactcggctt gagtaaaagt
    12541 tactttcaga tcagtcactt cccatccatg taacccggat tggcaagaca tacgaatccc
    12601 ttcaaaaacg gcattttgaa aagaatggtt cagataacca taggagatgt cactttcgat
    12661 ttgcaaccct gtccctaacg gtaagggttc aagagtcagc cctattgtgg cccaataagg
    12721 gttgggcggc acttcgatct gaataatctt attgaccttt tttacaggtc gttctttgta
    12781 tatagtcttg atctcatcaa aatggacctt tacggaaaat cgttcttcca gcaatgtctg
    12841 tatgatttcc ttttgggtta aaccatataa cgagatttcc aattcatcac tatatgagtt
    12901 tatggaaaag gacaaagacg ggtcttcaat ccacaatgta ttcagagcgg atatcacctt
    12961 gcttctctct tcgggcctgt ctggccggac ggaggatttg agagcgggat gctgatgcga
    13021 taatccttga atcaaacaag gttcagcacc taaataattt ccgattcgaa aatcatccat
    13081 atcctctaca atcgcgatat cattggcgcc cacttcatca acatttatct ctctgccctg
    13141 attgatagtt tttagatttt taatcttgat gaatttttcc gaatcgttga ttcttacaac
    13201 gtctcgaagt ctcagacttc cgtcaattat ttttagaaaa cttcttttat gtcctttggg
    13261 gtcatgctct atcttataaa gataagatga aagtctgttt gagaccgatg ccggaggaag
    13321 tataaaagaa gtgatggcgt ccaacaactc attgataccg atattgaaca ttgctgatcc
    13381 atgtagcacc ggatagactt tggcttttgc cacaagagcg attatcgtat tccaataatc
    13441 agccggtgaa atttcgctat ccgccaaata tcgttctaat atattgtcgt catggttgca
    13501 tacaaattct ttgtattctt cctttatata tgtttgggag caaaccggat aaaccgatcc
    13561 atcgacaaca ttttgcataa acaggacatc ttgagacaga tttgctttta tatccagata
    13621 caaacgctcc aaattcacac cggctcggtc aatcttattg ataaatataa ttgtcgggat
    13681 ttgcagcttc tgtaaagtat tgaacagcaa ctttgtctgc gcttgtatgc cttcctttgc
    13741 ggataagatg aggactgctc catcaagcat tttgaatgtc cgctccactt ccgcaataaa
    13801 atccatgtgt cccggagtgt caatgatatt gcatttcaca ccattccaga taatagatgt
    13861 cgtagaagcc cgaacagtaa ttcctctacg tttctctata tccatagagt ccgttatggt
    13921 gtcaccatta tccacacagc cgcacttttc cgttgctcca ctggcaaaca gcagattctc
    13981 ggttacggaa gtttttcctg catcaatgtg agcaagaatt cctaaattta taatattcat
    14041 ttggattaag caataatata ctacaataga tgcattgtcg aaacgcacct ttta
//
