LOCUS       sequence-37033-e         710 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 710)
  AUTHORS   Thomas H, Senkel S, Erdmann S, Arndt T, Turan G, Klein-Hitpass L, 
            Ryffel GU
  TITLE     Pattern of genes influenced by conditional expression of the 
            transcription factors HNF6, HNF4alpha and HNF1beta in a pancreatic 
            beta-cell line.
  JOURNAL   Nucleic Acids Res. 2004  . 32(19):e150.
  PUBMED    15520459
REFERENCE   2  (bases 1 to 710)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2004  . 32(19):e150."
COMMENT     Sequence Label: FR_HNF6
FEATURES             Location/Qualifiers
     source          1..710
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    22..40
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    43..61
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     71..95
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             206..223
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 cggnggggtn tagcagagct ctccctatca gtgatagaga tctccctatc agtgatagag
       61 atcgtcgacg agctcgttta gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt
      121 ttgacctcca tagaagacac cgggaccgat ccagcctccg gactctagcg tttaaactta
      181 agctatcgat accatgcggg gttctcatca tcatcatcat catggtgaag ctttgataga
      241 catgaacgcg cagctgacca tggaagcgat cggcgagctg cacggggtga gccatgagcc
      301 ggtgcccgcc cctgccgacc tgctgggcgg cagcccccac gcgcgcagct ccgtggcgca
      361 ccgcggcagc cacctgcccc ccgcgcaccc gcgctccatg ggcatggcgt ccctgctgga
      421 cggcggcagc ggcggcggag attaccacca ccaccaccgg gcccctgagc acagcctggc
      481 cggncccctg natcccacca tgaccatggc ctgcnagact cccccaggta tgancatgcc
      541 caccacctac accatcttga cccctctgca gccgctgcct cccatctcca cagtctcgga
      601 caagttcccc cancatcacc accaccacna tcaccancac cacncnnacc accacnancg
      661 cntggcgggc aacgtgagcg gtagcttcac nctcatgcgg gatgagcgcg
//
