LOCUS       sequence-37034-e         502 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 502)
  AUTHORS   Zhu C, Smith T, McNulty J, Rayla AL, Lakshmanan A, Siekmann AF, 
            Buffardi M, Meng X, Shin J, Padmanabhan A, Cifuentes D, Giraldez AJ,
            Look AT, Epstein JA, Lawson ND, Wolfe SA
  TITLE     Evaluation and application of modularly assembled zinc-finger 
            nucleases in zebrafish.
  JOURNAL   Development. 2011 Oct;138(20):4555-64.
  PUBMED    21937602
REFERENCE   2  (bases 1 to 502)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Development."; date: "2011-10"; volume: "138(20)"; pages: "4555-64"
COMMENT     Sequence Label: pBS-F1-CGG
FEATURES             Location/Qualifiers
     source          1..502
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     8..24
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8..24
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(383..402)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(384..402)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(412..429)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(412..428)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(421..443)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 tttcacacag gaaacagcta tgaccatgat tacgccaagc gcgcaattaa cccttactaa
       61 agggaacaaa agctggagct ccaccgcggt ggcggccgct ctagattact tttcacctgt
      121 atggattttg gtatgcctct tgcgttcatc actcctggca aacttcctcc cacaaatgtc
      181 gcatgcaaaa ggcttctcgc ctgtgtgggt cctgatgtgg gtggtcaggt ggctggagcg
      241 actgaagtta cgcatgcaga tgcgacactg gaagggcttc tggccggtgt ggatccggat
      301 gtggtcagag aggtggtcag aacgactgaa gcggcggtca caggactcga cagggcaagc
      361 atatgggcgg gtacccaatt cgccctatag tgagtcgtat tacgcgcgct cactggccgt
      421 cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc
      481 acatccccct ttcgccagct gg
//
