LOCUS       sequence-370383-        5411 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  rpsM promoter driving the expression of destabilized GFPmut3 
            (containing a AAV C-terminal tail).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5411)
  AUTHORS   Ciolli Mattioli C, Eisner K, Rosenbaum A, Wang M, Rivalta A, Amir A,
            Golding I, Avraham R
  TITLE     Physiological stress drives the emergence of a Salmonella 
            subpopulation through ribosomal RNA regulation.
  JOURNAL   Curr Biol. 2023 Nov 20;33(22):4880-4892.e14. doi: 
            10.1016/j.cub.2023.09.064. Epub 2023 Oct 24.
  PUBMED    37879333
REFERENCE   2  (bases 1 to 5411)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cub.2023.09.064"; journalName: "Curr Biol"; date: 
            "2023-11-20- 20"; volume: "33"; issue: "22"; pages: "4880-4892.e14"
COMMENT     Sequence Label: pFPV25.1_dGFPmut3-AAV
FEATURES             Location/Qualifiers
     source          1..5411
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(10..27)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(181..769)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(261..280)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(940..1731,1732..1800))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1563..1582
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1801..1905)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1973..1991
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     CDS             2659..3372
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             2659..3372
                     /codon_start=1
                     /product="Aequorea victoria green fluorescent protein"
                     /label=GFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(2689..2717)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     3312..3332
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     oriT            4021..4108
                     /label=RSF1010 oriT
                     /note="origin of transfer of the broad-host-range plasmid 
                     RSF1010 (Scholz et al., 1989)"
ORIGIN
        1 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
       61 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
      121 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
      181 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
      241 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
      301 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
      361 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
      421 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
      481 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
      541 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
      601 taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac
      661 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
      721 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
      781 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
      841 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
      901 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
      961 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     1021 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     1081 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     1141 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     1201 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctgcagg
     1261 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     1321 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     1381 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     1441 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     1501 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaacacg
     1561 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     1621 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     1681 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     1741 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     1801 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     1861 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     1921 agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg
     1981 tatcacgagg ccctttcgtc ttcaagaatt cgagctcggt acccggggat cccggagttc
     2041 atgcgtgatg caatgaaagt gccgttctac ttcggtggga cctcactgct tatcgttgtt
     2101 gtcgtgatta tggactttat ggctcaagtg caaactctga tgatgtccag tcagtatgag
     2161 tctgcattga agaaggcgaa cctgaaaggc tacggccgtt aattggtcgc ctgagaagtt
     2221 acggagagta aaaatgaaag ttcgtgcttc cgtcaagaaa ttatgccgta actgcaaaat
     2281 cgttaagcgt gatggtgtca tccgtgtgat ttgcagtgcc gagccgaagc ataaacagcg
     2341 ccaaggctga ttttttcgca tatttttctt gcaaagttgg gttgagctgg ctagattagc
     2401 cagccaatct tttgtatgtc tgtacgtttc catttgagta tcctgaaaac gggcttttca
     2461 gcatggtacg tacatattaa atagtaggag tgcatagtgg cccgtatagc aggcattaac
     2521 attcctgatc agaaacacgc cgtgatcgcg ttaacttcga tctacggtgt cggcaagacc
     2581 cgttctaaag ccatcctggc tgcagcgggt atcgctgaaa atgttaagat cctctagatt
     2641 taagaaggag atatacatat gagtaaagga gaagaacttt tcactggagt tgtcccaatt
     2701 cttgttgaat tagatggtga tgttaatggg cacaaatttt ctgtcagtgg agagggtgaa
     2761 ggtgatgcaa catacggaaa acttaccctt aaatttattt gcactactgg aaaactacct
     2821 gttccatggc caacacttgt cactactttc ggttatggtg ttcaatgctt tgcgagatac
     2881 ccagatcata tgaaacagca tgactttttc aagagtgcca tgcccgaagg ttatgtacag
     2941 gaaagaacta tatttttcaa agatgacggg aactacaaga cacgtgctga agtcaagttt
     3001 gaaggtgata cccttgttaa tagaatcgag ttaaaaggta ttgattttaa agaagatgga
     3061 aacattcttg gacacaaatt ggaatacaac tataactcac acaatgtata catcatggca
     3121 gacaaacaaa agaatggaat caaagttaac ttcaaaatta gacacaacat tgaagatgga
     3181 agcgttcaac tagcagacca ttatcaacaa aatactccaa ttggcgatgg ccctgtcctt
     3241 ttaccagaca accattacct gtccacacaa tctgcccttt cgaaagatcc caacgaaaag
     3301 agagaccaca tggtccttct tgagtttgta acagctgctg ggattacaca tggcatggat
     3361 gaactataca aaaggcctgc agcaaacgac gaaaactacg ctgcagcagt ttaaagcttt
     3421 aatgcggtag tttatcacag ttaaattgct aacgcagtca ggcaccgtgt atgaaatcta
     3481 acaatgcgct catcgtcatc ctcggcaccg tcaccctgga tgctgtaggc ataggcttgg
     3541 ttatgccggt actgccgggc ctcttgcggg atatcgtcca ttccgacagc atcgccagtc
     3601 actatggcgt gctgctagcg cttttagccg ctttagcggc ctttccccct acccgaaggg
     3661 tgggggcgcg tgtgcagccc cgcagggcct gtctcggtcg atcattcagc ccggctcatc
     3721 cttctggcgt ggcggcagac cgaacaaggc gcggtcgtgg tcgcgttcaa ggtacgcatc
     3781 cattgccgcc atgagccgat cctccggcca ctcgctgctg ttcaccttgg ccaaaatcat
     3841 ggcccccacc agcaccttgc gccttgtttc gttcttgcgc tcttgctgct gttcccttgc
     3901 ccgcacccgc tgaatttcgg cattgattcg cgctcgttgt tcttcgagct tggccagccg
     3961 atccgccgcc ttgttgctcc ccttaaccat cttgacaccc cattgttaat gtgctgtctc
     4021 gtaggctatc atggaggcac agcggcggca atcccgaccc tactttgtag gggagggggc
     4081 acttaccggt ttctcttcga gaaactggcc taacggccac ccttcgggcg gtgcgctctc
     4141 cgagggccat tgcatggagc cgaaaagcaa aagcaacagc gaggcagcat ggcgatttat
     4201 caccttacgg cgaaaaccgg cagcaggtcg ggcggccaat cggccagggc caaggccgac
     4261 tacatccagc gcgaaggcaa gtatgcccgc gacatggatg aagtcttgca cgccgaatcc
     4321 gggcacatgc cggagttcgt cgagcggccc gccgactact gggatgctgc cgacctgtat
     4381 gaacgcgcca atgggcggct gttcaaggag gtcgaatttg ccctgccggt cgagctgacc
     4441 ctcgaccagc agaaggcgct ggcgtccgag ttcgcccagc acctgaccgg tgccgagcgc
     4501 ctgccgtata cgctggccat ccatgccggt ggcggcgaga acccgcactg ccacctgatg
     4561 atctccgagc ggatcaatga cggcatcgag cggcccgccg ctcagtggtt caagcggtac
     4621 aacggcaaga ccccggagaa gggcggggca cagaagaccg aagcgctcaa gcccaaggca
     4681 tggcttgagc agacccgcga ggcatgggcc gaccatgcca accgggcatt agagcgggct
     4741 ggccacgacg cccgcattga ccacagaaca cttgaggcgc agggcatcga gcgcctgccc
     4801 ggtgttcacc tggggccgaa cgtggtggag atggaaggcc ggggcatccg caccgaccgg
     4861 gcagacgtgg ccctgaacat cgacaccgcc aacgcccaga tcatcgactt acaggaatac
     4921 cgggaggcaa tagaccatga acgcaatcga cagagtgaag aaatccagag gcatcaacga
     4981 gttagcggag cagatcgaac cgctggccca gagcatggcg acactggccg acgaagcccg
     5041 gcaggtcatg agccagaccc agcaggccag cgaggcgcag gcggcggagt ggctgaaagc
     5101 ccagcgccag acaggggcgg catgggtgga gctggccaaa gagttgcggg aggtagccgc
     5161 cgaggtgagc agcgccgcgc agagcgcccg gagcgcgtcg cgggggtggc actggaagct
     5221 atggctaacc gtgatgctgg cttccatgat gcctacggtg gtgctgctga tcgcatcgtt
     5281 gctcttgctc gacctgacgc cactgacaac cgaggacggc tcgatctggc tgcgcttggt
     5341 ggcccgatga agaacgacag gactttgcag gccataggcc gacagctcaa ggccatgggc
     5401 tgtgagcgct c
//
