LOCUS       sequence-370388-       11621 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  In vivo gene amplification (HamAmp) of nerolidol synthetic module at
            RPL25 locus (15 copy). .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11621)
  TITLE     Vickers Lab plasmids
REFERENCE   2  (bases 1 to 11621)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pINER5
FEATURES             Location/Qualifiers
     source          1..11621
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     147..166
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     400..417
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    534..555
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(570..575,576..593,594..600)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    608..624
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     613..635
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     632..648
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     632..648
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             complement(3856..4230)
                     /codon_start=1
                     /product="small protein tag, derived from photoactive 
                     yellow protein, that allows in vivo fluorescent labeling by
                     addition of a cell-permeant fluorogen (Plamont et al., 
                     2016)"
                     /label=Y-FAST
                     /translation="MEHVAFGSEDIENTLAKMDDGQLDGLAFGAIQLDGDGNILQYNAA
                     EGDITGRDPKQVIGKNFFKDVAPGTDSPEFYGKFKEGVASGNLNTMFEWMIPTSRGPTK
                     VKVHMKKALSGDSYWVFVKRV"
     promoter        complement(7166..7607)
                     /gene="S. cerevisiae GAL1"
                     /label=GAL1 promoter
                     /note="inducible promoter, regulated by Gal4"
     primer_bind     complement(7172..7195)
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     protein_bind    7490..7607
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     primer_bind     complement(9653..9670)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9653..9669)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9662..9684)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(9878..9897)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9997..10019
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(10057..10075)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        10143..10247
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10248..10316,10317..11108)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10466..10485)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(11279..11621,1..246)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
       61 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
      121 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
      181 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
      241 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
      301 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
      361 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
      421 gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc
      481 agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg
      541 agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg
      601 tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgcc
      661 aagcttgttt aaactatgtt ccttgatacc tcgacgtgga gcacgaaata gaagaaaaaa
      721 gtataatgag gatggaataa ttttcttgaa tctgagcaga aaaacgaacg aacactctaa
      781 tggatttgtt aatggctgtt ggtagtaaaa agaacaactt ccacagcatc aacgcttcta
      841 tcaaaacgat tgctaataaa ctgttgagcc cgataattac gttgctcacc atatcttaat
      901 caagtcctta atgcactatt cttgtcgtcg gatagtttat ttacgtgtgg ttttcgtatc
      961 tcatcaatat caactctact agttcccatc tatcttcctc tcaattttct cgtgctatta
     1021 atattgcagt tcacatacca gatggagcca ttggcacaaa tgtggtttcc tttcttattg
     1081 ctgtatccaa cttttgtgaa gctacaggtg cgacaaacat aaaaaagttc ttgccgttaa
     1141 gaagctgaag ttgcttctcg ctataccaga ggggattatt gtcgggagtc atgatgcgac
     1201 atttacccgg ttgagtaagg aaggggtgga ggaatcagaa gagagataaa caaacataca
     1261 cgtcaaaaca gtatagttca agtaacacat atagacatgt acacgcgcga ttacgtgtgt
     1321 acgtgcacat gttaatgtta tatacatata tagatataga tatataattg caataatgaa
     1381 ctacaaatta ttataccccc gttctttttt ttttcttttt tttagtaatt tttctttaga
     1441 tttatcggaa tacttacttt ggtgcttcac cttcggcggc agcaggtggc tgctgttgct
     1501 gatgttgttg ttgttgttgt tggtcttcag cttgaccgga ctcggacatg tctgaagtcc
     1561 ataaggttaa attgtccctt agcagttgca tgataagtgt gctatctttg tatgattctt
     1621 cagacagagt gtccaactca gcaatagcgt cgtcaaaagc ttgcttggcc aaatggcagg
     1681 ctttgtctgg agagttttga atttcgcatg caaatcatta cgaccgagat tcccgggacg
     1741 ccacagaaac ctcagaataa gctaaagggt aatataataa agcccttcga ttcaataata
     1801 gcattaccca agaggcatag cggcaaacta agttgacaac acaaaatcaa atcgcttcat
     1861 ttttatttta ttaatgatat tttattagag aggaaattat gtaatatcta gatattaaat
     1921 ataaagtaga tctctgtcta aaatattctt aacaaaatct tatattcgaa aggatgacgg
     1981 tccaatcaaa ggattgtata agagaaatta gttttcgaaa tcaattttta cttgacctga
     2041 acttaacgat ttgctctcaa tccgccatgg tcactattat aaagatgtgt tatagatcat
     2101 tgccttcatg tgttcttcta aaattggcaa gttatggttg tcatcgtaag agtacatcat
     2161 agggatcatt ctagccatat tcaaggaacc tattctgaat gattcagaaa aagggtttgg
     2221 tgataagcat tccttattca aacatttcca tgcttcggag atcattctta ttacttcttc
     2281 tctggctgaa tcgacactgg aacccttatg ttccttcatg taacattcga tgtaagaacc
     2341 atcataacca tcttggtctt catctttggc tgaacccaag tcatcagata atctcaaaat
     2401 ggttgcgata cttgtggaaa tacctggata gtcatctacc aaatcaactg attcttgtgt
     2461 aataccgtca cctaacaaga aaaacatgtg ggccaagaca acatgaacac ctgaagagat
     2521 gataccgttc tttaagtatt cttctgcatt tggcaagtga ccagaggcga accactttgc
     2581 ttcaactaaa aatgcattgc acaaactagc ccaagtcttt tgcaaggaat caatagggtt
     2641 ttgaccatgc tttttataga ttttgtatgc gatttcgttg gtaacatcgt acaaagcctt
     2701 gaaacatact ttcatgtagt ctggtaattg ttcgacggca gacaattccc atctattaac
     2761 tgcatcagta aacaaggtca attcttcaat ggtaccatag acgtcgaaga tgtcatctat
     2821 gatgtatata aaagaaatag gcttagttaa ttcaactctt tcttcactga atcttggatc
     2881 ggttaaagcg gccataggcc acatatacca cttcaatggt tggtttctag ccaatttcaa
     2941 ttcattggct aaacctgtat ccttccacca ttgagaaact tggaatactt ccttttgatg
     3001 gatactttgt acgatagtca aatccatctt agctaattct tgcaaattat cgacaccgtc
     3061 ccagtttgga cccttgcagt tcatgtagga caatttttga cccataaagg tagccaatga
     3121 cttgtggtat ggatgtgtta aagtgttaga aaccaatctg gcttcgtgat gattaggatc
     3181 ttttgtccat ctacctaaca attgactgga gaaatcagca gcttgatcta agatgtcttc
     3241 accttctatt ctcaattgac ttgcttcgta taaggacatc aaacctctaa tatctgttga
     3301 caattctgat ctgaatctac cttccttatc cttgaagtta ttaaagacgt cagcggtaac
     3361 attgtgacct tcttgtctta agaatctaaa caatgttgaa acttcgtaca aaccatgttc
     3421 agggtaacca tctggagttg aagatatgat ttgttgcttt tgcaagaatg caccaatttc
     3481 acctctgaaa tggtaatcga tacccaatct ttgcaaagcg tctatcataa ctaaaccttc
     3541 caaagggttt tcttcaactt ctcttaatgc ttttctaaca tcttccaact tttcttcgta
     3601 cttaacgtat aattcgtcag aaaatgatct gtaaccagtt tgagggttat tgtgcttact
     3661 aggattggaa atacatgtca aatcttcagc tatagaccat ttatgagtaa ttggtacgga
     3721 aggagcgatt ggagttctgt aagcgttgga gttttgtggg gagttgttag ttgcgatagg
     3781 acctgctgcg gtagctggac ctgggttaag ttcaacgtca ccagccaact tcaacaaaga
     3841 gaagttagta gcaccaactc ttttaacgaa aacccagtaa gagtcaccag ataaagcctt
     3901 tttcatgtga actttaacct tagttggacc tctggaagtt gggatcatcc attcaaacat
     3961 agtgtttaag ttaccagaag caacaccttc cttaaactta ccatagaact ctggagagtc
     4021 agtacctgga gcaacgtcct taaagaagtt cttaccaata acttgttttg gatctctacc
     4081 agtaatatca ccttcagcag cattgtattg caaaatgtta ccatcaccgt ccaattgaat
     4141 agcaccgaaa gctaaaccgt ctaattgacc atcatccatt ttagccaaag tgttttcaat
     4201 atcttcagaa ccgaaagcaa cgtgttccat tttggatcca ctgtaaaaac tttttttatt
     4261 atactatttt ctattgttta atagtcgttg ttcttgtgga tatatgaaca agtaatcaaa
     4321 aaagttatca caaatgaact cagatatatt tcagccgaat ggctagagtt tatatatgtc
     4381 tgtctaaccc acactttttc cgcattttat tcctcaactc atccctccct tgaacaacga
     4441 ctgctaacaa cgtctcactc taaaatcttt acacgtttag ctttacagga ggagcataat
     4501 tgacttttgt tcctatttca agttgggaag ggcttacttt agcattagct cggagtggtc
     4561 caccccggaa ctatggcctg cggaaatact ctcacaggca catggcccct gaactgcggg
     4621 gagtgtgccg cacggaagag tgagcgccgg tgcctaccgg aagaacccct tcctcagtat
     4681 ttgagcacgt gactcgaatg ctggccgcaa aaaagaaaaa agaatgtaat ccggttgagc
     4741 cgccccggaa gacgcttttc cggtagttaa ttctttatac attactggaa cagattctgc
     4801 tccgtttaat tagacttgga tgcaaggttg cctgtgatag ctttagtggg tctattctac
     4861 ggccttctaa tggcaccgtt atgacctgga tctggtacgt ctgtacgatt agcggggcaa
     4921 gttcaaataa aattggttta ctcgagttaa tttatctcat gaagtaatga tacctcagcg
     4981 atgtgctcgc tcgttagtat gcttcataaa ttcattatta tttttttttg gaagtgtttt
     5041 tcgacaaaag ttgcattttt acgaaggata tgtaaactca agaagtacta aataaatata
     5101 aatacaattc gaaaatttga aaaaagtagg agacaacact ttccttcctc acgaagaagt
     5161 taagcttgag catcggaccg aagcataaga atgcagcaaa ttgataatat tcccgttaag
     5221 ccagtcgtag gaaaaggaaa agaatgagta gcaaatgtca cacaacaaac gcgcaccatc
     5281 cctacataca caaacacaca cacacacaca catatatata tattctaccc catataagcg
     5341 agtgattcat catgaaaaga atttatcact tcgaatttaa ataataacat tcttacctga
     5401 tgtacaactc tttactctca tcttgcgaaa agaaaagggc agggtgccgc tgaaacagcg
     5461 gcgttgctga tgaaaataac cctgtgtttt cgcctcattc tctcaaaacc ttcacagatc
     5521 cttttcttgt tatagcctca ataaaatcac agtacactaa cacgtaatta taaatagtaa
     5581 ccggaaccct cgctgcaata cgcaaattgg tacctacttt gtaaggcaca ggaaaccccg
     5641 tgcactctgc cactacactt tatttacgct taatcatgaa tttaacatgt tatgaaagat
     5701 ttattgtata tttaaagaaa aatttaaaaa taatattaaa tttattaatt aaaccaatta
     5761 gagctagggg cccattgtag caaagattgt aaggaaatag caaaccagag gtccaagtca
     5821 aaacggcaaa aaagaagaaa gtaagcaata taatggacgg gtttacatgt ttaaatatat
     5881 gtacatgtat gtagtcttat actttcaaaa cataatcgca tgcgggtcat caaaataaac
     5941 ctccattttg gacttgggaa aaagaaaatt tgatttaaac tgttgtatgt agctaacaat
     6001 acataaaact atttaaataa aataggaata agaatgctca attaaaaaat gatttatttt
     6061 ctaacaaaac ttcctgcagg tcactattat ttgcttctct tgtaaacttt gttcaaaaac
     6121 gcagttaaga cgtcggcttt gaagccacga gactcgtcga cttgggagat cttggccttc
     6181 aaatccttgg caacagactc ttcatattcg tggtataact ggtcgatttt caagtcattg
     6241 aaaatctttt tgcatttggc ttctgcgact gagtccttct taccgtaatt ttcgtctaaa
     6301 gtctttcttt gttctgcgga agcaagttct aatgccttgt tgattaccca agaacatttg
     6361 ttatcttgga tatctgtacc gatcttaccg atctgttctg gggtaccgaa gcagtctaag
     6421 tagtcatctt gaatttggaa atattcaccc aatggaatca agacatctct ggcttgtttc
     6481 aaatcctttt catctgtgat accggcaacg tacatagcca atgcgacagg caagtagaaa
     6541 gaatagtaag cagtcttgaa agtaactatg aaggagtgct tctttaggga gaacttactc
     6601 aagtcgactt tgtcttcagg tgcagtgatt aagtccatca attggcccaa ttcggtttgg
     6661 aaggtgactt catggaacaa ttcggtgata tctatgtagt atttttcgtt tctgaagtga
     6721 gatttcaaaa gcttgtagat agcagcctct aacatgaatg cgtcattgat ggcaatttcc
     6781 ccaacttcag gaaccttgta ccaacatggt tggcctcttc tggtaatgga cttgtccatc
     6841 atatcatcgg cgaccaagaa gtaagcctgc aacaactcaa tgcaccaacc tagaatagca
     6901 accttttcgt attcttcttg ccccaattgt tcaacggtct tgttggagag aatagcatac
     6961 gtgtccacaa cggacaaacc tctatttaac ttaccgcctg gagtgttgta gttcaatgag
     7021 tgggcatacc agtcacatgc ttccttaggc ataccgtaag ccaaaagcga tgcgttcaat
     7081 tcctctacta atttagggaa aacgttcaag aatctctctc tcctaatttc tttttctgaa
     7141 gccattttgc tagctatagt tttttctcct tgacgttaaa gtatagaggt atattaacaa
     7201 ttttttgttg atacttttat gacatttgaa taagaagtaa tacaaaccga aaatgttgaa
     7261 agtattagtt aaagtggtta tgcagctttt gcatttatat atctgttaat agatcaaaaa
     7321 tcatcgcttc gctgattaat taccccagaa ataaggctaa aaaactaatc gcattattat
     7381 cctatggttg ttaatttgat tcgttgattt gaaggtttgt ggggccaggt tactgccaat
     7441 ttttcctctt cataaccata aaagctagta ttgtagaatc tttattgttc ggagcagtgc
     7501 ggcgcgaggc acatctgcgt ttcaggaacg cgaccggtga agaccaggac gcacggagga
     7561 gagtcttccg tcggagggct gtcgcccgct cggcggcttc taatccgtac ttcaatatag
     7621 caatgagcag ttaagcgtat tactgaaagt tccaaagaga aggttttttt aggctaagat
     7681 aatggggctc tttacatttc cacaacatat aagtaagatt agatatggat atgtatatgg
     7741 tggtattgcc atgtaatatg attattaaac ttctttgcgt ccatccaaaa aaaaagtaag
     7801 aatttttgaa aattcaatat aagctagcgc aggctaaccg gaacctgtat tatttagttt
     7861 atgctacgtt aaataaagac ctttcgttca cataactgaa tgtgtaatgg ccttgagatt
     7921 tcaagcatac caagttggtg gagacggggt cgttacaaaa gactctttaa gccaagattt
     7981 ccttgacagc cttggcgata gcatcgccaa cctcagtggt agagttagaa ccaccaaggt
     8041 caccggttct gacacctgca tccaagacat ttctaacagc ttcttcaaga gccctacctt
     8101 cttcaaccaa atccaaggat aacttcaaca tcatagctgc agataagatg gtagcaattg
     8161 ggttaacctt gtttgctggt aaatctgggg cagaaccatg acatggttcg tacaaaccga
     8221 atgccttgtt agtgtcaggt agggaagcta gagatgcaga aggtaataaa cccaaagaac
     8281 ctggaataac agaggcttca tcggagataa tatcaccaaa catgttgttg gtaataacaa
     8341 caccgtttag cttagttggt gatttaacca aaatcatagc agcagagtcg atcaattggt
     8401 gctgaacagt taattgtggg aactcagtct tgatggtttc ttcaacagtc tttctccaca
     8461 atctggaaga ggcaagcacg ttagccttgt caagagacca gattggtaat ggtgggtttt
     8521 gttgcaatgc caagaaagca gccattcttg taattctttg aacttcagga acactgtatt
     8581 tctcagagtc ccaagcaact ccgtcacctt catcttcttt tctttcacca aagtagatac
     8641 caccaaccaa ttctctaacg acgacgaaat cggtaccctt tgcatattca ggcttcaaag
     8701 gagaaagatc tagtaaagaa tcagaagcaa agttacatgg tctcaagttg gcgtatagac
     8761 ccaattcctt tctgatcttc aatagacctt gttctggtct aactgcgccc gtaccccatt
     8821 ttggaccacc aacagcacct agtaagacag catcggcttt cttagaggct tctagagctt
     8881 catctggtaa aggagtgcca gtggcatcga tggcagcacc cccgatcaag tgatgttgga
     8941 aattgaactt aatttctgga cggacttcag caatggcatt caagacctta atagcttcgt
     9001 cagtaacttc tttaccgacg tgatcacccg gtaggacaac gatattctta gacattttgc
     9061 ggccgcttta tcttattgat cttctttgtt tagccttttc aacagcagaa gaaaagcata
     9121 tgagatacat ttaaaattgt tttaaaataa ttttaaaaga taatatctaa aagattgtat
     9181 gcatacgaac gaagttgcat tgggaagccc caacgcagtg aggcaagccg aaggtagaaa
     9241 gaaaatcccc ttgtgcgtcc agtgagaaac agcaagcagt gggtaggaag gcccagttta
     9301 gcctgactgt tccaacgggg ctcccaggct gaggaaatgc ttctctccgt aatgggagag
     9361 cctaactcca gagcgtggac ttcggctcac ggttttctct ttttttggcg cacaatgact
     9421 gctgagacaa aaaaagaaag caactccatg taaacacccg tacatattaa ataagtgacc
     9481 catgcctcct catctcttca ttgcaaagta cggaaaccgt tagattggta ccaacaaggt
     9541 tgctatatcg ggcatacaag ataagaaact taaagaacgc cgagacaaga atatgtaatg
     9601 ataagcctcc tccggggtgt tagactgatt agtacatgtt taaactgaat tcactggccg
     9661 tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag
     9721 cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc
     9781 aacagttgcg cagcctgaat ggcgaatggc gcctgatgcg gtattttctc cttacgcatc
     9841 tgtgcggtat ttcacaccgc atatggtgca ctctcagtac aatctgctct gatgccgcat
     9901 agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc
     9961 tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt
    10021 tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc ctatttttat
    10081 aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg
    10141 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
    10201 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
    10261 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
    10321 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
    10381 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
    10441 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
    10501 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
    10561 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
    10621 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
    10681 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
    10741 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
    10801 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
    10861 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
    10921 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
    10981 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
    11041 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
    11101 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
    11161 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
    11221 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
    11281 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
    11341 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
    11401 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
    11461 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
    11521 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
    11581 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc c
//
