LOCUS       sequence-37039-e         258 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 258)
  AUTHORS   Zhu C, Smith T, McNulty J, Rayla AL, Lakshmanan A, Siekmann AF, 
            Buffardi M, Meng X, Shin J, Padmanabhan A, Cifuentes D, Giraldez AJ,
            Look AT, Epstein JA, Lawson ND, Wolfe SA
  TITLE     Evaluation and application of modularly assembled zinc-finger 
            nucleases in zebrafish.
  JOURNAL   Development. 2011 Oct;138(20):4555-64.
  PUBMED    21937602
REFERENCE   2  (bases 1 to 258)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Development."; date: "2011-10"; volume: "138(20)"; pages: "4555-64"
COMMENT     Sequence Label: pBS-F1-GTC
FEATURES             Location/Qualifiers
     source          1..258
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     8..24
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8..24
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     43..63
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        45..63
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 tttcacacag gaaacagcta tgaccatgat tacgccaagc gcgcaattaa ccctcactaa
       61 agggaacaaa agctggagct ccaccgcggt ggcggccgct ctagattact tttcacctgt
      121 atggattttg gtatgcctct tgcgttcatc acncctggca aacttcctcc cacaaatgtc
      181 gcatgcaaaa ggcttctcgc ctgtgtgggt cctgatgtgg gtggtcaggt ggctggagcg
      241 actgaagtta cgcatgca
//
